SRR3550280_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550280_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	566536
Sequences flagged as poor quality	0
Sequence length	51
%GC	31

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	39450	6.9633703771693245	No Hit
CCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	979	0.17280455257918298	RNA PCR Primer, Index 41 (96% over 26bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	925	0.16327294293743028	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	832	0.1468573929988562	No Hit
GCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	814	0.14368018978493863	Illumina PCR Primer Index 11 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	805	0.14209158817797987	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA	654	0.11543838343900477	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAA	595	0.10502421734894164	No Hit
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCT	590	0.1041416609006312	RNA PCR Primer, Index 41 (96% over 27bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGTAG	25	3.888292E-5	45.0	1
GCGTTAG	20	7.0302555E-4	45.0	1
TACGCGG	20	7.0302555E-4	45.0	2
TACGAGA	25	3.888292E-5	45.0	29
TCGTAAG	20	7.0302555E-4	45.0	1
CGTAACG	20	7.0302555E-4	45.0	1
CGTAAAT	20	7.0302555E-4	45.0	14
AAGGGCG	85	0.0	45.0	5
TGTACGG	20	7.0302555E-4	45.0	2
TGCGTTG	20	7.0302555E-4	45.0	1
CGTTTTT	24600	0.0	44.780487	1
CGGGTAT	60	3.6379788E-12	41.249996	6
TGCGCGG	60	3.6379788E-12	41.249996	2
GTTTTTT	26900	0.0	41.06877	2
GCTACGA	55	6.002665E-11	40.909092	10
ATAGCGG	50	1.0786607E-9	40.5	2
TGCGAAG	50	1.0786607E-9	40.5	1
CACATCC	80	0.0	39.375	45
GTAGCGG	40	3.454079E-7	39.375	2
TCGTAGG	40	3.454079E-7	39.375	2