##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550286_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	644263
Sequences flagged as poor quality	0
Sequence length	51
%GC	31
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.26741563616101	33.0	31.0	34.0	30.0	34.0
2	32.385233980532796	34.0	31.0	34.0	31.0	34.0
3	32.32321893388259	34.0	31.0	34.0	30.0	34.0
4	35.972775093401296	37.0	35.0	37.0	35.0	37.0
5	36.038457896852684	37.0	35.0	37.0	35.0	37.0
6	35.99626084378585	37.0	35.0	37.0	35.0	37.0
7	36.23032208896056	37.0	37.0	37.0	35.0	37.0
8	36.080048054909255	37.0	37.0	37.0	35.0	37.0
9	38.0145980756306	39.0	39.0	39.0	35.0	39.0
10	37.60546702200809	39.0	38.0	39.0	35.0	39.0
11	37.55935076203352	39.0	37.0	39.0	35.0	39.0
12	37.541598694942905	39.0	37.0	39.0	35.0	39.0
13	37.50861371831069	39.0	37.0	39.0	35.0	39.0
14	38.8300368017409	41.0	38.0	41.0	35.0	41.0
15	38.93423182768528	41.0	38.0	41.0	35.0	41.0
16	38.84394882214251	41.0	38.0	41.0	35.0	41.0
17	38.79325523893193	41.0	38.0	41.0	34.0	41.0
18	38.017123441824225	39.0	38.0	40.0	34.0	41.0
19	37.17279434019958	37.0	37.0	40.0	34.0	41.0
20	36.448551290389176	35.0	35.0	40.0	34.0	41.0
21	36.33616085356446	35.0	35.0	40.0	33.0	41.0
22	36.379799864341116	35.0	35.0	40.0	33.0	41.0
23	36.38863321345475	35.0	35.0	40.0	33.0	41.0
24	36.25877475503017	35.0	35.0	40.0	33.0	41.0
25	36.04925783414537	35.0	35.0	40.0	33.0	41.0
26	36.09795533811502	35.0	35.0	40.0	33.0	41.0
27	36.05395467379005	35.0	35.0	40.0	33.0	41.0
28	35.86822151823091	35.0	35.0	40.0	33.0	41.0
29	35.6507326976716	35.0	35.0	40.0	33.0	41.0
30	35.10808318962908	35.0	35.0	40.0	31.0	41.0
31	34.41824068742113	35.0	35.0	40.0	23.0	41.0
32	33.33046907862162	35.0	33.0	40.0	18.0	41.0
33	31.90939880142116	35.0	31.0	40.0	12.0	41.0
34	30.738484128376143	35.0	23.0	40.0	7.0	41.0
35	30.02431305227834	35.0	21.0	40.0	7.0	41.0
36	29.555543310728694	35.0	18.0	40.0	7.0	41.0
37	29.283089359469656	35.0	18.0	40.0	7.0	41.0
38	29.07349948701074	35.0	18.0	40.0	7.0	41.0
39	28.889644446445008	35.0	18.0	40.0	7.0	41.0
40	28.71984732942913	35.0	18.0	40.0	7.0	41.0
41	28.50578257637021	35.0	17.0	40.0	7.0	41.0
42	28.343103670395475	35.0	15.0	40.0	7.0	41.0
43	28.180285069917097	35.0	15.0	40.0	7.0	41.0
44	27.96989893878742	35.0	15.0	40.0	7.0	41.0
45	27.96599680565235	35.0	15.0	40.0	7.0	41.0
46	27.883757099197066	35.0	15.0	39.0	7.0	41.0
47	27.902653109056395	35.0	15.0	39.0	7.0	41.0
48	27.873849654566534	35.0	15.0	39.0	7.0	41.0
49	27.823587572156093	35.0	15.0	39.0	7.0	41.0
50	27.731707392788348	34.0	15.0	39.0	7.0	41.0
51	27.069390916442508	33.0	15.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	6.0
12	7.0
13	10.0
14	22.0
15	47.0
16	78.0
17	220.0
18	443.0
19	835.0
20	1457.0
21	2161.0
22	3284.0
23	5540.0
24	10158.0
25	21046.0
26	36837.0
27	46635.0
28	43667.0
29	34869.0
30	28039.0
31	23685.0
32	20402.0
33	20990.0
34	32829.0
35	42067.0
36	41453.0
37	42103.0
38	76262.0
39	109066.0
40	41.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.500495915487928	11.20660351440328	13.564646735882707	51.72825383422609
2	62.5730485841962	13.08580502062046	14.032157674738421	10.308988720444912
3	18.663030470475565	13.243815025851244	58.100806658150475	9.992347845522714
4	17.08634517270121	14.614528538811014	57.178512501882	11.12061378660578
5	14.348177685199989	17.02782869728667	56.9733167976432	11.650676819870148
6	15.426929685547671	19.503370517940656	56.58527650974835	8.484423286763326
7	46.16049656739561	3.420497529735527	47.64312089938426	2.7758850034846017
8	46.309504037947235	2.993653213051192	47.876255504351484	2.8205872446500884
9	42.978566206657845	4.577168019892498	48.999554529749496	3.4447112437001657
10	24.563726304319818	14.285625590791337	53.04277911349868	8.107868991390161
11	18.840908138446565	14.570602378221317	56.72435014892986	9.864139334402255
12	17.94158596722146	13.2019066747586	58.24857239978084	10.607934958239104
13	14.82282856535297	13.932198496576708	58.936179789930506	12.308793148139813
14	11.50648104888842	15.440278271451255	59.48611048593509	13.567130193725232
15	11.725646203491431	15.523163676945595	61.346375626103	11.40481449345997
16	13.754165612490551	15.539616585152338	59.87787596059373	10.828341841763379
17	13.87849372073206	15.5379092078856	58.43312436070362	12.150472710678715
18	13.617420215036406	15.892888463251808	58.67464063588939	11.815050685822404
19	14.242785943007746	16.326407072887935	57.88210715189294	11.54869983221138
20	15.871002991014539	15.506555552623697	58.352101548591186	10.270339907770584
21	15.10082062760084	15.694522268079961	58.39866638313856	10.805990721180637
22	14.325516132386928	14.967800416910485	58.52252884303461	12.184154607667987
23	13.928473309812919	14.691205299699037	59.29814377047883	12.08217762000922
24	14.093002391880335	13.970847309251036	59.60966251360082	12.326487785267817
25	13.031168948084865	15.352581166387019	58.10453184491426	13.511718040613848
26	12.448642867897117	16.481623188045877	58.459045451935	12.610688492122005
27	12.548602046058829	16.505836902010515	58.705373426690656	12.240187625240003
28	11.290109784358252	16.840948494636507	58.990505430235785	12.878436290769452
29	12.553413745628724	16.03227253466364	58.62171194062052	12.792601779087112
30	13.27656562614957	15.657891264902688	57.8774506684382	13.188092440509545
31	13.663364185123156	16.86873217924978	56.30371447685184	13.164189158775223
32	14.692757460850617	18.080504390287818	53.65479625556644	13.571941893295131
33	15.294685555433107	20.36947644052196	50.577326340329954	13.758511663714973
34	14.194668947308786	21.926914940016733	49.54451831006902	14.333897802605458
35	14.763691225477793	23.764673743486746	45.585265644620286	15.886369386415176
36	15.649509594684158	26.49896082810902	42.85516939510728	14.996360182099547
37	16.692096240200044	25.503094233255673	43.28667019524635	14.518139331297933
38	16.78801979936765	25.471585361878613	42.21629986511719	15.524094973636544
39	17.175284006686713	24.131449423604955	42.20481387259551	16.488452697112823
40	17.575896799909355	23.740460029522108	42.611324878194154	16.072318292374387
41	15.926570360241083	25.04349931627301	41.452170930194654	17.57775939329125
42	16.8671800180982	25.217651797480222	40.4415588044013	17.47360938002027
43	17.3100115946438	25.233328625111174	40.4013578305754	17.05530194966962
44	16.724070759922576	25.0989114693844	40.39018227028403	17.786835500408994
45	16.950531071938013	24.30451539200606	40.85520975129722	17.889743784758707
46	15.960717905575827	25.097048876002503	41.30626778194619	17.635965436475477
47	15.886679818645492	24.016123850042607	42.41528692474967	17.681909406562227
48	15.52580235090328	22.684059149757164	43.660275384431515	18.129863114908044
49	16.178020466796944	22.821425411671942	43.21759902400107	17.782955097530046
50	14.96003961115259	22.28670589495284	43.60222455736245	19.15102993653213
51	14.72938846402789	21.88857655957272	43.1115864173482	20.270448559051196
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	178.0
1	603.0
2	1028.0
3	48332.5
4	95637.0
5	63040.0
6	30443.0
7	28761.0
8	27079.0
9	25959.5
10	24840.0
11	23523.5
12	22207.0
13	20249.5
14	18292.0
15	16833.5
16	15375.0
17	14052.5
18	12730.0
19	11632.0
20	10534.0
21	9496.5
22	8459.0
23	7825.0
24	7191.0
25	6996.0
26	6776.0
27	6751.0
28	6968.5
29	7186.0
30	7666.0
31	8146.0
32	9038.0
33	9930.0
34	10626.5
35	11323.0
36	12207.0
37	13091.0
38	14253.0
39	15415.0
40	16445.0
41	17475.0
42	18525.0
43	19575.0
44	21946.5
45	24318.0
46	25640.0
47	26962.0
48	29050.5
49	31139.0
50	30914.5
51	30690.0
52	27764.0
53	24838.0
54	23098.0
55	21358.0
56	20186.0
57	19014.0
58	18857.5
59	18701.0
60	18202.0
61	17703.0
62	15915.5
63	14128.0
64	12039.0
65	9950.0
66	7884.5
67	5819.0
68	4672.0
69	3525.0
70	3111.5
71	2698.0
72	2154.5
73	1611.0
74	1315.5
75	798.5
76	577.0
77	394.5
78	212.0
79	185.0
80	158.0
81	118.5
82	79.0
83	55.5
84	32.0
85	29.0
86	26.0
87	17.0
88	8.0
89	7.5
90	7.0
91	3.5
92	0.0
93	1.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	644263.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.01693253801051
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.8002244096303	31.915938373846657
2	7.367646254321169	5.749259137375497
3	2.4476028871213353	2.8649387017995864
4	1.1891743538855866	1.8559174216594465
5	0.7813466569060166	1.5242874900651042
6	0.5665002225920537	1.3261860580585256
7	0.4531903361116031	1.2377467739661308
8	0.39345401751087383	1.2281095086424774
9	0.35636481769347056	1.25138358457799
>10	4.487698171006414	34.816280600469
>50	0.08277654221874578	2.2940092423231717
>100	0.06805186882478093	4.701989160819693
>500	0.00477556974208989	1.2654782266917803
>1k	7.959282903483149E-4	0.33197453357877627
>5k	0.0	0.0
>10k+	3.9796414517415747E-4	7.636501186126191
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	49181	7.633683759582654	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1078	0.16732297214025948	No Hit
GCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC	1060	0.16452908206741657	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	949	0.14730009328488522	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	915	0.14202274536951526	No Hit
CCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC	856	0.13286499457519677	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA	771	0.11967162478677186	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAATTTTTTTTTTTTTT	726	0.11268689960466456	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAA	670	0.1039947971558199	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.104322303158803E-4	0.0	0.0	0.10042482650718729	0.0
2	3.104322303158803E-4	0.0	0.0	0.5193531213184678	0.0
3	3.104322303158803E-4	0.0	0.0	0.6942816831014663	0.0
4	3.104322303158803E-4	0.0	0.0	0.8926478782733138	0.0
5	3.104322303158803E-4	0.0	0.0	1.3910468240454597	0.0
6	3.104322303158803E-4	0.0	0.0	1.6507233847046936	0.0
7	3.104322303158803E-4	0.0	0.0	1.9225068023462468	0.0
8	3.104322303158803E-4	0.0	0.0	2.3332086430541565	0.0
9	6.208644606317607E-4	0.0	0.0	2.4640558281323	0.0
10	6.208644606317607E-4	0.0	0.0	2.7789893257877605	0.0
11	6.208644606317607E-4	0.0	0.0	3.3110701685491795	0.0
12	6.208644606317607E-4	0.0	0.0	3.693367460183186	0.0
13	6.208644606317607E-4	0.0	0.0	3.8636395385114466	0.0
14	6.208644606317607E-4	0.0	0.0	3.928519874647465	0.0
15	6.208644606317607E-4	0.0	0.0	4.067748729944138	0.0
16	7.760805757897008E-4	0.0	0.0	4.3637458615503295	0.0
17	7.760805757897008E-4	0.0	0.0	4.704755666552324	0.0
18	7.760805757897008E-4	0.0	0.0	5.067340511561272	0.0
19	7.760805757897008E-4	0.0	0.0	5.315065431353345	0.0
20	7.760805757897008E-4	0.0	0.0	5.5685333474062615	0.0
21	7.760805757897008E-4	0.0	0.0	5.883001196716248	0.0
22	7.760805757897008E-4	0.0	0.0	6.192812562571496	0.0
23	7.760805757897008E-4	0.0	0.0	6.490361855329268	0.0
24	7.760805757897008E-4	0.0	0.0	6.73436158835755	0.0
25	7.760805757897008E-4	0.0	0.0	6.957872794184984	0.0
26	7.760805757897008E-4	0.0	0.0	7.161671553387359	0.0
27	7.760805757897008E-4	0.0	0.0	7.361124261365312	0.0
28	7.760805757897008E-4	0.0	0.0	7.576253796974217	0.0
29	7.760805757897008E-4	0.0	0.0	7.799454570571335	0.0
30	9.31296690947641E-4	0.0	0.0	8.047179490363407	0.0
31	9.31296690947641E-4	0.0	0.0	8.295525274616113	0.0
32	0.0010865128061055811	0.0	0.0	8.537817630377656	0.0
33	0.0012417289212635213	0.0	0.0	8.76955529030846	0.0
34	0.0012417289212635213	0.0	0.0	8.998188627936107	0.0
35	0.0012417289212635213	0.0	0.0	9.224183291606067	0.0
36	0.0012417289212635213	0.0	0.0	9.473615588664877	0.0
37	0.0012417289212635213	0.0	0.0	9.711251460971685	0.0
38	0.0012417289212635213	0.0	0.0	9.948266468817858	0.0
39	0.0013969450364214615	0.0	0.0	10.204062626598144	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTGCGC	25	3.8888895E-5	45.000004	27
AGCTTGA	25	3.8888895E-5	45.000004	28
CGCGCAA	25	3.8888895E-5	45.000004	18
GCGATAT	30	2.1640099E-6	45.000004	9
GCAATTG	30	2.1640099E-6	45.000004	1
CGATATG	30	2.1640099E-6	45.000004	10
CGAACTA	20	7.030976E-4	45.0	31
ACCCTAC	20	7.030976E-4	45.0	16
GCGTACG	20	7.030976E-4	45.0	1
CGTTTTT	28200	0.0	44.585106	1
CACGACC	55	6.002665E-11	40.909092	27
AACACGT	55	6.002665E-11	40.909092	41
GTTTTTT	31110	0.0	40.63163	2
CGTAAGG	50	1.0804797E-9	40.500004	2
TAGACGG	40	3.4550612E-7	39.375	2
GTAAGTC	40	3.4550612E-7	39.375	10
TACGGGA	100	0.0	38.250004	4
GGGCGAT	510	0.0	37.941177	7
AGGGCGA	250	0.0	37.8	6
GCGCAAT	30	1.139365E-4	37.500004	19
>>END_MODULE