FastQCFastQC Report
Sat 18 Jun 2016
SRR3550286_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550286_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences644263
Sequences flagged as poor quality0
Sequence length51
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT491817.633683759582654No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA10780.16732297214025948No Hit
GCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC10600.16452908206741657No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA9490.14730009328488522No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA9150.14202274536951526No Hit
CCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC8560.13286499457519677No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA7710.11967162478677186No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAATTTTTTTTTTTTTT7260.11268689960466456No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAA6700.1039947971558199No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCGC253.8888895E-545.00000427
AGCTTGA253.8888895E-545.00000428
CGCGCAA253.8888895E-545.00000418
GCGATAT302.1640099E-645.0000049
GCAATTG302.1640099E-645.0000041
CGATATG302.1640099E-645.00000410
CGAACTA207.030976E-445.031
ACCCTAC207.030976E-445.016
GCGTACG207.030976E-445.01
CGTTTTT282000.044.5851061
CACGACC556.002665E-1140.90909227
AACACGT556.002665E-1140.90909241
GTTTTTT311100.040.631632
CGTAAGG501.0804797E-940.5000042
TAGACGG403.4550612E-739.3752
GTAAGTC403.4550612E-739.37510
TACGGGA1000.038.2500044
GGGCGAT5100.037.9411777
AGGGCGA2500.037.86
GCGCAAT301.139365E-437.50000419