##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550248_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1050624
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.45234641508284	33.0	31.0	34.0	31.0	34.0
2	32.638875563474656	34.0	31.0	34.0	31.0	34.0
3	32.65362489339669	34.0	31.0	34.0	31.0	34.0
4	36.085679558053116	37.0	35.0	37.0	35.0	37.0
5	36.11810219450536	37.0	35.0	37.0	35.0	37.0
6	36.156113890411795	37.0	36.0	37.0	35.0	37.0
7	36.23024983248051	37.0	37.0	37.0	35.0	37.0
8	36.19483754416423	37.0	37.0	37.0	35.0	37.0
9	37.743453414351855	39.0	38.0	39.0	35.0	39.0
10	37.60949588054337	39.0	37.0	39.0	35.0	39.0
11	37.54380825109649	39.0	37.0	39.0	35.0	39.0
12	37.38341119182505	39.0	37.0	39.0	35.0	39.0
13	37.34087266234162	39.0	37.0	39.0	35.0	39.0
14	38.60883246527778	40.0	38.0	41.0	35.0	41.0
15	38.67378053423489	40.0	38.0	41.0	35.0	41.0
16	38.740173458820664	40.0	38.0	41.0	35.0	41.0
17	38.736120629264136	40.0	38.0	41.0	35.0	41.0
18	38.696197688230995	40.0	38.0	41.0	35.0	41.0
19	38.64136836775098	40.0	38.0	41.0	35.0	41.0
20	38.5634756106847	40.0	38.0	41.0	35.0	41.0
21	38.52431221826267	40.0	38.0	41.0	35.0	41.0
22	38.461495263767056	40.0	37.0	41.0	34.0	41.0
23	38.38688055860136	40.0	37.0	41.0	34.0	41.0
24	38.32338591161062	40.0	37.0	41.0	34.0	41.0
25	38.33144588358918	40.0	37.0	41.0	34.0	41.0
26	38.2801839668616	40.0	37.0	41.0	34.0	41.0
27	38.24355906585039	40.0	37.0	41.0	34.0	41.0
28	38.18048226577729	40.0	37.0	41.0	34.0	41.0
29	38.15103405214425	40.0	37.0	41.0	34.0	41.0
30	38.08790775767544	40.0	36.0	41.0	34.0	41.0
31	37.96591834947612	40.0	36.0	41.0	34.0	41.0
32	37.881659851669106	40.0	36.0	41.0	34.0	41.0
33	37.81595889680799	40.0	36.0	41.0	33.0	41.0
34	37.7370410346613	40.0	36.0	41.0	33.0	41.0
35	37.674940797088205	40.0	36.0	41.0	33.0	41.0
36	37.57811643366228	40.0	35.0	41.0	33.0	41.0
37	37.53134518153021	40.0	35.0	41.0	33.0	41.0
38	37.4626250685307	40.0	35.0	41.0	33.0	41.0
39	37.376619037828945	40.0	35.0	41.0	33.0	41.0
40	37.29884716130604	40.0	35.0	41.0	33.0	41.0
41	37.236670778508774	39.0	35.0	41.0	33.0	41.0
42	37.15897123994883	39.0	35.0	41.0	33.0	41.0
43	37.11491170961257	39.0	35.0	41.0	33.0	41.0
44	37.029298778630604	39.0	35.0	41.0	32.0	41.0
45	36.91715209247076	39.0	35.0	41.0	32.0	41.0
46	36.83154867964181	39.0	35.0	41.0	32.0	41.0
47	36.755651879264136	39.0	35.0	41.0	32.0	41.0
48	36.715720371893276	39.0	35.0	41.0	32.0	41.0
49	36.63341690271686	39.0	35.0	41.0	32.0	41.0
50	36.53530473318713	39.0	35.0	41.0	31.0	41.0
51	35.63665212292885	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	8.0
9	31.0
10	31.0
11	40.0
12	30.0
13	23.0
14	30.0
15	34.0
16	69.0
17	118.0
18	215.0
19	341.0
20	618.0
21	921.0
22	1413.0
23	2151.0
24	3218.0
25	4593.0
26	5979.0
27	7101.0
28	8245.0
29	9902.0
30	12193.0
31	15499.0
32	20000.0
33	28375.0
34	58027.0
35	86444.0
36	69272.0
37	103244.0
38	190292.0
39	421580.0
40	586.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.118112664473685	21.76592196637427	25.448780914961013	10.667184454191034
2	32.537806103801174	23.5470539412768	25.86472420199805	18.050415752923975
3	31.91141645346004	24.204663133528264	26.796646564327485	17.087273848684212
4	29.47296083089669	25.528828581871345	25.684355202241715	19.31385538499025
5	24.84970836379142	30.618185002436647	24.599856846978557	19.93224978679337
6	26.553933662280706	35.008433083577	23.158237390350877	15.279395863791423
7	81.40657361720272	6.316341526559455	7.591678849902535	4.685406006335283
8	82.92110212597466	4.950772112573099	7.4614705165692	4.666655244883041
9	77.02517741837231	6.896472953216374	9.450193408869396	6.62815621954191
10	40.42369106359649	26.693374604044834	17.460956536306043	15.421977796052634
11	30.983872441520464	25.28906630726121	23.232478983918128	20.494582267300196
12	27.755314936647174	22.907814784356724	28.954316672758285	20.382553606237817
13	25.476669103313842	25.04283168859649	29.38510827850877	20.095390929580898
14	21.845208181042885	28.053613852339183	28.305178636695906	21.79599932992203
15	20.683803149366472	25.638001797027286	33.203410544590646	20.474784509015596
16	22.57020589668616	24.123378106725145	30.513866045321635	22.79254995126706
17	22.549646686159843	24.37970196759259	27.844214485867447	25.22643686038012
18	23.912075109649123	25.6235342044347	28.506868299220272	21.957522386695906
19	24.30565073708577	26.548793859649123	26.84062043128655	22.304934971978557
20	25.288685581140353	25.884997867933723	27.315290722465885	21.51102582846004
21	24.48506792153996	26.52214303118908	27.25684926291423	21.735939784356724
22	23.160616928606238	24.892635233918128	26.690423976608184	25.256323860867447
23	22.833192464668617	25.98950718810916	26.685188992446395	24.49211135477583
24	22.411347922758285	24.960309301900587	29.23519736842105	23.393145406920077
25	22.21013416788499	26.66615268640351	26.20842470760234	24.91528843810916
26	20.870168585526315	27.614255908869396	26.89544499269006	24.62013051291423
27	21.76715932626706	26.788175408138404	28.215232090643276	23.229433174951268
28	20.24006685550682	26.013207389132553	29.119551809210524	24.627173946150098
29	22.387266995614034	24.62803057992203	27.872197855750485	25.11250456871345
30	23.278166118421055	25.413468567251464	28.57387609649123	22.734489217836256
31	23.813371862816766	26.398882949561404	26.25382629751462	23.533918890107213
32	24.29946393762183	26.060798154239766	25.21986933479532	24.419868573343077
33	23.858583089668617	26.354718719541907	26.383463541666668	23.403234649122805
34	21.896986933479532	24.77927403143275	28.594244943957115	24.729494091130604
35	22.927231816520468	24.164115801656923	27.02460632919103	25.884046052631575
36	23.027648330896685	27.406664991471736	27.222298367446395	22.343388310185187
37	22.177106176900587	27.196599354288498	28.94127680311891	21.68501766569201
38	22.613323129873294	27.04002573708577	26.59114964059454	23.755501492446392
39	22.899629172758285	26.855278386939574	26.768663194444443	23.476429245857698
40	23.292157803362574	24.326685855263158	29.52140822977583	22.859748111598442
41	20.081779970760234	25.10603222465887	28.596338937621834	26.215848866959064
42	21.24737298976608	25.95724064936647	27.63367294103314	25.161713419834307
43	22.6377847831384	24.82791179337232	28.50030077363548	24.0340026498538
44	22.57306134259259	24.628887213693957	27.73846780579922	25.05958363791423
45	22.672335678606238	24.718167489035086	27.06363075657895	25.545866075779728
46	23.421890229044834	25.13553849902534	27.618348714668617	23.82422255726121
47	21.57489263523392	24.930327119883042	29.606690880847953	23.888089364035086
48	20.731203551413255	24.74224841617934	29.723097892300192	24.803450140107213
49	22.308456688596493	24.115097313596493	29.409569931773877	24.16687606603314
50	21.58165052387914	23.067814936647174	30.170070358187136	25.18046418128655
51	21.594024122807017	23.65280062134503	28.138706140350877	26.614469115497073
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	415.0
1	1154.5
2	1894.0
3	2768.5
4	3643.0
5	2639.5
6	1636.0
7	1665.0
8	1694.0
9	1723.0
10	1752.0
11	1831.5
12	1911.0
13	1964.0
14	2017.0
15	2080.0
16	2143.0
17	2330.0
18	2517.0
19	2569.0
20	2621.0
21	2787.0
22	2953.0
23	3077.0
24	3201.0
25	4473.5
26	6766.0
27	7786.0
28	10214.0
29	12642.0
30	14854.5
31	17067.0
32	18622.5
33	20178.0
34	22814.5
35	25451.0
36	29081.0
37	32711.0
38	33722.0
39	34733.0
40	40698.0
41	46663.0
42	51157.5
43	55652.0
44	62686.0
45	69720.0
46	77366.0
47	85012.0
48	94431.0
49	103850.0
50	100256.0
51	96662.0
52	87077.0
53	77492.0
54	68442.5
55	59393.0
56	54718.0
57	50043.0
58	47299.5
59	44556.0
60	42914.0
61	41272.0
62	39796.5
63	38321.0
64	34180.0
65	30039.0
66	25551.0
67	21063.0
68	18590.0
69	16117.0
70	13318.0
71	10519.0
72	9152.0
73	7785.0
74	6691.5
75	4206.5
76	2815.0
77	2296.0
78	1777.0
79	1332.5
80	888.0
81	611.0
82	334.0
83	285.0
84	236.0
85	154.0
86	72.0
87	46.0
88	20.0
89	14.0
90	8.0
91	5.0
92	2.0
93	3.0
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1050624.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.037170389365098
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.1208595120572	18.057022482308387
2	11.465497843823062	5.7412724622939235
3	4.211548760922161	3.163357917909828
4	2.08530121742355	2.088401675751356
5	1.2696128959848016	1.5893757202653371
6	0.835218717733059	1.254690800896178
7	0.5925105387169438	1.0384351120745439
8	0.4871459906931594	0.9757405738784558
9	0.3783779959670411	0.8526162920951976
>10	4.6689478944632175	29.11130927916492
>50	1.7490794842610606	29.743768559129048
>100	0.13015693043559523	4.914787032480249
>500	0.003828145012811613	0.6194521742447012
>1k	0.0019140725064058066	0.8497699175078943
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC	2501	0.2380490070662768	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1812	0.172468932748538	No Hit
CCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC	1794	0.17075566520467836	No Hit
CTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGCT	1666	0.15857242933723198	No Hit
TCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC	1093	0.10403341252436649	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.903630604288499E-4	0.0	0.0	0.16542549951267058	0.0
2	1.903630604288499E-4	0.0	0.0	0.779917458576998	0.0
3	2.8554459064327484E-4	0.0	0.0	1.0926839668615984	0.0
4	2.8554459064327484E-4	0.0	0.0	1.485022234405458	0.0
5	2.8554459064327484E-4	0.0	0.0	2.63586211622807	0.0
6	2.8554459064327484E-4	0.0	0.0	3.3198365923489277	0.0
7	2.8554459064327484E-4	0.0	0.0	3.9665950901559452	0.0
8	2.8554459064327484E-4	0.0	0.0	4.993032711988304	0.0
9	2.8554459064327484E-4	0.0	0.0	5.357387609649122	0.0
10	2.8554459064327484E-4	0.0	0.0	6.286835252192983	0.0
11	2.8554459064327484E-4	0.0	0.0	7.633558723196881	0.0
12	3.807261208576998E-4	0.0	0.0	8.645623933966862	0.0
13	4.7590765107212473E-4	0.0	0.0	9.021210252192983	0.0
14	4.7590765107212473E-4	0.0	0.0	9.164648818226121	0.0
15	4.7590765107212473E-4	0.0	0.0	9.450478953460038	0.0
16	4.7590765107212473E-4	0.0	0.0	10.103329069200779	0.0
17	4.7590765107212473E-4	0.0	0.0	10.928648117690058	0.0
18	4.7590765107212473E-4	0.0	0.0	11.81383634868421	0.0
19	4.7590765107212473E-4	0.0	0.0	12.295264528508772	0.0
20	4.7590765107212473E-4	0.0	0.0	12.775169803849902	0.0
21	5.710891812865497E-4	0.0	0.0	13.399465460526315	0.0
22	5.710891812865497E-4	0.0	0.0	14.076682048001949	0.0
23	5.710891812865497E-4	0.0	0.0	14.768366228070175	0.0
24	5.710891812865497E-4	0.0	0.0	15.326415539717349	0.0
25	5.710891812865497E-4	0.0	0.0	15.84201388888889	0.0
26	6.662707115009746E-4	0.0	0.0	16.275375395955166	0.0
27	6.662707115009746E-4	0.0	0.0	16.718159874512672	0.0
28	6.662707115009746E-4	0.0	0.0	17.157708181042885	9.518153021442495E-5
29	6.662707115009746E-4	0.0	0.0	17.626286854288498	9.518153021442495E-5
30	6.662707115009746E-4	0.0	0.0	18.183289169103315	9.518153021442495E-5
31	6.662707115009746E-4	0.0	0.0	18.677662037037038	9.518153021442495E-5
32	6.662707115009746E-4	0.0	0.0	19.139958729288498	9.518153021442495E-5
33	6.662707115009746E-4	0.0	0.0	19.597591526559455	9.518153021442495E-5
34	6.662707115009746E-4	0.0	0.0	20.049132705896685	9.518153021442495E-5
35	6.662707115009746E-4	0.0	0.0	20.555308083577	9.518153021442495E-5
36	6.662707115009746E-4	0.0	0.0	21.019032498781677	9.518153021442495E-5
37	7.614522417153996E-4	0.0	0.0	21.481234009502923	9.518153021442495E-5
38	7.614522417153996E-4	0.0	0.0	21.924875121832358	9.518153021442495E-5
39	7.614522417153996E-4	0.0	0.0	22.384506731237817	9.518153021442495E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTGCGT	25	3.8905764E-5	45.0	42
CGAAATT	20	7.0330093E-4	45.0	43
CCGTACT	20	7.0330093E-4	45.0	35
TGCGACG	40	6.8139343E-9	45.0	1
GCGATAT	90	0.0	45.0	9
AACTCGA	25	3.8905764E-5	45.0	12
TGGTCGA	20	7.0330093E-4	45.0	35
GTACGAG	65	0.0	44.999996	1
CGGTCTA	150	0.0	42.000004	31
CGTTCGG	260	0.0	40.673077	45
CACGACG	155	0.0	40.64516	26
ACGGTCT	155	0.0	40.64516	30
CACAACG	45	1.9279469E-8	40.0	12
GCCAACG	45	1.9279469E-8	40.0	1
CTCGTAG	40	3.4578443E-7	39.375	1
TAGTAAG	120	0.0	39.374996	1
CGTAAGG	195	0.0	39.23077	2
GCGTATG	75	0.0	39.000004	1
TTTCGCA	110	0.0	38.863636	14
GGCGATA	250	0.0	38.699997	8
>>END_MODULE