SRR3550275_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550275_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1185566
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

$Duplication level graph$

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGC	3392	0.28610806990079	RNA PCR Primer, Index 30 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3289	0.2774202364102884	No Hit
GCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGC	3241	0.27337153730791874	RNA PCR Primer, Index 23 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGCT	2430	0.20496539205746453	RNA PCR Primer, Index 30 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGC	1471	0.12407575790803717	RNA PCR Primer, Index 23 (96% over 25bp)
ACTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGC	1198	0.10104878176330967	RNA PCR Primer, Index 23 (96% over 25bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAC	20	7.033376E-4	45.000004	10
CGTATCA	30	2.1655596E-6	45.000004	22
TACGCGA	20	7.033376E-4	45.000004	39
GAACGTA	20	7.033376E-4	45.000004	9
CGTAATG	20	7.033376E-4	45.000004	25
TTCGCGC	30	2.1655596E-6	45.000004	22
TATCGTG	30	2.1655596E-6	45.000004	1
TATTACG	25	3.8908813E-5	45.0	1
GCGATTC	60	3.6379788E-12	41.250004	9
CGTTTTT	2260	0.0	39.424778	1
GCGTAAG	80	0.0	39.375004	1
TATTGCG	110	0.0	38.863632	1
CGTAAGG	145	0.0	38.793102	2
TCGATAG	35	6.248454E-6	38.57143	1
TCGTAAG	35	6.248454E-6	38.57143	1
CACGTAT	35	6.248454E-6	38.57143	20
TAATACG	65	9.094947E-12	38.076927	1
TTACACG	320	0.0	37.968754	34
ACACGCG	335	0.0	37.61194	36
ATTCGAT	30	1.13994334E-4	37.500004	17