FastQCFastQC Report
Sat 18 Jun 2016
SRR3550271_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550271_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences665642
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC25680.38579296378533806No Hit
CCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC17570.26395569991076284No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15270.22940259178357136No Hit
CTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGCT12070.18132870221530492No Hit
TCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC10110.15188344485474176No Hit
ACTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC7710.11582802767854193No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGGC207.031145E-445.044
CTCGTAG207.031145E-445.01
ACGTCGT207.031145E-445.025
TGTAGCG207.031145E-445.01
TAGTACG207.031145E-445.01
GTTAACG253.8890314E-544.9999961
TGATTCG302.1641208E-644.99999615
TTGCGAG253.8890314E-544.9999961
AATAGCG253.8890314E-544.9999961
CACGTAG253.8890314E-544.9999961
TCGACGG302.1641208E-644.9999962
TTCACGG650.041.538462
GTAAGCG603.6379788E-1241.2499961
CGTTTTT9950.041.155781
ATATGCG556.002665E-1140.909091
ATGCGTC501.0804797E-940.49999624
TTGCACG403.4552977E-739.3751
ATAGGGC2650.039.0566024
ATTCGCG356.244327E-638.5714261
CGGGCTA700.038.5714266
Produced by FastQC (version 0.11.5)