##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3550294_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 283248 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.04201265322262 33.0 31.0 34.0 30.0 34.0 2 32.12377492515393 33.0 31.0 34.0 30.0 34.0 3 32.02867804891826 33.0 31.0 34.0 30.0 34.0 4 35.72811811557363 37.0 35.0 37.0 33.0 37.0 5 33.407116731627404 37.0 35.0 37.0 30.0 37.0 6 34.50060018076032 37.0 35.0 37.0 30.0 37.0 7 35.70120883466079 37.0 35.0 37.0 32.0 37.0 8 35.57279839575213 37.0 35.0 37.0 33.0 37.0 9 37.51237078461278 39.0 37.0 39.0 35.0 39.0 10 37.134835903519175 39.0 37.0 39.0 34.0 39.0 11 37.08270490877253 39.0 37.0 39.0 34.0 39.0 12 37.10762653222618 39.0 37.0 39.0 34.0 39.0 13 37.180128368073206 39.0 37.0 39.0 34.0 39.0 14 38.353082810823025 40.0 38.0 41.0 34.0 41.0 15 38.46108357340564 40.0 38.0 41.0 34.0 41.0 16 38.28849276958707 40.0 37.0 41.0 34.0 41.0 17 38.25468494040558 40.0 37.0 41.0 34.0 41.0 18 38.11735299101847 40.0 37.0 41.0 34.0 41.0 19 37.92469143647969 40.0 37.0 41.0 33.0 41.0 20 38.18994308874202 40.0 37.0 41.0 34.0 41.0 21 38.14907430943908 40.0 37.0 41.0 34.0 41.0 22 38.28234974298142 40.0 37.0 41.0 34.0 41.0 23 38.3255345139242 40.0 37.0 41.0 34.0 41.0 24 38.19458566344687 40.0 37.0 41.0 34.0 41.0 25 37.824609529458286 40.0 36.0 41.0 33.0 41.0 26 37.93667033836073 40.0 36.0 41.0 34.0 41.0 27 37.99595760605547 40.0 36.0 41.0 34.0 41.0 28 37.89368680449641 40.0 36.0 41.0 33.0 41.0 29 37.878668163588095 40.0 36.0 41.0 34.0 41.0 30 37.66736923120375 40.0 36.0 41.0 33.0 41.0 31 37.5628954132068 40.0 36.0 41.0 33.0 41.0 32 37.55180619104107 40.0 36.0 41.0 33.0 41.0 33 37.50093204541603 40.0 36.0 41.0 33.0 41.0 34 37.42476557645597 40.0 36.0 41.0 33.0 41.0 35 37.395332005874714 40.0 35.0 41.0 33.0 41.0 36 37.272460882336325 40.0 35.0 41.0 33.0 41.0 37 37.243867564819524 40.0 35.0 41.0 33.0 41.0 38 37.067848669716994 40.0 35.0 41.0 32.0 41.0 39 36.94953538948201 39.0 35.0 41.0 32.0 41.0 40 36.8076102920409 39.0 35.0 41.0 31.0 41.0 41 36.647026633903856 39.0 35.0 41.0 31.0 41.0 42 36.66114500367169 39.0 35.0 41.0 31.0 41.0 43 36.58983999887025 39.0 35.0 41.0 31.0 41.0 44 36.51224368751059 39.0 35.0 41.0 31.0 41.0 45 36.44773131672598 39.0 35.0 41.0 31.0 41.0 46 36.31054411681636 39.0 35.0 41.0 31.0 41.0 47 36.18870389199571 38.0 35.0 41.0 30.0 41.0 48 36.120410382421056 38.0 35.0 40.0 30.0 41.0 49 36.05371970852398 38.0 35.0 40.0 30.0 41.0 50 35.966979466757046 38.0 35.0 40.0 30.0 41.0 51 34.61997613398859 37.0 34.0 40.0 26.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 13.0 9 18.0 10 14.0 11 10.0 12 6.0 13 13.0 14 8.0 15 12.0 16 22.0 17 37.0 18 85.0 19 140.0 20 249.0 21 387.0 22 576.0 23 875.0 24 1152.0 25 1646.0 26 2087.0 27 2467.0 28 2804.0 29 3299.0 30 4053.0 31 5289.0 32 6923.0 33 9984.0 34 17748.0 35 22514.0 36 23098.0 37 35523.0 38 57514.0 39 84653.0 40 27.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.859275264079535 18.80366322092301 22.53290402756595 13.804157487431508 2 31.32449302378128 25.19241089080947 25.378819974015705 18.10427611139355 3 33.32027057560865 24.757103315822178 25.9638196915777 15.95880641699147 4 29.029331186804498 25.215358978704174 26.607425295147713 19.147884539343615 5 24.8630175676439 34.486033440659774 22.668474269897757 17.982474721798567 6 26.58024063718014 35.52575834604305 23.443413545726713 14.450587471050106 7 80.12801502570186 6.078065864542733 9.222660001129752 4.571259108625656 8 81.07630062701236 6.401104332598995 7.832711969722646 4.689883070665989 9 75.04519008077727 7.436592667909394 10.012780319719822 7.505436931593515 10 41.26172117720161 23.903434446139073 18.074973168389537 16.759871208269786 11 31.91584759645258 25.197706603400555 23.584279500649608 19.302166299497262 12 27.737530362085522 23.142970118059086 28.013966559340226 21.105532960515166 13 25.443074620120882 23.201576004067107 30.394565892786535 20.960783483025473 14 21.799271309947464 27.323405637462578 29.174786759306333 21.702536293283625 15 21.534132632887083 24.85383833248602 33.192820425916516 20.419208608710388 16 24.22576681918319 24.19081511608202 29.69553183076315 21.887886233971642 17 23.86671750550754 23.963099474665313 28.54812743602779 23.622055583799355 18 25.31774275546518 24.135033610122576 28.34018245495114 22.20704117946111 19 25.700446252047676 25.88120657515675 26.343345760605548 22.075001412190023 20 26.612014912726657 25.754109472970683 26.877859684799187 20.756015929503473 21 25.20123707846128 26.50327628085635 27.74565045472519 20.549836185957183 22 24.765223408461843 24.308732983110207 26.53646274642716 24.38958086200079 23 24.07995819917528 26.528342653787497 26.169646387617917 23.222052759419306 24 23.627351296390444 25.576879624922327 27.194543297746144 23.601225780941085 25 23.336087103880697 27.22737671581088 26.189770095464045 23.246766084844378 26 22.54773202282099 27.466036829915836 26.8008953284754 23.185335818787774 27 23.972631757329268 26.213071230864827 26.682271366435067 23.13202564537084 28 21.247105010450205 27.399663898774218 27.85721346664407 23.496017624131504 29 23.671835282155566 26.342992713099473 26.616604530305597 23.36856747443936 30 23.251355702423318 27.053677342823253 27.53841156866068 22.15655538609275 31 24.949867254137718 27.407783991413886 24.697791334802012 22.94455741964639 32 25.5772326724284 27.340351917754052 24.912797266000116 22.16961814381743 33 24.153745127944415 27.027198779867817 24.911385075975822 23.907671016211943 34 23.848359035191773 28.068688922781448 25.960642264023047 22.12230977800373 35 23.501666384228663 26.674504321301473 25.91721742077614 23.906611873693727 36 23.09707394226967 29.906301191888378 24.74439360560357 22.25223126023838 37 24.709088854996327 27.597723549680847 26.38500536632209 21.308182229000735 38 23.39186860984014 28.564367621307124 24.039357735976953 24.00440603287578 39 25.788355081059706 25.478732418234195 24.900087555781507 23.83282494492459 40 24.054185731231996 25.51756764390216 27.182892730045754 23.245353894820088 41 22.17491385640852 26.513867706038525 26.212012088346608 25.099206349206348 42 22.8164011749421 26.076794893520873 26.76453143534994 24.342272496187086 43 23.51296390442298 25.53486697169971 26.636375190645655 24.315793933231657 44 23.051883861492403 25.83672258939163 26.524459131220695 24.58693441789527 45 23.289837880585214 25.02789075297972 26.790656950799303 24.891614415635768 46 24.15692255549907 26.293213014743266 26.062672993277975 23.487191436479694 47 22.734141106027227 25.766113088177146 27.927469920352483 23.572275885443144 48 22.806162797266 25.239366209117097 27.095336948539796 24.859134045077106 49 22.900426481387335 24.946689826583064 27.979014856239054 24.173868835790543 50 22.44817262610857 24.6377732587697 28.1513020392024 24.762752075919337 51 22.46017624131503 24.39699485962831 26.259320454160314 26.883508444896346 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 121.0 1 285.0 2 449.0 3 1014.0 4 1579.0 5 1131.0 6 683.0 7 652.0 8 621.0 9 637.0 10 653.0 11 653.5 12 654.0 13 677.5 14 701.0 15 698.0 16 695.0 17 703.0 18 711.0 19 687.5 20 664.0 21 807.0 22 950.0 23 1056.0 24 1162.0 25 1257.5 26 1678.0 27 2003.0 28 2459.5 29 2916.0 30 3538.5 31 4161.0 32 4493.5 33 4826.0 34 5798.0 35 6770.0 36 7207.0 37 7644.0 38 8548.0 39 9452.0 40 10530.0 41 11608.0 42 12731.0 43 13854.0 44 15690.5 45 17527.0 46 21608.5 47 25690.0 48 25091.0 49 24492.0 50 24006.5 51 23521.0 52 21397.0 53 19273.0 54 17893.5 55 16514.0 56 15610.5 57 14707.0 58 14093.5 59 13480.0 60 13163.0 61 12846.0 62 11962.0 63 11078.0 64 9722.5 65 8367.0 66 7189.5 67 6012.0 68 5298.5 69 4585.0 70 4070.5 71 3556.0 72 3080.5 73 2605.0 74 2244.0 75 1564.5 76 1246.0 77 1003.0 78 760.0 79 604.5 80 449.0 81 344.0 82 239.0 83 175.0 84 111.0 85 80.0 86 49.0 87 33.5 88 18.0 89 12.0 90 6.0 91 3.5 92 1.0 93 1.5 94 2.0 95 1.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 283248.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.459265228480895 #Duplication Level Percentage of deduplicated Percentage of total 1 67.23947929417307 25.187414887053784 2 10.390006079618951 7.784039869239505 3 4.5566192430153425 5.120628263079346 4 3.075976969896801 4.608953486082531 5 2.397721618442643 4.490844502465271 6 1.8602961364202715 4.181119582661113 7 1.6500093490833156 4.326569648574952 8 1.4228272953108825 4.263845202349796 9 1.2614130277891349 4.252644465355291 >10 6.081882883072129 32.02210092168124 >50 0.03985506448590662 0.9364475127677365 >100 0.018931155630805645 1.1234384270350288 >500 0.0029891298364429966 0.8547089694053872 >1k 0.0019927532242953313 0.8472442622490084 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAATCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTC 1222 0.4314240524204937 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGC 1048 0.36999378636389313 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCG 947 0.334335988250579 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 752 0.26549172456645764 No Hit GCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGC 591 0.20865107608879851 No Hit GAACTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCT 307 0.10838558436423205 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.530475060724171E-4 0.0 0.0 0.10414901429136304 0.0 2 3.530475060724171E-4 0.0 0.0 0.5090945037564255 0.0 3 3.530475060724171E-4 0.0 0.0 0.6923261594080099 0.0 4 3.530475060724171E-4 0.0 0.0 1.03266395526182 0.0 5 3.530475060724171E-4 0.0 0.0 2.1698299723210757 0.0 6 3.530475060724171E-4 0.0 0.0 2.885457267129865 0.0 7 3.530475060724171E-4 0.0 0.0 3.567545048861775 0.0 8 3.530475060724171E-4 0.0 0.0 4.648929559961588 0.0 9 3.530475060724171E-4 0.0 0.0 5.122719313110772 0.0 10 3.530475060724171E-4 0.0 0.0 6.378862339716433 0.0 11 3.530475060724171E-4 0.0 0.0 7.429884765294018 0.0 12 3.530475060724171E-4 0.0 0.0 8.642955996158843 0.0 13 3.530475060724171E-4 0.0 0.0 9.026718635259561 0.0 14 3.530475060724171E-4 0.0 0.0 9.185943060498222 0.0 15 3.530475060724171E-4 0.0 0.0 9.490270010732644 0.0 16 3.530475060724171E-4 0.0 0.0 10.015604699768401 0.0 17 3.530475060724171E-4 0.0 0.0 10.594602609727165 0.0 18 3.530475060724171E-4 0.0 0.0 11.233971643224312 0.0 19 3.530475060724171E-4 0.0 0.0 11.662924363102299 0.0 20 3.530475060724171E-4 0.0 0.0 12.054807094842682 0.0 21 3.530475060724171E-4 0.0 0.0 12.57590521380557 0.0 22 3.530475060724171E-4 0.0 0.0 13.070524769813026 0.0 23 3.530475060724171E-4 0.0 0.0 13.570086990905496 0.0 24 3.530475060724171E-4 0.0 0.0 13.947847822402982 0.0 25 3.530475060724171E-4 0.0 0.0 14.308662373608993 0.0 26 3.530475060724171E-4 0.0 0.0 14.675125684912162 0.0 27 3.530475060724171E-4 0.0 0.0 15.018993955826696 0.0 28 7.060950121448342E-4 0.0 0.0 15.358272609162288 0.0 29 7.060950121448342E-4 0.0 0.0 15.718734112862228 0.0 30 7.060950121448342E-4 0.0 0.0 16.185815963396035 0.0 31 7.060950121448342E-4 0.0 0.0 16.58228831271536 0.0 32 7.060950121448342E-4 0.0 0.0 16.967110094334295 0.0 33 7.060950121448342E-4 0.0 0.0 17.36358244365362 0.0 34 7.060950121448342E-4 0.0 0.0 17.74240241766932 0.0 35 7.060950121448342E-4 0.0 0.0 18.125105914251822 0.0 36 7.060950121448342E-4 0.0 0.0 18.508515505846468 0.0 37 7.060950121448342E-4 0.0 0.0 18.907106140202227 0.0 38 7.060950121448342E-4 0.0 0.0 19.27886516409648 0.0 39 0.0010591425182172513 0.0 0.0 19.646387617917867 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGTAT 35 1.2075907E-7 45.000004 6 CCTGTTA 20 7.024276E-4 45.0 23 ACGGGTA 40 6.7830115E-9 45.0 5 TCGGAAG 20 7.024276E-4 45.0 1 TGCGATG 25 3.883338E-5 45.0 1 GGTATCA 25 3.883338E-5 45.0 8 GTGTACG 25 3.883338E-5 45.0 1 CGTAAGG 25 3.883338E-5 45.0 2 TCGCATG 30 2.1596898E-6 44.999996 1 TTAGGGT 45 1.9195795E-8 40.0 4 GCGATTA 45 1.9195795E-8 40.0 9 ACGGGCC 40 3.44593E-7 39.375 5 TTACGGG 80 0.0 39.375 3 ATAGCGG 35 6.2316267E-6 38.57143 2 TACGGGT 35 6.2316267E-6 38.57143 4 TATACGG 35 6.2316267E-6 38.57143 2 TGGTCAA 100 0.0 38.25 14 TAAGGGA 150 0.0 37.500004 4 AAGGGTC 90 0.0 37.5 5 TCAAGCG 90 0.0 37.5 17 >>END_MODULE