FastQCFastQC Report
Sat 18 Jun 2016
SRR3550245_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550245_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences795755
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13650.1715352087011706No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT11000.13823350151742686No Hit
GCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTGC9160.11511080671814818No Hit
CCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTGC8150.10241845794245717No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATCG302.1646556E-645.00000412
ACTCGAT207.031976E-445.00000441
CGTAATG207.031976E-445.00000422
TTACGCG207.031976E-445.00000416
CCGAATC302.1646556E-645.00000411
AGGCGTA253.8897197E-545.033
TCTAGCG351.2113014E-745.01
GTATGCG253.8897197E-545.01
CGTACAT253.8897197E-545.035
GCTACGA1500.042.00000410
TCGTTTA700.041.78571338
CATCGTT700.041.78571336
GTTGATG650.041.538461
TGCGTAG556.002665E-1140.9090921
CATGCGG950.040.2631572
TAACGCC900.040.012
TCTACGG451.9270374E-840.02
ATAACGC900.040.011
TAGGGCG2200.039.8863685
CACGGGT850.039.7058834