FastQCFastQC Report
Sat 18 Jun 2016
SRR3550292_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550292_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences934865
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170201.820583720644157No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13140.14055505340343258No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT207.032611E-445.030
ACGTTCA207.032611E-445.027
TAGAACG253.8902446E-545.01
ATTCGCT207.032611E-445.026
CGCGATT207.032611E-445.025
GTTCGAC207.032611E-445.018
CAGTACG253.8902446E-545.01
GCCCGTC207.032611E-445.011
TAACGCC302.1650649E-644.99999612
CGTTATT3000.042.0000041
CGTTAGG1200.041.2499962
TACGAAT1100.040.90909212
CGAATAT1100.040.90909214
CGTTTTT40650.040.8487051
GACACGA10700.040.1635525
CGAGACA10800.040.00000422
AAACACG10650.039.92957740
GTCAAGC10600.039.90566316
AGACACG10850.039.8156724
AACACGT10850.039.8156741
Produced by FastQC (version 0.11.5)