##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550281_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	389208
Sequences flagged as poor quality	0
Sequence length	51
%GC	32
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14890495570492	33.0	31.0	34.0	30.0	34.0
2	32.27048262111776	34.0	31.0	34.0	30.0	34.0
3	32.20811237179092	34.0	31.0	34.0	30.0	34.0
4	35.884542455447985	37.0	35.0	37.0	35.0	37.0
5	35.95012949374114	37.0	35.0	37.0	35.0	37.0
6	35.908565599884895	37.0	35.0	37.0	35.0	37.0
7	36.14661569135269	37.0	35.0	37.0	35.0	37.0
8	35.98309130336478	37.0	36.0	37.0	35.0	37.0
9	37.90343209800415	39.0	38.0	39.0	35.0	39.0
10	37.48427062136441	39.0	37.0	39.0	34.0	39.0
11	37.49975334525498	39.0	37.0	39.0	35.0	39.0
12	37.523357690489405	39.0	37.0	39.0	35.0	39.0
13	37.477464491994	39.0	37.0	39.0	35.0	39.0
14	38.75749984584078	40.0	38.0	41.0	35.0	41.0
15	38.8675207087213	40.0	38.0	41.0	35.0	41.0
16	38.78564161065548	40.0	38.0	41.0	34.0	41.0
17	38.739902571375715	40.0	38.0	41.0	34.0	41.0
18	37.99460185813241	39.0	38.0	40.0	34.0	41.0
19	37.17804618610101	37.0	37.0	40.0	34.0	41.0
20	36.462318349057576	35.0	35.0	40.0	34.0	41.0
21	36.36277260487965	35.0	35.0	40.0	33.0	41.0
22	36.4007600049331	35.0	35.0	40.0	33.0	41.0
23	36.40415921563791	35.0	35.0	40.0	33.0	41.0
24	36.2722939919015	35.0	35.0	40.0	33.0	41.0
25	36.0312352264085	35.0	35.0	40.0	33.0	41.0
26	36.08921964605044	35.0	35.0	40.0	33.0	41.0
27	36.06078754804629	35.0	35.0	40.0	33.0	41.0
28	35.855262481757826	35.0	35.0	40.0	33.0	41.0
29	35.66131220324351	35.0	35.0	40.0	32.0	41.0
30	35.15676450638219	35.0	35.0	40.0	31.0	41.0
31	34.506413023370534	35.0	35.0	40.0	24.0	41.0
32	33.473759532178164	35.0	33.0	40.0	18.0	41.0
33	32.127995827423895	35.0	32.0	40.0	12.0	41.0
34	30.990555178721916	35.0	25.0	40.0	8.0	41.0
35	30.238874843271464	35.0	22.0	40.0	7.0	41.0
36	29.75578097058642	35.0	20.0	40.0	7.0	41.0
37	29.44102639205772	35.0	18.0	40.0	7.0	41.0
38	29.21091550019527	35.0	18.0	40.0	7.0	41.0
39	29.008476187539824	35.0	18.0	40.0	7.0	41.0
40	28.805214692400977	35.0	18.0	40.0	7.0	41.0
41	28.572431707467473	35.0	17.0	40.0	7.0	41.0
42	28.4407077963454	35.0	15.0	40.0	7.0	41.0
43	28.29460339972457	35.0	15.0	40.0	7.0	41.0
44	28.086683726953197	35.0	15.0	40.0	7.0	41.0
45	28.112204785102055	35.0	15.0	40.0	7.0	41.0
46	28.02810065569053	35.0	15.0	39.0	7.0	41.0
47	28.01560348194282	35.0	15.0	39.0	7.0	41.0
48	27.986955561036773	35.0	15.0	39.0	7.0	41.0
49	27.93266582392962	35.0	15.0	39.0	7.0	41.0
50	27.85779326221455	34.0	15.0	39.0	7.0	41.0
51	27.17913043925099	33.0	15.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	6.0
12	6.0
13	6.0
14	13.0
15	25.0
16	58.0
17	111.0
18	310.0
19	542.0
20	906.0
21	1352.0
22	2141.0
23	3599.0
24	6327.0
25	12737.0
26	22186.0
27	28146.0
28	25821.0
29	20178.0
30	15957.0
31	13289.0
32	12027.0
33	12903.0
34	18928.0
35	25126.0
36	27219.0
37	27854.0
38	48993.0
39	62413.0
40	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.3397052475797	12.071180448500545	13.087603543606505	51.50151076031325
2	61.36641590100923	13.812665721156812	13.993802799531355	10.827115578302603
3	18.807167375799057	13.734044521181477	57.275287250005135	10.183500853014326
4	17.44157365727323	14.914904112967871	56.575404411009025	11.068117818749872
5	14.579093954903291	17.066966763273108	56.798678341657926	11.55526094016567
6	15.200098661898009	19.63089145135763	55.631693079278946	9.537316807465416
7	45.37188341452386	4.130182318965694	47.34897535508006	3.148958911430392
8	45.57563051119196	3.611950422396251	47.175032373435286	3.637386692976506
9	41.64457051242523	5.210838420587449	48.81991120429179	4.324679862695525
10	23.741290004316458	14.500986618980082	53.330609853857055	8.427113522846396
11	17.904822100265154	15.791042321843332	55.302563153892	11.001572423999505
12	16.64611210458161	14.221958438675465	57.56921748782142	11.562711968921501
13	14.200890012538284	15.596030914061377	58.082310743869606	12.12076832953074
14	11.548066843435901	16.702894082341576	58.104149966084975	13.644889108137551
15	10.978191609627757	16.1206861112824	60.87336334299398	12.027758936095868
16	13.095054572362336	15.411296787321946	59.207673018026355	12.285975622289367
17	12.438850177796962	16.511736654950564	57.29481408398595	13.75459908326653
18	12.6032866744774	16.501973237960165	58.64447801689585	12.250262070666585
19	14.007420196912705	16.270477482477236	57.11624632587202	12.605855994738032
20	14.879447493371153	15.954708022445582	58.17943104972149	10.98641343446178
21	14.582434071242112	15.8254712133358	57.610840476043656	11.98125423937843
22	12.773889539783356	15.329592403033853	57.737251032866745	14.159267024316048
23	12.91237590183141	15.880197734887258	58.05764526936754	13.149781093913795
24	13.374596616719082	14.980935643666113	58.43148136728947	13.21298637232534
25	12.57168403527163	16.2804978314937	57.41993998067871	13.727878152555958
26	12.086596370064335	17.053606297917824	57.447688639493535	13.412108692524304
27	12.593780189513062	16.745287866642002	57.51808801463485	13.142843929210088
28	10.976650017471377	16.5805944379355	58.24469178434154	14.19806376025159
29	11.781104191075208	15.449579659205362	57.891153316478594	14.878162833240838
30	12.235103081128857	15.921306859057369	56.14632792748351	15.69726213233027
31	12.940895356724425	18.240632258329736	53.809531150438836	15.008941234506997
32	13.23816571087953	19.38577829849335	52.60811699656739	14.767938994059731
33	13.45039156440772	20.85491562352264	49.35998232307661	16.334710488993032
34	13.168023267764282	22.44095702041068	47.76931614971943	16.62170356210561
35	14.581149411111797	22.580984974615113	44.7832521428131	18.05461347145999
36	15.399734846149101	25.15415921563791	42.3259542455448	17.120151692668188
37	15.578045672236954	25.495364946249822	42.768134262399535	16.158455119113686
38	15.175433187395942	25.717868080820537	41.85294238556247	17.253756346221046
39	16.50377176214261	24.816036669338757	41.77432118558714	16.905870382931493
40	16.905356518879366	25.12178578035395	41.35475118702596	16.618106513740724
41	15.019732379601653	26.389745328975767	39.903856035847156	18.686666255575425
42	15.8352346303262	25.979681815378925	39.79774310908306	18.387340445211812
43	17.05180777373538	24.662391317752974	40.30775318081849	17.978047727693163
44	15.775626400279542	25.111765431337485	39.82549176789789	19.28711640048509
45	15.811853815954452	24.380023021109533	39.722718957472615	20.0854042054634
46	15.913341966249408	24.679862695525273	40.53256870362377	18.87422663460155
47	15.199584797845883	24.228176193706194	42.3357176625352	18.236521345912728
48	14.972713818832089	23.001839633306613	42.65251485067111	19.37293169719019
49	15.193675361246427	22.62671887525436	43.50501531314875	18.674590450350458
50	14.784639575753838	22.185566586503874	43.472128013812664	19.55766582392962
51	14.186244887052682	22.128270744691783	41.62324515426199	22.062239213993546
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	111.0
1	389.0
2	667.0
3	26990.0
4	53313.0
5	35867.0
6	18421.0
7	17465.0
8	16509.0
9	15661.5
10	14814.0
11	14041.0
12	13268.0
13	12418.0
14	11568.0
15	10604.5
16	9641.0
17	8647.5
18	7654.0
19	6862.5
20	6071.0
21	5478.0
22	4885.0
23	4585.0
24	4285.0
25	4151.5
26	3975.5
27	3933.0
28	4194.0
29	4455.0
30	4798.5
31	5142.0
32	5414.5
33	5687.0
34	6121.5
35	6556.0
36	7144.0
37	7732.0
38	7852.5
39	7973.0
40	9197.0
41	10421.0
42	11633.0
43	12845.0
44	14324.5
45	15804.0
46	17907.5
47	20011.0
48	21465.5
49	22920.0
50	22591.5
51	22263.0
52	18955.0
53	15647.0
54	13997.0
55	12347.0
56	11139.5
57	9932.0
58	9445.5
59	8959.0
60	8652.0
61	8345.0
62	7856.0
63	7367.0
64	6370.0
65	5373.0
66	4323.0
67	3273.0
68	2851.0
69	2429.0
70	2030.0
71	1631.0
72	1324.5
73	1018.0
74	934.5
75	661.5
76	472.0
77	372.5
78	273.0
79	230.5
80	188.0
81	147.0
82	106.0
83	62.5
84	19.0
85	11.0
86	3.0
87	3.5
88	4.0
89	3.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	389208.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.97744244208271
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.67131681287644	35.10024317494499
2	7.513079403633753	6.457858752649336
3	2.4832180081546205	3.201670770498255
4	1.2127780692716092	2.0848839866856315
5	0.8482835027229414	1.8228527706421762
6	0.6476170239604564	1.6699754023064048
7	0.517124475362654	1.555728116270348
8	0.4624009653178422	1.5898248697688837
9	0.4115217540023512	1.5917537246670874
>10	4.078877816961674	29.60094814998265
>50	0.10591223225371875	3.1559402268137084
>100	0.043082941933716104	3.031471919302476
>500	0.0029918709676191742	0.7403806969521403
>1k	0.0011967483870476696	0.5665365319157919
>5k	0.0	0.0
>10k+	5.983741935238348E-4	7.8299309066001435
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30447	7.822809397545785	No Hit
GCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC	1110	0.28519454893013507	No Hit
CCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC	1093	0.28082670448706093	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	652	0.16751968099319645	No Hit
CTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGCT	578	0.14850671106452076	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	565	0.14516659472569937	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	559	0.14362500256932026	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA	525	0.13488931368317197	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	410	0.10534213068590574	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAA	399	0.10251587839921071	No Hit
TCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC	392	0.10071735421676842	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.5693202606318473E-4	0.0	0.0	0.16135331236768	0.0
2	2.5693202606318473E-4	0.0	0.0	0.9588703212678054	0.0
3	2.5693202606318473E-4	0.0	0.0	1.2900557028632504	0.0
4	2.5693202606318473E-4	0.0	0.0	1.6006865223736408	0.0
5	2.5693202606318473E-4	0.0	0.0	2.4390557234178125	0.0
6	2.5693202606318473E-4	0.0	0.0	2.8840619925592486	0.0
7	2.5693202606318473E-4	0.0	0.0	3.359129308750077	0.0
8	2.5693202606318473E-4	0.0	0.0	4.057470555589813	0.0
9	2.5693202606318473E-4	0.0	0.0	4.267640952909498	0.0
10	2.5693202606318473E-4	0.0	0.0	4.77842182072311	0.0
11	2.5693202606318473E-4	0.0	0.0	5.5957225956301	0.0
12	2.5693202606318473E-4	0.0	0.0	6.261690407185875	0.0
13	2.5693202606318473E-4	0.0	0.0	6.519136297301186	0.0
14	2.5693202606318473E-4	0.0	0.0	6.624478427987092	0.0
15	2.5693202606318473E-4	0.0	0.0	6.864709872356169	0.0
16	2.5693202606318473E-4	0.0	0.0	7.309459209471542	0.0
17	2.5693202606318473E-4	0.0	0.0	7.8192123491809005	0.0
18	2.5693202606318473E-4	0.0	0.0	8.385233602598097	0.0
19	2.5693202606318473E-4	0.0	0.0	8.701516926681878	0.0
20	2.5693202606318473E-4	0.0	0.0	9.01728638671353	0.0
21	2.5693202606318473E-4	0.0	0.0	9.443022753900228	0.0
22	2.5693202606318473E-4	0.0	0.0	9.879550266181578	0.0
23	2.5693202606318473E-4	0.0	0.0	10.31530698238474	0.0
24	2.5693202606318473E-4	0.0	0.0	10.650860208423259	0.0
25	2.5693202606318473E-4	0.0	0.0	10.985385706357526	0.0
26	2.5693202606318473E-4	0.0	0.0	11.26235843045364	0.0
27	2.5693202606318473E-4	0.0	0.0	11.524686029064151	0.0
28	2.5693202606318473E-4	0.0	0.0	11.818102402828307	0.0
29	2.5693202606318473E-4	0.0	0.0	12.106123204045138	0.0
30	2.5693202606318473E-4	0.0	0.0	12.440391769953342	0.0
31	2.5693202606318473E-4	0.0	0.0	12.737662124108446	0.0
32	2.5693202606318473E-4	0.0	0.0	13.040071118784814	0.0
33	2.5693202606318473E-4	0.0	0.0	13.319099299089434	0.0
34	2.5693202606318473E-4	0.0	0.0	13.6068631682802	0.0
35	2.5693202606318473E-4	0.0	0.0	13.93599309366714	0.0
36	2.5693202606318473E-4	0.0	0.0	14.253304145855173	0.0
37	2.5693202606318473E-4	0.0	0.0	14.561365645104932	0.0
38	2.5693202606318473E-4	0.0	0.0	14.831401204497338	0.0
39	2.5693202606318473E-4	0.0	0.0	15.102978356046124	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGG	35	1.209155E-7	45.000004	2
TTGATCA	20	7.027532E-4	45.0	13
AGGTAAG	20	7.027532E-4	45.0	1
ATTCGGA	20	7.027532E-4	45.0	25
ATGTCCA	20	7.027532E-4	45.0	27
CTACGGG	25	3.886035E-5	45.0	3
TACGAGG	25	3.886035E-5	45.0	2
TGTTACG	20	7.027532E-4	45.0	1
GCGATCG	25	3.886035E-5	45.0	9
ATTGGTC	20	7.027532E-4	45.0	38
ACGTAAG	20	7.027532E-4	45.0	1
GACGAGG	25	3.886035E-5	45.0	2
CGTCCTT	20	7.027532E-4	45.0	26
TTACCGG	20	7.027532E-4	45.0	2
TTCGAGT	25	3.886035E-5	45.0	15
GTAGTAA	20	7.027532E-4	45.0	9
CGAAGGC	20	7.027532E-4	45.0	32
TAGGGAC	65	0.0	44.999996	5
CGTTTTT	16775	0.0	44.62444	1
GTTTTTT	18205	0.0	41.27987	2
>>END_MODULE