SRR3550273_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550273_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1206443
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4927	0.40839061605065474	No Hit
GCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGC	3690	0.3058577985035348	TruSeq Adapter, Index 21 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGC	3122	0.25877724848998257	TruSeq Adapter, Index 21 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGCT	2482	0.2057287414324589	Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTG	1420	0.11770137503388058	No Hit
TCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGC	1392	0.11538050285011393	TruSeq Adapter, Index 21 (95% over 23bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTAAG	25	3.8909202E-5	45.000004	1
TTACGCG	25	3.8909202E-5	45.000004	1
ACGTACG	25	3.8909202E-5	45.000004	1
TCCGATC	20	7.033426E-4	45.0	15
TAAGGCG	70	0.0	45.0	1
ACGTATG	20	7.033426E-4	45.0	1
TCGACGT	45	3.8562575E-10	45.0	26
CGTTTTT	3455	0.0	41.09262	1
TTGCGAG	155	0.0	40.645164	1
TAGCGCG	50	1.0822987E-9	40.500004	1
TAGTCCG	50	1.0822987E-9	40.500004	1
TAAACGG	90	0.0	40.0	2
CTAACGG	45	1.9284926E-8	40.0	2
CGGTCTA	45	1.9284926E-8	40.0	31
TTAACGG	85	0.0	39.705883	2
ACGATAG	40	3.4584082E-7	39.375	1
GTTAACG	40	3.4584082E-7	39.375	1
CTCGGTT	35	6.248547E-6	38.571426	13
TCCGTCA	35	6.248547E-6	38.571426	29
CACGTTA	35	6.248547E-6	38.571426	16