##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550289_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	932519
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.23590082346848	33.0	31.0	34.0	30.0	34.0
2	32.325674865605954	34.0	31.0	34.0	30.0	34.0
3	32.202157811261756	34.0	31.0	34.0	30.0	34.0
4	35.83969120200232	37.0	35.0	37.0	35.0	37.0
5	33.22346568809858	37.0	35.0	37.0	30.0	37.0
6	34.462802366493335	37.0	35.0	37.0	29.0	37.0
7	35.77383302645844	37.0	35.0	37.0	32.0	37.0
8	35.87023749650141	37.0	35.0	37.0	35.0	37.0
9	37.72599914854282	39.0	38.0	39.0	35.0	39.0
10	37.2933731108964	39.0	37.0	39.0	34.0	39.0
11	37.23911684373187	39.0	37.0	39.0	34.0	39.0
12	37.27831711739922	39.0	37.0	39.0	34.0	39.0
13	37.28047685891655	39.0	37.0	39.0	34.0	39.0
14	38.57996673526223	40.0	38.0	41.0	34.0	41.0
15	38.64472680985588	40.0	38.0	41.0	35.0	41.0
16	38.38720068974466	40.0	38.0	41.0	34.0	41.0
17	38.365369499173745	40.0	38.0	41.0	34.0	41.0
18	38.2335030170967	40.0	37.0	41.0	34.0	41.0
19	38.01804896200506	40.0	37.0	41.0	34.0	41.0
20	38.19251511229262	40.0	37.0	41.0	34.0	41.0
21	38.19233924456231	40.0	37.0	41.0	34.0	41.0
22	38.28072993686992	40.0	37.0	41.0	34.0	41.0
23	38.33470309988322	40.0	37.0	41.0	34.0	41.0
24	38.23006287271359	40.0	37.0	41.0	34.0	41.0
25	37.935765383868855	40.0	36.0	41.0	34.0	41.0
26	37.99605477207435	40.0	36.0	41.0	34.0	41.0
27	38.05278498346951	40.0	36.0	41.0	34.0	41.0
28	37.96244151593694	40.0	36.0	41.0	34.0	41.0
29	37.93188557015996	40.0	36.0	41.0	34.0	41.0
30	37.75649611428829	40.0	36.0	41.0	33.0	41.0
31	37.69826352063604	40.0	36.0	41.0	33.0	41.0
32	37.607894316362454	40.0	36.0	41.0	33.0	41.0
33	37.433192245948874	40.0	36.0	41.0	33.0	41.0
34	37.34142253401807	40.0	36.0	41.0	33.0	41.0
35	37.27411666679178	40.0	35.0	41.0	33.0	41.0
36	37.1068160541501	40.0	35.0	41.0	32.0	41.0
37	37.07964234508895	40.0	35.0	41.0	32.0	41.0
38	36.90884475276107	40.0	35.0	41.0	32.0	41.0
39	36.87291518993179	40.0	35.0	41.0	31.0	41.0
40	36.71820949492718	39.0	35.0	41.0	31.0	41.0
41	36.58039353621749	39.0	35.0	41.0	31.0	41.0
42	36.498455259356646	39.0	35.0	41.0	31.0	41.0
43	36.500520632823566	39.0	35.0	41.0	31.0	41.0
44	36.445047232281595	39.0	35.0	41.0	31.0	41.0
45	36.414606029475	39.0	35.0	41.0	31.0	41.0
46	36.31369548502497	39.0	35.0	41.0	31.0	41.0
47	36.205874625610846	39.0	35.0	41.0	30.0	41.0
48	36.14413647335872	39.0	35.0	41.0	30.0	41.0
49	36.090864636538235	39.0	35.0	41.0	30.0	41.0
50	35.98893212899684	38.0	35.0	41.0	30.0	41.0
51	34.939871466425885	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	7.0
8	20.0
9	60.0
10	67.0
11	43.0
12	18.0
13	22.0
14	19.0
15	37.0
16	72.0
17	170.0
18	322.0
19	632.0
20	1084.0
21	1609.0
22	2061.0
23	3021.0
24	4228.0
25	6049.0
26	8185.0
27	9624.0
28	10356.0
29	11055.0
30	12854.0
31	16064.0
32	21056.0
33	30276.0
34	52773.0
35	69820.0
36	72369.0
37	108428.0
38	182268.0
39	307733.0
40	117.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.589889321289974	15.628421512054983	20.193154241361302	20.588534925293747
2	30.257399581134543	26.465948683083134	25.655884759452626	17.620766976329705
3	32.000313130349085	24.965818390831714	27.29263425195626	15.741234226862938
4	28.02259256915945	24.964746026622514	28.759521253722443	18.253140150495593
5	24.78566120368593	34.64251130539967	22.983874859386244	17.58795263152815
6	25.46564734874035	36.41759578089025	24.588560662034766	13.528196208334627
7	80.74891771642186	5.144667293642274	10.655332491884884	3.4510824980509778
8	82.7119876377854	5.865296042225413	8.628135190811125	2.794581129178065
9	76.83124955094749	7.071384068313889	9.91036107575288	6.187005304985743
10	37.49510733829552	32.10851467905748	16.73810399573628	13.658273986910721
11	28.611963938536373	24.84335439814095	29.22439113841112	17.32029052491156
12	25.525485271613768	23.323492604440233	31.63034747817471	19.520674645771294
13	24.64936371269647	23.469012427628822	32.97938165334969	18.902242206325017
14	21.727064006202554	27.61520140608395	30.448602119635098	20.209132468078398
15	21.076460640480246	26.08000480419166	33.38623663432059	19.45729792100751
16	25.19476814949615	24.641642690390224	29.96196324149964	20.201625918613992
17	25.12098949190311	23.573889647288688	30.30898029959711	20.996140561211085
18	24.779441491272564	24.20347467451065	29.971614519382445	21.045469314834335
19	24.321970919627375	25.695991180876742	28.68563535970849	21.296402539787394
20	26.290080952774154	25.93845272857711	28.971098712197822	18.800367606450912
21	25.444414537398163	26.361178699844185	29.382779332110122	18.811627430647526
22	24.85300567602376	23.504614919374298	29.795210606968865	21.847168797633078
23	23.689597745461484	26.506698523032775	29.692156406464644	20.111547325041098
24	23.103014523028484	25.85727475794059	30.120136962356796	20.919573756674126
25	23.0742751622219	27.98237891131441	28.205537903249155	20.73780802321454
26	22.63996765749545	26.722458201924038	28.478990776595438	22.158583363985077
27	23.580431068964813	26.54348061540837	28.8717977864258	21.004290529201015
28	21.934673717103887	27.094997528200498	30.258686418185583	20.71164233651003
29	23.012721456613754	25.687197794361293	29.99670784187775	21.3033729071472
30	23.699141786923374	25.84762348005778	29.964965861285396	20.488268871733446
31	24.125513796501732	26.176839292282516	28.14698681742678	21.550660093788974
32	24.1525373745736	26.048477296441146	28.808635534503857	20.9903497944814
33	23.446385542814678	25.959471067077455	28.666761749626552	21.927381640481318
34	23.094650082196715	27.564585815409657	28.551268124295593	20.78949597809803
35	24.069750857623276	26.1810215126984	28.890456923665898	20.858770706012425
36	24.019456976211746	28.526710983904884	26.955268471741594	20.498563568141776
37	23.669759007591264	27.71300102196309	27.903452905517206	20.713787064928436
38	22.93207966808183	28.02945570009834	27.898627266575804	21.139837365244034
39	23.89677851067914	26.824761747481823	27.592360048427967	21.686099693411073
40	23.075133053589255	26.66658802662466	29.542668835702006	20.715610084084076
41	22.723612065813136	27.364589890393653	28.29411518692917	21.617682856864043
42	22.614767098579225	27.122771761218807	28.679951829399723	21.582509310802248
43	23.230411391081578	26.64664205233352	27.46893092794892	22.654015628635985
44	22.83674648988385	27.02936883859739	28.01036761717456	22.1235170543442
45	22.382064065182586	26.51259652618338	28.303766464811975	22.80157294382206
46	24.01613264716322	27.41027260570562	27.8346071232865	20.73898762384466
47	22.574446204313265	27.612306022719107	29.265140978360765	20.548106794606866
48	22.963178230148664	27.3296308171737	27.924685716859386	21.78250523581825
49	22.222603507274382	26.67602483166563	29.412698293546836	21.688673367513157
50	22.34882077469735	27.316976919505127	28.73464240406898	21.599559901728544
51	22.546350262032195	26.638813793606346	28.185591928958015	22.62924401540344
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	257.0
1	1254.0
2	2251.0
3	9898.5
4	17546.0
5	10642.5
6	3739.0
7	3389.5
8	3040.0
9	3031.5
10	3023.0
11	3048.0
12	3073.0
13	3122.0
14	3171.0
15	3241.5
16	3312.0
17	3195.5
18	3079.0
19	3269.0
20	3459.0
21	3623.0
22	3787.0
23	4478.5
24	5170.0
25	5778.0
26	7775.5
27	9165.0
28	11109.0
29	13053.0
30	15984.5
31	18916.0
32	21595.0
33	24274.0
34	27135.5
35	29997.0
36	32438.5
37	34880.0
38	36506.0
39	38132.0
40	41787.5
41	45443.0
42	48114.5
43	50786.0
44	54069.5
45	57353.0
46	65506.0
47	73659.0
48	67178.5
49	60698.0
50	59221.0
51	57744.0
52	55678.5
53	53613.0
54	52791.5
55	51970.0
56	49476.5
57	46983.0
58	44545.0
59	42107.0
60	40424.5
61	38742.0
62	36310.5
63	33879.0
64	29663.0
65	25447.0
66	22323.5
67	19200.0
68	16477.5
69	13755.0
70	12254.5
71	10754.0
72	9437.5
73	8121.0
74	6929.5
75	4499.5
76	3261.0
77	2528.0
78	1795.0
79	1421.5
80	1048.0
81	754.0
82	460.0
83	290.0
84	120.0
85	94.5
86	69.0
87	48.0
88	27.0
89	21.5
90	16.0
91	15.5
92	15.0
93	10.5
94	6.0
95	3.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	932519.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.882566727544088
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.75344603709235	17.854099089491843
2	6.068124804111728	3.0198104069875096
3	2.8686288399803646	2.141365455821064
4	2.0218942939922298	2.012396787452092
5	1.5349174811010518	1.9096343322385405
6	1.3351991248148805	1.9933908790658836
7	1.1214692369545616	1.953352318498688
8	0.9996592047038445	1.989926949267765
9	0.8961204702643398	2.0067999657543565
>10	11.219703229600356	57.86695189750417
>50	0.14512191515491155	2.308118869053294
>100	0.02983815072329464	1.342821663768732
>500	0.003164652349440341	0.5765235006127797
>1k	0.0018083727711087662	1.0689026932336845
>5k	4.5209319277719155E-4	0.5677490941644291
>10k+	4.5209319277719155E-4	1.38815609708521
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12340	1.3232974341541566	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTC	5047	0.5412222163837949	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGC	3617	0.38787413446803765	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCG	3555	0.38122547637099086	No Hit
GAACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT	1228	0.131686324889895	RNA PCR Primer, Index 27 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	1102	0.11817453585396115	RNA PCR Primer, Index 27 (95% over 23bp)
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	978	0.10487721965986752	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0723642092010994E-4	0.0	0.0	0.010509169250170773	0.0
2	1.0723642092010994E-4	0.0	0.0	0.14616324171410985	0.0
3	2.1447284184021988E-4	0.0	0.0	0.1663236888470905	0.0
4	2.1447284184021988E-4	0.0	0.0	0.32310333623229126	0.0
5	2.1447284184021988E-4	0.0	0.0	0.9305976607447141	0.0
6	2.1447284184021988E-4	0.0	0.0	1.0256091296799315	0.0
7	2.1447284184021988E-4	0.0	0.0	1.153113234153942	0.0
8	2.1447284184021988E-4	0.0	0.0	1.274933808319187	0.0
9	3.217092627603298E-4	0.0	0.0	1.298204111658851	0.0
10	3.217092627603298E-4	0.0	0.0	1.7561036289877203	0.0
11	3.217092627603298E-4	0.0	0.0	1.7912771750495164	0.0
12	3.217092627603298E-4	0.0	0.0	2.273519359927251	0.0
13	3.217092627603298E-4	0.0	0.0	2.313840254193212	0.0
14	3.217092627603298E-4	0.0	0.0	2.3426868514207215	0.0
15	4.2894568368043976E-4	0.0	0.0	2.4251516591082862	0.0
16	4.2894568368043976E-4	0.0	0.0	2.468904118843691	0.0
17	4.2894568368043976E-4	0.0	0.0	2.5036487192218067	0.0
18	4.2894568368043976E-4	0.0	0.0	2.5315301886610353	0.0
19	4.2894568368043976E-4	0.0	0.0	2.6050943734122307	0.0
20	4.2894568368043976E-4	0.0	0.0	2.6313672965376576	0.0
21	4.2894568368043976E-4	0.0	0.0	2.6504553794614374	0.0
22	4.2894568368043976E-4	0.0	0.0	2.6876664175207154	0.0
23	4.2894568368043976E-4	0.0	0.0	2.7119018486486604	0.0
24	4.2894568368043976E-4	0.0	0.0	2.733778078516363	0.0
25	5.361821046005496E-4	0.0	0.0	2.751579324389101	0.0
26	5.361821046005496E-4	0.0	0.0	2.7735627906777234	0.0
27	5.361821046005496E-4	0.0	0.0	2.8048758255863957	0.0
28	5.361821046005496E-4	0.0	0.0	2.8297546752398612	0.0
29	5.361821046005496E-4	0.0	0.0	2.8570999625744893	0.0
30	5.361821046005496E-4	0.0	0.0	2.895168892001128	0.0
31	7.506549464407695E-4	0.0	0.0	2.924015489228638	0.0
32	7.506549464407695E-4	0.0	0.0	2.9569370704511115	0.0
33	7.506549464407695E-4	0.0	0.0	2.989751415252665	0.0
34	7.506549464407695E-4	0.0	0.0	3.02385259710526	0.0
35	7.506549464407695E-4	0.0	0.0	3.061063635164538	0.0
36	7.506549464407695E-4	0.0	0.0	3.0963444176472543	0.0
37	7.506549464407695E-4	0.0	0.0	3.1365580754922955	0.0
38	7.506549464407695E-4	0.0	0.0	3.1821335543833422	0.0
39	8.578913673608795E-4	0.0	0.0	3.2272800875907084	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAC	20	7.0326E-4	45.000004	39
ATTAACG	20	7.0326E-4	45.000004	1
ACTACCG	25	3.8902384E-5	45.0	1
AACACGT	435	0.0	40.344826	41
CGTTTTT	3365	0.0	40.118874	1
TCGACGG	45	1.927583E-8	40.000004	2
TCAAGCG	455	0.0	39.065933	17
TAATACG	75	0.0	39.000004	4
ACACGTG	465	0.0	38.225807	42
ACACGAC	465	0.0	38.225807	26
CACGTGA	465	0.0	38.225807	43
CGTTATT	265	0.0	38.207546	1
AAACACG	460	0.0	38.152172	40
GCGATTA	130	0.0	38.07692	9
CGAAAGG	215	0.0	37.67442	2
CGACCAA	480	0.0	37.499996	29
TCCGTTA	30	1.1397568E-4	37.499996	12
GCGAGAC	465	0.0	37.258064	21
CACGACC	480	0.0	37.031246	27
CGAGACA	465	0.0	36.774193	22
>>END_MODULE