FastQCFastQC Report
Sat 18 Jun 2016
SRR3550252_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550252_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences368310
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTCTGC3960.10751812332002932No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3820.1037169775460889No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGG406.7939254E-945.0000042
ATTCGAT207.027036E-445.00000412
CTAAGCG207.027036E-445.0000041
TCGTGCG207.027036E-445.0000041
AACGAGC406.7939254E-945.00000415
TTAGTGC302.1614705E-645.00000416
CACAACG406.7939254E-945.00000412
TAGCACG253.8856266E-545.01
CGTAAGG650.041.5384642
TACACGC650.041.53846435
CGTCATA501.0768417E-940.538
TACGGGA1450.040.344834
CAACGAG451.9221261E-840.014
TCGATGG451.9221261E-840.01
TTCGTGG403.4496952E-739.3750042
CTACGGG1000.038.253
CGGGAAT659.094947E-1238.0769276
ACGTTGG301.1384161E-437.5000042
CGACCGG301.1384161E-437.50000433
ATGCTAC301.1384161E-437.50000411