##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550252_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	368310
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.62017593874725	34.0	31.0	34.0	31.0	34.0
2	32.80680676603948	34.0	31.0	34.0	31.0	34.0
3	32.8305747875431	34.0	31.0	34.0	31.0	34.0
4	36.220121636664764	37.0	37.0	37.0	35.0	37.0
5	36.22483777252857	37.0	37.0	37.0	35.0	37.0
6	36.25114170128425	37.0	37.0	37.0	35.0	37.0
7	36.26850479215878	37.0	37.0	37.0	35.0	37.0
8	36.2059243571991	37.0	37.0	37.0	35.0	37.0
9	37.76155412560072	39.0	38.0	39.0	35.0	39.0
10	37.66379137139909	39.0	37.0	39.0	35.0	39.0
11	37.60441747441014	39.0	37.0	39.0	35.0	39.0
12	37.420618500719506	39.0	37.0	39.0	35.0	39.0
13	37.38366593358855	39.0	37.0	39.0	35.0	39.0
14	38.693586924058536	40.0	38.0	41.0	35.0	41.0
15	38.76380494691971	40.0	38.0	41.0	35.0	41.0
16	38.82379788764899	40.0	38.0	41.0	35.0	41.0
17	38.819103472618174	40.0	38.0	41.0	35.0	41.0
18	38.760413781868536	40.0	38.0	41.0	35.0	41.0
19	38.71422986071516	40.0	38.0	41.0	35.0	41.0
20	38.63954006136135	40.0	38.0	41.0	35.0	41.0
21	38.562699899541144	40.0	38.0	41.0	35.0	41.0
22	38.511411582634196	40.0	38.0	41.0	35.0	41.0
23	38.423789742336616	40.0	37.0	41.0	34.0	41.0
24	38.387673427275935	40.0	37.0	41.0	34.0	41.0
25	38.37027775515191	40.0	37.0	41.0	34.0	41.0
26	38.32181586163829	40.0	37.0	41.0	34.0	41.0
27	38.2799978279167	40.0	37.0	41.0	34.0	41.0
28	38.234671879666585	40.0	37.0	41.0	34.0	41.0
29	38.18979392359697	40.0	37.0	41.0	34.0	41.0
30	38.12120767831446	40.0	37.0	41.0	34.0	41.0
31	38.01511226955554	40.0	36.0	41.0	34.0	41.0
32	37.91647796693003	40.0	36.0	41.0	34.0	41.0
33	37.840188971247045	40.0	36.0	41.0	34.0	41.0
34	37.750886481496565	40.0	36.0	41.0	33.0	41.0
35	37.70076294425891	40.0	36.0	41.0	33.0	41.0
36	37.605270017105155	40.0	36.0	41.0	33.0	41.0
37	37.543802774836415	40.0	35.0	41.0	33.0	41.0
38	37.46454345524151	40.0	35.0	41.0	33.0	41.0
39	37.388178436643045	40.0	35.0	41.0	33.0	41.0
40	37.28214547527898	40.0	35.0	41.0	33.0	41.0
41	37.208685618093455	40.0	35.0	41.0	33.0	41.0
42	37.11674947734246	39.0	35.0	41.0	32.0	41.0
43	37.07230593793272	39.0	35.0	41.0	32.0	41.0
44	36.95768510222367	39.0	35.0	41.0	32.0	41.0
45	36.86116586571095	39.0	35.0	41.0	32.0	41.0
46	36.81171567429611	39.0	35.0	41.0	32.0	41.0
47	36.72058048926176	39.0	35.0	41.0	31.0	41.0
48	36.65708234910809	39.0	35.0	41.0	31.0	41.0
49	36.53468002497896	39.0	35.0	41.0	31.0	41.0
50	36.432046373978444	39.0	35.0	41.0	31.0	41.0
51	35.505723439493906	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	3.0
10	17.0
11	9.0
12	5.0
13	14.0
14	16.0
15	14.0
16	19.0
17	40.0
18	87.0
19	162.0
20	221.0
21	314.0
22	508.0
23	813.0
24	1229.0
25	1664.0
26	2179.0
27	2575.0
28	2842.0
29	3506.0
30	4061.0
31	5178.0
32	6692.0
33	9507.0
34	20199.0
35	30415.0
36	23244.0
37	35836.0
38	63552.0
39	153156.0
40	230.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.69767315576552	19.99185468762727	25.024300181911975	10.286171974695229
2	33.34256468735576	23.24373489723331	25.268931063506283	18.144769351904646
3	32.88479813200836	23.961065406858353	25.31210121908175	17.842035242051534
4	28.71168309304662	25.778284597214302	26.309901984741114	19.200130324997964
5	25.876299856099482	31.111020607640306	23.844044419103472	19.168635117156743
6	26.971029838994326	36.22356167359018	22.476717982134613	14.328690505280878
7	83.41370041541093	6.20265537183351	6.306915370204447	4.076728842551112
8	85.05090820232955	5.404686269718444	6.068257717683473	3.476147810268524
9	79.56884146507018	6.9346474437294665	7.845836387825473	5.650674703374874
10	42.281773506013955	26.301756672368388	16.441856045179332	14.974613776438325
11	33.90106160571258	26.23985229833564	21.343162010263093	18.515924085688688
12	29.783877711710243	23.944231761288044	26.555890418397542	19.716000108604163
13	26.731557655236077	24.971084141076812	27.77714425348212	20.52021395020499
14	22.65428579186012	28.306046536884686	27.41196274877142	21.62770492248378
15	22.585050636691918	26.09893839428742	31.213651543536695	20.102359425483968
16	25.415003665390568	24.827726643316772	30.28481442263311	19.47245526865955
17	25.196166272976566	24.15845347669083	27.321278271021693	23.324101979310907
18	25.80217751350764	25.196166272976566	27.634058266134502	21.36759794738128
19	26.174689799353807	26.84043333061823	25.92082756373707	21.064049306290897
20	28.731774863566017	25.796475794846735	25.671581005131543	19.800168336455705
21	26.889576715267033	25.58361163150607	26.599332084385434	20.927479568841463
22	25.971871521272842	25.61021965192365	25.98300344818224	22.43490537862127
23	25.23825038690234	26.29442589123293	26.608291927995438	21.859031793869292
24	24.38597920230241	25.200238929162932	27.78528956585485	22.62849230267981
25	24.069398061415654	26.3253780782493	26.2952404224702	23.309983437864844
26	23.74575764980587	27.592788683446013	26.12961907089137	22.53183459585675
27	23.218484428877847	27.676956911297545	27.062257337568894	22.04230132225571
28	22.284217099725776	28.13445195623252	27.319106187722298	22.262224756319405
29	23.904319730661673	27.25584426162743	26.780429529472453	22.05940647823844
30	24.042247020173225	26.320490890825667	26.468464065596915	23.168798023404197
31	24.85949336157042	27.7657408161603	24.907279194157095	22.467486628112187
32	24.833156851565256	27.77660123265727	25.451929081480273	21.938312834297193
33	25.287393771551137	27.586272433547826	25.182590752355356	21.943743042545684
34	23.673807390513428	27.927561021965193	26.597431511498463	21.801200076022916
35	23.662403953191603	27.131764003149524	25.41799027992723	23.78784176373164
36	24.48535201324971	29.14718579457522	24.798403518774943	21.569058673400125
37	23.971111292118053	29.319051885639812	25.211099345659903	21.498737476582228
38	23.179929950313593	29.052428660639134	24.429963889115147	23.337677499932123
39	25.43482392549754	26.377236567022344	25.507860226439682	22.680079281040427
40	24.50381472129456	26.451630420026607	26.73780239472184	22.30675246395699
41	22.157150226711195	27.92294534495398	26.02020037468437	23.89970405365046
42	23.98794493768836	27.067959056229807	25.489126007982403	23.454969998099426
43	23.937987021802286	27.02804702560343	25.38622356167359	23.647742390920694
44	23.07078276451902	27.493687382911137	26.21460182998018	23.220928022589664
45	23.488365778827617	26.521951616844508	25.48939751839483	24.500285085933047
46	23.43759333170427	27.713067796149982	25.532024653145445	23.3173142190003
47	23.865222231272572	27.55016154869539	26.345198338356273	22.239417881675763
48	23.612989058130378	26.207542559257146	27.117645461703454	23.061822920909016
49	23.190247345985718	26.582769949227554	27.147783117482554	23.07919958730417
50	21.72409111889441	27.148869159132254	26.874643642583695	24.252396079389644
51	22.226928402704242	26.278678287312317	26.429638076620236	25.0647552333632
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	117.0
1	302.5
2	488.0
3	769.5
4	1051.0
5	795.0
6	539.0
7	546.0
8	553.0
9	600.0
10	647.0
11	635.5
12	624.0
13	644.0
14	664.0
15	663.0
16	662.0
17	715.5
18	769.0
19	754.0
20	739.0
21	973.5
22	1208.0
23	1376.0
24	1544.0
25	1722.0
26	2497.5
27	3095.0
28	3869.5
29	4644.0
30	5038.0
31	5432.0
32	6433.5
33	7435.0
34	8417.0
35	9399.0
36	10672.0
37	11945.0
38	12610.5
39	13276.0
40	15169.0
41	17062.0
42	18947.0
43	20832.0
44	22088.5
45	23345.0
46	24673.5
47	26002.0
48	27675.0
49	29348.0
50	28980.5
51	28613.0
52	26401.5
53	24190.0
54	22893.5
55	21597.0
56	21173.0
57	20749.0
58	20249.5
59	19750.0
60	19123.0
61	18496.0
62	16922.5
63	15349.0
64	12978.5
65	10608.0
66	9462.0
67	8316.0
68	7088.5
69	5861.0
70	4925.0
71	3989.0
72	3330.5
73	2672.0
74	2301.0
75	1728.5
76	1527.0
77	1085.5
78	644.0
79	532.5
80	421.0
81	284.5
82	148.0
83	114.0
84	80.0
85	50.0
86	20.0
87	22.5
88	25.0
89	14.0
90	3.0
91	2.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	368310.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.183294234694355
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.77899999999455	18.968230884025893
2	7.623999999999405	4.1449087049058715
3	3.2279999999997475	2.6324302136875954
4	2.096999999999836	2.2801347204059845
5	1.7390000000076529	2.3635874337170755
6	1.5029999999999186	2.4513894740846043
7	1.266999999999901	2.4108863656748545
8	1.0719999999999164	2.3312393135672056
9	0.9839999999999232	2.407352537424344
>10	10.600999999999171	57.608468139813866
>50	0.08699999999999321	1.4578400698065443
>100	0.018999999999998515	0.9435321428861674
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTCTGC	396	0.10751812332002932	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	382	0.1037169775460889	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07520838424153567	0.0
2	0.0	0.0	0.0	0.32771306779615	0.0
3	0.0	0.0	0.0	0.4450055659634547	0.0
4	0.0	0.0	0.0	0.5883630637234938	0.0
5	0.0	0.0	0.0	1.0230512340148246	0.0
6	0.0	0.0	0.0	1.2891314381906547	0.0
7	0.0	0.0	0.0	1.5212728408134453	0.0
8	0.0	0.0	0.0	1.921750699139312	0.0
9	0.0	0.0	0.0	2.0732535092720807	0.0
10	0.0	0.0	0.0	2.447394857592789	0.0
11	0.0	0.0	0.0	2.9714099535717193	0.0
12	0.0	0.0	0.0	3.37840406179577	0.0
13	0.0	0.0	0.0	3.5570579131709703	0.0
14	0.0	0.0	0.0	3.6243924954522004	0.0
15	0.0	0.0	0.0	3.770736607748907	0.0
16	0.0	0.0	0.0	4.08351660286172	0.0
17	0.0	0.0	0.0	4.446526024273031	0.0
18	0.0	0.0	0.0	4.8516195596101115	0.0
19	0.0	0.0	0.0	5.068827889549564	2.7151041242431647E-4
20	0.0	0.0	0.0	5.302869865059325	2.7151041242431647E-4
21	0.0	0.0	0.0	5.594472048003041	2.7151041242431647E-4
22	0.0	0.0	0.0	5.88688876218403	2.7151041242431647E-4
23	0.0	0.0	0.0	6.201569330183813	2.7151041242431647E-4
24	0.0	0.0	0.0	6.471179169721159	2.7151041242431647E-4
25	2.7151041242431647E-4	0.0	0.0	6.719611197089408	2.7151041242431647E-4
26	2.7151041242431647E-4	0.0	0.0	6.938991610328256	2.7151041242431647E-4
27	2.7151041242431647E-4	0.0	0.0	7.153213325731042	2.7151041242431647E-4
28	2.7151041242431647E-4	0.0	0.0	7.373136759794738	2.7151041242431647E-4
29	2.7151041242431647E-4	0.0	0.0	7.613151964377834	2.7151041242431647E-4
30	5.430208248486329E-4	0.0	0.0	7.890364095463061	2.7151041242431647E-4
31	8.145312372729494E-4	0.0	0.0	8.139067633243736	2.7151041242431647E-4
32	8.145312372729494E-4	0.0	0.0	8.39537346257229	2.7151041242431647E-4
33	8.145312372729494E-4	0.0	0.0	8.654665906437511	2.7151041242431647E-4
34	8.145312372729494E-4	0.0	0.0	8.89929678803182	2.7151041242431647E-4
35	8.145312372729494E-4	0.0	0.0	9.156417148597649	2.7151041242431647E-4
36	8.145312372729494E-4	0.0	0.0	9.407564280090142	2.7151041242431647E-4
37	8.145312372729494E-4	0.0	0.0	9.657082349108089	2.7151041242431647E-4
38	8.145312372729494E-4	0.0	0.0	9.918003855447857	2.7151041242431647E-4
39	8.145312372729494E-4	0.0	0.0	10.200917705193994	2.7151041242431647E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGCGG	40	6.7939254E-9	45.000004	2
ATTCGAT	20	7.027036E-4	45.000004	12
CTAAGCG	20	7.027036E-4	45.000004	1
TCGTGCG	20	7.027036E-4	45.000004	1
AACGAGC	40	6.7939254E-9	45.000004	15
TTAGTGC	30	2.1614705E-6	45.000004	16
CACAACG	40	6.7939254E-9	45.000004	12
TAGCACG	25	3.8856266E-5	45.0	1
CGTAAGG	65	0.0	41.538464	2
TACACGC	65	0.0	41.538464	35
CGTCATA	50	1.0768417E-9	40.5	38
TACGGGA	145	0.0	40.34483	4
CAACGAG	45	1.9221261E-8	40.0	14
TCGATGG	45	1.9221261E-8	40.0	1
TTCGTGG	40	3.4496952E-7	39.375004	2
CTACGGG	100	0.0	38.25	3
CGGGAAT	65	9.094947E-12	38.076927	6
ACGTTGG	30	1.1384161E-4	37.500004	2
CGACCGG	30	1.1384161E-4	37.500004	33
ATGCTAC	30	1.1384161E-4	37.500004	11
>>END_MODULE