FastQCFastQC Report
Sat 18 Jun 2016
SRR3550251_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550251_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1365339
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126740.9282676317017239No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA19160.14033144881967044No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCAT207.0337526E-445.010
CGTTTTT56050.042.8724331
TATAGCG501.0822987E-940.51
CGGTCTA850.039.70588331
CATTAGA6500.038.7692317
ACCGGTA356.2491526E-638.57142641
CTTGACC8250.037.9090927
ACAACGA3000.037.50000413
CAACGAC1450.037.2413812
CGCACTA552.750312E-936.8181823
CTCAACT8500.036.7941172
TTGACCC7600.036.7105268
ACTTGAC8550.036.5789456
ATACGGA1050.036.4285744
GCTCAAC8750.036.2571451
GCATTAG7150.036.18881216
TCAACTT8700.035.9482773
ATTAGAA6800.035.73529418
TGCATTA6950.035.6115115
CGTAAGG1650.035.4545442
Produced by FastQC (version 0.11.5)