FastQCFastQC Report
Sat 18 Jun 2016
SRR3550264_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550264_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1077745
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC62850.5831620652380665TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGCT54180.5027163197231256TruSeq Adapter, Index 22 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC48550.4504776176182678TruSeq Adapter, Index 20 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC25310.23484219365434308TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18140.16831439719043No Hit
ACTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC16830.15615938835253237TruSeq Adapter, Index 22 (95% over 22bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA16690.15486037977443645No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCC16630.15430366181239533No Hit
GCCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTG12750.11830256693373664No Hit
AGCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTG12120.11245702833230496TruSeq Adapter, Index 22 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACAA253.8906423E-545.00000434
GCAAACG253.8906423E-545.0000041
TATAGCG700.045.0000041
ATCGATG253.8906423E-545.0000041
TAGCCGT207.033092E-445.044
CATTACG207.033092E-445.042
CTACGCG207.033092E-445.01
ACTCGCA453.8562575E-1045.012
CGTAAGA207.033092E-445.022
TAAGCGT207.033092E-445.044
TATTGCG700.041.7857171
TAGCGAG650.041.5384641
GCGATCA603.6379788E-1241.2500049
TACGTAG603.6379788E-1241.2500041
TTGCGAG556.184564E-1140.9090921
GCGATAT451.9281288E-840.09
AATAGCG451.9281288E-840.01
CGAGATA1300.039.80769319
TTGGACG850.039.7058831
AACGGGA2950.039.661024
Produced by FastQC (version 0.11.5)