FastQCFastQC Report
Sat 18 Jun 2016
SRR3550290_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550290_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences263491
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58642.225502958355314No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4560.17306093946282797No Hit
GCTTTTGGGATAAATAAACCTGTTAAAATTTTCTGGACAATGCCAGCATTT2940.11157876360103382No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGCC207.0233777E-445.00000441
TACCCCT207.0233777E-445.00000415
ACGCCTC207.0233777E-445.00000436
CGAAATC207.0233777E-445.00000412
ATTACGG207.0233777E-445.0000042
GAATCGG207.0233777E-445.0000042
TGACGTC207.0233777E-445.00000426
ACCTATT207.0233777E-445.00000432
CTAACGA302.1591113E-645.00000443
CTCGGAA207.0233777E-445.00000429
CTACGAC207.0233777E-445.00000413
TACGACT207.0233777E-445.00000414
ATGACGT207.0233777E-445.00000425
CGCAGAA207.0233777E-445.00000419
GCCTAAC207.0233777E-445.00000441
CGTAAGG207.0233777E-445.0000042
TACTACG207.0233777E-445.0000041
CCAACGG302.1591113E-645.0000042
CCTATTC207.0233777E-445.00000433
CGACTCA207.0233777E-445.00000416