##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550272_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	969506
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.41399124915163	33.0	31.0	34.0	31.0	34.0
2	32.57918775128777	34.0	31.0	34.0	31.0	34.0
3	32.61008389839774	34.0	31.0	34.0	31.0	34.0
4	36.088682277366	37.0	35.0	37.0	35.0	37.0
5	36.06842247495116	37.0	35.0	37.0	35.0	37.0
6	36.102440830691094	37.0	35.0	37.0	35.0	37.0
7	36.262024164883975	37.0	37.0	37.0	35.0	37.0
8	36.226100715209604	37.0	37.0	37.0	35.0	37.0
9	38.0790103413491	39.0	39.0	39.0	37.0	39.0
10	37.73074947447463	39.0	37.0	39.0	35.0	39.0
11	37.5883676841608	39.0	37.0	39.0	35.0	39.0
12	37.42055025961675	39.0	37.0	39.0	35.0	39.0
13	37.357541882154415	39.0	37.0	39.0	35.0	39.0
14	38.58825112995691	40.0	38.0	41.0	35.0	41.0
15	38.64503778212822	40.0	38.0	41.0	35.0	41.0
16	38.69254857628524	40.0	38.0	41.0	35.0	41.0
17	38.72734155332716	40.0	38.0	41.0	35.0	41.0
18	38.67933566166687	40.0	38.0	41.0	35.0	41.0
19	38.64506666281591	40.0	38.0	41.0	35.0	41.0
20	38.565702533042604	40.0	38.0	41.0	35.0	41.0
21	38.54079087700334	40.0	38.0	41.0	35.0	41.0
22	38.50953681565663	40.0	38.0	41.0	35.0	41.0
23	38.481676235113554	40.0	37.0	41.0	35.0	41.0
24	38.48371748086139	40.0	37.0	41.0	35.0	41.0
25	38.412045928545055	40.0	37.0	41.0	34.0	41.0
26	38.32342141255444	40.0	37.0	41.0	34.0	41.0
27	38.28116896646333	40.0	37.0	41.0	34.0	41.0
28	38.225169313031586	40.0	37.0	41.0	34.0	41.0
29	38.19568006799339	40.0	37.0	41.0	34.0	41.0
30	38.14648491087213	40.0	37.0	41.0	34.0	41.0
31	38.05285887864542	40.0	37.0	41.0	34.0	41.0
32	37.910220256501766	40.0	37.0	41.0	34.0	41.0
33	37.82155448238588	40.0	37.0	41.0	34.0	41.0
34	37.66877564450349	40.0	36.0	41.0	33.0	41.0
35	37.59381066233731	40.0	36.0	41.0	33.0	41.0
36	37.520016379475734	40.0	36.0	41.0	33.0	41.0
37	37.40867617116346	40.0	36.0	41.0	33.0	41.0
38	37.35443308241517	40.0	36.0	41.0	33.0	41.0
39	37.31181137610288	40.0	36.0	41.0	33.0	41.0
40	37.22685058163642	40.0	36.0	41.0	32.0	41.0
41	37.18394109990036	40.0	36.0	41.0	32.0	41.0
42	37.09530936373782	40.0	35.0	41.0	32.0	41.0
43	36.99576175908143	39.0	35.0	41.0	32.0	41.0
44	36.88377689256178	39.0	35.0	41.0	31.0	41.0
45	36.82085309425625	39.0	35.0	41.0	31.0	41.0
46	36.77661303798017	39.0	35.0	41.0	31.0	41.0
47	36.69629171970055	39.0	35.0	41.0	31.0	41.0
48	36.67892411186728	39.0	35.0	41.0	31.0	41.0
49	36.57383863534625	39.0	35.0	41.0	31.0	41.0
50	36.456051844960214	39.0	35.0	41.0	31.0	41.0
51	35.47317602985438	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	14.0
10	13.0
11	26.0
12	17.0
13	15.0
14	18.0
15	30.0
16	47.0
17	69.0
18	121.0
19	235.0
20	418.0
21	763.0
22	1194.0
23	1882.0
24	3078.0
25	4796.0
26	6564.0
27	7673.0
28	8232.0
29	9573.0
30	11299.0
31	14014.0
32	18621.0
33	25493.0
34	49198.0
35	76425.0
36	59820.0
37	95980.0
38	196164.0
39	377322.0
40	388.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.97540396861907	22.74519188122611	25.503813282228272	10.775590867926551
2	31.154010392921755	24.951366984835577	26.199837855567683	17.694784766674985
3	31.00403710755787	24.657196551645892	27.640262154127974	16.698504186668263
4	27.500190818829385	27.153313130604655	26.672140244619424	18.674355805946533
5	24.95239843796738	30.837044845519262	25.45151860844595	18.759038108067408
6	26.71752418241867	33.604536743454915	24.853688373254005	14.824250700872401
7	82.13461288532511	5.050716550490662	8.418307880508218	4.396362683676017
8	83.90169839072682	4.114157106815224	7.416457453589766	4.567687048868187
9	78.0458295255522	7.079791151369872	9.640579841692572	5.233799481385365
10	38.570364701198336	32.30335861768777	16.389377683067462	12.736898998046428
11	28.308128056969217	26.9917875701646	26.43490602430516	18.26517834856102
12	26.261931334102112	25.327641087316632	29.06882474167256	19.341602836908695
13	23.752612155056283	26.957543326188798	30.94132475714436	18.34851976161055
14	20.461864083357916	30.389909912883468	29.061707715063136	20.08651828869548
15	19.145420451240117	28.635511281002902	33.739657103720866	18.479411164036115
16	21.44195084919536	28.800131200838365	30.214253444537732	19.543664505428538
17	22.460201380909453	26.491945382493764	28.02994514732245	23.01790808927433
18	22.247103163879338	26.952076624590255	30.895115656839668	19.90570455469074
19	22.145092449144204	28.945256656482787	28.132574733936664	20.777076160436344
20	24.374887829471916	28.29069649904178	28.398999077880898	18.935416593605403
21	23.26019642993442	28.30204248349159	29.26789519611018	19.169865890463804
22	22.15179689450091	26.160848927185597	28.268313966081692	23.419040212231796
23	20.07816351832789	28.791260703904875	28.92978485950577	22.200790918261465
24	21.72745707607792	26.60365175666783	29.846540403050625	21.822350764203623
25	19.561508644608697	29.537517044763	28.43159299684582	22.46938131378248
26	19.589976751046407	31.178662122771804	27.983014029825497	21.248347096356287
27	21.182746677173736	30.215181752356358	28.358153533861575	20.243918036608335
28	18.459813554531895	29.37042163741122	30.229003224322494	21.940761583734396
29	19.695597551742846	28.40797272012757	29.62457168908702	22.271858039042563
30	21.922917444554237	28.81405581811768	28.76268945215398	20.5003372851741
31	22.020802346762167	30.36577390959932	25.638521061241498	21.974902682397015
32	22.587070116120994	31.76122685161309	24.984889211619112	20.666813820646805
33	22.825851516132957	28.981048080156285	26.962597446534627	21.230502957176128
34	20.14149474062048	29.26789519611018	27.640055863501615	22.950554199767716
35	21.42761365066333	29.318333254255258	26.73062363719255	22.523429457888863
36	23.879377744954645	29.075013460463374	26.491326510614684	20.5542822839673
37	22.08124550028571	29.76856254628646	27.881312751029906	20.268879202397923
38	21.66288811002717	30.675725575705563	26.095661089255767	21.5657252250115
39	21.626374669161407	28.354234011960735	27.877290083815883	22.142101235061983
40	22.80202494878835	26.317526657906193	28.47821467840323	22.402233714902227
41	22.040090520326846	26.918863833746258	26.618710972392122	24.422334673534767
42	24.01790190055554	27.165587422873095	26.214071908786536	22.60243876778483
43	23.72001823609137	27.490701450016815	26.478742782406712	22.310537531485107
44	20.895074398714396	28.95639635030624	26.43686578525558	23.71166346572378
45	20.876198806402435	28.24747861281931	26.420878261712666	24.455444319065585
46	22.689596557422025	28.002199058077	27.1038033802782	22.204401004222767
47	21.145098637862993	27.204988932507895	28.36351709014694	23.28639533948217
48	22.400377099264986	26.00819386367903	28.24386852685801	23.347560510197976
49	22.615950803811423	24.975606133432905	29.61126594368679	22.797177119068888
50	21.200487671040715	25.317945427877703	28.98146066140901	24.500106239672576
51	20.903429169081985	25.29989499807118	26.817678281516567	26.978997551330263
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	239.0
1	509.0
2	779.0
3	2454.0
4	4129.0
5	2987.0
6	1845.0
7	1922.0
8	1999.0
9	2004.5
10	2010.0
11	2083.0
12	2156.0
13	2147.0
14	2138.0
15	2142.0
16	2146.0
17	2135.5
18	2125.0
19	2341.5
20	2558.0
21	2736.0
22	2914.0
23	3246.0
24	3578.0
25	4551.5
26	6392.5
27	7260.0
28	8769.5
29	10279.0
30	12369.0
31	14459.0
32	17179.5
33	19900.0
34	23223.5
35	26547.0
36	30452.5
37	34358.0
38	38401.5
39	42445.0
40	47901.5
41	53358.0
42	59645.5
43	65933.0
44	74231.5
45	82530.0
46	93526.5
47	104523.0
48	111325.0
49	118127.0
50	112961.5
51	107796.0
52	90341.0
53	72886.0
54	60832.0
55	48778.0
56	41795.5
57	34813.0
58	30539.0
59	26265.0
60	23653.0
61	21041.0
62	18392.0
63	15743.0
64	13103.5
65	10464.0
66	8550.5
67	6637.0
68	5648.5
69	4660.0
70	3814.5
71	2969.0
72	2135.0
73	1301.0
74	1079.0
75	789.5
76	722.0
77	573.5
78	425.0
79	264.5
80	104.0
81	108.0
82	112.0
83	67.0
84	22.0
85	33.5
86	45.0
87	24.5
88	4.0
89	2.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	969506.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.3870843696981
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.16773964905661	20.326382007281392
2	12.971811709093009	7.624074502459079
3	5.147423977398684	4.538033481312662
4	2.5742997392054616	3.026046545156909
5	1.5246573201853364	2.2402616651582146
6	0.9867774699136612	1.7399107657481996
7	0.6883274207864208	1.4159555192039064
8	0.5333852997598058	1.2539711044478499
9	0.44132769112097014	1.167240068429041
>10	5.040136958466779	35.51987541095182
>50	0.8574205729329147	16.093507355270372
>100	0.06243524364971198	3.2134883378318086
>500	0.0021284742153307888	0.428882109386503
>1k	0.0021284742153307888	1.4123711273622614
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGC	3643	0.3757583759151568	No Hit
CCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGC	3245	0.33470654126947125	No Hit
CTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGCT	2294	0.23661534843518245	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2157	0.22248444052950675	No Hit
TCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGC	1133	0.11686363983306963	No Hit
ACTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGC	1076	0.1109843569818031	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.24961165789587686	0.0
2	0.0	0.0	0.0	1.2320707659364667	0.0
3	0.0	0.0	0.0	1.7489319302820199	0.0
4	0.0	0.0	0.0	2.345730712342162	0.0
5	0.0	0.0	0.0	3.8723844927210354	0.0
6	0.0	0.0	0.0	4.6811468933663125	0.0
7	0.0	0.0	0.0	5.565927389825334	0.0
8	0.0	0.0	0.0	6.964371545921325	0.0
9	0.0	0.0	0.0	7.460087921064955	0.0
10	0.0	0.0	0.0	8.698141115165868	0.0
11	0.0	0.0	0.0	10.507000472405535	0.0
12	0.0	0.0	0.0	11.960421080426526	0.0
13	0.0	0.0	0.0	12.515858591901443	0.0
14	0.0	0.0	0.0	12.734320365216925	0.0
15	0.0	0.0	0.0	13.141950642904737	0.0
16	0.0	0.0	0.0	14.034363892539087	0.0
17	0.0	0.0	0.0	15.21805950659408	0.0
18	0.0	0.0	0.0	16.51810303391624	0.0
19	0.0	0.0	0.0	17.323255348600213	0.0
20	0.0	0.0	0.0	18.072606048853746	0.0
21	0.0	0.0	0.0	19.0402122317964	0.0
22	0.0	0.0	0.0	20.06805527763624	0.0
23	0.0	0.0	0.0	21.140250808143527	0.0
24	0.0	0.0	0.0	21.92580551332328	0.0
25	0.0	0.0	0.0	22.643387457117335	0.0
26	0.0	0.0	0.0	23.340340338275368	0.0
27	0.0	0.0	0.0	23.986339434722424	0.0
28	0.0	0.0	0.0	24.681641990869576	0.0
29	0.0	0.0	0.0	25.413561133195667	0.0
30	0.0	0.0	0.0	26.254814307492683	0.0
31	1.0314531318011441E-4	0.0	0.0	26.988074338890115	0.0
32	1.0314531318011441E-4	0.0	0.0	27.680695116894583	0.0
33	1.0314531318011441E-4	0.0	0.0	28.367333466734603	0.0
34	1.0314531318011441E-4	0.0	0.0	29.03984090866895	0.0
35	1.0314531318011441E-4	0.0	0.0	29.776195299461786	0.0
36	1.0314531318011441E-4	0.0	0.0	30.470260111850777	0.0
37	1.0314531318011441E-4	0.0	0.0	31.175155182123678	0.0
38	1.0314531318011441E-4	0.0	0.0	31.848075205310746	0.0
39	1.0314531318011441E-4	0.0	0.0	32.549463334935524	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCGT	35	1.2116652E-7	45.000004	25
AGATCGC	20	7.0327386E-4	45.000004	29
CGCGGAT	25	3.890352E-5	45.000004	23
TTAGCCG	35	1.2116652E-7	45.000004	1
GACGTGC	20	7.0327386E-4	45.000004	17
CGCGACT	25	3.890352E-5	45.000004	31
CGACACG	20	7.0327386E-4	45.000004	27
ATCGTCC	40	6.8139343E-9	45.000004	26
AACGGCG	25	3.890352E-5	45.000004	13
TCGGACT	25	3.890352E-5	45.000004	15
CTAAACG	20	7.0327386E-4	45.000004	1
TGCGACC	20	7.0327386E-4	45.000004	17
ACGGACT	20	7.0327386E-4	45.000004	5
TTGCGAA	25	3.890352E-5	45.000004	44
ACGTCAC	20	7.0327386E-4	45.000004	14
TATAGCG	20	7.0327386E-4	45.000004	1
ATTGCCG	35	1.2116652E-7	45.000004	1
TTCGCGA	25	3.890352E-5	45.000004	29
CGTTTAT	20	7.0327386E-4	45.000004	12
TCGAACG	20	7.0327386E-4	45.000004	1
>>END_MODULE