##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3550249_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 817309 Sequences flagged as poor quality 0 Sequence length 51 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.41662945103994 33.0 31.0 34.0 31.0 34.0 2 32.59792073744447 34.0 31.0 34.0 31.0 34.0 3 32.621450393914664 34.0 31.0 34.0 31.0 34.0 4 36.06058418541824 37.0 35.0 37.0 35.0 37.0 5 36.10233950684503 37.0 35.0 37.0 35.0 37.0 6 36.13483761955393 37.0 36.0 37.0 35.0 37.0 7 36.215165867499316 37.0 37.0 37.0 35.0 37.0 8 36.16534872367733 37.0 37.0 37.0 35.0 37.0 9 37.700721514139694 39.0 38.0 39.0 35.0 39.0 10 37.591569406430125 39.0 37.0 39.0 35.0 39.0 11 37.532739759381094 39.0 37.0 39.0 35.0 39.0 12 37.38677171057703 39.0 37.0 39.0 35.0 39.0 13 37.34050401989945 39.0 37.0 39.0 35.0 39.0 14 38.61408720569577 40.0 38.0 41.0 35.0 41.0 15 38.683589682727096 40.0 38.0 41.0 35.0 41.0 16 38.745689818661 40.0 38.0 41.0 35.0 41.0 17 38.73430734275531 40.0 38.0 41.0 35.0 41.0 18 38.692816303258624 40.0 38.0 41.0 35.0 41.0 19 38.643848287489796 40.0 38.0 41.0 35.0 41.0 20 38.55965613984429 40.0 38.0 41.0 35.0 41.0 21 38.478815233895624 40.0 37.0 41.0 34.0 41.0 22 38.43094472225315 40.0 37.0 41.0 34.0 41.0 23 38.32879730921842 40.0 37.0 41.0 34.0 41.0 24 38.27732595627847 40.0 37.0 41.0 34.0 41.0 25 38.283794745928404 40.0 37.0 41.0 34.0 41.0 26 38.25134802137258 40.0 37.0 41.0 34.0 41.0 27 38.234846306598854 40.0 37.0 41.0 34.0 41.0 28 38.173311440348755 40.0 37.0 41.0 34.0 41.0 29 38.137243074528726 40.0 37.0 41.0 34.0 41.0 30 38.05024170784856 40.0 36.0 41.0 34.0 41.0 31 37.89786726929472 40.0 36.0 41.0 34.0 41.0 32 37.800292178356045 40.0 36.0 41.0 33.0 41.0 33 37.71328958814842 40.0 36.0 41.0 33.0 41.0 34 37.60643648852515 40.0 36.0 41.0 33.0 41.0 35 37.51602392730289 40.0 35.0 41.0 33.0 41.0 36 37.41389976128979 40.0 35.0 41.0 33.0 41.0 37 37.32368174093274 40.0 35.0 41.0 33.0 41.0 38 37.2178172514924 40.0 35.0 41.0 33.0 41.0 39 37.17233261838546 39.0 35.0 41.0 32.0 41.0 40 37.07254171922737 39.0 35.0 41.0 32.0 41.0 41 36.980117678870535 39.0 35.0 41.0 32.0 41.0 42 36.848402501379525 39.0 35.0 41.0 32.0 41.0 43 36.810610185376646 39.0 35.0 41.0 31.0 41.0 44 36.71977795423763 39.0 35.0 41.0 31.0 41.0 45 36.61130735132 39.0 35.0 41.0 31.0 41.0 46 36.51511484640448 39.0 35.0 41.0 31.0 41.0 47 36.41843415403476 39.0 35.0 41.0 31.0 41.0 48 36.382901693239646 39.0 35.0 41.0 31.0 41.0 49 36.260337277578 38.0 35.0 41.0 31.0 41.0 50 36.137426603646844 38.0 35.0 40.0 31.0 41.0 51 35.141093515426846 37.0 34.0 40.0 28.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 8.0 9 20.0 10 17.0 11 16.0 12 23.0 13 20.0 14 23.0 15 39.0 16 57.0 17 87.0 18 178.0 19 312.0 20 498.0 21 813.0 22 1211.0 23 1893.0 24 2871.0 25 3991.0 26 5130.0 27 6115.0 28 7083.0 29 8297.0 30 10207.0 31 12901.0 32 16542.0 33 23183.0 34 45532.0 35 69062.0 36 55773.0 37 83387.0 38 150107.0 39 311321.0 40 592.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.23750258470175 21.512793814824015 24.540412500045882 10.709291100428358 2 32.65460187028407 24.396892729677518 25.376326456701197 17.572178943337217 3 32.11588273223469 24.859508460080583 26.187769864274102 16.83683894341063 4 28.667737661031506 26.382433082224715 25.592156699608104 19.357672557135675 5 24.592534769591428 31.243262951955746 24.152187238853358 20.012015039599465 6 25.964843162133295 36.144224522181936 23.107294793034214 14.783637522650553 7 81.54529070400545 6.170983067603562 7.518820911063012 4.764905317327963 8 81.67327167570649 5.619661596776739 7.572411413553503 5.134655313963262 9 75.7977704882731 7.455931599921205 9.956577010653254 6.789720901152441 10 40.372123639896294 26.78264891246762 17.56814130273862 15.277086144897462 11 30.00909080898412 25.104825714631794 24.38429039690007 20.501793079484017 12 28.329921730948758 23.233929884535716 29.062814675967108 19.37333370854842 13 24.78059093928979 26.129897015694187 29.129863980452924 19.959648064563098 14 20.72055978828081 28.124858529638118 28.12840675925507 23.026174922826005 15 19.03710836415603 26.28858852649365 32.958893148123906 21.715409961226413 16 21.395824590210065 24.628995887724226 30.06525071912826 23.909928802937443 17 21.420295139292485 25.58065554153937 27.009001491479967 25.990047827688183 18 22.010402430414935 26.155224033994486 29.815284060251386 22.019089475339193 19 22.614213229023537 28.428415691005483 25.95065024366549 23.006720836305487 20 25.028477601494657 26.04473950488738 27.60339112869184 21.323391764926118 21 23.71648911244095 27.808699035493305 26.723797241924412 21.751014610141333 22 23.099341864582428 25.362622949215048 25.77519640674457 25.76283877945795 23 21.527476144273464 26.645002073879038 27.20476588413929 24.62275589770821 24 22.757488293901083 24.964976526625794 29.81797582065045 22.459559358822673 25 22.0637482274146 27.955522329987804 25.762961132203365 24.217768310394234 26 21.153076743312504 27.554694736017833 25.760636430040535 25.531592090629125 27 21.698892340595783 26.83195706886869 27.234497601274427 24.2346529892611 28 20.075026703486685 26.325906113844333 28.876960855686164 24.722106326982818 29 22.065705871341194 24.355659854473643 28.262994779208356 25.31563949497681 30 24.178370726371543 27.55445003052701 27.01169325187903 21.255485991222415 31 24.48498670637421 29.423632922187327 24.570633628162668 21.520746743275797 32 24.62214413398115 26.989425052214035 25.312825381832333 23.075605431972484 33 24.29166936862313 28.143700852431575 24.380619814537706 23.184009964407586 34 22.958758560103952 26.3802307328073 27.095260176995485 23.56575053009327 35 24.32727401753804 25.27636426369953 27.364680922392875 23.03168079636955 36 25.5718461438697 28.67495647301082 25.01257174459109 20.74062563852839 37 23.635981005959803 28.44811448301683 27.96531054962077 19.950593961402603 38 21.60969718919038 28.022938692709857 28.81884330161542 21.54852081648434 39 23.756987871172345 26.971072140402224 27.41668083919301 21.85525914923242 40 25.398472303620785 25.020402320297464 28.580133095316455 21.000992280765292 41 23.393600217298477 24.762238027477977 27.872811874089237 23.97134988113431 42 23.351755578367545 25.089653974200697 27.33678449643892 24.22180595099283 43 23.72162792774826 25.111922173865697 27.884435384903384 23.28201451348266 44 22.360208929548065 25.28174778449766 27.452285488107925 24.90575779784635 45 22.833346996056573 26.19119574114564 26.428927125481305 24.546530137316484 46 23.8760370924583 26.485943504843334 27.477367800917403 22.160651601780966 47 22.137404580152673 25.92764792752802 30.041147228282085 21.893800264037225 48 21.972473079337192 24.490859638153992 29.878173371393196 23.658493911115624 49 23.265986303833678 24.149740183945116 29.843058133459927 22.741215378761275 50 21.818553325608796 23.171407631630142 30.535207614256056 24.47483142850501 51 21.001114633510703 23.635124536741916 28.59187895887602 26.77188187087136 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 558.0 1 1051.0 2 1544.0 3 2262.5 4 2981.0 5 2180.0 6 1379.0 7 1436.0 8 1493.0 9 1570.0 10 1647.0 11 1780.5 12 1914.0 13 1865.5 14 1817.0 15 1671.5 16 1526.0 17 1608.0 18 1690.0 19 1723.0 20 1756.0 21 1838.0 22 1920.0 23 2279.5 24 2639.0 25 3030.5 26 4570.0 27 5718.0 28 7139.5 29 8561.0 30 10709.5 31 12858.0 32 15461.0 33 18064.0 34 21652.0 35 25240.0 36 26874.5 37 28509.0 38 29536.0 39 30563.0 40 34009.0 41 37455.0 42 41751.5 43 46048.0 44 49998.0 45 53948.0 46 60708.5 47 67469.0 48 74792.0 49 82115.0 50 79703.0 51 77291.0 52 65443.5 53 53596.0 54 48309.5 55 43023.0 56 40595.5 57 38168.0 58 36356.5 59 34545.0 60 32246.0 61 29947.0 62 26697.5 63 23448.0 64 22201.5 65 20955.0 66 18177.5 67 15400.0 68 13062.5 69 10725.0 70 9917.5 71 9110.0 72 7795.0 73 6480.0 74 5190.0 75 3521.0 76 3142.0 77 2591.0 78 2040.0 79 1750.5 80 1461.0 81 1036.0 82 611.0 83 431.5 84 252.0 85 198.0 86 144.0 87 161.0 88 178.0 89 109.5 90 41.0 91 26.5 92 12.0 93 8.0 94 4.0 95 2.5 96 1.0 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 817309.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.20696561691256 #Duplication Level Percentage of deduplicated Percentage of total 1 71.14377458634524 18.644624544417276 2 11.571746508645287 6.0652072575939 3 4.437700421952606 3.4889598712881082 4 2.2256134952092155 2.33306305381938 5 1.3426876409635295 1.759388442049233 6 0.8942530996242162 1.406139614080159 7 0.6547121259998523 1.2010612722537697 8 0.5054290632972999 1.0596609666896528 9 0.42395193459741104 0.9999444395896123 >10 5.312335338421443 34.10248331051088 >50 1.4057137350211661 23.86970417056406 >100 0.07692260678476433 3.3278463593749286 >500 0.0023452014263647663 0.39076657837893464 >1k 0.0028142417116377196 1.3511501193901383 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC 2700 0.3303524126126104 TruSeq Adapter, Index 15 (95% over 22bp) CCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC 2090 0.2557172379112429 TruSeq Adapter, Index 15 (95% over 22bp) GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT 1967 0.24066785022555728 No Hit CTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGCT 1665 0.20371732111110977 TruSeq Adapter, Index 14 (95% over 22bp) CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1502 0.18377382360894104 No Hit TCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC 1068 0.13067273210009922 TruSeq Adapter, Index 15 (95% over 22bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.670582362362338E-4 0.0 0.0 0.21534083192525716 0.0 2 3.670582362362338E-4 0.0 0.0 1.0681394674474403 0.0 3 3.670582362362338E-4 0.0 0.0 1.4657858900366936 0.0 4 3.670582362362338E-4 0.0 0.0 1.9413710114534406 0.0 5 3.670582362362338E-4 0.0 0.0 3.2998535437637417 0.0 6 3.670582362362338E-4 0.0 0.0 4.064802908080053 0.0 7 3.670582362362338E-4 0.0 0.0 4.815559353928563 0.0 8 3.670582362362338E-4 0.0 0.0 5.943039902900861 0.0 9 3.670582362362338E-4 0.0 0.0 6.381185084221512 0.0 10 3.670582362362338E-4 0.0 0.0 7.4109057896095605 0.0 11 3.670582362362338E-4 0.0 0.0 8.922451606430371 0.0 12 3.670582362362338E-4 0.0 0.0 10.01824279434094 0.0 13 3.670582362362338E-4 0.0 0.0 10.436811536395659 0.0 14 3.670582362362338E-4 0.0 0.0 10.609818318408337 0.0 15 3.670582362362338E-4 0.0 0.0 10.955709529688281 0.0 16 3.670582362362338E-4 0.0 0.0 11.682729542926849 0.0 17 3.670582362362338E-4 0.0 0.0 12.569909299909826 0.0 18 3.670582362362338E-4 0.0 0.0 13.51227014507365 0.0 19 3.670582362362338E-4 0.0 0.0 14.010368171646219 0.0 20 3.670582362362338E-4 0.0 0.0 14.558997882073976 0.0 21 3.670582362362338E-4 0.0 0.0 15.239523852055955 0.0 22 3.670582362362338E-4 0.0 0.0 15.959080347824385 0.0 23 3.670582362362338E-4 0.0 0.0 16.6785144908474 0.0 24 3.670582362362338E-4 0.0 0.0 17.278287648857408 0.0 25 3.670582362362338E-4 0.0 0.0 17.79192447409731 0.0 26 3.670582362362338E-4 0.0 0.0 18.236554350924802 0.0 27 3.670582362362338E-4 0.0 0.0 18.673353652045922 0.0 28 3.670582362362338E-4 0.0 0.0 19.144044663646184 0.0 29 3.670582362362338E-4 0.0 0.0 19.643855628654524 0.0 30 3.670582362362338E-4 0.0 0.0 20.23310645055909 0.0 31 4.894109816483117E-4 0.0 0.0 20.69278571507227 0.0 32 4.894109816483117E-4 0.0 0.0 21.14634734231484 0.0 33 4.894109816483117E-4 0.0 0.0 21.621687758240764 0.0 34 4.894109816483117E-4 0.0 0.0 22.102411694964818 0.0 35 4.894109816483117E-4 0.0 0.0 22.678081362128644 0.0 36 6.117637270603896E-4 0.0 0.0 23.143388852930777 0.0 37 6.117637270603896E-4 0.0 0.0 23.599642240572415 0.0 38 7.341164724724676E-4 0.0 0.0 24.052469751342514 0.0 39 7.341164724724676E-4 0.0 0.0 24.53013486943127 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTTACG 20 7.0320896E-4 45.0 1 GCCGATA 20 7.0320896E-4 45.0 9 CTAACGG 50 2.1827873E-11 45.0 2 TCGGACA 20 7.0320896E-4 45.0 35 CCGTAAG 25 3.8898135E-5 45.0 1 CGTACTG 20 7.0320896E-4 45.0 12 CGTGTTA 25 3.8898135E-5 45.0 44 TCGACTG 35 1.211356E-7 45.0 1 TATCGTC 20 7.0320896E-4 45.0 12 TGCGTCG 25 3.8898135E-5 45.0 1 GCATAAC 30 2.1647302E-6 44.999996 33 CGACGGT 180 0.0 41.25 28 GTGCAAG 115 0.0 41.08696 1 ACCGCTA 55 6.002665E-11 40.909092 8 CGTAAGG 105 0.0 40.714287 2 CGGTCTA 185 0.0 40.135136 31 CGTTCCG 45 1.9272193E-8 40.0 26 GCGACGT 85 0.0 39.705883 17 CGCGAGG 85 0.0 39.705883 2 TCACGAC 210 0.0 39.642857 25 >>END_MODULE