##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550264_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1077745
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.23190643426785	33.0	31.0	34.0	30.0	34.0
2	32.409677613906815	34.0	31.0	34.0	30.0	34.0
3	32.44332935898566	34.0	31.0	34.0	30.0	34.0
4	35.92891361129024	37.0	35.0	37.0	35.0	37.0
5	35.97779344835745	37.0	35.0	37.0	35.0	37.0
6	36.015279124468215	37.0	35.0	37.0	35.0	37.0
7	36.103387164867385	37.0	35.0	37.0	35.0	37.0
8	36.04017926318378	37.0	35.0	37.0	35.0	37.0
9	37.484150703552324	39.0	38.0	39.0	35.0	39.0
10	37.452076326032596	39.0	37.0	39.0	35.0	39.0
11	37.45072071779503	39.0	37.0	39.0	35.0	39.0
12	37.32511957837893	39.0	37.0	39.0	35.0	39.0
13	37.28490041707454	39.0	37.0	39.0	34.0	39.0
14	38.49936394972837	40.0	38.0	41.0	34.0	41.0
15	38.55777015898937	40.0	38.0	41.0	35.0	41.0
16	38.62991431182701	40.0	38.0	41.0	35.0	41.0
17	38.62029515330621	40.0	38.0	41.0	35.0	41.0
18	38.5854520317886	40.0	38.0	41.0	35.0	41.0
19	38.53489183433929	40.0	38.0	41.0	34.0	41.0
20	38.44413938362043	40.0	37.0	41.0	34.0	41.0
21	38.35959804963141	40.0	37.0	41.0	34.0	41.0
22	38.30360242914604	40.0	37.0	41.0	34.0	41.0
23	38.18724651935291	40.0	37.0	41.0	34.0	41.0
24	38.11588362738867	40.0	37.0	41.0	34.0	41.0
25	38.12453316879225	40.0	37.0	41.0	34.0	41.0
26	38.09960241058877	40.0	37.0	41.0	34.0	41.0
27	38.06203786610005	40.0	37.0	41.0	34.0	41.0
28	38.004947366956	40.0	37.0	41.0	34.0	41.0
29	37.998341908336386	40.0	37.0	41.0	34.0	41.0
30	37.90786132155566	40.0	36.0	41.0	34.0	41.0
31	37.77570390027326	40.0	36.0	41.0	33.0	41.0
32	37.72011932321653	40.0	36.0	41.0	33.0	41.0
33	37.66901818148078	40.0	36.0	41.0	33.0	41.0
34	37.576202162849285	40.0	36.0	41.0	33.0	41.0
35	37.51599961029743	40.0	36.0	41.0	33.0	41.0
36	37.440111529165065	40.0	36.0	41.0	33.0	41.0
37	37.35885761474189	40.0	35.0	41.0	33.0	41.0
38	37.2967900570172	39.0	35.0	41.0	33.0	41.0
39	37.20185572654014	39.0	35.0	41.0	32.0	41.0
40	37.109370955096054	39.0	35.0	41.0	32.0	41.0
41	37.035914803594544	39.0	35.0	41.0	32.0	41.0
42	36.9946805598727	39.0	35.0	41.0	32.0	41.0
43	36.95950990262075	39.0	35.0	41.0	32.0	41.0
44	36.85139249080255	39.0	35.0	41.0	31.0	41.0
45	36.70705500837396	39.0	35.0	41.0	31.0	41.0
46	36.62979647319171	39.0	35.0	41.0	31.0	41.0
47	36.57266793165359	39.0	35.0	41.0	31.0	41.0
48	36.579107302747865	39.0	35.0	41.0	31.0	41.0
49	36.487588436967926	39.0	35.0	41.0	31.0	41.0
50	36.34244835281073	39.0	35.0	40.0	31.0	41.0
51	35.37270040686804	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	18.0
10	18.0
11	19.0
12	17.0
13	15.0
14	23.0
15	39.0
16	60.0
17	96.0
18	196.0
19	329.0
20	619.0
21	953.0
22	1576.0
23	2440.0
24	3818.0
25	5000.0
26	6598.0
27	8030.0
28	9674.0
29	11531.0
30	14140.0
31	18011.0
32	23291.0
33	31962.0
34	59273.0
35	86409.0
36	76687.0
37	115643.0
38	210184.0
39	390628.0
40	441.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.99240079981814	21.951760388589136	24.986058854367222	11.069779957225503
2	31.139555275134654	24.8368584405402	26.09114400901883	17.93244227530631
3	31.941321926800864	24.436949371140667	26.548580601162612	17.073148100895853
4	28.597209915146905	26.313552834854253	26.548395028508597	18.54084222149024
5	25.128300293668726	29.94186936613021	24.747226848651582	20.182603491549486
6	25.587592612352644	34.149358150582934	24.424144858013726	15.838904379050703
7	77.2213278651258	7.1916826336471065	9.613405768525949	5.973583732701149
8	75.5923711081935	6.752803306904695	10.50805153352602	7.14677405137579
9	68.88902291358346	9.02625389122659	13.188184589118949	8.89653860607101
10	38.292546010419905	26.00448157959443	19.75736375487708	15.945608655108584
11	29.783668678583524	24.879818509944375	24.67893611197454	20.65757669949756
12	26.71336911792678	21.843293172318127	30.57476490264395	20.868572807111143
13	23.68491619075013	23.87828289623241	32.00617956937866	20.4306213436388
14	20.00436095736932	27.587230745677317	29.879145809073577	22.529262487879787
15	19.067497413581137	25.17682754269331	35.20591605620996	20.5497589875156
16	20.607286510259847	25.628140237254637	31.523041164653975	22.241532087831537
17	20.206449577590245	26.265025585829672	28.459236646887714	25.06928818969237
18	21.470106565096568	25.849713986146998	30.603899809324098	22.076279639432332
19	22.736547142413094	27.085256716570242	27.60096312207433	22.577233018942326
20	24.025163651884256	27.054358869676964	27.60699423332978	21.313483245108998
21	22.806879178284287	27.717224389813914	28.697604720968318	20.778291710933477
22	20.217676723158075	26.138557822119335	26.661779920110973	26.981985534611617
23	21.53487142134735	26.332202886582635	27.707667398132212	24.425258293937805
24	22.99551378108922	24.594964486033337	28.661974771397684	23.747546961479756
25	21.403300409651635	26.483630172257815	26.46256767602726	25.65050174206329
26	21.557418498810016	27.172754222937705	27.083493776357116	24.18633350189516
27	22.977049302014855	26.139300112735388	26.528074822894098	24.35557576235566
28	19.98311288848475	26.078432282218895	27.8754250773606	26.06302975193576
29	23.64130661705691	24.00094642053547	27.301820003804238	25.05592695860338
30	24.752701241944987	24.42572222557284	25.60828396327517	25.213292569207002
31	23.034020106797062	25.56040621853964	25.321945358132027	26.083628316531275
32	23.86649903270254	25.31591424687658	26.9952539793736	23.822332741047276
33	23.577840769384224	24.10997035476852	27.010285364348707	25.301903511498548
34	21.62301843200386	22.773476100561822	30.456833481018236	25.14667198641608
35	22.711123688813217	22.38535089469216	30.040408445411483	24.86311697108314
36	23.30903878004537	23.908252879855624	28.64981976255979	24.132888577539212
37	20.58455386014317	24.47564126950253	31.744058195584294	23.195746674770007
38	20.80093157472315	25.211436842666863	29.84629944931315	24.141332133296835
39	24.243768238312402	22.159601761084485	31.315292578485632	22.281337422117478
40	23.801177458489718	21.56317125108444	32.33325137207781	22.30239991834803
41	20.07283726670038	23.46176507429865	30.20111436378735	26.26428329521362
42	21.61030670520392	22.68625695317538	30.65892210123916	25.044514240381538
43	22.875355487615344	21.588501918357313	30.209557919544977	25.326584674482366
44	22.241810446812558	22.292564567685304	28.323861395784718	27.14176358971742
45	22.588460164510156	21.09246621417868	28.78185470589054	27.537218915420624
46	23.40720671401862	22.629007789412153	29.634561051083512	24.329224445485714
47	20.39090879567987	22.27530631086203	32.71293302218985	24.62085187126825
48	20.09046666883168	20.902810961776673	32.042551809565346	26.964170559826307
49	21.939234234443212	19.93189483597697	33.28765153167029	24.841219397909526
50	20.927492124760494	19.915842801404786	32.3994080232337	26.757257050601023
51	20.376526914993807	20.27288458772715	29.347016223689277	30.00357227358976
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	576.0
1	1070.5
2	1565.0
3	2537.5
4	3510.0
5	2557.0
6	1604.0
7	1637.5
8	1671.0
9	1787.5
10	1904.0
11	2058.5
12	2213.0
13	2334.0
14	2455.0
15	2394.5
16	2334.0
17	2331.0
18	2328.0
19	2709.5
20	3091.0
21	3109.0
22	3127.0
23	3411.5
24	3696.0
25	5590.0
26	8645.5
27	9807.0
28	11003.0
29	12199.0
30	14157.0
31	16115.0
32	18291.0
33	20467.0
34	21571.0
35	22675.0
36	24176.5
37	25678.0
38	29058.5
39	32439.0
40	37142.0
41	41845.0
42	48060.5
43	54276.0
44	62319.5
45	70363.0
46	84720.0
47	99077.0
48	114820.0
49	130563.0
50	125715.0
51	120867.0
52	100981.5
53	81096.0
54	70190.0
55	59284.0
56	53096.5
57	46909.0
58	44703.0
59	42497.0
60	41828.5
61	41160.0
62	37105.0
63	33050.0
64	29010.5
65	24971.0
66	21141.5
67	17312.0
68	15306.0
69	13300.0
70	10953.5
71	8607.0
72	7476.5
73	6346.0
74	4954.0
75	3073.0
76	2584.0
77	2191.5
78	1799.0
79	1273.5
80	748.0
81	515.0
82	282.0
83	239.0
84	196.0
85	116.0
86	36.0
87	42.5
88	49.0
89	33.5
90	18.0
91	11.5
92	5.0
93	3.5
94	2.0
95	2.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1077745.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.95436720228513
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.09445333531347	18.35442899638909
2	12.866729045648167	6.936290787774169
3	5.263985708042986	4.256622111665146
4	2.7115305640205953	2.9235036201132174
5	1.541534719637068	2.0775546444084596
6	0.9972704479141108	1.6128476311838584
7	0.70399767516856	1.328306829223389
8	0.5785039317030901	1.2474565922472616
9	0.44674231258765695	1.0837490704457176
>10	6.025680369883504	40.39964033223704
>50	0.6706418797974292	11.082286581961833
>100	0.08782207930431045	4.618918227108983
>500	0.007636702548196284	1.420687403484182
>1k	0.002776982744798649	1.5627187179262914
>5k	6.942456861996622E-4	1.0949884538313608
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC	6285	0.5831620652380665	TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGCT	5418	0.5027163197231256	TruSeq Adapter, Index 22 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC	4855	0.4504776176182678	TruSeq Adapter, Index 20 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC	2531	0.23484219365434308	TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1814	0.16831439719043	No Hit
ACTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC	1683	0.15615938835253237	TruSeq Adapter, Index 22 (95% over 22bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1669	0.15486037977443645	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCC	1663	0.15430366181239533	No Hit
GCCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTG	1275	0.11830256693373664	No Hit
AGCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTG	1212	0.11245702833230496	TruSeq Adapter, Index 22 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8557265401370455E-4	0.0	0.0	0.5223870210485783	0.0
2	1.8557265401370455E-4	0.0	0.0	2.0103085609304614	0.0
3	1.8557265401370455E-4	0.0	0.0	2.7663315533822934	0.0
4	1.8557265401370455E-4	0.0	0.0	3.663018617576514	0.0
5	1.8557265401370455E-4	0.0	0.0	6.029069956251247	0.0
6	1.8557265401370455E-4	0.0	0.0	7.396554843678236	0.0
7	1.8557265401370455E-4	0.0	0.0	8.755039457385559	0.0
8	1.8557265401370455E-4	0.0	0.0	10.92688901363495	0.0
9	1.8557265401370455E-4	0.0	0.0	11.724480280585853	0.0
10	1.8557265401370455E-4	0.0	0.0	13.385726679316535	0.0
11	1.8557265401370455E-4	0.0	0.0	15.669290973282177	0.0
12	1.8557265401370455E-4	0.0	0.0	17.3567959025558	0.0
13	3.711453080274091E-4	0.0	0.0	18.050002551623994	0.0
14	4.6393163503426134E-4	0.0	0.0	18.33188741307081	0.0
15	4.6393163503426134E-4	0.0	0.0	18.852047562271224	0.0
16	4.6393163503426134E-4	0.0	0.0	19.901182561737702	0.0
17	4.6393163503426134E-4	0.0	0.0	21.105456299959638	0.0
18	4.6393163503426134E-4	0.0	0.0	22.472477255751592	0.0
19	5.567179620411136E-4	0.0	0.0	23.262738402868955	0.0
20	5.567179620411136E-4	0.0	0.0	24.02748331005943	0.0
21	5.567179620411136E-4	0.0	0.0	24.94031519515284	0.0
22	5.567179620411136E-4	0.0	0.0	25.878477747519124	0.0
23	7.422906160548182E-4	0.0	0.0	26.779711341736682	0.0
24	7.422906160548182E-4	0.0	0.0	27.503537478717135	0.0
25	7.422906160548182E-4	0.0	0.0	28.138195955444004	0.0
26	7.422906160548182E-4	0.0	0.0	28.721543593336087	0.0
27	7.422906160548182E-4	0.0	0.0	29.261931161823995	0.0
28	7.422906160548182E-4	0.0	0.0	29.83627852599641	0.0
29	7.422906160548182E-4	0.0	0.0	30.42222418104468	0.0
30	7.422906160548182E-4	0.0	0.0	31.071542897438633	0.0
31	7.422906160548182E-4	0.0	0.0	31.700170262910056	0.0
32	7.422906160548182E-4	0.0	0.0	32.308384636439975	0.0
33	7.422906160548182E-4	0.0	0.0	32.86890683788837	0.0
34	7.422906160548182E-4	0.0	0.0	33.42506808196744	0.0
35	7.422906160548182E-4	0.0	0.0	33.990600745074204	0.0
36	7.422906160548182E-4	0.0	0.0	34.55604062185397	0.0
37	9.278632700685227E-4	0.0	0.0	35.14365643078836	0.0
38	0.001020649597075375	0.0	0.0	35.693879349938996	0.0
39	0.001020649597075375	0.0	0.0	36.252081893212214	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACAA	25	3.8906423E-5	45.000004	34
GCAAACG	25	3.8906423E-5	45.000004	1
TATAGCG	70	0.0	45.000004	1
ATCGATG	25	3.8906423E-5	45.000004	1
TAGCCGT	20	7.033092E-4	45.0	44
CATTACG	20	7.033092E-4	45.0	42
CTACGCG	20	7.033092E-4	45.0	1
ACTCGCA	45	3.8562575E-10	45.0	12
CGTAAGA	20	7.033092E-4	45.0	22
TAAGCGT	20	7.033092E-4	45.0	44
TATTGCG	70	0.0	41.785717	1
TAGCGAG	65	0.0	41.538464	1
GCGATCA	60	3.6379788E-12	41.250004	9
TACGTAG	60	3.6379788E-12	41.250004	1
TTGCGAG	55	6.184564E-11	40.909092	1
GCGATAT	45	1.9281288E-8	40.0	9
AATAGCG	45	1.9281288E-8	40.0	1
CGAGATA	130	0.0	39.807693	19
TTGGACG	85	0.0	39.705883	1
AACGGGA	295	0.0	39.66102	4
>>END_MODULE