FastQCFastQC Report
Sat 18 Jun 2016
SRR3550272_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550272_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences969506
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGC36430.3757583759151568No Hit
CCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGC32450.33470654126947125No Hit
CTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGCT22940.23661534843518245Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21570.22248444052950675No Hit
TCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGC11330.11686363983306963No Hit
ACTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGC10760.1109843569818031No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGT351.2116652E-745.00000425
AGATCGC207.0327386E-445.00000429
CGCGGAT253.890352E-545.00000423
TTAGCCG351.2116652E-745.0000041
GACGTGC207.0327386E-445.00000417
CGCGACT253.890352E-545.00000431
CGACACG207.0327386E-445.00000427
ATCGTCC406.8139343E-945.00000426
AACGGCG253.890352E-545.00000413
TCGGACT253.890352E-545.00000415
CTAAACG207.0327386E-445.0000041
TGCGACC207.0327386E-445.00000417
ACGGACT207.0327386E-445.0000045
TTGCGAA253.890352E-545.00000444
ACGTCAC207.0327386E-445.00000414
TATAGCG207.0327386E-445.0000041
ATTGCCG351.2116652E-745.0000041
TTCGCGA253.890352E-545.00000429
CGTTTAT207.0327386E-445.00000412
TCGAACG207.0327386E-445.0000041