SRR3550260_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550260_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1127269
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3579	0.31749298525906416	No Hit
CTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGCT	3249	0.28821869491665253	TruSeq Adapter, Index 1 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGC	3218	0.2854686858238806	RNA PCR Primer, Index 33 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCC	2098	0.18611351860115022	No Hit
CCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGC	1728	0.15329082942935537	Illumina Paired End PCR Primer 2 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGC	1390	0.12330685932106711	RNA PCR Primer, Index 33 (95% over 24bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGAGT	30	2.1654632E-6	45.000004	19
TCGGTCA	20	7.033229E-4	45.0	39
CGTTTTT	2850	0.0	41.44737	1
TATTGCG	60	3.6379788E-12	41.250004	1
GTCAACG	50	1.0822987E-9	40.5	1
TACGTAG	50	1.0822987E-9	40.5	1
TCGTAGT	40	3.4581353E-7	39.375	1
GGCGATA	400	0.0	38.8125	8
GTCGACG	35	6.2481813E-6	38.57143	1
TACGACG	35	6.2481813E-6	38.57143	1
AACGCCA	35	6.2481813E-6	38.57143	15
CACCGTC	65	9.094947E-12	38.076927	26
GCGATAT	95	0.0	37.894737	9
TACGGGA	310	0.0	37.74194	4
GTAATCG	30	1.13990776E-4	37.500004	1
GTTAACG	30	1.13990776E-4	37.500004	1
AACGACT	30	1.13990776E-4	37.500004	35
CGTAAAG	30	1.13990776E-4	37.500004	20
AGTAACG	30	1.13990776E-4	37.500004	1
TCTACGG	85	0.0	37.058823	2