##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550269_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	898097
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.38063371773873	33.0	31.0	34.0	30.0	34.0
2	32.56310287196149	34.0	31.0	34.0	31.0	34.0
3	32.59413960852781	34.0	31.0	34.0	31.0	34.0
4	36.04754942951597	37.0	35.0	37.0	35.0	37.0
5	36.08510439295533	37.0	35.0	37.0	35.0	37.0
6	36.120089478085326	37.0	35.0	37.0	35.0	37.0
7	36.184509022967454	37.0	36.0	37.0	35.0	37.0
8	36.120259838302545	37.0	36.0	37.0	35.0	37.0
9	37.61837195759478	39.0	38.0	39.0	35.0	39.0
10	37.618269518771356	39.0	37.0	39.0	35.0	39.0
11	37.59496802683897	39.0	37.0	39.0	35.0	39.0
12	37.42727901329144	39.0	37.0	39.0	35.0	39.0
13	37.36628226127022	39.0	37.0	39.0	35.0	39.0
14	38.60149404797032	40.0	38.0	41.0	35.0	41.0
15	38.69938102454412	40.0	38.0	41.0	35.0	41.0
16	38.75716208828222	40.0	38.0	41.0	35.0	41.0
17	38.742134758272215	40.0	38.0	41.0	35.0	41.0
18	38.688102732778304	40.0	38.0	41.0	35.0	41.0
19	38.62010005600731	40.0	38.0	41.0	35.0	41.0
20	38.55390453369736	40.0	38.0	41.0	35.0	41.0
21	38.4897767167689	40.0	37.0	41.0	34.0	41.0
22	38.45225627075917	40.0	37.0	41.0	34.0	41.0
23	38.34817731269562	40.0	37.0	41.0	34.0	41.0
24	38.31006450305479	40.0	37.0	41.0	34.0	41.0
25	38.30629096857021	40.0	37.0	41.0	34.0	41.0
26	38.26555706120831	40.0	37.0	41.0	34.0	41.0
27	38.24149395889308	40.0	37.0	41.0	34.0	41.0
28	38.16889935051559	40.0	37.0	41.0	34.0	41.0
29	38.09865972161136	40.0	36.0	41.0	34.0	41.0
30	38.04878203579346	40.0	36.0	41.0	34.0	41.0
31	37.910712317266395	40.0	36.0	41.0	34.0	41.0
32	37.74086874803056	40.0	36.0	41.0	33.0	41.0
33	37.66836098995988	40.0	36.0	41.0	33.0	41.0
34	37.60479881349119	40.0	36.0	41.0	33.0	41.0
35	37.489515052382984	40.0	35.0	41.0	33.0	41.0
36	37.44986009306344	40.0	35.0	41.0	33.0	41.0
37	37.35617756211189	40.0	35.0	41.0	33.0	41.0
38	37.22135136850474	39.0	35.0	41.0	33.0	41.0
39	37.13649861874608	39.0	35.0	41.0	33.0	41.0
40	37.07220712239324	39.0	35.0	41.0	32.0	41.0
41	37.03683566474445	39.0	35.0	41.0	32.0	41.0
42	36.958665934748694	39.0	35.0	41.0	32.0	41.0
43	36.87737516103494	39.0	35.0	41.0	32.0	41.0
44	36.778414803746145	39.0	35.0	41.0	32.0	41.0
45	36.62629537789348	39.0	35.0	41.0	31.0	41.0
46	36.54329988854211	39.0	35.0	41.0	31.0	41.0
47	36.45419592761138	39.0	35.0	41.0	31.0	41.0
48	36.45297779638502	39.0	35.0	41.0	31.0	41.0
49	36.36016599543257	38.0	35.0	41.0	31.0	41.0
50	36.23457265751917	38.0	35.0	40.0	31.0	41.0
51	35.2231418209837	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	8.0
9	7.0
10	18.0
11	15.0
12	10.0
13	17.0
14	7.0
15	34.0
16	42.0
17	75.0
18	140.0
19	283.0
20	516.0
21	767.0
22	1211.0
23	2034.0
24	3083.0
25	4302.0
26	5403.0
27	6619.0
28	7513.0
29	8858.0
30	10853.0
31	13908.0
32	17935.0
33	25161.0
34	49675.0
35	76339.0
36	61747.0
37	93548.0
38	174680.0
39	332881.0
40	406.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.178186766017475	20.495892982606556	25.510607428818936	10.81531282255703
2	31.693235808604193	24.89118658674954	25.353274757626405	18.062302847019865
3	32.06847367266565	24.125122342018734	26.37810837804825	17.428295607267366
4	29.126920588756004	26.162541462670514	25.40505090207405	19.30548704649943
5	25.693549805867296	29.887862892315642	24.495572304550624	19.923014997266442
6	26.483776251340334	34.615191900206774	23.797429453611358	15.103602394841536
7	78.76810634040645	6.830553937937661	8.919192470301091	5.482147251354809
8	78.21916786271416	6.279722568942998	9.481603880204476	6.019505688138364
9	71.71998124924144	8.671335056235574	11.807855944291095	7.80082775023188
10	38.39306890012994	28.233698587123662	18.212175299550047	15.161057213196347
11	32.15042473140429	24.45860525088047	23.56259958556815	19.828370432147086
12	29.311310470917952	22.67683780259816	28.364419433535577	19.64743229294831
13	25.583094031045643	24.528085496332803	29.63154314066298	20.257277331958576
14	19.88382101265231	28.45783918663574	29.047196460961345	22.611143339750605
15	18.958976591615382	25.921587534531348	34.35720195034612	20.762233923507146
16	22.335894675074073	25.115104493167216	31.047871221037372	21.501129610721335
17	21.499014026324552	25.201398067246632	28.499928181477056	24.79965972495176
18	23.257955432431018	24.828275787581966	29.836977520245583	22.076791259741434
19	23.7615758654132	26.297270784781603	27.668058127351504	22.273095222453698
20	26.662710152689524	25.933947001270464	26.524974473804054	20.87836837223596
21	24.099067249974112	27.43367364549709	28.065342607758403	20.401916496770394
22	22.099728648464474	25.895643789033922	26.85021773817305	25.154409824328557
23	22.863120576062496	26.505154788402592	27.547135777093118	23.084588858441794
24	23.46283307927763	24.128574084981913	28.34059127243494	24.068001563305522
25	21.20305490386896	27.363970706950365	26.12757864684995	25.305395742330727
26	21.74843029205086	28.156758123008984	27.01233831089515	23.08247327404501
27	23.788187690193823	27.281685608570122	26.871039542499307	22.05908715873675
28	20.664360308519015	27.762925385565257	27.865252862441363	23.70746144347437
29	21.850535075832568	27.76693386126443	27.12123523405601	23.261295828846997
30	25.34703935098325	26.670615757540666	26.19583408028309	21.786510811193
31	26.240484045709984	27.944420257500024	24.218430748571702	21.59666494821829
32	24.54601229043188	29.107212249901735	24.764808255678396	21.581967203987986
33	25.35505630238159	27.499368108344648	24.287354261288037	22.85822132798573
34	23.731400951122204	27.030821837730223	26.570403865061348	22.667373346086226
35	22.681959743769326	28.262537342848265	25.603025062994313	23.4524778503881
36	24.32309650293899	30.10465461971257	24.181575041448752	21.390673835899683
37	25.459165324012883	28.09095231361423	25.606031419768687	20.843850942604195
38	22.63374668883205	28.888973017391216	25.509716656441345	22.967563637335388
39	24.042280510902497	26.042509884789727	26.263755474074628	23.651454130233148
40	24.749999164900895	25.924927930947327	27.480995928056767	21.84407697609501
41	22.114092353053177	27.532103993221224	24.678403335051783	25.67540031867382
42	23.07345420372187	26.258744879450663	25.42943579591069	25.238365120916782
43	24.362401834100325	24.637761845324057	27.121346580603205	23.87848973997241
44	23.35193191826718	24.996409073852824	25.8990955319971	25.752563475882894
45	23.732069030405402	24.169438267804033	26.3413640174725	25.757128684318065
46	24.265641684584182	25.93895759589443	26.56995847887255	23.22544224064884
47	22.405597613620802	26.00821514825236	28.441359897650255	23.144827340476585
48	21.416172195208315	24.097508398313323	29.449491535992216	25.03682787048615
49	23.410054815905188	23.33634340165929	29.21020780606104	24.04339397637449
50	21.8751426627636	23.36095098859032	29.017689625953548	25.746216722692537
51	21.077901384817007	23.65546260593232	26.098405851483747	29.16823015776692
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	466.0
1	1029.0
2	1592.0
3	2619.0
4	3646.0
5	2694.0
6	1742.0
7	1748.0
8	1754.0
9	1957.5
10	2161.0
11	2279.0
12	2397.0
13	2401.5
14	2406.0
15	2472.0
16	2538.0
17	2429.0
18	2320.0
19	2473.0
20	2626.0
21	2767.0
22	2908.0
23	3134.5
24	3361.0
25	4205.5
26	6113.5
27	7177.0
28	8567.0
29	9957.0
30	10739.0
31	11521.0
32	13014.0
33	14507.0
34	17071.5
35	19636.0
36	20353.0
37	21070.0
38	23930.0
39	26790.0
40	30664.0
41	34538.0
42	41307.0
43	48076.0
44	52278.5
45	56481.0
46	66287.5
47	76094.0
48	86660.0
49	97226.0
50	95575.0
51	93924.0
52	82002.0
53	70080.0
54	61769.5
55	53459.0
56	48626.0
57	43793.0
58	42284.5
59	40776.0
60	39618.0
61	38460.0
62	35262.0
63	32064.0
64	27523.0
65	22982.0
66	18963.0
67	14944.0
68	12683.5
69	10423.0
70	8447.0
71	6471.0
72	5595.0
73	4719.0
74	4246.0
75	3042.0
76	2311.0
77	1589.0
78	867.0
79	791.0
80	715.0
81	458.5
82	202.0
83	135.5
84	69.0
85	43.5
86	18.0
87	10.5
88	3.0
89	3.0
90	3.0
91	1.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	898097.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.84391375331684
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.2536962294819	18.590402486826157
2	12.696722403705046	6.8165944230972775
3	5.071640546396053	4.08428044245841
4	2.572242127781859	2.7619618332329767
5	1.5118436560655564	2.02919003559615
6	0.979386821100994	1.5774345214062138
7	0.6585199122242035	1.2374076210011804
8	0.5081268931209016	1.0912091595742663
9	0.42460487762688676	1.025825104282842
>10	5.056111583906126	33.58442739199391
>50	1.1685430610125793	20.15517795606489
>100	0.0918797257082043	4.37025683932735
>500	0.0033410809348437924	0.5031479322482465
>1k	0.0033410809348437924	2.1726842528901558
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGCT	4840	0.538917288444344	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGC	3892	0.4333607616994601	TruSeq Adapter, Index 15 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGC	2755	0.30675973753391894	TruSeq Adapter, Index 15 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGC	2042	0.22736964938085752	TruSeq Adapter, Index 15 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCC	1799	0.20031243841144106	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1789	0.19919897293944863	No Hit
ACTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGC	1220	0.13584278758307844	No Hit
GCCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTG	1043	0.11613444872881215	TruSeq Adapter, Index 15 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.453861887969785E-4	0.0	0.0	0.5638589150169748	0.0
2	4.453861887969785E-4	0.0	0.0	1.7253147488522955	0.0
3	4.453861887969785E-4	0.0	0.0	2.331262658710585	0.0
4	4.453861887969785E-4	0.0	0.0	3.0102539035315785	0.0
5	4.453861887969785E-4	0.0	0.0	4.773203785337219	0.0
6	4.453861887969785E-4	0.0	0.0	5.791356612927111	0.0
7	4.453861887969785E-4	0.0	0.0	6.772319693752457	0.0
8	4.453861887969785E-4	0.0	0.0	8.364909358343253	0.0
9	4.453861887969785E-4	0.0	0.0	8.940459660816147	0.0
10	4.453861887969785E-4	0.0	0.0	10.282408247661444	0.0
11	4.453861887969785E-4	0.0	0.0	12.416476171282167	0.0
12	4.453861887969785E-4	0.0	0.0	14.068970278266157	0.0
13	5.567327359962232E-4	0.0	0.0	14.729923382440873	0.0
14	5.567327359962232E-4	0.0	0.0	15.00160895760703	0.0
15	5.567327359962232E-4	0.0	0.0	15.512355569609964	0.0
16	5.567327359962232E-4	0.0	0.0	16.513249682383975	0.0
17	5.567327359962232E-4	0.0	0.0	17.6740374369361	0.0
18	5.567327359962232E-4	0.0	0.0	18.93113995481557	0.0
19	5.567327359962232E-4	0.0	0.0	19.70032190286795	0.0
20	5.567327359962232E-4	0.0	0.0	20.444896263989303	0.0
21	5.567327359962232E-4	0.0	0.0	21.33533460194166	0.0
22	5.567327359962232E-4	0.0	0.0	22.207400759606145	0.0
23	5.567327359962232E-4	0.0	0.0	23.024350376407003	0.0
24	5.567327359962232E-4	0.0	0.0	23.684190015109728	0.0
25	5.567327359962232E-4	0.0	0.0	24.28334578558886	0.0
26	6.680792831954678E-4	0.0	0.0	24.83495658041392	0.0
27	6.680792831954678E-4	0.0	0.0	25.35561303511759	0.0
28	6.680792831954678E-4	0.0	0.0	25.879609886237233	0.0
29	6.680792831954678E-4	0.0	0.0	26.442578028876614	0.0
30	6.680792831954678E-4	0.0	0.0	27.083600101102665	0.0
31	6.680792831954678E-4	0.0	0.0	27.66527446367152	0.0
32	6.680792831954678E-4	0.0	0.0	28.21476967409979	0.0
33	6.680792831954678E-4	0.0	0.0	28.745335971504193	0.0
34	6.680792831954678E-4	0.0	0.0	29.27913131877737	0.0
35	6.680792831954678E-4	0.0	0.0	29.81882803305211	0.0
36	6.680792831954678E-4	0.0	0.0	30.369436708952374	0.0
37	6.680792831954678E-4	0.0	0.0	30.89955762016798	0.0
38	6.680792831954678E-4	0.0	0.0	31.434800472554745	0.0
39	6.680792831954678E-4	0.0	0.0	32.00712172515886	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCCG	20	7.032462E-4	45.0	10
ATTACGC	20	7.032462E-4	45.0	45
ATTCGCG	20	7.032462E-4	45.0	1
ACGATAC	20	7.032462E-4	45.0	22
GTATGCG	25	3.8901213E-5	45.0	1
CGCTAGA	20	7.032462E-4	45.0	33
ACGGAAC	25	3.8901213E-5	45.0	1
CGAATTA	20	7.032462E-4	45.0	25
CGAATAT	70	0.0	45.0	14
TTAACGA	20	7.032462E-4	45.0	22
AATTACG	20	7.032462E-4	45.0	44
TTACGAG	35	1.2115379E-7	45.0	1
TACAGCG	25	3.8901213E-5	45.0	1
CGATACT	20	7.032462E-4	45.0	10
CCCGCAA	20	7.032462E-4	45.0	40
AAATGCG	45	3.8380676E-10	45.0	1
ACGAATT	20	7.032462E-4	45.0	24
CCCGATC	25	3.8901213E-5	45.0	41
TCGTTCA	30	2.1649703E-6	44.999996	16
TCGGTTA	30	2.1649703E-6	44.999996	29
>>END_MODULE