FastQCFastQC Report
Sat 18 Jun 2016
SRR3550294_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550294_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences283248
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTC12220.4314240524204937No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGC10480.36999378636389313No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCG9470.334335988250579No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7520.26549172456645764No Hit
GCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGC5910.20865107608879851No Hit
GAACTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCT3070.10838558436423205No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT351.2075907E-745.0000046
CCTGTTA207.024276E-445.023
ACGGGTA406.7830115E-945.05
TCGGAAG207.024276E-445.01
TGCGATG253.883338E-545.01
GGTATCA253.883338E-545.08
GTGTACG253.883338E-545.01
CGTAAGG253.883338E-545.02
TCGCATG302.1596898E-644.9999961
TTAGGGT451.9195795E-840.04
GCGATTA451.9195795E-840.09
ACGGGCC403.44593E-739.3755
TTACGGG800.039.3753
ATAGCGG356.2316267E-638.571432
TACGGGT356.2316267E-638.571434
TATACGG356.2316267E-638.571432
TGGTCAA1000.038.2514
TAAGGGA1500.037.5000044
AAGGGTC900.037.55
TCAAGCG900.037.517