##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550280_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	566536
Sequences flagged as poor quality	0
Sequence length	51
%GC	31
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.25972577206038	33.0	31.0	34.0	30.0	34.0
2	32.37795303387605	34.0	31.0	34.0	31.0	34.0
3	32.317496152053884	34.0	31.0	34.0	30.0	34.0
4	35.955865117132895	37.0	35.0	37.0	35.0	37.0
5	36.029087295423416	37.0	35.0	37.0	35.0	37.0
6	35.992259979948315	37.0	35.0	37.0	35.0	37.0
7	36.23483061976644	37.0	37.0	37.0	35.0	37.0
8	36.09987008769081	37.0	37.0	37.0	35.0	37.0
9	38.05149010830733	39.0	39.0	39.0	35.0	39.0
10	37.63133499018597	39.0	38.0	39.0	35.0	39.0
11	37.59732656000678	39.0	37.0	39.0	35.0	39.0
12	37.64889962862025	39.0	37.0	39.0	35.0	39.0
13	37.63023002951269	39.0	38.0	39.0	35.0	39.0
14	38.939666676080606	41.0	38.0	41.0	35.0	41.0
15	39.05531157772851	41.0	39.0	41.0	35.0	41.0
16	38.95367461202819	41.0	38.0	41.0	35.0	41.0
17	38.90327357837808	41.0	38.0	41.0	35.0	41.0
18	38.13337369558157	39.0	38.0	40.0	35.0	41.0
19	37.29721853509751	37.0	37.0	40.0	34.0	41.0
20	36.57254967027691	35.0	35.0	40.0	34.0	41.0
21	36.46342862589491	35.0	35.0	40.0	33.0	41.0
22	36.524155569990256	35.0	35.0	40.0	33.0	41.0
23	36.52962388974399	35.0	35.0	40.0	33.0	41.0
24	36.39171208890521	35.0	35.0	40.0	33.0	41.0
25	36.178359362864846	35.0	35.0	40.0	33.0	41.0
26	36.22377395258201	35.0	35.0	40.0	33.0	41.0
27	36.22052614485222	35.0	35.0	40.0	33.0	41.0
28	36.03300055071522	35.0	35.0	40.0	33.0	41.0
29	35.831407006792155	35.0	35.0	40.0	33.0	41.0
30	35.3436568902947	35.0	35.0	40.0	31.0	41.0
31	34.69765381193781	35.0	35.0	40.0	25.0	41.0
32	33.60756951014587	35.0	33.0	40.0	18.0	41.0
33	32.1190445090868	35.0	31.0	40.0	12.0	41.0
34	30.831730022452238	35.0	23.0	40.0	7.0	41.0
35	30.038991343886355	35.0	21.0	40.0	7.0	41.0
36	29.535244009206828	35.0	18.0	40.0	7.0	41.0
37	29.230435488653853	35.0	18.0	40.0	7.0	41.0
38	28.99750766058997	35.0	18.0	40.0	7.0	41.0
39	28.776166033579507	35.0	17.0	40.0	7.0	41.0
40	28.58381109055735	35.0	15.0	40.0	7.0	41.0
41	28.353896310208	35.0	15.0	40.0	7.0	41.0
42	28.21981833458068	35.0	15.0	40.0	7.0	41.0
43	28.051562477936088	35.0	15.0	40.0	7.0	41.0
44	27.8524877501165	35.0	14.0	40.0	7.0	41.0
45	27.842866119717016	35.0	14.0	40.0	7.0	41.0
46	27.749452815002048	35.0	13.0	40.0	7.0	41.0
47	27.76033297089682	35.0	15.0	40.0	7.0	41.0
48	27.71752015758928	35.0	15.0	40.0	7.0	41.0
49	27.678414081364643	35.0	15.0	39.0	7.0	41.0
50	27.58229309346626	34.0	13.0	39.0	7.0	41.0
51	26.890684793199373	33.0	12.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	4.0
10	2.0
11	8.0
12	3.0
13	4.0
14	11.0
15	29.0
16	65.0
17	178.0
18	385.0
19	729.0
20	1222.0
21	1855.0
22	2917.0
23	4944.0
24	9463.0
25	19355.0
26	34007.0
27	42309.0
28	38993.0
29	30322.0
30	23462.0
31	19419.0
32	16826.0
33	16927.0
34	25023.0
35	32858.0
36	34807.0
37	37343.0
38	70949.0
39	102087.0
40	28.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.102101190392137	12.708459833090924	14.817063699394215	51.37237527712273
2	61.80454551873138	13.82030444667241	14.365018286569608	10.010131748026604
3	18.384709886044313	13.521823855853821	58.5613270824802	9.532139175621673
4	16.51510230594349	14.58000903737803	57.89146673821257	11.013421918465905
5	14.452214863662679	17.189198921162998	57.373935636923335	10.984650578250985
6	14.916969089342954	19.185894629820524	56.926303006340284	8.970833274496236
7	46.6018046514255	3.331650592371888	47.50324780772978	2.563296948472824
8	47.68734908284734	2.7899374443989435	47.083856983492666	2.4388564892610534
9	44.21660759422173	4.343236793425308	48.30708022085092	3.1330753915020404
10	23.00206871231484	16.938552889842835	52.277878193089236	7.781500204753096
11	16.346534024316195	15.372191705381477	57.76596721126283	10.515307059039495
12	15.91125718400949	13.768763150091079	58.888579013513706	11.431400652385728
13	13.64608780377593	14.247814790233985	60.034313794710314	12.071783611279779
14	11.262832370758433	16.334884279198498	59.67934959119985	12.722933758843217
15	11.476587542539221	15.600244291624893	61.67339763051245	11.249770535323439
16	13.263940861657511	15.412789302003757	60.034313794710314	11.288956041628422
17	13.070660999477527	15.661670220427299	58.614633491958145	12.65303528813703
18	13.150973636273777	15.526639083835803	59.53143313046303	11.790954149427398
19	13.543181721902933	15.956267562873322	58.44271149582727	12.057839219396472
20	14.759521018964373	16.016987446517078	58.61180931132355	10.611682223194997
21	14.395731250970812	15.665553468799864	58.987248824434815	10.951466455794513
22	13.55465495573097	14.42591468150303	59.31467726675799	12.70475309600802
23	12.821603569764322	15.140255870765495	59.570618636768	12.467521922702177
24	13.421741954615419	14.719982490080067	59.50019063219284	12.358084923111683
25	12.675981755793101	15.792994619935891	58.57174124857026	12.95928237570075
26	12.106026801474222	16.48562492056992	58.92370476015646	12.484643517799398
27	12.517827640255872	16.310878743804455	58.79396896225483	12.37732465368485
28	11.739765875425393	16.257748845616167	59.17735148340088	12.825133795557564
29	12.534066678904782	15.653903723682166	59.03826058714715	12.773769010265898
30	12.737584195885168	15.758574918451785	57.952010110566675	13.551830775096374
31	13.563833542793397	17.172077326065775	56.0416284225539	13.222460708586922
32	13.760467119476964	18.65812587373088	54.561404747447654	13.020002259344507
33	14.327597893161245	19.541564878489627	51.14396966829998	14.986867560049141
34	14.3422483302032	22.34738833895816	48.45499668158775	14.855366649250886
35	14.522995890817178	23.65922024372679	45.62728582120113	16.190498044254912
36	15.954502449976701	24.498707937359672	43.40783286499004	16.13895674767358
37	16.35112331784741	25.75882203425731	42.56075518590169	15.329299461993589
38	16.46762076902439	25.561305901125436	41.64430856997613	16.32676475987404
39	16.915606422186762	24.64044650295833	41.4233164353192	17.020630639535707
40	17.49103322648517	24.52218393888473	41.75215696795967	16.23462586667043
41	15.930320403292994	25.247468828106246	41.123247242893655	17.698963525707104
42	16.976855839699507	24.99594024033777	40.21174294307864	17.815460976884083
43	17.438609373455527	25.070604515864837	39.790940028524226	17.699846082155414
44	16.88559950294421	25.39238459691882	39.515229394072044	18.20678650606493
45	16.73115212448988	24.619971193357525	39.777348659220245	18.871528022932345
46	16.658429473149102	24.711227530112826	40.60553963031476	18.024803366423317
47	15.854420548738297	24.203404549755003	42.0518731378059	17.890301763700805
48	16.096064504285696	23.71093805159778	41.87077255461259	18.322224889503932
49	16.228271460242595	22.971179236623975	42.47285256364997	18.327696739483457
50	15.557352048237005	22.596269257381703	42.458908171766666	19.387470522614628
51	15.1644379174492	22.506071988364376	41.71314797294435	20.616342121242074
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	144.0
1	431.5
2	719.0
3	37635.5
4	74552.0
5	50087.5
6	25623.0
7	24706.0
8	23789.0
9	23206.0
10	22623.0
11	21681.0
12	20739.0
13	19315.5
14	17892.0
15	16567.5
16	15243.0
17	13900.5
18	12558.0
19	11606.0
20	10654.0
21	9512.5
22	8371.0
23	7590.0
24	6809.0
25	6605.5
26	6151.5
27	5901.0
28	6266.5
29	6632.0
30	6803.0
31	6974.0
32	7594.5
33	8215.0
34	9546.5
35	10878.0
36	11531.0
37	12184.0
38	12889.5
39	13595.0
40	15094.0
41	16593.0
42	17996.5
43	19400.0
44	20935.0
45	22470.0
46	25237.5
47	28005.0
48	28827.0
49	29649.0
50	29732.0
51	29815.0
52	26792.0
53	23769.0
54	20760.5
55	17752.0
56	16310.0
57	14868.0
58	14139.5
59	13411.0
60	12902.0
61	12393.0
62	11221.5
63	10050.0
64	8458.5
65	6867.0
66	5345.5
67	3824.0
68	3203.0
69	2582.0
70	2224.5
71	1867.0
72	1488.0
73	1109.0
74	827.0
75	525.0
76	505.0
77	428.5
78	352.0
79	241.5
80	131.0
81	80.0
82	29.0
83	28.5
84	28.0
85	24.0
86	20.0
87	11.0
88	2.0
89	1.5
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	566536.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.30777363707529
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.92059998238517	35.47798800250531
2	7.147584122738429	6.1909191047901855
3	2.420425824890169	3.1446976118902406
4	1.3010218209760716	2.253774340789088
5	0.8946212375076893	1.9372027022451592
6	0.6943879368026933	1.8043437350020068
7	0.570643254610759	1.729930222874467
8	0.46724098043625717	1.6188133291758844
9	0.4394943144028513	1.7130168254646256
>10	4.025719934146773	30.24220223627736
>50	0.05973988623022444	1.8325130411304416
>100	0.05405037325065655	3.833458177672345
>500	0.00406393783839523	1.2779367259574352
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	4.063937838395229E-4	6.943203944225426
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	39450	6.9633703771693245	No Hit
CCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	979	0.17280455257918298	RNA PCR Primer, Index 41 (96% over 26bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	925	0.16327294293743028	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	832	0.1468573929988562	No Hit
GCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	814	0.14368018978493863	Illumina PCR Primer Index 11 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	805	0.14209158817797987	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA	654	0.11543838343900477	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAA	595	0.10502421734894164	No Hit
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCT	590	0.1041416609006312	RNA PCR Primer, Index 41 (96% over 27bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.530225793241736E-4	0.0	0.0	0.11155513506643885	0.0
2	3.530225793241736E-4	0.0	0.0	0.5540689382492904	0.0
3	5.295338689862603E-4	0.0	0.0	0.7411709052911024	0.0
4	5.295338689862603E-4	0.0	0.0	0.9464535351681094	0.0
5	5.295338689862603E-4	0.0	0.0	1.51746755722496	0.0
6	5.295338689862603E-4	0.0	0.0	1.8191253512574663	0.0
7	5.295338689862603E-4	0.0	0.0	2.137198695228547	0.0
8	5.295338689862603E-4	0.0	0.0	2.6051301241227387	0.0
9	7.060451586483472E-4	0.0	0.0	2.765402375135914	0.0
10	7.060451586483472E-4	0.0	0.0	3.159905107530678	0.0
11	7.060451586483472E-4	0.0	0.0	3.7723992826581187	0.0
12	7.060451586483472E-4	0.0	0.0	4.31040569354816	0.0
13	7.060451586483472E-4	0.0	0.0	4.509157405707669	0.0
14	7.060451586483472E-4	0.0	0.0	4.585410282841691	0.0
15	8.82556448310434E-4	0.0	0.0	4.73085558552325	0.0
16	8.82556448310434E-4	0.0	0.0	5.022099213465693	0.0
17	8.82556448310434E-4	0.0	0.0	5.4166019458604575	0.0
18	8.82556448310434E-4	0.0	0.0	5.817635595972718	0.0
19	0.0010590677379725207	0.0	0.0	6.100936215880368	0.0
20	0.0010590677379725207	0.0	0.0	6.375058248725589	0.0
21	0.0012355790276346076	0.0	0.0	6.715372015194092	0.0
22	0.0014120903172966943	0.0	0.0	7.092400129912309	0.0
23	0.0014120903172966943	0.0	0.0	7.447187822133103	0.0
24	0.0014120903172966943	0.0	0.0	7.729958908171767	0.0
25	0.0014120903172966943	0.0	0.0	7.996314444271856	0.0
26	0.0014120903172966943	0.0	0.0	8.239723512715873	0.0
27	0.0014120903172966943	0.0	0.0	8.481014445683947	0.0
28	0.0014120903172966943	0.0	0.0	8.72407049154864	0.0
29	0.0014120903172966943	0.0	0.0	8.981600463165623	0.0
30	0.0014120903172966943	0.0	0.0	9.284670347515426	0.0
31	0.0014120903172966943	0.0	0.0	9.560204470677945	0.0
32	0.0014120903172966943	0.0	0.0	9.822500247115805	0.0
33	0.0014120903172966943	0.0	0.0	10.078794639705155	0.0
34	0.0014120903172966943	0.0	0.0	10.335089032294505	0.0
35	0.0014120903172966943	0.0	0.0	10.602150613553244	0.0
36	0.0014120903172966943	0.0	0.0	10.88280356411596	0.0
37	0.0014120903172966943	0.0	0.0	11.185167403307116	0.0
38	0.0014120903172966943	0.0	0.0	11.45875990228335	0.0
39	0.0014120903172966943	0.0	0.0	11.745590747984242	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGTAG	25	3.888292E-5	45.0	1
GCGTTAG	20	7.0302555E-4	45.0	1
TACGCGG	20	7.0302555E-4	45.0	2
TACGAGA	25	3.888292E-5	45.0	29
TCGTAAG	20	7.0302555E-4	45.0	1
CGTAACG	20	7.0302555E-4	45.0	1
CGTAAAT	20	7.0302555E-4	45.0	14
AAGGGCG	85	0.0	45.0	5
TGTACGG	20	7.0302555E-4	45.0	2
TGCGTTG	20	7.0302555E-4	45.0	1
CGTTTTT	24600	0.0	44.780487	1
CGGGTAT	60	3.6379788E-12	41.249996	6
TGCGCGG	60	3.6379788E-12	41.249996	2
GTTTTTT	26900	0.0	41.06877	2
GCTACGA	55	6.002665E-11	40.909092	10
ATAGCGG	50	1.0786607E-9	40.5	2
TGCGAAG	50	1.0786607E-9	40.5	1
CACATCC	80	0.0	39.375	45
GTAGCGG	40	3.454079E-7	39.375	2
TCGTAGG	40	3.454079E-7	39.375	2
>>END_MODULE