##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550284_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	520148
Sequences flagged as poor quality	0
Sequence length	51
%GC	31
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.28525919545975	33.0	31.0	34.0	30.0	34.0
2	32.40305836031283	34.0	31.0	34.0	31.0	34.0
3	32.32929473918961	34.0	31.0	34.0	30.0	34.0
4	35.97275775356245	37.0	35.0	37.0	35.0	37.0
5	36.04110945346324	37.0	35.0	37.0	35.0	37.0
6	35.99342879334343	37.0	35.0	37.0	35.0	37.0
7	36.22657974268862	37.0	37.0	37.0	35.0	37.0
8	36.07961003406723	37.0	37.0	37.0	35.0	37.0
9	38.012092712074256	39.0	39.0	39.0	35.0	39.0
10	37.63273914347455	39.0	38.0	39.0	35.0	39.0
11	37.595488207202564	39.0	37.0	39.0	35.0	39.0
12	37.612106554288395	39.0	37.0	39.0	35.0	39.0
13	37.58763275067865	39.0	37.0	39.0	35.0	39.0
14	38.913217007467104	41.0	38.0	41.0	35.0	41.0
15	39.029991463967946	41.0	39.0	41.0	35.0	41.0
16	38.942585571798794	41.0	38.0	41.0	35.0	41.0
17	38.90475403154487	41.0	38.0	41.0	35.0	41.0
18	38.121248183209396	39.0	38.0	40.0	35.0	41.0
19	37.25190907203334	37.0	37.0	40.0	34.0	41.0
20	36.49415743211547	35.0	35.0	40.0	34.0	41.0
21	36.38768196744004	35.0	35.0	40.0	33.0	41.0
22	36.43075624629913	35.0	35.0	40.0	33.0	41.0
23	36.42179533517383	35.0	35.0	40.0	33.0	41.0
24	36.283334743188476	35.0	35.0	40.0	33.0	41.0
25	36.06047509554973	35.0	35.0	40.0	33.0	41.0
26	36.1054738266801	35.0	35.0	40.0	33.0	41.0
27	36.06883809992541	35.0	35.0	40.0	33.0	41.0
28	35.87405123157255	35.0	35.0	40.0	33.0	41.0
29	35.63964102524666	35.0	35.0	40.0	33.0	41.0
30	35.098416219998924	35.0	35.0	40.0	31.0	41.0
31	34.42578458438752	35.0	35.0	40.0	23.0	41.0
32	33.304765182217366	35.0	33.0	40.0	18.0	41.0
33	31.83457208333013	35.0	31.0	40.0	10.0	41.0
34	30.617704960895747	35.0	23.0	40.0	7.0	41.0
35	29.84243907503249	35.0	21.0	40.0	7.0	41.0
36	29.335429531594855	35.0	18.0	40.0	7.0	41.0
37	29.011141059852196	35.0	18.0	40.0	7.0	41.0
38	28.789609880264848	35.0	18.0	40.0	7.0	41.0
39	28.57888524035467	35.0	16.0	40.0	7.0	41.0
40	28.384936594969123	35.0	15.0	40.0	7.0	41.0
41	28.14710620823304	35.0	15.0	40.0	7.0	41.0
42	27.988999284818934	35.0	15.0	40.0	7.0	41.0
43	27.820499165622092	35.0	15.0	40.0	7.0	41.0
44	27.635649853503235	35.0	12.0	40.0	7.0	41.0
45	27.675644239716387	35.0	15.0	40.0	7.0	41.0
46	27.609739920176565	35.0	15.0	39.0	7.0	41.0
47	27.647763713404647	34.0	15.0	39.0	7.0	41.0
48	27.61232572267893	34.0	15.0	39.0	7.0	41.0
49	27.536355037412427	34.0	15.0	39.0	7.0	41.0
50	27.457054530633588	34.0	15.0	39.0	7.0	41.0
51	26.843459938325246	33.0	15.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	5.0
11	6.0
12	7.0
13	15.0
14	20.0
15	49.0
16	84.0
17	197.0
18	423.0
19	741.0
20	1202.0
21	1847.0
22	2916.0
23	4467.0
24	8407.0
25	17177.0
26	31502.0
27	39670.0
28	36668.0
29	29117.0
30	22005.0
31	17621.0
32	15640.0
33	16043.0
34	23838.0
35	31784.0
36	34558.0
37	34403.0
38	60945.0
39	88754.0
40	33.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.79706545060252	11.837976883502389	13.529418550104971	52.83553911579012
2	63.208740589216916	12.965732829886878	13.93353430177565	9.891992279120558
3	18.52011350615594	13.248152448918384	58.58967063220468	9.642063412720994
4	16.366880195636625	14.314195190599598	58.249190614978815	11.069733998784962
5	14.672939240370047	16.450510239393402	57.893522612794825	10.983027907441729
6	15.200096895498975	19.46868968062936	56.90034374831778	8.43086967555388
7	45.06698093619509	3.623007297922899	48.627506017518094	2.682505748363927
8	45.60221321623846	3.3286679944938746	48.64557779708852	2.423540992179149
9	42.08917461953136	4.786137791551635	49.839661019555976	3.285026569361028
10	24.434391750040373	14.135976683559296	53.704330305989835	7.7253012604104985
11	17.77186493075048	15.237586225458907	56.95936541138291	10.0311834324077
12	17.969116482231982	13.583826141790414	58.1032706075963	10.343786768381307
13	14.30554380676269	13.893545683151718	59.120288840868376	12.680621669217224
14	11.400793620277305	16.277098056706937	59.30946576743542	13.012642555580333
15	11.390411959673017	16.046778993671033	61.33908041557403	11.223728631081922
16	12.504902450840916	14.71850319524443	61.60823457938894	11.168359774525712
17	12.563539607957736	15.511546713627661	59.2723609434238	12.65255273499081
18	12.651783723094196	16.049855041257487	59.22737374747188	12.070987488176442
19	14.443581442204911	15.83856902266278	58.52084406745772	11.197005467674586
20	15.98102847651053	14.940170874443428	59.17162038496736	9.907180264078686
21	14.809823357967348	15.288533263609589	58.71098225889555	11.190661119527519
22	13.815106469697087	14.92940470789083	58.83037135584488	12.425117466567208
23	14.18288640925275	15.097626060275152	59.20968647384976	11.509801056622345
24	13.578250805539962	14.372447841768112	59.63552681159977	12.41377454109215
25	12.732337719264516	15.844336611887385	58.29667709959473	13.126648569253366
26	12.966501841783492	17.062259203149875	58.3497389204611	11.621500034605535
27	12.955543422256744	17.096480232549197	58.72809277359521	11.219883571598853
28	11.994278551489192	16.623153410183257	58.97186954482186	12.410698493505695
29	12.864223259533825	15.988718595476673	58.62677545621631	12.520282688773197
30	13.23257995801195	16.855395002960698	57.28062013119343	12.631404907833923
31	13.222967309304275	17.72207141044472	55.44037466259603	13.614586617654975
32	14.766374185808653	18.997862146927414	53.44882610333982	12.786937563924115
33	14.395710451640687	21.390450410267846	50.754016164630066	13.4598229734614
34	14.562778286180086	22.5216669101871	47.74737190184332	15.16818290178949
35	15.629013280835455	23.396610195559724	44.583849212147314	16.390527311457507
36	15.681113836831056	26.573398340472327	42.19414474341918	15.551343079277435
37	16.71274329613879	26.21426978475357	42.202796127256086	14.87019079185155
38	16.13483085583334	26.092958158062707	41.576243684489796	16.195967301614157
39	16.710628513423103	25.856871505802193	41.57278313095504	15.859716849819666
40	17.1403139106562	25.27242246437552	41.85462599106408	15.732637633904195
41	15.600559840660736	26.70835992832809	40.89393787921899	16.797142351792182
42	16.305551496881655	26.679521982205063	39.71427362981305	17.300652891100228
43	17.018041019094564	26.95982681852088	39.55316563747241	16.46896652491214
44	16.125602713073974	27.302806124410743	39.58219583656959	16.98939532594569
45	15.829917638825872	25.91800795158301	39.77810161723202	18.4739727923591
46	15.110314756569284	26.174665672077946	40.583064820012765	18.131954751340004
47	15.57229865346017	25.738635926697782	41.92172227904366	16.76734314079839
48	15.5986373109192	24.111022247514168	42.590762629097874	17.69957781246876
49	15.0784007628598	24.53878511500573	42.75513892199913	17.627675200135347
50	14.500296069580196	23.807839307274083	42.96257988111076	18.72928474203496
51	14.354376062197682	23.029214761952367	42.69784753570138	19.918561640148575
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	139.0
1	529.0
2	919.0
3	37137.0
4	73355.0
5	49767.0
6	26179.0
7	25155.5
8	24132.0
9	22930.0
10	21728.0
11	20534.0
12	19340.0
13	17904.5
14	16469.0
15	15016.0
16	13563.0
17	12460.5
18	11358.0
19	10358.5
20	9359.0
21	8384.0
22	7409.0
23	6774.0
24	6139.0
25	5633.5
26	5036.0
27	4944.0
28	5414.0
29	5884.0
30	6446.5
31	7009.0
32	7815.0
33	8621.0
34	8828.5
35	9036.0
36	9731.5
37	10427.0
38	10881.0
39	11335.0
40	12555.5
41	13776.0
42	14736.0
43	15696.0
44	17342.5
45	18989.0
46	19598.0
47	20207.0
48	21928.0
49	23649.0
50	23133.5
51	22618.0
52	20547.0
53	18476.0
54	17354.5
55	16233.0
56	15263.5
57	14294.0
58	14150.5
59	14007.0
60	14347.0
61	14687.0
62	13629.5
63	12572.0
64	10261.0
65	7950.0
66	6621.5
67	5293.0
68	4239.5
69	3186.0
70	2732.5
71	2279.0
72	1962.0
73	1645.0
74	1345.5
75	767.5
76	489.0
77	342.0
78	195.0
79	164.5
80	134.0
81	133.0
82	132.0
83	101.5
84	71.0
85	45.5
86	20.0
87	22.5
88	25.0
89	14.0
90	3.0
91	2.0
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	520148.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.1623410618232
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.18107243836106	33.00584260095855
2	6.861420086629841	5.511413873753444
3	2.3316718321013075	2.809361980952966
4	1.2068139662890471	1.938738964490893
5	0.7521988558615063	1.5105033497711502
6	0.5945223885415132	1.432644656249641
7	0.507738152756136	1.4274367002764414
8	0.4345282438278751	1.3961337223688153
9	0.4094833271464926	1.4801228139588805
>10	4.5718987424276385	34.41301866242254
>50	0.08439595492303949	2.387506621775614
>100	0.059940308894187666	3.868311527383656
>500	0.00383617976922801	1.013202576200608
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	4.7952247115350127E-4	7.80576194943677
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40531	7.79220529541592	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	835	0.16053123341818099	No Hit
CCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTGC	783	0.15053407876219846	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	747	0.1436129716926721	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	693	0.13323131108838254	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA	616	0.11842783207856226	No Hit
GCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTGC	577	0.11092996608657536	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9225297415351015E-4	0.0	0.0	0.08766735621400062	0.0
2	1.9225297415351015E-4	0.0	0.0	0.44737267085521815	0.0
3	1.9225297415351015E-4	0.0	0.0	0.6136714934980044	0.0
4	1.9225297415351015E-4	0.0	0.0	0.7622830425186677	0.0
5	1.9225297415351015E-4	0.0	0.0	1.1619769757838154	0.0
6	1.9225297415351015E-4	0.0	0.0	1.3665341402831501	0.0
7	1.9225297415351015E-4	0.0	0.0	1.6003137568538186	0.0
8	1.9225297415351015E-4	0.0	0.0	1.9488683989941324	0.0
9	1.9225297415351015E-4	0.0	0.0	2.0657582072794667	0.0
10	1.9225297415351015E-4	0.0	0.0	2.3214546629036352	0.0
11	1.9225297415351015E-4	0.0	0.0	2.7955504971661913	0.0
12	1.9225297415351015E-4	0.0	0.0	3.1383375500819	0.0
13	1.9225297415351015E-4	0.0	0.0	3.2840653044902606	0.0
14	1.9225297415351015E-4	0.0	0.0	3.3480855448833795	0.0
15	1.9225297415351015E-4	0.0	0.0	3.472665472134854	0.0
16	1.9225297415351015E-4	0.0	0.0	3.7443189246137636	0.0
17	1.9225297415351015E-4	0.0	0.0	4.032890638818182	0.0
18	1.9225297415351015E-4	0.0	0.0	4.308196897806009	0.0
19	1.9225297415351015E-4	0.0	0.0	4.509293508770581	0.0
20	1.9225297415351015E-4	0.0	0.0	4.698470435337635	0.0
21	3.845059483070203E-4	0.0	0.0	4.96877811699747	0.0
22	3.845059483070203E-4	0.0	0.0	5.222744295854257	0.0
23	3.845059483070203E-4	0.0	0.0	5.481324546090728	0.0
24	5.767589224605305E-4	0.0	0.0	5.694533094426971	0.0
25	5.767589224605305E-4	0.0	0.0	5.890438875089398	0.0
26	5.767589224605305E-4	0.0	0.0	6.066734852388167	0.0
27	5.767589224605305E-4	0.0	0.0	6.232264663134338	0.0
28	5.767589224605305E-4	0.0	0.0	6.41144443504541	0.0
29	5.767589224605305E-4	0.0	0.0	6.6036974091989205	0.0
30	5.767589224605305E-4	0.0	0.0	6.8115228742588645	0.0
31	5.767589224605305E-4	0.0	0.0	7.03088351776802	0.0
32	5.767589224605305E-4	0.0	0.0	7.231211116835977	0.0
33	5.767589224605305E-4	0.0	0.0	7.417119742842422	0.0
34	5.767589224605305E-4	0.0	0.0	7.6132177764790026	0.0
35	5.767589224605305E-4	0.0	0.0	7.829310119427547	0.0
36	5.767589224605305E-4	0.0	0.0	8.057129893799457	0.0
37	5.767589224605305E-4	0.0	0.0	8.27802856110184	0.0
38	5.767589224605305E-4	0.0	0.0	8.500272999223299	0.0
39	5.767589224605305E-4	0.0	0.0	8.707137199412475	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGAC	20	7.0297194E-4	45.000004	26
TTACGAG	20	7.0297194E-4	45.000004	1
TCAAGCG	20	7.0297194E-4	45.000004	17
CCGTTAA	20	7.0297194E-4	45.000004	30
AACACGT	20	7.0297194E-4	45.000004	41
TTGTGCG	25	3.8878512E-5	44.999996	1
TCGTAGG	25	3.8878512E-5	44.999996	2
TGTAGCG	25	3.8878512E-5	44.999996	1
CGTTTTT	23675	0.0	44.524815	1
GTTTTTT	25840	0.0	40.994583	2
TGCGAGG	100	0.0	40.499996	2
ACGGGAT	45	1.9248546E-8	40.000004	5
CGAGATA	85	0.0	39.705883	19
TGCGGGT	70	0.0	38.571426	4
TAGTAGG	65	9.094947E-12	38.07692	2
AACGGGA	95	0.0	37.894737	4
GCGTTAG	30	1.1390629E-4	37.499996	1
CGTTAGG	60	1.546141E-10	37.499996	2
GTGTACG	30	1.1390629E-4	37.499996	1
CCGCCCA	60	1.546141E-10	37.499996	36
>>END_MODULE