FastQCFastQC Report
Sat 18 Jun 2016
SRR3550269_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550269_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences898097
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGCT48400.538917288444344Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGC38920.4333607616994601TruSeq Adapter, Index 15 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGC27550.30675973753391894TruSeq Adapter, Index 15 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGC20420.22736964938085752TruSeq Adapter, Index 15 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCC17990.20031243841144106No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17890.19919897293944863No Hit
ACTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGC12200.13584278758307844No Hit
GCCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTG10430.11613444872881215TruSeq Adapter, Index 15 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCCG207.032462E-445.010
ATTACGC207.032462E-445.045
ATTCGCG207.032462E-445.01
ACGATAC207.032462E-445.022
GTATGCG253.8901213E-545.01
CGCTAGA207.032462E-445.033
ACGGAAC253.8901213E-545.01
CGAATTA207.032462E-445.025
CGAATAT700.045.014
TTAACGA207.032462E-445.022
AATTACG207.032462E-445.044
TTACGAG351.2115379E-745.01
TACAGCG253.8901213E-545.01
CGATACT207.032462E-445.010
CCCGCAA207.032462E-445.040
AAATGCG453.8380676E-1045.01
ACGAATT207.032462E-445.024
CCCGATC253.8901213E-545.041
TCGTTCA302.1649703E-644.99999616
TCGGTTA302.1649703E-644.99999629