##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550257_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1178878
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.49771307972496	34.0	31.0	34.0	31.0	34.0
2	32.68002032441016	34.0	31.0	34.0	31.0	34.0
3	32.68782689981491	34.0	31.0	34.0	31.0	34.0
4	36.120298283622226	37.0	35.0	37.0	35.0	37.0
5	36.14410821136708	37.0	35.0	37.0	35.0	37.0
6	36.181212135606906	37.0	36.0	37.0	35.0	37.0
7	36.23432874309301	37.0	37.0	37.0	35.0	37.0
8	36.19232948617245	37.0	37.0	37.0	35.0	37.0
9	37.73029694336479	39.0	38.0	39.0	35.0	39.0
10	37.61562943748208	39.0	37.0	39.0	35.0	39.0
11	37.5683938456736	39.0	37.0	39.0	35.0	39.0
12	37.423966686968456	39.0	37.0	39.0	35.0	39.0
13	37.40283303276505	39.0	37.0	39.0	35.0	39.0
14	38.69435259628223	40.0	38.0	41.0	35.0	41.0
15	38.76654327250148	40.0	38.0	41.0	35.0	41.0
16	38.82755637139721	40.0	38.0	41.0	35.0	41.0
17	38.82346943449619	40.0	38.0	41.0	35.0	41.0
18	38.754613284835244	40.0	38.0	41.0	35.0	41.0
19	38.68542461560908	40.0	38.0	41.0	35.0	41.0
20	38.58662304326656	40.0	38.0	41.0	35.0	41.0
21	38.53964023418878	40.0	38.0	41.0	35.0	41.0
22	38.486550771156985	40.0	37.0	41.0	35.0	41.0
23	38.384287432626614	40.0	37.0	41.0	34.0	41.0
24	38.340063178717394	40.0	37.0	41.0	34.0	41.0
25	38.35070041174744	40.0	37.0	41.0	34.0	41.0
26	38.3083593043555	40.0	37.0	41.0	34.0	41.0
27	38.251110801965936	40.0	37.0	41.0	34.0	41.0
28	38.18779975536061	40.0	37.0	41.0	34.0	41.0
29	38.13904916369633	40.0	37.0	41.0	34.0	41.0
30	38.07218897969086	40.0	37.0	41.0	34.0	41.0
31	37.96139210333894	40.0	36.0	41.0	34.0	41.0
32	37.849653653728375	40.0	36.0	41.0	34.0	41.0
33	37.73455777442619	40.0	36.0	41.0	33.0	41.0
34	37.662201686688526	40.0	36.0	41.0	33.0	41.0
35	37.545570449189825	40.0	36.0	41.0	33.0	41.0
36	37.46245497837774	40.0	36.0	41.0	33.0	41.0
37	37.39151040226385	40.0	35.0	41.0	33.0	41.0
38	37.30346736473155	40.0	35.0	41.0	33.0	41.0
39	37.23557060187738	40.0	35.0	41.0	33.0	41.0
40	37.147008426656534	40.0	35.0	41.0	32.0	41.0
41	37.078354163874465	39.0	35.0	41.0	32.0	41.0
42	36.97865767280414	39.0	35.0	41.0	32.0	41.0
43	36.94168268472225	39.0	35.0	41.0	32.0	41.0
44	36.86764448908199	39.0	35.0	41.0	32.0	41.0
45	36.74651829960352	39.0	35.0	41.0	31.0	41.0
46	36.67403073091533	39.0	35.0	41.0	31.0	41.0
47	36.597198353010235	39.0	35.0	41.0	31.0	41.0
48	36.533858465422206	39.0	35.0	41.0	31.0	41.0
49	36.44529629020136	39.0	35.0	41.0	31.0	41.0
50	36.32186282210712	39.0	35.0	41.0	31.0	41.0
51	35.39319335843064	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	21.0
10	28.0
11	29.0
12	19.0
13	25.0
14	39.0
15	38.0
16	79.0
17	130.0
18	219.0
19	431.0
20	714.0
21	1246.0
22	1828.0
23	2898.0
24	4188.0
25	6420.0
26	8067.0
27	9186.0
28	10257.0
29	11616.0
30	13696.0
31	17333.0
32	22478.0
33	31005.0
34	61170.0
35	94213.0
36	76558.0
37	115795.0
38	211940.0
39	476541.0
40	664.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.63282544928313	21.321205417354467	24.86822215700013	11.17774697636227
2	32.61575837364002	24.498718272798374	25.347661081129687	17.537862272431923
3	31.416821757637347	23.724677193059843	27.59547637669038	17.263024672612435
4	28.229129731829754	26.213314694141378	27.003981752140593	18.55357382188827
5	25.389310853201096	30.625645741119946	25.348254866067567	18.636788539611395
6	27.02450974570736	35.30170212693765	23.472912379398036	14.200875747956957
7	81.16845000076344	6.3388238647256125	8.01558770288359	4.477138431627361
8	82.84283869916989	5.503283630706486	7.516553875803942	4.137323794319683
9	77.14869562414431	7.038132868710758	9.465610521190488	6.347560985954441
10	42.14414044540656	24.76719389114056	17.652717244702167	15.435948418750712
11	32.737399459486056	26.202117606741325	21.560331094481363	19.500151839291256
12	27.750454245477478	23.726034415775	27.72780559141828	20.79570574732924
13	25.117781483749802	24.986555012477965	29.45529562855529	20.440367875216943
14	22.585543202943818	27.73985094301531	28.773206387768706	20.901399466272167
15	21.307124231684703	26.721255295289247	31.882858107454716	20.08876236557133
16	23.460782201381313	25.04805416675856	30.702583303785463	20.78858032807466
17	24.251788565059318	25.01005193073414	27.832905525423325	22.905253978783215
18	24.1458403668573	25.88037099682919	28.10036322672914	21.873425409584367
19	25.651424490065978	27.406652766444022	26.16224918948356	20.779673554006436
20	26.856807913965653	26.647625962991928	26.071569746827066	20.42399637621535
21	25.532413023230564	26.6518672839768	26.95113489266913	20.864584800123506
22	24.01054222743999	25.473204182281794	26.3320716817177	24.18418190856051
23	23.085170814961344	27.107894116269875	27.19797977398849	22.60895529478029
24	23.559265674649964	25.779851689487803	28.07177672329113	22.589105912571107
25	24.012323582253632	26.720576683931668	26.47254423273655	22.794555501078143
26	23.18925283193002	28.251269427370772	26.328763451349502	22.230714289349702
27	23.84699689026345	27.54059368314618	26.969372572904067	21.6430368536863
28	22.104747056099107	27.427774544948676	28.12462358276259	22.342854816189632
29	23.847251369522546	27.08100414122581	26.972850456111658	22.098894033139985
30	24.591009417429117	27.112559569353234	26.038741922404185	22.257689090813468
31	24.589143236195774	27.929522817458636	25.12982683534683	22.351507110998764
32	24.788400496064902	28.64842672439387	25.514514648674417	21.04865813086681
33	24.17366343251804	29.226264295372378	24.826996517027208	21.773075755082374
34	24.06092912074023	27.51913259896274	26.70123626024067	21.71870202005636
35	24.03302122865979	27.633308959875407	26.151221754922897	22.182448056541897
36	24.51118775649389	29.519509228266198	25.292693561165784	20.67660945407413
37	23.86684627247264	29.28148629459537	26.824404221641256	20.027263211290737
38	23.418877950050813	28.867533366472188	26.131033067034927	21.582555616442075
39	24.800954806180115	27.592083319902482	25.405682352202685	22.201279521714714
40	24.50397751081961	26.24902661683397	28.157451407185473	21.08954446516094
41	22.800578176876655	26.65118867261922	27.256170697900885	23.292062452603236
42	23.60269680153502	27.12418078885177	26.6872399009906	22.585882508622607
43	23.77837231672828	26.432845468318177	27.115358841203246	22.673423373750296
44	23.502092667773937	26.808880986836638	26.451931412750092	23.23709493263934
45	23.245747227448472	26.76222645600308	26.235284736843	23.756741579705448
46	23.321157914559436	27.570028450781166	26.274729022002276	22.83408461265712
47	23.064388342135487	26.75094454218333	28.06702644378808	22.117640671893106
48	22.610821476013633	26.754083119712135	27.7337434407971	22.901351963477136
49	22.959118755291048	26.214417437597444	28.283588293275468	22.542875513836037
50	21.749833316085297	26.57823795167948	28.101381143765515	23.570547588469715
51	21.976574335936373	26.5259848771459	26.45905683200467	25.038383954913062
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	501.0
1	1092.5
2	1684.0
3	4068.5
4	6453.0
5	4491.5
6	2530.0
7	2592.0
8	2654.0
9	2731.0
10	2808.0
11	2804.0
12	2800.0
13	2883.5
14	2967.0
15	2939.5
16	2912.0
17	2792.0
18	2672.0
19	2771.0
20	2870.0
21	3269.5
22	3669.0
23	4207.5
24	4746.0
25	5969.0
26	7925.0
27	8658.0
28	10899.0
29	13140.0
30	15357.5
31	17575.0
32	20284.0
33	22993.0
34	25351.0
35	27709.0
36	31619.5
37	35530.0
38	39714.0
39	43898.0
40	48652.5
41	53407.0
42	61508.0
43	69609.0
44	82148.5
45	94688.0
46	101556.5
47	108425.0
48	106706.0
49	104987.0
50	93527.5
51	82068.0
52	76085.0
53	70102.0
54	67412.0
55	64722.0
56	61234.0
57	57746.0
58	58120.5
59	58495.0
60	56297.0
61	54099.0
62	49114.0
63	44129.0
64	37860.5
65	31592.0
66	27253.0
67	22914.0
68	19046.5
69	15179.0
70	13079.0
71	10979.0
72	9410.0
73	7841.0
74	6442.5
75	3912.0
76	2780.0
77	2266.5
78	1753.0
79	1567.0
80	1381.0
81	1016.5
82	652.0
83	429.5
84	207.0
85	144.0
86	81.0
87	53.0
88	25.0
89	15.0
90	5.0
91	4.5
92	4.0
93	2.0
94	0.0
95	1.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1178878.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.577132763278033
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.54887935028484	18.811694517325535
2	9.809080175824894	5.017762918854238
3	3.5259526610926017	2.705512779893968
4	1.7991089757908338	1.8406419651762929
5	1.1485248304012803	1.4687986034547464
6	0.8466163920116929	1.2992411914830306
7	0.6634070620390402	1.187763535130813
8	0.5391087381406159	1.1031084615412659
9	0.462102956768116	1.063734180800529
>10	6.224579762852862	38.588250119982995
>50	1.3534933488269816	22.405661413800388
>100	0.07346862494766397	3.0263175648824814
>500	0.004007379542592996	0.6812652450530947
>1k	0.0016697414760804149	0.8002475026206676
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3701	0.3139425792999785	No Hit
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	1823	0.15463856310831145	TruSeq Adapter, Index 15 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	1449	0.12291348214149386	TruSeq Adapter, Index 15 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT	1232	0.10450614906716386	TruSeq Adapter, Index 21 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.482641969737327E-5	0.0	0.0	0.11035917202628262	0.0
2	8.482641969737327E-5	0.0	0.0	0.5104853937387923	0.0
3	8.482641969737327E-5	0.0	0.0	0.7941449412068085	0.0
4	8.482641969737327E-5	0.0	0.0	1.1105474866780107	0.0
5	8.482641969737327E-5	0.0	0.0	2.0129309394186676	0.0
6	8.482641969737327E-5	0.0	0.0	2.6415795357959007	0.0
7	8.482641969737327E-5	0.0	0.0	3.194308486543985	0.0
8	8.482641969737327E-5	0.0	0.0	4.031375596117664	0.0
9	8.482641969737327E-5	0.0	0.0	4.382387320825395	0.0
10	8.482641969737327E-5	0.0	0.0	5.1397175958835435	0.0
11	8.482641969737327E-5	0.0	0.0	6.116239339439704	0.0
12	8.482641969737327E-5	0.0	0.0	6.878574373259998	0.0
13	8.482641969737327E-5	0.0	0.0	7.171480000475028	0.0
14	8.482641969737327E-5	0.0	0.0	7.298804456440785	0.0
15	8.482641969737327E-5	0.0	0.0	7.514178736052416	0.0
16	8.482641969737327E-5	0.0	0.0	7.955785076996941	0.0
17	8.482641969737327E-5	0.0	0.0	8.511228473175342	0.0
18	8.482641969737327E-5	0.0	0.0	9.128086197214639	0.0
19	8.482641969737327E-5	0.0	0.0	9.501916228820964	0.0
20	8.482641969737327E-5	0.0	0.0	9.86955393178938	0.0
21	1.6965283939474654E-4	0.0	0.0	10.3371171571613	0.0
22	1.6965283939474654E-4	0.0	0.0	10.826650425234842	0.0
23	2.544792590921198E-4	0.0	0.0	11.333488282926647	0.0
24	2.544792590921198E-4	0.0	0.0	11.739722006857368	0.0
25	3.3930567878949307E-4	0.0	0.0	12.114739608339455	0.0
26	3.3930567878949307E-4	0.0	0.0	12.454978377745618	0.0
27	3.3930567878949307E-4	0.0	0.0	12.80081569085181	0.0
28	5.089585181842396E-4	0.0	0.0	13.154287381730764	0.0
29	5.089585181842396E-4	0.0	0.0	13.531171164446194	0.0
30	5.089585181842396E-4	0.0	0.0	13.978376049090746	0.0
31	5.089585181842396E-4	0.0	0.0	14.372564421424439	0.0
32	5.089585181842396E-4	0.0	0.0	14.746394453030764	0.0
33	5.089585181842396E-4	0.0	0.0	15.120733443155272	0.0
34	5.089585181842396E-4	0.0	0.0	15.491594550072188	0.0
35	5.089585181842396E-4	0.0	0.0	15.88739462438013	0.0
36	5.089585181842396E-4	0.0	0.0	16.258679863395535	0.0
37	5.089585181842396E-4	0.0	0.0	16.64116218981099	0.0
38	5.089585181842396E-4	0.0	0.0	17.019063889562787	0.0
39	5.089585181842396E-4	0.0	0.0	17.41554257522831	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAACGCG	45	3.8562575E-10	45.0	1
CCGGTAT	20	7.033361E-4	45.0	42
TACCGGT	20	7.033361E-4	45.0	40
TAATCCG	20	7.033361E-4	45.0	1
CATACGA	85	0.0	42.35294	18
TACGCGG	115	0.0	41.08696	2
AGTTACG	50	1.0822987E-9	40.5	1
ATTAACG	40	3.4583172E-7	39.375	16
TCTAGCG	40	3.4583172E-7	39.375	1
TTACGCG	40	3.4583172E-7	39.375	1
CACGACC	70	0.0	38.57143	27
CGTTTTT	2080	0.0	38.07692	1
GCGTAAG	95	0.0	37.894737	1
ACCTCGC	30	1.1399397E-4	37.499996	17
ACGATTG	60	1.5643309E-10	37.499996	1
AATGCGA	60	1.5643309E-10	37.499996	25
TATAGCG	60	1.5643309E-10	37.499996	1
GTACGAC	30	1.1399397E-4	37.499996	38
CGGTCTA	55	2.750312E-9	36.818184	31
TCCGAAT	25	0.0021071308	36.0	22
>>END_MODULE