##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550250_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	608415
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.4150505822506	33.0	31.0	34.0	31.0	34.0
2	32.604026856668554	34.0	31.0	34.0	31.0	34.0
3	32.614120296179415	34.0	31.0	34.0	31.0	34.0
4	36.06157639111462	37.0	35.0	37.0	35.0	37.0
5	36.08863029346745	37.0	35.0	37.0	35.0	37.0
6	36.130862980038295	37.0	36.0	37.0	35.0	37.0
7	36.18411281773132	37.0	36.0	37.0	35.0	37.0
8	36.13609625009245	37.0	37.0	37.0	35.0	37.0
9	37.68427307019058	39.0	38.0	39.0	35.0	39.0
10	37.563934156784434	39.0	37.0	39.0	35.0	39.0
11	37.48407583639457	39.0	37.0	39.0	35.0	39.0
12	37.31730151294758	39.0	37.0	39.0	35.0	39.0
13	37.28653304076987	39.0	37.0	39.0	34.0	39.0
14	38.548727431111985	40.0	38.0	41.0	35.0	41.0
15	38.618010732805736	40.0	38.0	41.0	35.0	41.0
16	38.6858788820131	40.0	38.0	41.0	35.0	41.0
17	38.6846872611622	40.0	38.0	41.0	35.0	41.0
18	38.618101131628904	40.0	38.0	41.0	35.0	41.0
19	38.537342110237255	40.0	37.0	41.0	35.0	41.0
20	38.44075014587083	40.0	37.0	41.0	34.0	41.0
21	38.395282825045406	40.0	37.0	41.0	34.0	41.0
22	38.34037786708086	40.0	37.0	41.0	34.0	41.0
23	38.232020906782374	40.0	37.0	41.0	34.0	41.0
24	38.17530139789453	40.0	36.0	41.0	34.0	41.0
25	38.16067815553529	40.0	36.0	41.0	34.0	41.0
26	38.1244101476788	40.0	36.0	41.0	34.0	41.0
27	38.07162380940641	40.0	36.0	41.0	34.0	41.0
28	38.005004807573776	40.0	36.0	41.0	34.0	41.0
29	37.95281345791935	40.0	36.0	41.0	34.0	41.0
30	37.86387745206808	40.0	36.0	41.0	34.0	41.0
31	37.73958564466688	40.0	36.0	41.0	33.0	41.0
32	37.617239877386325	40.0	35.0	41.0	33.0	41.0
33	37.495918082230055	40.0	35.0	41.0	33.0	41.0
34	37.38889738089955	40.0	35.0	41.0	33.0	41.0
35	37.2858640894784	40.0	35.0	41.0	33.0	41.0
36	37.18456974269208	40.0	35.0	41.0	33.0	41.0
37	37.12653041098592	40.0	35.0	41.0	33.0	41.0
38	37.02031672460409	39.0	35.0	41.0	32.0	41.0
39	36.91666214672551	39.0	35.0	41.0	32.0	41.0
40	36.82737440727135	39.0	35.0	41.0	32.0	41.0
41	36.7586696580459	39.0	35.0	41.0	32.0	41.0
42	36.663175628477276	39.0	35.0	41.0	31.0	41.0
43	36.594247347616346	39.0	35.0	41.0	31.0	41.0
44	36.47732715334106	39.0	35.0	41.0	31.0	41.0
45	36.3662746644971	39.0	35.0	41.0	31.0	41.0
46	36.28604159989481	39.0	35.0	41.0	31.0	41.0
47	36.20456596237765	38.0	35.0	41.0	31.0	41.0
48	36.14588068998956	38.0	35.0	41.0	31.0	41.0
49	36.061775268525594	38.0	35.0	41.0	30.0	41.0
50	35.95382756835384	38.0	35.0	40.0	30.0	41.0
51	35.06918304118077	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	8.0
9	15.0
10	21.0
11	14.0
12	19.0
13	15.0
14	20.0
15	21.0
16	48.0
17	85.0
18	164.0
19	278.0
20	448.0
21	724.0
22	1128.0
23	1706.0
24	2711.0
25	3696.0
26	4632.0
27	5411.0
28	5925.0
29	6581.0
30	7832.0
31	9556.0
32	12342.0
33	17308.0
34	34677.0
35	52566.0
36	41830.0
37	62808.0
38	108643.0
39	226876.0
40	306.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.804689233500156	21.159899082041044	25.590098863440254	11.445312821018549
2	31.694813572972397	24.904218337812186	25.861952778942005	17.539015310273413
3	31.597018482450302	24.411133847784818	26.742601678130878	17.249245991633998
4	27.892474708874698	26.865050993154345	26.76922824059236	18.4732460573786
5	24.78226210727875	30.876293319526965	25.333366205632668	19.008078367561616
6	26.169308777725732	35.47989447991913	23.343277203882216	15.007519538472916
7	80.81441121602853	6.532383323882547	8.082476599031912	4.570728861057009
8	82.31026519727489	5.574484521256051	7.654479261688158	4.460771019780906
9	76.68203446660586	7.378680670266183	9.794465948406925	6.1448189147210375
10	42.04235595769335	25.807713485038995	17.458642538398955	14.6912880188687
11	31.171157844563336	24.71388772466162	23.577163613651866	20.53779081712318
12	27.935866143997107	22.662327523154428	28.66201523631074	20.739791096537726
13	24.768455741557982	25.844530460294372	29.332281419754608	20.054732378393037
14	20.975649844267483	27.849247635248968	29.29480699851253	21.880295521971025
15	20.076756818947594	26.22699966305893	32.16077841604826	21.535465101945217
16	21.83279504943172	24.215379305243953	31.021095798098337	22.93072984722599
17	21.842656739232265	25.14352867697213	27.658752660601728	25.355061923193873
18	22.33245399932612	25.30624655868116	29.545458280943105	22.81584116104961
19	23.240715629956526	27.267407937016674	26.472720100589235	23.01915633243756
20	25.0846872611622	26.01711003180395	26.974680111437095	21.923522595596758
21	23.592449232842714	27.246698388435526	27.52216825686415	21.63868412185761
22	23.330950091631532	25.534215954570485	25.916849518831718	25.21798443496627
23	22.179269084424284	26.57561039750828	26.746710715547778	24.49840980251966
24	22.822744343909996	25.533722870080457	28.97430207999474	22.669230706014808
25	21.763927582324566	27.416483814501614	26.512166859791424	24.307421743382395
26	21.753079723543962	28.148549920695576	25.541612221920897	24.55675813383957
27	22.022632578092256	27.338083380587264	27.273160589400327	23.366123451920153
28	20.901851532260054	26.850422819950197	27.89987097622511	24.34785467156464
29	21.567186870803646	24.98360494070659	27.370462595432393	26.078745593057373
30	22.191267473681613	27.340055718547372	27.180460705275184	23.28821610249583
31	24.563825678196626	27.813745551966996	23.8288010650625	23.79362770477388
32	23.314020857473928	28.824568756523096	24.076986925042938	23.784423460960035
33	23.198967809800877	28.34364701724974	24.284739856841135	24.172645316108248
34	21.472679010215067	27.324441376363172	25.175743530320588	26.027136083101173
35	21.815537092280763	27.18539155017546	25.727176351667858	25.271895005875923
36	22.50256814838556	28.897545261047146	24.408832786831358	24.191053803735937
37	22.733331689718366	29.184849157236425	25.677210456678417	22.40460869636679
38	21.733849428432894	29.956197661135903	24.85014340540585	23.459809505025355
39	21.84758758413254	28.85415382592474	25.145172291938888	24.153086298003828
40	22.280022681886543	26.808674999794547	26.531561516399165	24.37974080191974
41	20.288454426666007	26.798977671490675	25.741804524872002	27.170763376971312
42	21.36173499995891	26.529753539935736	25.671129081301412	26.437382378803942
43	22.930236762735962	24.769113187544686	27.173393160918124	25.12725688880123
44	21.73204145196946	25.99081219233582	26.071678048700313	26.205468306994405
45	21.625370840626875	26.268747483214582	25.645653049316667	26.460228626841875
46	22.01589375672855	27.420264128925158	25.823656550216544	24.740185564129746
47	20.922561080841202	26.623768316034287	27.846617851302153	24.60705275182236
48	21.33165684606724	25.28997477051026	27.85549337212265	25.52287501129985
49	21.241586745888906	25.197274886385117	28.82900651693334	24.732131850792634
50	20.543050385016805	24.85326627384269	28.65050993154344	25.95317340959707
51	20.916315343967522	25.511698429525897	26.140545515807467	27.43144071069911
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	453.0
1	834.0
2	1215.0
3	2238.5
4	3262.0
5	2394.5
6	1527.0
7	1572.0
8	1617.0
9	1756.5
10	1896.0
11	2010.0
12	2124.0
13	2097.5
14	2071.0
15	2061.5
16	2052.0
17	2061.0
18	2070.0
19	2037.0
20	2004.0
21	2102.0
22	2200.0
23	2395.5
24	2591.0
25	3416.5
26	4399.5
27	4557.0
28	5970.5
29	7384.0
30	8504.0
31	9624.0
32	10264.5
33	10905.0
34	12855.0
35	14805.0
36	15593.5
37	16382.0
38	18336.0
39	20290.0
40	23416.5
41	26543.0
42	29328.5
43	32114.0
44	36126.5
45	40139.0
46	44439.0
47	48739.0
48	51978.0
49	55217.0
50	53591.0
51	51965.0
52	45987.5
53	40010.0
54	36043.0
55	32076.0
56	29594.0
57	27112.0
58	26806.5
59	26501.0
60	25682.0
61	24863.0
62	23655.5
63	22448.0
64	20050.5
65	17653.0
66	16249.5
67	14846.0
68	12907.0
69	10968.0
70	9475.0
71	7982.0
72	6818.0
73	5654.0
74	4980.5
75	3518.5
76	2730.0
77	2118.0
78	1506.0
79	1146.0
80	786.0
81	627.5
82	469.0
83	385.0
84	301.0
85	228.5
86	156.0
87	94.0
88	32.0
89	25.5
90	19.0
91	13.0
92	7.0
93	4.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	608415.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.253433292839585
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.51150043739244	21.334591404500944
2	9.59577425796113	5.422271357809041
3	3.2862758432117696	2.7854572595416167
4	1.6709781032201283	1.8884347349250208
5	0.9946161852420189	1.405066102085698
6	0.6961716007878185	1.180154272993679
7	0.5196885066909617	1.0278089189793953
8	0.4357802241919719	0.9849829995637246
9	0.37269598619074745	0.9476947065914412
>10	5.896884796331028	42.45080982792507
>50	0.957076235219577	16.547184097609392
>100	0.059634560777273575	3.0985293519571644
>500	0.001753957669919811	0.38719228067958167
>1k	0.001169305113279874	0.5398226848382564
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1851	0.30423313034688493	No Hit
GCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGC	1417	0.23290024078959265	TruSeq Adapter, Index 19 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGCT	920	0.15121257694172563	TruSeq Adapter, Index 13 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGC	779	0.12803760591043942	TruSeq Adapter, Index 19 (95% over 23bp)
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	645	0.10601316535588372	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6436149667578873E-4	0.0	0.0	0.15828012129878455	0.0
2	1.6436149667578873E-4	0.0	0.0	0.6931124314818011	0.0
3	1.6436149667578873E-4	0.0	0.0	1.0239721242901638	0.0
4	1.6436149667578873E-4	0.0	0.0	1.4432583023101009	0.0
5	1.6436149667578873E-4	0.0	0.0	2.620250980005424	0.0
6	1.6436149667578873E-4	0.0	0.0	3.4128021169760774	0.0
7	1.6436149667578873E-4	0.0	0.0	4.136485786839575	0.0
8	1.6436149667578873E-4	0.0	0.0	5.298685929834077	0.0
9	1.6436149667578873E-4	0.0	0.0	5.764979495903289	0.0
10	1.6436149667578873E-4	0.0	0.0	6.759695273785163	0.0
11	1.6436149667578873E-4	0.0	0.0	8.08329840651529	0.0
12	1.6436149667578873E-4	0.0	0.0	9.057304635816013	0.0
13	1.6436149667578873E-4	0.0	0.0	9.48135729723955	0.0
14	1.6436149667578873E-4	0.0	0.0	9.666592703993162	0.0
15	3.2872299335157747E-4	0.0	0.0	9.950116285758899	0.0
16	3.2872299335157747E-4	0.0	0.0	10.50467197554301	0.0
17	3.2872299335157747E-4	0.0	0.0	11.237888612213704	0.0
18	4.930844900273662E-4	0.0	0.0	11.996581280869144	0.0
19	4.930844900273662E-4	0.0	0.0	12.480790250076018	0.0
20	4.930844900273662E-4	0.0	0.0	12.920292892187076	0.0
21	6.574459867031549E-4	0.0	0.0	13.488983670685306	0.0
22	6.574459867031549E-4	0.0	0.0	14.073124429871058	0.0
23	6.574459867031549E-4	0.0	0.0	14.680604521584774	0.0
24	6.574459867031549E-4	0.0	0.0	15.169908697188596	0.0
25	6.574459867031549E-4	0.0	0.0	15.59363263561878	0.0
26	6.574459867031549E-4	0.0	0.0	15.971006631986391	0.0
27	6.574459867031549E-4	0.0	0.0	16.340984361003592	0.0
28	6.574459867031549E-4	0.0	0.0	16.722467394788097	0.0
29	6.574459867031549E-4	0.0	0.0	17.13846634287452	0.0
30	6.574459867031549E-4	0.0	0.0	17.6137997912609	0.0
31	6.574459867031549E-4	0.0	0.0	18.02897693186394	0.0
32	6.574459867031549E-4	0.0	0.0	18.424595054362566	0.0
33	6.574459867031549E-4	0.0	0.0	18.793750975896387	0.0
34	6.574459867031549E-4	0.0	0.0	19.17342603321746	0.0
35	6.574459867031549E-4	0.0	0.0	19.606025492468135	0.0
36	6.574459867031549E-4	0.0	0.0	19.994740432106376	0.0
37	6.574459867031549E-4	0.0	0.0	20.387235686168157	0.0
38	6.574459867031549E-4	0.0	0.0	20.76428095954242	0.0
39	6.574459867031549E-4	0.0	0.0	21.127026782705883	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTATT	30	2.1638098E-6	45.000004	43
CTCACGA	50	2.1827873E-11	45.0	24
AGATCGG	20	7.0306665E-4	45.0	2
ACGTTAG	20	7.0306665E-4	45.0	1
TAGCGAA	20	7.0306665E-4	45.0	27
ACCGACA	20	7.0306665E-4	45.0	23
ACTTACG	25	3.888634E-5	45.0	34
CGTTCGC	20	7.0306665E-4	45.0	13
CGGATGT	20	7.0306665E-4	45.0	39
CGCTATA	20	7.0306665E-4	45.0	25
ACAGCGA	20	7.0306665E-4	45.0	21
GGACGTA	20	7.0306665E-4	45.0	8
AATTACG	20	7.0306665E-4	45.0	1
ACGTATG	20	7.0306665E-4	45.0	1
CGGTCTA	50	2.1827873E-11	45.0	31
CCAACGC	20	7.0306665E-4	45.0	32
TAGTGCG	25	3.888634E-5	45.0	1
TATGTCG	35	1.2106648E-7	45.0	1
GCGTAAG	70	0.0	45.0	1
GGCCGAT	60	3.6379788E-12	41.250004	8
>>END_MODULE