##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550282_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	786896
Sequences flagged as poor quality	0
Sequence length	51
%GC	32
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.353356733291314	33.0	31.0	34.0	31.0	34.0
2	32.45611237063093	34.0	31.0	34.0	31.0	34.0
3	32.395114475102176	34.0	31.0	34.0	31.0	34.0
4	36.01613046705028	37.0	35.0	37.0	35.0	37.0
5	36.07218997173705	37.0	35.0	37.0	35.0	37.0
6	36.023169516683275	37.0	35.0	37.0	35.0	37.0
7	36.24147409568736	37.0	37.0	37.0	35.0	37.0
8	36.10001957056587	37.0	37.0	37.0	35.0	37.0
9	38.028260405441124	39.0	39.0	39.0	35.0	39.0
10	37.63594680872695	39.0	38.0	39.0	35.0	39.0
11	37.61795078383929	39.0	37.0	39.0	35.0	39.0
12	37.612664189422745	39.0	37.0	39.0	35.0	39.0
13	37.565739309896095	39.0	37.0	39.0	35.0	39.0
14	38.929440485146706	41.0	38.0	41.0	35.0	41.0
15	39.03168652528415	41.0	39.0	41.0	35.0	41.0
16	38.95425825013725	41.0	38.0	41.0	35.0	41.0
17	38.91654043228076	41.0	38.0	41.0	35.0	41.0
18	38.15154988715154	39.0	38.0	40.0	35.0	41.0
19	37.30361953803298	37.0	37.0	40.0	34.0	41.0
20	36.56899768203168	35.0	35.0	40.0	34.0	41.0
21	36.451270561802325	35.0	35.0	40.0	33.0	41.0
22	36.49242593684553	35.0	35.0	40.0	33.0	41.0
23	36.48783956202598	35.0	35.0	40.0	33.0	41.0
24	36.336027886785544	35.0	35.0	40.0	33.0	41.0
25	36.11222957036254	35.0	35.0	40.0	33.0	41.0
26	36.15414616416909	35.0	35.0	40.0	33.0	41.0
27	36.12025985644863	35.0	35.0	40.0	33.0	41.0
28	35.914394024115005	35.0	35.0	40.0	33.0	41.0
29	35.67503837864216	35.0	35.0	40.0	33.0	41.0
30	35.15713131087208	35.0	35.0	40.0	31.0	41.0
31	34.48090599011814	35.0	35.0	40.0	23.0	41.0
32	33.398159350155545	35.0	33.0	40.0	18.0	41.0
33	31.981855289644376	35.0	31.0	40.0	12.0	41.0
34	30.788320184624144	35.0	23.0	40.0	7.0	41.0
35	30.022179551046136	35.0	21.0	40.0	7.0	41.0
36	29.506556139566094	35.0	18.0	40.0	7.0	41.0
37	29.19722682540005	35.0	18.0	40.0	7.0	41.0
38	28.962819737296925	35.0	18.0	40.0	7.0	41.0
39	28.748192899697038	35.0	17.0	40.0	7.0	41.0
40	28.549280972326713	35.0	16.0	40.0	7.0	41.0
41	28.30582186210122	35.0	15.0	40.0	7.0	41.0
42	28.145893485289033	35.0	15.0	40.0	7.0	41.0
43	28.005323448079544	35.0	15.0	40.0	7.0	41.0
44	27.8259248998597	35.0	15.0	40.0	7.0	41.0
45	27.867812264899047	35.0	15.0	40.0	7.0	41.0
46	27.808526158475836	35.0	15.0	40.0	7.0	41.0
47	27.834377096846342	35.0	15.0	39.0	7.0	41.0
48	27.8133438218011	35.0	15.0	39.0	7.0	41.0
49	27.75297243854334	34.0	15.0	39.0	7.0	41.0
50	27.686133872837072	34.0	15.0	39.0	7.0	41.0
51	27.077579502246802	33.0	15.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	4.0
10	5.0
11	6.0
12	11.0
13	14.0
14	27.0
15	51.0
16	148.0
17	335.0
18	621.0
19	1108.0
20	1849.0
21	2798.0
22	4289.0
23	6958.0
24	12745.0
25	25615.0
26	45822.0
27	59382.0
28	55317.0
29	42452.0
30	32296.0
31	26934.0
32	23462.0
33	24109.0
34	36823.0
35	47302.0
36	48885.0
37	51684.0
38	91822.0
39	143965.0
40	54.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.957798743417175	12.094355543807568	13.274308167788373	51.67353754498688
2	62.479921107744865	13.445614159939815	14.14532543055245	9.929139301762877
3	19.118917874789044	13.138203777881701	57.80636831296639	9.936510034362863
4	17.005042597751164	14.440281816148511	57.22890953823631	11.325766047864013
5	15.168840609178341	17.329100668957526	56.58409751733393	10.917961204530204
6	15.733464142656716	19.777963034505195	55.706217848356076	8.782354974482015
7	46.39596592179907	3.8086354486488685	47.251606311380414	2.543792318171652
8	46.986768264167054	3.4920751916390476	47.113849860718574	2.407306683475326
9	43.22324678229397	5.258763546898193	48.248434354730485	3.2695553160773465
10	25.233703056058232	14.556180232203495	52.29560196010654	7.914514751631728
11	18.917239381061794	15.770571968849758	55.26537178991887	10.046816860169578
12	17.91215103393587	13.884935239218397	57.30566682255343	10.897246904292308
13	14.639672841137838	14.701307415465322	58.024059087859136	12.634960655537707
14	11.760766352859845	15.962211016449443	58.6172759805616	13.659746650129115
15	11.748566519590899	16.268223500945485	60.37977064313454	11.603439336329071
16	13.55617514893963	15.541825095057035	59.718438014680466	11.183561741322869
17	13.451586994977735	16.22158455501108	58.01516337610053	12.311665073910657
18	13.804492588601288	16.579446330900144	57.73316931335272	11.88289176714585
19	14.85901567678575	16.264919379435145	57.09140725076757	11.784657693011528
20	16.28016917102133	15.716435208718815	57.766337610052666	10.237058010207194
21	15.520856631626035	15.291474349850553	58.01389256013502	11.173776458388401
22	15.081662633944005	14.65263516398609	58.02749029096602	12.238211911103882
23	14.525807730627683	15.769174071287692	58.25001016652772	11.4550080315569
24	13.79025944978752	14.839445110916818	59.15635611313312	12.213939326162542
25	13.463532665053576	16.290589861938553	57.59426912832192	12.651608344685956
26	13.462770175474267	17.548316423008885	57.171468656595025	11.817444744921819
27	13.550710640287916	17.21205851853358	57.748037860149246	11.489192981029259
28	12.559092942396454	17.47575283137797	57.94171021329375	12.023444012931824
29	13.40138976433989	16.87465687968931	57.1644791687847	12.55947418718611
30	13.445359996746712	16.52543665236575	56.52754620686851	13.501657144019033
31	14.397074073320997	17.943667269880645	54.661225879912976	12.998032776885381
32	15.163503182123177	19.05029381265123	52.81473028201948	12.971472723206116
33	15.076833533275044	21.297223521278543	50.03151623594477	13.594426709501636
34	15.129953640633579	22.552535532014396	47.9983377727171	14.31917305463492
35	16.660524389500008	23.105467558610034	44.52303735182286	15.710970700067097
36	16.535603180089872	25.980815762184584	42.39569142555052	15.087889632175028
37	17.58212012769159	26.279838758870294	41.87249649254793	14.26554462089018
38	17.273057684878307	26.01881315955349	40.886216221711635	15.821912933856572
39	17.85509139708424	25.774562330981475	40.577916268477665	15.79243000345662
40	18.092225656249365	25.435508631382035	40.917859539252966	15.554406173115634
41	16.236580183404158	26.611775889062848	40.67729407698095	16.47434985055204
42	17.31779040686444	27.392056891889144	38.677538073646325	16.612614627600088
43	17.723180699863768	26.998485187369102	38.970588235294116	16.307745877473007
44	17.011777922368392	26.921982066245093	39.065136943128444	17.001103068258068
45	17.182448506537078	25.606433378743827	39.665978731624	17.545139383095098
46	16.616172912303533	26.08807262967406	40.05395884589577	17.241795612126634
47	16.919643764868546	25.265346373599563	41.619985360200076	16.195024501331815
48	16.97047640348915	24.198623452146155	42.07201459913381	16.758885545230882
49	16.336847563083303	24.493325674549116	42.181177690571566	16.98864907179602
50	15.428849555722737	23.9002358634432	42.80502124804295	17.86589333279112
51	15.584905756287998	23.36140989406478	42.25691832211626	18.796766027530957
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	235.0
1	748.0
2	1261.0
3	52927.5
4	104594.0
5	71959.0
6	39324.0
7	37349.0
8	35374.0
9	33559.5
10	31745.0
11	29985.5
12	28226.0
13	26368.5
14	24511.0
15	22255.5
16	20000.0
17	18341.5
18	16683.0
19	15272.0
20	13861.0
21	12481.5
22	11102.0
23	10215.5
24	9329.0
25	9029.5
26	8459.0
27	8188.0
28	8948.0
29	9708.0
30	10454.5
31	11201.0
32	12060.0
33	12919.0
34	14125.5
35	15332.0
36	16051.5
37	16771.0
38	18169.0
39	19567.0
40	21078.5
41	22590.0
42	23541.0
43	24492.0
44	26460.0
45	28428.0
46	29261.5
47	30095.0
48	31143.5
49	32192.0
50	31594.0
51	30996.0
52	29521.0
53	28046.0
54	26589.5
55	25133.0
56	24210.5
57	23288.0
58	23129.5
59	22971.0
60	22655.0
61	22339.0
62	20405.0
63	18471.0
64	16005.5
65	13540.0
66	11284.5
67	9029.0
68	7356.0
69	5683.0
70	4806.0
71	3929.0
72	3454.0
73	2979.0
74	2320.0
75	1424.5
76	1188.0
77	886.5
78	585.0
79	468.5
80	352.0
81	243.5
82	135.0
83	104.0
84	73.0
85	46.5
86	20.0
87	16.0
88	12.0
89	8.0
90	4.0
91	3.0
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	786896.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.687735664564
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.35689451255787	30.661571348790993
2	6.438664392257882	4.853173632965115
3	2.3157089486238376	2.618214801954019
4	1.2792027369798686	1.9284101845073638
5	0.8612324727347482	1.6228950889083007
6	0.7082486742679839	1.60153732923538
7	0.6008901343489836	1.5852331982752197
8	0.5230896232211855	1.5771250759069135
9	0.47731713699120104	1.6190101878381786
>10	5.284616374597503	36.70573631279992
>50	0.08078566709928271	2.1335332111165535
>100	0.06929314237580242	4.772678472625408
>500	0.002704122629299593	0.7172075217104223
>1k	0.0010140459859873474	0.4219167516705007
>5k	0.0	0.0
>10k+	3.3801532866244914E-4	7.181756881695697
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	56376	7.164352087188142	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1154	0.14665216242044693	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	1079	0.1371210426790834	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	1079	0.1371210426790834	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA	922	0.11716923202049571	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAA	888	0.11284845773774424	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.541631931030276E-4	0.0	0.0	0.047401435513714646	0.0
2	2.541631931030276E-4	0.0	0.0	0.24209044143063377	0.0
3	2.541631931030276E-4	0.0	0.0	0.3297767430511783	0.0
4	2.541631931030276E-4	0.0	0.0	0.4266129196234318	0.0
5	2.541631931030276E-4	0.0	0.0	0.6786157255850837	0.0
6	2.541631931030276E-4	0.0	0.0	0.8255220511986336	0.0
7	2.541631931030276E-4	0.0	0.0	0.9659472153880564	0.0
8	2.541631931030276E-4	0.0	0.0	1.1727089729773692	0.0
9	2.541631931030276E-4	0.0	0.0	1.243874667046217	0.0
10	2.541631931030276E-4	0.0	0.0	1.409589068949391	0.0
11	2.541631931030276E-4	0.0	0.0	1.6915831316972	0.0
12	2.541631931030276E-4	0.0	0.0	1.9040635611313312	0.0
13	2.541631931030276E-4	0.0	0.0	1.988826986031191	0.0
14	2.541631931030276E-4	0.0	0.0	2.02568064903113	0.0
15	2.541631931030276E-4	0.0	0.0	2.1100628291413352	0.0
16	5.083263862060552E-4	0.0	0.0	2.2811146580996726	0.0
17	5.083263862060552E-4	0.0	0.0	2.4578851589028283	0.0
18	5.083263862060552E-4	0.0	0.0	2.635545230881845	0.0
19	5.083263862060552E-4	0.0	0.0	2.762245582643704	0.0
20	6.35407982757569E-4	0.0	0.0	2.893902116671072	0.0
21	6.35407982757569E-4	0.0	0.0	3.065462272015616	0.0
22	6.35407982757569E-4	0.0	0.0	3.252526382139444	0.0
23	6.35407982757569E-4	0.0	0.0	3.4190032736219274	0.0
24	7.624895793090828E-4	0.0	0.0	3.55942843781135	0.0
25	7.624895793090828E-4	0.0	0.0	3.6926099509973365	0.0
26	7.624895793090828E-4	0.0	0.0	3.8101604278074865	0.0
27	7.624895793090828E-4	0.0	0.0	3.9386399219210673	0.0
28	7.624895793090828E-4	0.0	0.0	4.066865252841544	0.0
29	7.624895793090828E-4	0.0	0.0	4.197123889306846	0.0
30	7.624895793090828E-4	0.0	0.0	4.350638457941074	0.0
31	7.624895793090828E-4	0.0	0.0	4.48877615339257	0.0
32	7.624895793090828E-4	0.0	0.0	4.635428315813018	0.0
33	7.624895793090828E-4	0.0	0.0	4.770261889754173	0.0
34	8.895711758605966E-4	0.0	0.0	4.913609930664281	0.0
35	8.895711758605966E-4	0.0	0.0	5.0601350114881765	0.0
36	0.0010166527724121105	0.0	0.0	5.217080783229296	0.0
37	0.0010166527724121105	0.0	0.0	5.3717390862324885	0.0
38	0.0010166527724121105	0.0	0.0	5.516993351090869	0.0
39	0.0010166527724121105	0.0	0.0	5.669745430145788	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGGCG	30	2.1646247E-6	45.000004	1
CTACTCC	30	2.1646247E-6	45.000004	24
GCGATAT	20	7.031927E-4	45.000004	9
TATAGCG	30	2.1646247E-6	45.000004	1
ACCGTTG	20	7.031927E-4	45.000004	16
TACGGGT	25	3.8896804E-5	45.0	4
ACGGGTA	25	3.8896804E-5	45.0	5
CGTTTTT	33965	0.0	44.516415	1
GGCGATA	95	0.0	42.63158	8
GTTTTTT	37380	0.0	40.76846	2
TAACGTC	115	0.0	39.130432	32
TCGTACG	70	0.0	38.571426	30
GACCGAT	135	0.0	38.333332	9
ACGTGCG	30	1.1395945E-4	37.500004	1
ATAACGT	120	0.0	37.500004	31
CACAACG	60	1.546141E-10	37.500004	12
AATAACG	120	0.0	37.500004	30
CGAATAT	55	2.746674E-9	36.81818	14
TAACGGG	135	0.0	36.666664	3
CTCGTAC	75	1.8189894E-12	36.0	29
>>END_MODULE