FastQCFastQC Report
Sat 18 Jun 2016
SRR3550281_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550281_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences389208
Sequences flagged as poor quality0
Sequence length51
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT304477.822809397545785No Hit
GCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC11100.28519454893013507No Hit
CCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC10930.28082670448706093No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA6520.16751968099319645No Hit
CTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGCT5780.14850671106452076No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA5650.14516659472569937No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA5590.14362500256932026No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA5250.13488931368317197No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC4100.10534213068590574No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAA3990.10251587839921071No Hit
TCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC3920.10071735421676842No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGG351.209155E-745.0000042
TTGATCA207.027532E-445.013
AGGTAAG207.027532E-445.01
ATTCGGA207.027532E-445.025
ATGTCCA207.027532E-445.027
CTACGGG253.886035E-545.03
TACGAGG253.886035E-545.02
TGTTACG207.027532E-445.01
GCGATCG253.886035E-545.09
ATTGGTC207.027532E-445.038
ACGTAAG207.027532E-445.01
GACGAGG253.886035E-545.02
CGTCCTT207.027532E-445.026
TTACCGG207.027532E-445.02
TTCGAGT253.886035E-545.015
GTAGTAA207.027532E-445.09
CGAAGGC207.027532E-445.032
TAGGGAC650.044.9999965
CGTTTTT167750.044.624441
GTTTTTT182050.041.279872