##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550285_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	408807
Sequences flagged as poor quality	0
Sequence length	51
%GC	31
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.342753426433504	33.0	31.0	34.0	31.0	34.0
2	32.450914490211765	34.0	31.0	34.0	31.0	34.0
3	32.390707595515735	34.0	31.0	34.0	30.0	34.0
4	36.02596335189955	37.0	35.0	37.0	35.0	37.0
5	36.084100810406866	37.0	35.0	37.0	35.0	37.0
6	36.03463003324307	37.0	35.0	37.0	35.0	37.0
7	36.253628240220934	37.0	37.0	37.0	35.0	37.0
8	36.10624818068184	37.0	37.0	37.0	35.0	37.0
9	38.05039297272307	39.0	39.0	39.0	35.0	39.0
10	37.67965568104265	39.0	38.0	39.0	35.0	39.0
11	37.64685046978158	39.0	38.0	39.0	35.0	39.0
12	37.641204773890856	39.0	37.0	39.0	35.0	39.0
13	37.597125293842815	39.0	38.0	39.0	35.0	39.0
14	38.94941378205363	41.0	38.0	41.0	35.0	41.0
15	39.067623597443294	41.0	39.0	41.0	35.0	41.0
16	38.98626246615151	41.0	38.0	41.0	35.0	41.0
17	38.94610415183693	41.0	38.0	41.0	35.0	41.0
18	38.16464982253239	39.0	38.0	40.0	35.0	41.0
19	37.29779578138339	37.0	37.0	40.0	35.0	41.0
20	36.536238371652146	35.0	35.0	40.0	34.0	41.0
21	36.41877462959294	35.0	35.0	40.0	33.0	41.0
22	36.46164571545986	35.0	35.0	40.0	33.0	41.0
23	36.460075292252824	35.0	35.0	40.0	33.0	41.0
24	36.31808652982948	35.0	35.0	40.0	33.0	41.0
25	36.11655377721027	35.0	35.0	40.0	33.0	41.0
26	36.14895537503027	35.0	35.0	40.0	33.0	41.0
27	36.12245631801804	35.0	35.0	40.0	33.0	41.0
28	35.92203411389727	35.0	35.0	40.0	33.0	41.0
29	35.68479747166756	35.0	35.0	40.0	33.0	41.0
30	35.1541705499172	35.0	35.0	40.0	31.0	41.0
31	34.427485341493664	35.0	35.0	40.0	23.0	41.0
32	33.30298160256552	35.0	33.0	40.0	17.0	41.0
33	31.834359489930456	35.0	31.0	40.0	10.0	41.0
34	30.602521483242704	35.0	23.0	40.0	7.0	41.0
35	29.824770613027663	35.0	20.0	40.0	7.0	41.0
36	29.294906887602217	35.0	18.0	40.0	7.0	41.0
37	28.990313277414526	35.0	18.0	40.0	7.0	41.0
38	28.76757491921616	35.0	17.0	40.0	7.0	41.0
39	28.55879913993645	35.0	16.0	40.0	7.0	41.0
40	28.389491862908415	35.0	15.0	40.0	7.0	41.0
41	28.17465698972865	35.0	15.0	40.0	7.0	41.0
42	28.03122500348575	35.0	15.0	40.0	7.0	41.0
43	27.85339781363822	35.0	15.0	40.0	7.0	41.0
44	27.679714388452254	35.0	14.0	40.0	7.0	41.0
45	27.715772968662474	35.0	15.0	40.0	7.0	41.0
46	27.64381480747639	35.0	15.0	40.0	7.0	41.0
47	27.667982691098732	35.0	15.0	39.0	7.0	41.0
48	27.652393427705494	34.0	15.0	39.0	7.0	41.0
49	27.571210864784604	34.0	15.0	39.0	7.0	41.0
50	27.489081644883772	34.0	15.0	39.0	7.0	41.0
51	26.889833099726765	33.0	15.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	4.0
10	5.0
11	2.0
12	5.0
13	8.0
14	13.0
15	33.0
16	63.0
17	124.0
18	272.0
19	581.0
20	940.0
21	1428.0
22	2173.0
23	3538.0
24	6686.0
25	13476.0
26	24670.0
27	32086.0
28	29643.0
29	22351.0
30	16678.0
31	13585.0
32	11733.0
33	11990.0
34	18767.0
35	25077.0
36	25547.0
37	25881.0
38	47574.0
39	73843.0
40	28.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.39345216691495	11.920050292680898	13.435190689004836	52.25130685139931
2	62.96222912034285	13.701330945898677	13.585628426127732	9.75081150763074
3	18.43901890133975	13.292335992289761	58.45961541754422	9.809029688826268
4	16.980629000971117	14.480916422663995	57.90287348308615	10.635581093278736
5	14.865694569809227	16.704948790015827	57.420005039052654	11.00935160112229
6	15.27786950810529	19.413317286641373	56.899710621393474	8.409102583859864
7	44.986754140706985	3.8998842974802295	48.414777633455394	2.698583928357391
8	45.80719018999185	3.592404239653431	48.1574434880029	2.4429620823518188
9	42.29232865386356	5.0674279060779295	49.314468685712335	3.325774754346183
10	24.693804166758397	14.460613443507572	53.2229144804272	7.622667909306837
11	18.38251301959115	15.333152318820373	56.559207645661644	9.72512701592683
12	18.238924480255964	13.460875180708745	58.073369585158765	10.226830753876524
13	14.987512444747766	14.48556409259137	58.63573764637102	11.891185816289838
14	11.090074289334575	16.620556888702982	58.86200578757214	13.42736303439031
15	11.033323793379273	17.11418835783145	61.14547940715301	10.707008441636273
16	12.353751281166907	14.937855760786874	61.50677459045466	11.201618367591552
17	12.574393295613822	15.82311457484828	59.06772633541011	12.534765794127791
18	12.444013923440647	16.623247644976725	58.88548875141571	12.047249680166924
19	14.497794802926563	16.979895158351006	57.67831764133198	10.843992397390457
20	16.46669455268623	15.20619754554105	58.57580716572856	9.751300736044149
21	14.816527114261744	15.746794942356662	58.584857891376615	10.85182005200498
22	14.09393674765843	15.184426881144402	58.3519851665945	12.369651204602661
23	13.750253787239455	15.930500211591289	58.974283708449214	11.344962292720037
24	13.34125883363054	14.716969132133256	59.780042905331854	12.161729128904348
25	13.019346537608211	15.883778898110846	58.72624490285146	12.370629661429476
26	13.137739813652896	17.529787895021368	57.95326400966715	11.379208281658581
27	12.950365331317713	17.621273608328625	58.585836348203436	10.842524712150233
28	12.329045246289814	17.14036207794876	58.76122473441012	11.769367941351298
29	12.761033935328896	16.258527862781214	58.66044368124812	12.31999452064177
30	13.015677324507651	17.219372466714123	57.06971749505267	12.695232713725549
31	13.185439583960157	18.42434204893752	55.48608512085165	12.904133246250677
32	14.590503587267342	19.61218863669164	53.72681974623722	12.070488029803796
33	15.011973865418154	21.745224519149623	50.14640160271229	13.096400012719938
34	14.692507711462866	23.402485769568525	47.684848840650965	14.22015767831764
35	15.862986690541014	24.02160432673609	44.64356040870142	15.471848574021482
36	15.887937339624811	26.350820802970592	42.50343071424902	15.257811143155573
37	17.078719297859383	26.85472606878062	42.125012536478096	13.941542096881903
38	17.117368342518596	25.91981057075833	41.35594547060104	15.606875616122032
39	17.179011122607978	25.761300564814206	41.48754791380774	15.57214039877008
40	17.143052834222505	26.90218122488118	41.349096272813334	14.605669668082982
41	15.860540548473976	27.11132637161301	41.35276548591389	15.675367593999123
42	16.766346955898502	27.31900383310462	39.45455924189165	16.460089969105226
43	17.355133351434784	27.23705807385881	39.354512031349756	16.053296543356645
44	16.33680440892646	27.63920382968002	39.20407429422686	16.819917467166658
45	16.206669650959988	26.409038984166123	39.854748084059224	17.529543280814664
46	15.677813736066163	26.750275802518058	39.99148742560671	17.580423035809073
47	15.864943604194645	26.148769468233173	41.87795218770716	16.108334739865022
48	16.181963616082896	24.451146873708133	42.16610772320435	17.200781787004626
49	15.14284246600474	24.743216236512584	42.34565455092501	17.768286746557667
50	14.709141477518731	24.658335106786332	42.857142857142854	17.77538055855208
51	14.534731548138852	23.7461687299875	42.1127818261429	19.606317895730747
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	99.0
1	417.0
2	735.0
3	28884.0
4	57033.0
5	39054.5
6	21076.0
7	20050.0
8	19024.0
9	18168.0
10	17312.0
11	16176.5
12	15041.0
13	13876.5
14	12712.0
15	11565.5
16	10419.0
17	9469.5
18	8520.0
19	7826.5
20	7133.0
21	6352.5
22	5572.0
23	5114.0
24	4656.0
25	4634.0
26	4523.0
27	4434.0
28	4876.5
29	5319.0
30	5673.0
31	6027.0
32	6253.0
33	6479.0
34	6950.0
35	7421.0
36	7994.5
37	8568.0
38	9389.5
39	10211.0
40	11305.5
41	12400.0
42	13227.5
43	14055.0
44	14595.5
45	15136.0
46	15968.0
47	16800.0
48	17041.5
49	17283.0
50	16748.5
51	16214.0
52	14665.0
53	13116.0
54	12487.5
55	11859.0
56	11043.5
57	10228.0
58	10569.0
59	10910.0
60	11335.0
61	11760.0
62	10676.5
63	9593.0
64	7911.5
65	6230.0
66	5007.0
67	3784.0
68	3074.0
69	2364.0
70	2117.0
71	1870.0
72	1518.5
73	1167.0
74	945.0
75	603.5
76	484.0
77	342.0
78	200.0
79	166.5
80	133.0
81	87.0
82	41.0
83	35.0
84	29.0
85	19.0
86	9.0
87	10.5
88	12.0
89	7.5
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	408807.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.66693984948417
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.09396741678707	32.56416467527206
2	6.4089553355995585	5.084472915905166
3	2.2178999570174915	2.639319125615591
4	1.184340098083194	1.8791658972799294
5	0.8774254358381904	1.7402390992900467
6	0.6783607697797713	1.614509751066238
7	0.5100546952215471	1.4162616240710064
8	0.5040931454322568	1.5996665982718883
9	0.46290653887608246	1.652587725017742
>10	4.901638666063084	35.064292010968565
>50	0.09991533265724832	2.7997784786374047
>100	0.05735880208101292	4.000766992411524
>500	0.0024670452507962546	0.5587813741006494
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	6.167613126990636E-4	7.385993732092209
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30190	7.384902900390649	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	636	0.15557463546367845	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	568	0.13894086940781347	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	550	0.13453781368714332	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA	530	0.12964552955306538	No Hit
GCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGC	466	0.11399022032401598	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAA	442	0.10811947936312245	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07925500297206262	0.0
2	0.0	0.0	0.0	0.38844736024578835	0.0
3	0.0	0.0	0.0	0.5139344482848874	0.0
4	0.0	0.0	0.0	0.6641275712010802	0.0
5	0.0	0.0	0.0	1.0496395609664217	0.0
6	0.0	0.0	0.0	1.253403195150768	0.0
7	0.0	0.0	0.0	1.4559437583015946	0.0
8	0.0	0.0	0.0	1.720860944161915	0.0
9	0.0	0.0	0.0	1.8248219820110712	0.0
10	0.0	0.0	0.0	2.0432624685976513	0.0
11	2.4461420670389693E-4	0.0	0.0	2.4255944736758424	0.0
12	2.4461420670389693E-4	0.0	0.0	2.7191315217205183	0.0
13	2.4461420670389693E-4	0.0	0.0	2.846086294999841	0.0
14	2.4461420670389693E-4	0.0	0.0	2.900146034681402	0.0
15	2.4461420670389693E-4	0.0	0.0	3.0001932452232962	0.0
16	2.4461420670389693E-4	0.0	0.0	3.2056691788545697	0.0
17	2.4461420670389693E-4	0.0	0.0	3.457621811759583	0.0
18	2.4461420670389693E-4	0.0	0.0	3.69905603377633	0.0
19	4.892284134077939E-4	0.0	0.0	3.859522953374086	0.0
20	4.892284134077939E-4	0.0	0.0	4.022436015038881	0.0
21	4.892284134077939E-4	0.0	0.0	4.244056486312612	0.0
22	4.892284134077939E-4	0.0	0.0	4.467144642826566	0.0
23	4.892284134077939E-4	0.0	0.0	4.682405144725996	0.0
24	4.892284134077939E-4	0.0	0.0	4.848987419491349	0.0
25	4.892284134077939E-4	0.0	0.0	5.010188181709217	0.0
26	4.892284134077939E-4	0.0	0.0	5.150107507943846	0.0
27	4.892284134077939E-4	0.0	0.0	5.290026834178476	0.0
28	4.892284134077939E-4	0.0	0.0	5.4394861144745565	0.0
29	7.338426201116908E-4	0.0	0.0	5.599708419865609	0.0
30	7.338426201116908E-4	0.0	0.0	5.7758306486924145	0.0
31	7.338426201116908E-4	0.0	0.0	5.959046689513634	0.0
32	7.338426201116908E-4	0.0	0.0	6.142018116128148	0.0
33	7.338426201116908E-4	0.0	0.0	6.310801918753837	0.0
34	7.338426201116908E-4	0.0	0.0	6.475182665658856	0.0
35	7.338426201116908E-4	0.0	0.0	6.649103366625327	0.0
36	9.784568268155877E-4	0.0	0.0	6.844060889368333	0.0
37	9.784568268155877E-4	0.0	0.0	7.047579909345975	0.0
38	9.784568268155877E-4	0.0	0.0	7.210248356804066	0.0
39	9.784568268155877E-4	0.0	0.0	7.386615199837576	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGCATC	20	7.027948E-4	45.0	8
GTACCGG	20	7.027948E-4	45.0	3
CACGGGC	25	3.886381E-5	45.0	4
CCGATAG	25	3.886381E-5	45.0	11
GGCCGTA	20	7.027948E-4	45.0	11
TAGCGCG	25	3.886381E-5	45.0	1
CGATTGT	20	7.027948E-4	45.0	10
CACGAGG	25	3.886381E-5	45.0	2
TCGATGG	25	3.886381E-5	45.0	2
AACGGCA	20	7.027948E-4	45.0	17
TACGAGG	35	1.2093551E-7	45.0	2
GCGGGAC	25	3.886381E-5	45.0	5
CTTGACG	20	7.027948E-4	45.0	1
ACTAATC	50	2.1827873E-11	45.0	10
GGCCTAT	20	7.027948E-4	45.0	8
CGATAGT	20	7.027948E-4	45.0	38
CGCGAGG	60	0.0	44.999996	2
TGCGCGG	30	2.162058E-6	44.999996	2
CGTTTTT	18100	0.0	44.58978	1
GTTTTTT	19850	0.0	40.930733	2
>>END_MODULE