FastQCFastQC Report
Sat 18 Jun 2016
SRR3550285_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550285_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences408807
Sequences flagged as poor quality0
Sequence length51
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT301907.384902900390649No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA6360.15557463546367845No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA5680.13894086940781347No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA5500.13453781368714332No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA5300.12964552955306538No Hit
GCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGC4660.11399022032401598Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAA4420.10811947936312245No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCATC207.027948E-445.08
GTACCGG207.027948E-445.03
CACGGGC253.886381E-545.04
CCGATAG253.886381E-545.011
GGCCGTA207.027948E-445.011
TAGCGCG253.886381E-545.01
CGATTGT207.027948E-445.010
CACGAGG253.886381E-545.02
TCGATGG253.886381E-545.02
AACGGCA207.027948E-445.017
TACGAGG351.2093551E-745.02
GCGGGAC253.886381E-545.05
CTTGACG207.027948E-445.01
ACTAATC502.1827873E-1145.010
GGCCTAT207.027948E-445.08
CGATAGT207.027948E-445.038
CGCGAGG600.044.9999962
TGCGCGG302.162058E-644.9999962
CGTTTTT181000.044.589781
GTTTTTT198500.040.9307332