##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550292_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	934865
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.29042054200339	33.0	31.0	34.0	31.0	34.0
2	32.40470442256368	34.0	31.0	34.0	31.0	34.0
3	32.25606477940665	34.0	31.0	34.0	30.0	34.0
4	35.908529038952146	37.0	35.0	37.0	35.0	37.0
5	33.234171778812986	37.0	35.0	37.0	30.0	37.0
6	34.52196520353206	37.0	35.0	37.0	30.0	37.0
7	35.881374316077725	37.0	35.0	37.0	32.0	37.0
8	35.99224273023378	37.0	35.0	37.0	35.0	37.0
9	37.89728998304568	39.0	38.0	39.0	35.0	39.0
10	37.45531921721318	39.0	37.0	39.0	35.0	39.0
11	37.39097516753756	39.0	37.0	39.0	35.0	39.0
12	37.31408599102544	39.0	37.0	39.0	35.0	39.0
13	37.28457798719601	39.0	37.0	39.0	34.0	39.0
14	38.53736849705572	40.0	38.0	41.0	35.0	41.0
15	38.67195905291138	40.0	38.0	41.0	35.0	41.0
16	38.37196600578693	40.0	37.0	41.0	34.0	41.0
17	38.36941483529708	40.0	37.0	41.0	34.0	41.0
18	38.24231520059046	40.0	37.0	41.0	34.0	41.0
19	38.00280361335594	40.0	37.0	41.0	34.0	41.0
20	38.21825932086451	40.0	36.0	41.0	34.0	41.0
21	38.12081530488359	40.0	36.0	41.0	34.0	41.0
22	38.199909077781285	40.0	36.0	41.0	34.0	41.0
23	38.239889181860484	40.0	36.0	41.0	34.0	41.0
24	38.16227797596444	40.0	36.0	41.0	34.0	41.0
25	37.83210837928471	40.0	35.0	41.0	34.0	41.0
26	37.948302696111206	40.0	35.0	41.0	34.0	41.0
27	37.98270445465388	40.0	35.0	41.0	34.0	41.0
28	37.903714440052845	40.0	35.0	41.0	34.0	41.0
29	37.88793783059586	40.0	35.0	41.0	34.0	41.0
30	37.673942226952555	40.0	35.0	41.0	33.0	41.0
31	37.583561262856136	40.0	35.0	41.0	33.0	41.0
32	37.46610580137239	40.0	35.0	41.0	33.0	41.0
33	37.284813315291515	40.0	35.0	41.0	33.0	41.0
34	37.1505233375942	40.0	35.0	41.0	33.0	41.0
35	37.07605162242677	40.0	35.0	41.0	33.0	41.0
36	36.92849127948955	40.0	35.0	41.0	32.0	41.0
37	36.91002016333909	40.0	35.0	41.0	32.0	41.0
38	36.72628454375766	39.0	35.0	41.0	32.0	41.0
39	36.68368160108679	39.0	35.0	41.0	32.0	41.0
40	36.53707968530215	39.0	35.0	41.0	31.0	41.0
41	36.41235472501377	39.0	35.0	41.0	31.0	41.0
42	36.27102522824151	39.0	35.0	41.0	31.0	41.0
43	36.240632604707635	39.0	35.0	41.0	31.0	41.0
44	36.13051403143769	38.0	35.0	41.0	31.0	41.0
45	36.02425056024132	38.0	35.0	41.0	31.0	41.0
46	35.89298989693699	38.0	35.0	40.0	30.0	41.0
47	35.837022457788024	38.0	35.0	40.0	30.0	41.0
48	35.76177095088595	38.0	35.0	40.0	30.0	41.0
49	35.684979114631524	37.0	35.0	40.0	30.0	41.0
50	35.61941991624459	37.0	35.0	40.0	30.0	41.0
51	34.62546036058682	36.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	9.0
8	32.0
9	62.0
10	47.0
11	37.0
12	29.0
13	25.0
14	30.0
15	27.0
16	67.0
17	131.0
18	230.0
19	388.0
20	727.0
21	1167.0
22	1708.0
23	2545.0
24	3916.0
25	5839.0
26	8417.0
27	10173.0
28	10880.0
29	11509.0
30	13011.0
31	15790.0
32	20175.0
33	31460.0
34	61271.0
35	84328.0
36	75653.0
37	111861.0
38	183326.0
39	279896.0
40	99.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.994191674733784	18.941665374144932	23.50724436148535	15.556898589635937
2	33.81867970241693	23.108791108876684	24.997299075267552	18.07523011343884
3	33.325132505762866	22.157744701106576	28.64552635942088	15.871596433709682
4	27.602060190508794	25.15914062458216	27.974841287244683	19.26395789766437
5	23.21073096115482	35.667074925256586	24.297518893102215	16.82467522048638
6	26.37461023784183	33.23153610414338	25.422173254961944	14.971680403052847
7	82.23433329946035	5.174223016157413	9.41761644729453	3.173827237087708
8	84.43711124066041	4.478507592005262	8.82245030031074	2.261930867023581
9	78.81490910452311	6.833286089435372	10.310151733137939	4.041653072903574
10	41.153749471848876	27.7241098982206	17.48337995325528	13.63876067667524
11	32.536462483888045	25.561016831307192	24.77373738454215	17.128783300262604
12	28.466783974156694	25.04286715194172	27.55520850604098	18.9351403678606
13	24.34950500874458	26.285292528867803	29.190096965872076	20.17510549651554
14	20.733153984799944	27.13461301899205	31.55065169837356	20.581581297834447
15	22.661988629374292	24.60066426703321	32.529723542971446	20.20762356062105
16	26.080664053098573	23.91628737839153	31.0471565413188	18.955892027191094
17	24.275055756713538	23.13221695111059	30.587411016563888	22.005316275611985
18	27.56718884544827	22.484101982639203	28.772175661726557	21.176533510185962
19	26.539874741272808	25.87977943339413	26.532707931091654	21.047637894241415
20	30.52847202537265	24.25098811058281	26.741936001454757	18.478603862589786
21	27.014595690286836	26.02814310087553	27.345124697148787	19.612136511688856
22	26.990635011472246	22.440566284971627	28.438651570012784	22.130147133543346
23	25.963534841929047	26.00567996448685	27.79823824830323	20.23254694528087
24	24.919533836436276	23.881843902595563	30.89900680846967	20.29961545249849
25	24.46000224631364	25.470522481855667	28.287078883047283	21.782396388783408
26	23.613569873725083	26.690912591657618	28.581987773635763	21.113529760981532
27	24.569322843405196	24.884876426008034	27.919539184802083	22.626261545784683
28	21.93033218700026	28.56134308162141	29.294283131789083	20.214041599589244
29	24.0259288774315	26.260369144207985	28.87454338326924	20.83915859509127
30	24.949484684954513	23.544255052868596	30.628272531328054	20.87798773084884
31	27.03919817299824	25.20802468805656	27.46364448342809	20.289132655517108
32	25.866301551560923	24.734801281468446	28.80105683708343	20.597840329887205
33	24.350253779957534	25.991881180705235	27.398822289849335	22.259042749487897
34	22.443240467875043	27.678862723494834	30.21773197199596	19.660164836634166
35	22.986955335797145	28.303872751680725	28.808651516529128	19.900520395993006
36	22.98631353190033	31.79945767570719	25.384306825049606	19.829921967342877
37	24.745498013082106	28.451594615265307	25.393933883501894	21.408973488150696
38	23.865905772491217	28.84855032544806	26.387018446513665	20.898525455547055
39	25.560909863991053	25.892508544014376	26.216512544592003	22.330069047402564
40	25.668091114759882	25.64455830520984	26.849545121488127	21.837805458542142
41	21.95718098335054	27.78358372599252	27.036844892043238	23.222390398613705
42	24.10166173725618	27.09075641937606	25.42099661448444	23.386585228883312
43	23.93907141672862	25.784043685451913	26.767501190011394	23.50938370780808
44	23.93629026650907	27.127232274178624	26.819273371021485	22.117204088290823
45	24.41432720232333	26.103662026067937	26.580843223353106	22.90116754825563
46	23.414503698394956	27.406416969295034	26.14944403737438	23.029635294935634
47	22.880950725505823	27.959758895669427	27.776096013862965	21.383194364961785
48	23.85114428286437	26.536237852524163	28.1735865606264	21.439031303985068
49	23.958753402897745	27.6123290528579	27.597246661282643	20.83167088296171
50	22.291132944328858	28.071111871767577	27.774705438753188	21.86304974515037
51	22.391896156129494	26.10216448364202	27.292389810293464	24.213549549935017
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	419.0
1	1497.0
2	2575.0
3	13388.0
4	24201.0
5	14314.0
6	4427.0
7	4017.0
8	3607.0
9	3478.5
10	3350.0
11	3231.5
12	3113.0
13	3032.5
14	2952.0
15	3005.0
16	3058.0
17	2960.5
18	2863.0
19	2791.5
20	2720.0
21	2994.5
22	3269.0
23	3801.0
24	4333.0
25	4992.0
26	6917.0
27	8183.0
28	10617.5
29	13052.0
30	14341.0
31	15630.0
32	18047.0
33	20464.0
34	23098.5
35	25733.0
36	26821.5
37	27910.0
38	30234.0
39	32558.0
40	36198.5
41	39839.0
42	42151.0
43	44463.0
44	45942.5
45	47422.0
46	49260.5
47	51099.0
48	51720.5
49	52342.0
50	54338.0
51	56334.0
52	59746.0
53	63158.0
54	62316.0
55	61474.0
56	57387.0
57	53300.0
58	51653.5
59	50007.0
60	49590.0
61	49173.0
62	47099.0
63	45025.0
64	39912.0
65	34799.0
66	29664.5
67	24530.0
68	20636.5
69	16743.0
70	14163.0
71	11583.0
72	10216.5
73	8850.0
74	7685.0
75	5322.5
76	4125.0
77	3171.5
78	2218.0
79	1551.0
80	884.0
81	679.0
82	474.0
83	348.0
84	222.0
85	171.0
86	120.0
87	84.5
88	49.0
89	37.0
90	25.0
91	17.0
92	9.0
93	8.0
94	7.0
95	4.5
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	934865.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.55105998894051
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.98355078758274	13.724732888552744
2	5.979430270318631	2.2184953928873536
3	2.511338629275873	1.3976398069272105
4	1.6426215564162947	1.2188948412882201
5	1.2340475886313373	1.144644542295366
6	0.8839677471075389	0.9839123222928329
7	0.7042956374344266	0.91458014339966
8	0.5674604505760298	0.8421594287989711
9	0.5018811390652581	0.8379384406305658
>10	10.005086852231404	49.34191970279688
>50	1.8703817026152185	21.344268530234945
>100	0.10943093452998585	3.4084155899544353
>500	0.005323667085242555	0.6105542108272908
>1k	5.915185650269505E-4	0.14418911449084473
>5k	0.0	0.0
>10k+	5.915185650269505E-4	1.8676550446226614
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17020	1.820583720644157	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1314	0.14055505340343258	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.278692645462179E-4	0.0	0.0	0.002246313638867644	0.0
2	4.278692645462179E-4	0.0	0.0	0.009947960400699566	0.0
3	4.278692645462179E-4	0.0	0.0	0.014333620362298299	0.0
4	4.278692645462179E-4	0.0	0.0	0.01882624764003359	0.0
5	4.278692645462179E-4	0.0	0.0	0.0382942991768865	0.0
6	4.278692645462179E-4	0.0	0.0	0.04695865178394742	0.0
7	4.278692645462179E-4	0.0	0.0	0.05808325266214908	0.0
8	4.278692645462179E-4	0.0	0.0	0.0740213827664957	0.0
9	4.278692645462179E-4	0.0	0.0	0.08076032368309863	0.0
10	4.278692645462179E-4	0.0	0.0	0.09840993084563011	0.0
11	4.278692645462179E-4	0.0	0.0	0.11220871462724565	0.0
12	4.278692645462179E-4	0.0	0.0	0.1327464393254641	0.0
13	4.278692645462179E-4	0.0	0.0	0.1395923475582036	0.0
14	4.278692645462179E-4	0.0	0.0	0.14355013825525612	0.0
15	4.278692645462179E-4	0.0	0.0	0.14857760211367416	0.0
16	4.278692645462179E-4	0.0	0.0	0.1598091703080124	0.0
17	4.278692645462179E-4	0.0	0.0	0.17596123504463212	0.0
18	4.278692645462179E-4	0.0	0.0	0.1917923978328422	0.0
19	4.278692645462179E-4	0.0	0.0	0.20099158702058587	0.0
20	4.278692645462179E-4	0.0	0.0	0.21350676300856272	0.0
21	4.278692645462179E-4	0.0	0.0	0.22591497168040306	0.0
22	4.278692645462179E-4	0.0	0.0	0.23864408230065304	0.0
23	4.278692645462179E-4	0.0	0.0	0.2549031143534093	0.0
24	4.278692645462179E-4	0.0	0.0	0.2683809961866152	0.0
25	0.0012836077936386537	0.0	0.0	0.2815379760714114	0.0
26	0.0012836077936386537	0.0	0.0	0.2958715964337097	0.0
27	0.0012836077936386537	0.0	0.0	0.3087076743700962	0.0
28	0.0013905751097752083	0.0	0.0	0.3255015430035353	0.0
29	0.0013905751097752083	0.0	0.0	0.34176057505629154	0.0
30	0.0013905751097752083	0.0	0.0	0.3656142865547432	0.0
31	0.0013905751097752083	0.0	0.0	0.38636594588523476	0.0
32	0.0013905751097752083	0.0	0.0	0.40904301690618433	0.0
33	0.0013905751097752083	0.0	0.0	0.43621271520486915	0.0
34	0.0013905751097752083	0.0	0.0	0.45878281890968214	0.0
35	0.0013905751097752083	0.0	0.0	0.48456194209859177	0.0
36	0.0013905751097752083	0.0	0.0	0.5115177057650035	0.0
37	0.0013905751097752083	0.0	0.0	0.541896423547785	0.0
38	0.0013905751097752083	0.0	0.0	0.5762329320276189	0.0
39	0.0013905751097752083	0.0	0.0	0.6201964989597428	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTAT	20	7.032611E-4	45.0	30
ACGTTCA	20	7.032611E-4	45.0	27
TAGAACG	25	3.8902446E-5	45.0	1
ATTCGCT	20	7.032611E-4	45.0	26
CGCGATT	20	7.032611E-4	45.0	25
GTTCGAC	20	7.032611E-4	45.0	18
CAGTACG	25	3.8902446E-5	45.0	1
GCCCGTC	20	7.032611E-4	45.0	11
TAACGCC	30	2.1650649E-6	44.999996	12
CGTTATT	300	0.0	42.000004	1
CGTTAGG	120	0.0	41.249996	2
TACGAAT	110	0.0	40.909092	12
CGAATAT	110	0.0	40.909092	14
CGTTTTT	4065	0.0	40.848705	1
GACACGA	1070	0.0	40.16355	25
CGAGACA	1080	0.0	40.000004	22
AAACACG	1065	0.0	39.929577	40
GTCAAGC	1060	0.0	39.905663	16
AGACACG	1085	0.0	39.81567	24
AACACGT	1085	0.0	39.81567	41
>>END_MODULE