SRR3550249_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550249_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	817309
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC	2700	0.3303524126126104	TruSeq Adapter, Index 15 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC	2090	0.2557172379112429	TruSeq Adapter, Index 15 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1967	0.24066785022555728	No Hit
CTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGCT	1665	0.20371732111110977	TruSeq Adapter, Index 14 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1502	0.18377382360894104	No Hit
TCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC	1068	0.13067273210009922	TruSeq Adapter, Index 15 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTTACG	20	7.0320896E-4	45.0	1
GCCGATA	20	7.0320896E-4	45.0	9
CTAACGG	50	2.1827873E-11	45.0	2
TCGGACA	20	7.0320896E-4	45.0	35
CCGTAAG	25	3.8898135E-5	45.0	1
CGTACTG	20	7.0320896E-4	45.0	12
CGTGTTA	25	3.8898135E-5	45.0	44
TCGACTG	35	1.211356E-7	45.0	1
TATCGTC	20	7.0320896E-4	45.0	12
TGCGTCG	25	3.8898135E-5	45.0	1
GCATAAC	30	2.1647302E-6	44.999996	33
CGACGGT	180	0.0	41.25	28
GTGCAAG	115	0.0	41.08696	1
ACCGCTA	55	6.002665E-11	40.909092	8
CGTAAGG	105	0.0	40.714287	2
CGGTCTA	185	0.0	40.135136	31
CGTTCCG	45	1.9272193E-8	40.0	26
GCGACGT	85	0.0	39.705883	17
CGCGAGG	85	0.0	39.705883	2
TCACGAC	210	0.0	39.642857	25