FastQCFastQC Report
Sat 18 Jun 2016
SRR3550257_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550257_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1178878
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37010.3139425792999785No Hit
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC18230.15463856310831145TruSeq Adapter, Index 15 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC14490.12291348214149386TruSeq Adapter, Index 15 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT12320.10450614906716386TruSeq Adapter, Index 21 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACGCG453.8562575E-1045.01
CCGGTAT207.033361E-445.042
TACCGGT207.033361E-445.040
TAATCCG207.033361E-445.01
CATACGA850.042.3529418
TACGCGG1150.041.086962
AGTTACG501.0822987E-940.51
ATTAACG403.4583172E-739.37516
TCTAGCG403.4583172E-739.3751
TTACGCG403.4583172E-739.3751
CACGACC700.038.5714327
CGTTTTT20800.038.076921
GCGTAAG950.037.8947371
ACCTCGC301.1399397E-437.49999617
ACGATTG601.5643309E-1037.4999961
AATGCGA601.5643309E-1037.49999625
TATAGCG601.5643309E-1037.4999961
GTACGAC301.1399397E-437.49999638
CGGTCTA552.750312E-936.81818431
TCCGAAT250.002107130836.022
Produced by FastQC (version 0.11.5)