Basic Statistics
Measure | Value |
---|---|
Filename | SRR3550269_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 898097 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 49 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGCT | 4840 | 0.538917288444344 | Illumina Single End Adapter 1 (95% over 21bp) |
GCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGC | 3892 | 0.4333607616994601 | TruSeq Adapter, Index 15 (95% over 22bp) |
CCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGC | 2755 | 0.30675973753391894 | TruSeq Adapter, Index 15 (95% over 22bp) |
TCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGC | 2042 | 0.22736964938085752 | TruSeq Adapter, Index 15 (95% over 22bp) |
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCC | 1799 | 0.20031243841144106 | No Hit |
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 1789 | 0.19919897293944863 | No Hit |
ACTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGC | 1220 | 0.13584278758307844 | No Hit |
GCCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTG | 1043 | 0.11613444872881215 | TruSeq Adapter, Index 15 (95% over 21bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTGCCG | 20 | 7.032462E-4 | 45.0 | 10 |
ATTACGC | 20 | 7.032462E-4 | 45.0 | 45 |
ATTCGCG | 20 | 7.032462E-4 | 45.0 | 1 |
ACGATAC | 20 | 7.032462E-4 | 45.0 | 22 |
GTATGCG | 25 | 3.8901213E-5 | 45.0 | 1 |
CGCTAGA | 20 | 7.032462E-4 | 45.0 | 33 |
ACGGAAC | 25 | 3.8901213E-5 | 45.0 | 1 |
CGAATTA | 20 | 7.032462E-4 | 45.0 | 25 |
CGAATAT | 70 | 0.0 | 45.0 | 14 |
TTAACGA | 20 | 7.032462E-4 | 45.0 | 22 |
AATTACG | 20 | 7.032462E-4 | 45.0 | 44 |
TTACGAG | 35 | 1.2115379E-7 | 45.0 | 1 |
TACAGCG | 25 | 3.8901213E-5 | 45.0 | 1 |
CGATACT | 20 | 7.032462E-4 | 45.0 | 10 |
CCCGCAA | 20 | 7.032462E-4 | 45.0 | 40 |
AAATGCG | 45 | 3.8380676E-10 | 45.0 | 1 |
ACGAATT | 20 | 7.032462E-4 | 45.0 | 24 |
CCCGATC | 25 | 3.8901213E-5 | 45.0 | 41 |
TCGTTCA | 30 | 2.1649703E-6 | 44.999996 | 16 |
TCGGTTA | 30 | 2.1649703E-6 | 44.999996 | 29 |