##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550271_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	665642
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.413518077284785	33.0	31.0	34.0	31.0	34.0
2	32.591331376325414	34.0	31.0	34.0	31.0	34.0
3	32.632732009097985	34.0	31.0	34.0	31.0	34.0
4	36.08402264280198	37.0	35.0	37.0	35.0	37.0
5	36.05416725507104	37.0	35.0	37.0	35.0	37.0
6	36.07955627799929	37.0	35.0	37.0	35.0	37.0
7	36.20283425625186	37.0	36.0	37.0	35.0	37.0
8	36.142152989144314	37.0	36.0	37.0	35.0	37.0
9	37.96952866555896	39.0	38.0	39.0	35.0	39.0
10	37.64785124736721	39.0	37.0	39.0	35.0	39.0
11	37.483663891401086	39.0	37.0	39.0	35.0	39.0
12	37.31569372124956	39.0	37.0	39.0	35.0	39.0
13	37.22113688739593	39.0	37.0	39.0	34.0	39.0
14	38.45160461629525	40.0	38.0	41.0	34.0	41.0
15	38.52760192415743	40.0	38.0	41.0	34.0	41.0
16	38.585331153983674	40.0	38.0	41.0	35.0	41.0
17	38.59837269883811	40.0	38.0	41.0	35.0	41.0
18	38.562133999957936	40.0	38.0	41.0	35.0	41.0
19	38.53480098912028	40.0	37.0	41.0	35.0	41.0
20	38.438863533250604	40.0	37.0	41.0	34.0	41.0
21	38.42585503919524	40.0	37.0	41.0	34.0	41.0
22	38.3844859549127	40.0	37.0	41.0	34.0	41.0
23	38.350152183906665	40.0	37.0	41.0	34.0	41.0
24	38.30981668824984	40.0	37.0	41.0	34.0	41.0
25	38.24424690749682	40.0	37.0	41.0	34.0	41.0
26	38.16422190907425	40.0	37.0	41.0	34.0	41.0
27	38.10991794387974	40.0	37.0	41.0	34.0	41.0
28	38.08086929610812	40.0	37.0	41.0	34.0	41.0
29	38.0570381676637	40.0	36.0	41.0	34.0	41.0
30	37.981049873655806	40.0	36.0	41.0	34.0	41.0
31	37.893490795352456	40.0	36.0	41.0	34.0	41.0
32	37.77957971402045	40.0	36.0	41.0	33.0	41.0
33	37.71168586116862	40.0	36.0	41.0	33.0	41.0
34	37.60431433112694	40.0	36.0	41.0	33.0	41.0
35	37.49768644406453	40.0	36.0	41.0	33.0	41.0
36	37.41474546377783	40.0	36.0	41.0	33.0	41.0
37	37.364167225024865	40.0	35.0	41.0	33.0	41.0
38	37.28335050973346	40.0	35.0	41.0	33.0	41.0
39	37.2390564297325	40.0	35.0	41.0	33.0	41.0
40	37.130906703603436	39.0	35.0	41.0	32.0	41.0
41	37.05069842347688	39.0	35.0	41.0	32.0	41.0
42	36.98380811306979	39.0	35.0	41.0	32.0	41.0
43	36.94003984123598	39.0	35.0	41.0	32.0	41.0
44	36.89282977937089	39.0	35.0	41.0	32.0	41.0
45	36.798684578196685	39.0	35.0	41.0	31.0	41.0
46	36.70992215034508	39.0	35.0	41.0	31.0	41.0
47	36.62386538109074	39.0	35.0	41.0	31.0	41.0
48	36.602033825990546	39.0	35.0	41.0	31.0	41.0
49	36.52971717529844	39.0	35.0	41.0	31.0	41.0
50	36.44742669482995	39.0	35.0	41.0	31.0	41.0
51	35.54838787215951	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	10.0
10	15.0
11	18.0
12	15.0
13	16.0
14	17.0
15	24.0
16	45.0
17	72.0
18	151.0
19	256.0
20	415.0
21	596.0
22	909.0
23	1421.0
24	2095.0
25	3090.0
26	4314.0
27	5143.0
28	5959.0
29	6759.0
30	8175.0
31	10111.0
32	13063.0
33	18755.0
34	37228.0
35	54377.0
36	43099.0
37	67377.0
38	131004.0
39	250845.0
40	264.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.452462434762225	22.849820173606833	24.364147695007226	11.33356969662371
2	31.222338734635137	25.230529323570327	25.123114226566233	18.424017715228306
3	31.10440747428798	24.898669254644386	26.87721027218835	17.119712998879276
4	28.16138404728067	26.254202709564602	27.429008385889116	18.155404857265616
5	25.681672730987525	30.45646158145069	25.006835506172987	18.855030181388795
6	26.75387069926477	33.950081274919555	24.275962153830438	15.020085871985241
7	81.198301789851	6.146847704922466	8.142515045625126	4.512335459601408
8	82.39804579638906	4.860270235351736	7.713605812133249	5.028078156125965
9	76.17142548096425	7.685813094726594	10.03767791094913	6.105083513360034
10	41.98022360367886	26.029156814023153	17.4308412029289	14.55977837936909
11	30.044378209307705	27.184432472710558	24.11791924187476	18.653270076106978
12	27.98321019406828	24.069244428686893	28.739472569339075	19.20807280790575
13	24.339960519318193	26.140327683649772	30.338981013818238	19.1807307832138
14	20.295894790292678	29.147649937954633	28.490990652633098	22.065464619119588
15	19.62856911072318	27.291246646095047	32.29769155191529	20.782492691266476
16	21.92184387403439	27.33301083765748	29.780572740301842	20.964572548006284
17	22.184147033991245	26.757776702792192	27.254139612584545	23.80393665063202
18	22.150495311293458	26.967649276938655	29.99675501245414	20.885100399313743
19	23.43572070272008	27.56571850934887	27.532968172080487	21.46559261585056
20	24.164791284203822	28.00258998080049	27.63016756755133	20.202451167444362
21	23.783204785755707	27.804735879046095	28.17760898500996	20.23445035018824
22	22.83795193211967	26.01353279991347	26.963743273411232	24.184771994555632
23	21.544313610018598	27.47377719554956	27.681246075217608	23.30066311921423
24	22.2484458612888	26.56638252994853	29.10378251372359	22.081389095039075
25	20.922658125538955	28.36870269604382	27.505626147388533	23.20301303102869
26	21.013848284813758	28.68223459457186	27.31543382178408	22.988483298830303
27	20.935577983360425	28.443968379399138	27.407976059202994	23.212477578037443
28	19.184937248551023	27.444181707284095	28.85605175154212	24.51482929262276
29	19.842347688397066	25.85518942614799	28.853047133444104	25.44941575201084
30	21.578265794526185	26.53092803639193	26.7594292427461	25.13137692633578
31	21.871366289987712	28.093179216455695	24.99271380111231	25.042740692444287
32	21.470400004807388	28.245513354025135	25.48682324733115	24.797263393836328
33	21.079198728445622	28.41016642579645	24.875533695289658	25.63510115046827
34	19.818761436327637	27.3007111931038	26.876909810378553	26.003617560190012
35	19.495915221695746	27.635425649222856	26.797287430781108	26.07137169830029
36	20.60942668882072	29.074787949077734	25.069632024421534	25.246153337680017
37	20.16083720678683	29.33093164193365	26.84370878039547	23.664522370884047
38	19.334416998927352	29.704856364231823	26.503886473509787	24.45684016333104
39	21.218162315478892	28.355031683697845	27.09910131872688	23.327704682096382
40	21.880680606091563	27.64188557813359	27.288842951616637	23.18859086415821
41	19.266813091721975	29.185508125989646	25.044994156017797	26.50268462627058
42	20.225136034084386	27.75350714047491	26.17983841163869	25.84151841380201
43	21.316413327284035	25.953891130667838	27.150179826393167	25.57951571565496
44	20.35929223216083	25.909122321007388	26.62962974091178	27.10195570592
45	20.58283581865327	26.038621361031904	26.51229940418423	26.86624341613059
46	21.455827607032006	27.307321352919438	26.368528428194132	24.86832261185442
47	19.22024151120272	27.402717977531466	28.124427244675065	25.25261326659075
48	19.96583749222555	26.069568927441477	28.260386213610317	25.704207366722652
49	20.57772796788664	24.67136989552943	29.479209545070773	25.271692591513155
50	19.784358559105346	24.94373852611464	28.976236475462784	26.295666439317227
51	19.582598453823525	25.591984880761732	26.212588748907066	28.612827916507673
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	146.0
1	442.5
2	739.0
3	1811.5
4	2884.0
5	2067.0
6	1250.0
7	1226.0
8	1202.0
9	1220.5
10	1239.0
11	1254.0
12	1269.0
13	1307.0
14	1345.0
15	1265.0
16	1185.0
17	1201.0
18	1217.0
19	1116.0
20	1015.0
21	1305.5
22	1596.0
23	1885.5
24	2175.0
25	2873.0
26	4068.0
27	4565.0
28	6681.5
29	8798.0
30	9872.5
31	10947.0
32	12202.0
33	13457.0
34	15353.5
35	17250.0
36	19841.5
37	22433.0
38	23849.5
39	25266.0
40	29223.0
41	33180.0
42	36303.5
43	39427.0
44	44389.5
45	49352.0
46	55742.0
47	62132.0
48	67427.0
49	72722.0
50	69822.0
51	66922.0
52	57083.5
53	47245.0
54	41931.0
55	36617.0
56	32670.0
57	28723.0
58	27055.0
59	25387.0
60	23325.5
61	21264.0
62	19413.0
63	17562.0
64	15184.0
65	12806.0
66	10887.0
67	8968.0
68	7775.5
69	6583.0
70	5661.5
71	4740.0
72	4216.5
73	3693.0
74	2910.0
75	1712.5
76	1298.0
77	979.0
78	660.0
79	501.5
80	343.0
81	290.0
82	237.0
83	156.5
84	76.0
85	44.0
86	12.0
87	10.0
88	8.0
89	5.5
90	3.0
91	4.0
92	5.0
93	3.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	665642.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.57326000944474
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.78218541014189	21.228332331802967
2	11.018854628578795	6.517269058744687
3	4.03362854317137	3.5786263706617416
4	2.030436758184867	2.4018653673014057
5	1.2404763205407185	1.8342464381454993
6	0.8702277631119304	1.5441283143567959
7	0.67550468125774	1.3983812903502566
8	0.5772156991703613	1.3656119962478823
9	0.5010862568387079	1.3336878744585465
>10	6.857720020967806	48.5167791460365
>50	0.36345182346262667	6.4728743995261
>100	0.04511108669204138	2.3081132803860123
>500	0.0015378779554105016	0.2766703932154005
>1k	0.002563129925684169	1.2234137387661819
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC	2568	0.38579296378533806	No Hit
CCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC	1757	0.26395569991076284	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1527	0.22940259178357136	No Hit
CTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGCT	1207	0.18132870221530492	No Hit
TCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC	1011	0.15188344485474176	No Hit
ACTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC	771	0.11582802767854193	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.18944117107994987	0.0
2	0.0	0.0	0.0	1.1498673461109725	0.0
3	0.0	0.0	0.0	1.6481832576670343	0.0
4	0.0	0.0	0.0	2.190216362549238	0.0
5	0.0	0.0	0.0	3.702440651281019	0.0
6	0.0	0.0	0.0	4.559507963740269	0.0
7	0.0	0.0	0.0	5.450527460707107	0.0
8	0.0	0.0	0.0	6.819281235258592	0.0
9	0.0	0.0	0.0	7.246087236081858	0.0
10	0.0	0.0	0.0	8.310923890018959	0.0
11	0.0	0.0	0.0	9.885644235189487	0.0
12	0.0	0.0	0.0	11.030704192343633	0.0
13	0.0	0.0	0.0	11.484401525144147	0.0
14	0.0	0.0	0.0	11.655965218540898	0.0
15	0.0	0.0	0.0	12.022378395594028	0.0
16	0.0	0.0	0.0	12.814696188041019	0.0
17	0.0	0.0	0.0	13.758296501723148	0.0
18	0.0	0.0	0.0	14.758684097457792	0.0
19	0.0	0.0	0.0	15.350744093671974	0.0
20	0.0	0.0	0.0	15.92567776672746	0.0
21	0.0	0.0	0.0	16.664813818839555	0.0
22	0.0	0.0	0.0	17.460286460289463	0.0
23	0.0	0.0	0.0	18.256209794454076	0.0
24	0.0	0.0	0.0	18.907610998104087	0.0
25	0.0	0.0	0.0	19.47142758419691	0.0
26	0.0	0.0	0.0	19.988822820675377	0.0
27	0.0	0.0	0.0	20.498406050099003	0.0
28	0.0	0.0	0.0	21.020007751914694	0.0
29	0.0	0.0	0.0	21.577965332716385	0.0
30	0.0	0.0	0.0	22.184597726705945	0.0
31	0.0	0.0	0.0	22.730837296925376	0.0
32	0.0	0.0	0.0	23.25334038417047	0.0
33	0.0	0.0	0.0	23.772237929697944	0.0
34	0.0	0.0	0.0	24.29444055513324	0.0
35	0.0	0.0	0.0	24.864867301041702	0.0
36	0.0	0.0	0.0	25.378807226707448	0.0
37	0.0	0.0	0.0	25.894249461422206	0.0
38	0.0	0.0	0.0	26.42411386300744	0.0
39	0.0	0.0	0.0	26.933847323335968	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGGC	20	7.031145E-4	45.0	44
CTCGTAG	20	7.031145E-4	45.0	1
ACGTCGT	20	7.031145E-4	45.0	25
TGTAGCG	20	7.031145E-4	45.0	1
TAGTACG	20	7.031145E-4	45.0	1
GTTAACG	25	3.8890314E-5	44.999996	1
TGATTCG	30	2.1641208E-6	44.999996	15
TTGCGAG	25	3.8890314E-5	44.999996	1
AATAGCG	25	3.8890314E-5	44.999996	1
CACGTAG	25	3.8890314E-5	44.999996	1
TCGACGG	30	2.1641208E-6	44.999996	2
TTCACGG	65	0.0	41.53846	2
GTAAGCG	60	3.6379788E-12	41.249996	1
CGTTTTT	995	0.0	41.15578	1
ATATGCG	55	6.002665E-11	40.90909	1
ATGCGTC	50	1.0804797E-9	40.499996	24
TTGCACG	40	3.4552977E-7	39.375	1
ATAGGGC	265	0.0	39.056602	4
ATTCGCG	35	6.244327E-6	38.571426	1
CGGGCTA	70	0.0	38.571426	6
>>END_MODULE