FastQCFastQC Report
Sat 18 Jun 2016
SRR3550293_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550293_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences361348
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174064.816963149097269No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21030.5819874470039962No Hit
GAATCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTC7830.21668862149506846No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGC5340.14777997941042983No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCG4820.13338941961765388No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA4410.12204301670411902No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA4150.11484773680773105No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA4140.11457099527325458No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTCGA207.026859E-445.041
CGGTTAG207.026859E-445.01
TACGGGT207.026859E-445.04
CGCGAAG207.026859E-445.01
TAGCCGT551.8189894E-1245.044
TTAACGG207.026859E-445.02
ACCCCAT207.026859E-445.036
AGTACTA207.026859E-445.031
GCGTAAG253.8854785E-545.01
CGTTATT17600.044.6164781
CGTTTTT118950.044.4325371
CCGATGA2000.042.7518
AATGGGC850.042.352944
GTTATTT18900.042.023812
TAACGGG650.041.538463
ACGGGAT650.041.538465
AAGGGTA1300.039.807695
TATGGGC850.039.7058834
ATGAATG2500.039.621
GTTTTTT134500.039.4795572