##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550290_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	263491
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.30963486418891	33.0	31.0	34.0	31.0	34.0
2	32.425407319415086	34.0	31.0	34.0	31.0	34.0
3	32.25269553798802	34.0	31.0	34.0	30.0	34.0
4	35.91290784125454	37.0	35.0	37.0	35.0	37.0
5	33.23172707986231	37.0	35.0	37.0	30.0	37.0
6	34.528321650454856	37.0	35.0	37.0	30.0	37.0
7	35.87791613375789	37.0	35.0	37.0	32.0	37.0
8	36.039519376373384	37.0	35.0	37.0	35.0	37.0
9	37.96927409285327	39.0	38.0	39.0	35.0	39.0
10	37.45354490286196	39.0	37.0	39.0	34.0	39.0
11	37.45914281702221	39.0	37.0	39.0	35.0	39.0
12	37.25239571750079	39.0	37.0	39.0	35.0	39.0
13	37.108781704118925	39.0	37.0	39.0	33.0	39.0
14	38.31694061656755	40.0	37.0	41.0	33.0	41.0
15	38.47338239256749	40.0	37.0	41.0	34.0	41.0
16	38.24740882990311	40.0	36.0	41.0	34.0	41.0
17	38.27089729819994	40.0	37.0	41.0	34.0	41.0
18	38.13308613956454	40.0	36.0	41.0	34.0	41.0
19	37.89216329969525	39.0	36.0	41.0	34.0	41.0
20	38.06843877020467	40.0	35.0	41.0	34.0	41.0
21	37.94748966757878	40.0	35.0	41.0	34.0	41.0
22	38.049049113631966	40.0	35.0	41.0	34.0	41.0
23	38.11124478634944	40.0	35.0	41.0	34.0	41.0
24	38.03218705762246	40.0	35.0	41.0	34.0	41.0
25	37.72347063087544	40.0	35.0	41.0	34.0	41.0
26	37.82728442337689	40.0	35.0	41.0	34.0	41.0
27	37.880345059224034	40.0	35.0	41.0	34.0	41.0
28	37.8131549085168	40.0	35.0	41.0	34.0	41.0
29	37.804296920957455	40.0	35.0	41.0	34.0	41.0
30	37.610779874834435	40.0	35.0	41.0	34.0	41.0
31	37.5168525680194	40.0	35.0	41.0	34.0	41.0
32	37.37222144209859	40.0	35.0	41.0	33.0	41.0
33	37.20028008546782	40.0	35.0	41.0	33.0	41.0
34	37.07933477803796	40.0	35.0	41.0	33.0	41.0
35	36.97705044954097	40.0	35.0	41.0	33.0	41.0
36	36.820320238641926	39.0	35.0	41.0	33.0	41.0
37	36.78182177000353	39.0	35.0	41.0	33.0	41.0
38	36.64771092750796	39.0	35.0	41.0	32.0	41.0
39	36.59479830430641	39.0	35.0	41.0	32.0	41.0
40	36.45365875874318	39.0	35.0	41.0	31.0	41.0
41	36.36258923454691	39.0	35.0	41.0	31.0	41.0
42	36.212178025055884	39.0	35.0	41.0	31.0	41.0
43	36.22882375489106	39.0	35.0	41.0	31.0	41.0
44	36.125324963661	38.0	35.0	41.0	31.0	41.0
45	36.03796714119268	38.0	35.0	41.0	31.0	41.0
46	35.960131465590855	38.0	35.0	41.0	31.0	41.0
47	35.91268392468813	38.0	35.0	41.0	31.0	41.0
48	35.859813048643026	38.0	35.0	40.0	31.0	41.0
49	35.816703416814995	37.0	35.0	40.0	31.0	41.0
50	35.73416549331856	37.0	35.0	40.0	31.0	41.0
51	34.778721094838154	36.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	7.0
9	23.0
10	19.0
11	14.0
12	3.0
13	8.0
14	4.0
15	9.0
16	15.0
17	27.0
18	56.0
19	114.0
20	160.0
21	289.0
22	529.0
23	730.0
24	1137.0
25	1695.0
26	2412.0
27	2836.0
28	2893.0
29	3036.0
30	3542.0
31	4288.0
32	5861.0
33	9158.0
34	18687.0
35	29341.0
36	19757.0
37	28604.0
38	48125.0
39	80072.0
40	38.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.88864894816142	18.68109347188329	25.01489614446034	16.41536143549495
2	33.367743110770384	23.208382829015033	26.64113764796521	16.782736412249374
3	31.722146107457185	22.499440208583973	30.10766971167896	15.670743972279888
4	26.26541323992091	24.65966579503664	30.36005024839558	18.71487071664687
5	23.343871327673433	34.57841064780201	26.45061880671446	15.627099217810095
6	25.20427642689883	32.95065106588081	28.139481044893373	13.705591462326986
7	81.43617808577902	4.862784687143015	10.644006816172089	3.0570304109058757
8	86.04696175580949	3.2175672034338936	8.772595648428219	1.9628753923283906
9	81.53447366323707	4.4274757012573485	10.075486449252537	3.962564186253041
10	41.65037895032468	24.40728525832002	18.914877547999744	15.027458243355559
11	35.10556337787629	24.6095692072974	24.165151750913694	16.119715663912622
12	30.63444292214914	24.00575351719793	27.15956142714552	18.200242133507405
13	24.162495113685097	28.705724294188418	29.121677780265742	18.010102811860744
14	20.826517793776638	30.581689697181307	30.031386271257844	18.560406237784214
15	19.59801283535301	25.6395854127845	36.07333836829341	18.689063383569078
16	24.034217487504318	22.138896584703083	35.29266654269026	18.53421938510234
17	23.65052316777423	21.401869513569725	31.15817997578665	23.789427342869395
18	25.574687560485938	21.89790163610901	32.13354535828548	20.39386544511957
19	26.43505850294697	25.113950761126564	28.10152908448486	20.349461651441604
20	28.942165007533465	25.30902383762633	27.917841596107646	17.830969558732555
21	25.74281474509566	26.23467215199001	29.269690425858947	18.752822677055384
22	25.012619026835832	22.905526184955082	29.505751619599913	22.576103168609173
23	23.409148699576075	26.434299463738796	28.651073471200156	21.50547836548497
24	21.961281409991233	24.94772117453727	33.27362224895727	19.817375166514225
25	22.06640834032282	26.037321957865732	31.630681882872658	20.265587818938787
26	20.841698577940043	27.800570038445336	30.540322060336027	20.817409323278593
27	21.28459795590741	27.732636029314094	29.812782979304796	21.169983035473695
28	20.549848002398562	28.406283326565234	32.21400351435154	18.82986515668467
29	21.3001582596749	25.469560630154355	32.219316788808726	21.010964321362017
30	22.864917587317972	25.964454193881387	32.836036145447096	18.334592073353548
31	25.387204876067877	26.087418545604972	28.739122019347906	19.786254558979245
32	25.087004869236516	25.58531410940032	29.567233795461707	19.760447225901455
33	24.876751008573347	25.69727239260544	27.61308735402727	21.81288924479394
34	21.432231081896536	28.690163990420924	29.699306617683334	20.178298309999203
35	21.45841793457841	27.753509607538778	30.796877312697585	19.991195145185227
36	24.2850799458046	29.390377659958023	27.35197786641669	18.972564527820683
37	23.13741266305111	28.266999631865986	28.556193570178866	20.039394134904036
38	22.686543373397953	29.027556918452625	28.222216318583936	20.063683389565487
39	22.400765111521835	27.777419342596144	28.929640860598653	20.89217468528337
40	24.797051891715466	26.193684034748816	28.116709868648265	20.892554204887453
41	21.409839425255512	26.355738905693173	29.031352114493476	23.20306955455784
42	21.945721106223743	27.930745262646546	27.822202655878193	22.301330975251528
43	22.551434394343637	26.476805659396334	27.915564478483134	23.056195467776888
44	22.83531505819933	26.997127036597075	28.41653035587553	21.75102754932806
45	21.1392419475428	29.57368562873115	27.483291649430154	21.8037807742959
46	23.29073858310151	27.973251458304077	27.430538424462313	21.305471534132096
47	21.124820202587564	28.39413869923451	29.412769316599046	21.068271781578876
48	22.367746905966428	26.843042077338502	29.5698904326903	21.219320584004766
49	22.726392931826894	26.577378354478903	30.035940506506865	20.660288207187342
50	21.896004038088588	27.799810999237167	29.568372354273958	20.735812608400288
51	21.167326398245102	27.22597735786042	28.494711394317072	23.111984849577404
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	178.0
1	428.5
2	679.0
3	4250.5
4	7822.0
5	4532.0
6	1242.0
7	1165.0
8	1088.0
9	1051.5
10	1015.0
11	1021.0
12	1027.0
13	999.0
14	971.0
15	948.5
16	926.0
17	963.5
18	1001.0
19	977.5
20	954.0
21	963.0
22	972.0
23	1102.0
24	1232.0
25	1616.0
26	2076.0
27	2152.0
28	2686.0
29	3220.0
30	3816.0
31	4412.0
32	5089.5
33	5767.0
34	6416.5
35	7066.0
36	7451.0
37	7836.0
38	9240.5
39	10645.0
40	11289.5
41	11934.0
42	14039.0
43	16144.0
44	16864.0
45	17584.0
46	18378.5
47	19173.0
48	18576.5
49	17980.0
50	18297.5
51	18615.0
52	17754.0
53	16893.0
54	16193.0
55	15493.0
56	14799.0
57	14105.0
58	13399.5
59	12694.0
60	11723.0
61	10752.0
62	9493.0
63	8234.0
64	7360.5
65	6487.0
66	5398.0
67	4309.0
68	3870.5
69	3432.0
70	2951.0
71	2470.0
72	2347.5
73	2225.0
74	1692.5
75	939.0
76	718.0
77	649.0
78	580.0
79	388.0
80	196.0
81	120.5
82	45.0
83	29.5
84	14.0
85	28.5
86	43.0
87	24.0
88	5.0
89	3.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	263491.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.05312515417984
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.32539409375646	16.3910721808335
2	5.911406346802506	2.607299680064974
3	2.708749225579955	1.7920915704900737
4	1.860329042472637	1.6410427680641844
5	1.233909272389344	1.3605777806452592
6	0.9413505885592345	1.245583340607459
7	0.6522337715977146	1.0068655096379002
8	0.5610242995800923	0.9897871274540686
9	0.4973497625111861	0.9871304902254727
>10	10.602670888690026	59.58268024334797
>50	0.6608384387691884	8.593082875695943
>100	0.04302333585736903	1.5772834745778792
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0017209334342947616	2.225502958355314
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5864	2.225502958355314	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	456	0.17306093946282797	No Hit
GCTTTTGGGATAAATAAACCTGTTAAAATTTTCTGGACAATGCCAGCATTT	294	0.11157876360103382	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.005692794061277235	0.0
2	0.0	0.0	0.0	0.04516283288613274	0.0
3	0.0	0.0	0.0	0.052753224967835716	0.0
4	0.0	0.0	0.0	0.07476536200477436	0.0
5	0.0	0.0	0.0	0.15977775331984773	0.0
6	0.0	0.0	0.0	0.17951277273227548	0.0
7	0.0	0.0	0.0	0.2151876155162795	0.0
8	0.0	0.0	0.0	0.2626275660269231	0.0
9	0.0	0.0	0.0	0.2766697913780736	0.0
10	0.0	0.0	0.0	0.3780015256688084	0.0
11	0.0	0.0	0.0	0.40912213320379065	0.0
12	0.0	0.0	0.0	0.5389178378009116	0.0
13	0.0	0.0	0.0	0.5601709356296799	0.0
14	0.0	0.0	0.0	0.5696589257318087	0.0
15	0.0	0.0	0.0	0.5840806706870444	0.0
16	0.0	0.0	0.0	0.6125446409934305	0.0
17	0.0	0.0	0.0	0.6372134152589652	0.0
18	0.0	0.0	0.0	0.6766834540838207	0.0
19	0.0	0.0	0.0	0.6956594342880782	0.0
20	0.0	0.0	0.0	0.7210872477617831	0.0
21	0.0	0.0	0.0	0.7442379436109772	0.0
22	0.0	0.0	0.0	0.7780151883745555	0.0
23	0.0	0.0	0.0	0.8091357959095377	0.0
24	0.0	0.0	0.0	0.8364612074036685	0.0
25	0.0	0.0	0.0	0.8588528640446922	0.0
26	0.0	0.0	0.0	0.8774493246448646	0.0
27	0.0	0.0	0.0	0.9081904125757616	0.0
28	0.0	0.0	0.0	0.9366543828821478	0.0
29	0.0	0.0	0.0	0.9673954708130449	0.0
30	0.0	0.0	0.0	1.0106607056787518	0.0
31	0.0	0.0	0.0	1.0470945876709261	0.0
32	0.0	0.0	0.0	1.0801127932263341	0.0
33	0.0	0.0	0.0	1.118064753634849	0.0
34	0.0	0.0	0.0	1.15867335127196	0.0
35	0.0	0.0	0.0	1.2011795469294966	0.0
36	0.0	0.0	0.0	1.2440652621911186	0.0
37	0.0	0.0	0.0	1.2892280950772512	0.0
38	0.0	0.0	0.0	1.3404632416287463	0.0
39	0.0	0.0	0.0	1.395493584221093	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGGCC	20	7.0233777E-4	45.000004	41
TACCCCT	20	7.0233777E-4	45.000004	15
ACGCCTC	20	7.0233777E-4	45.000004	36
CGAAATC	20	7.0233777E-4	45.000004	12
ATTACGG	20	7.0233777E-4	45.000004	2
GAATCGG	20	7.0233777E-4	45.000004	2
TGACGTC	20	7.0233777E-4	45.000004	26
ACCTATT	20	7.0233777E-4	45.000004	32
CTAACGA	30	2.1591113E-6	45.000004	43
CTCGGAA	20	7.0233777E-4	45.000004	29
CTACGAC	20	7.0233777E-4	45.000004	13
TACGACT	20	7.0233777E-4	45.000004	14
ATGACGT	20	7.0233777E-4	45.000004	25
CGCAGAA	20	7.0233777E-4	45.000004	19
GCCTAAC	20	7.0233777E-4	45.000004	41
CGTAAGG	20	7.0233777E-4	45.000004	2
TACTACG	20	7.0233777E-4	45.000004	1
CCAACGG	30	2.1591113E-6	45.000004	2
CCTATTC	20	7.0233777E-4	45.000004	33
CGACTCA	20	7.0233777E-4	45.000004	16
>>END_MODULE