##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550245_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	795755
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.584036543911125	34.0	31.0	34.0	31.0	34.0
2	32.76382303598469	34.0	31.0	34.0	31.0	34.0
3	32.78839592588171	34.0	31.0	34.0	31.0	34.0
4	36.19382096248217	37.0	37.0	37.0	35.0	37.0
5	36.19603772517923	37.0	37.0	37.0	35.0	37.0
6	36.22237749055928	37.0	37.0	37.0	35.0	37.0
7	36.249136983116664	37.0	37.0	37.0	35.0	37.0
8	36.20653593128538	37.0	37.0	37.0	35.0	37.0
9	37.7390716992039	39.0	38.0	39.0	35.0	39.0
10	37.667885215927015	39.0	37.0	39.0	35.0	39.0
11	37.59983034979359	39.0	37.0	39.0	35.0	39.0
12	37.39850205151083	39.0	37.0	39.0	35.0	39.0
13	37.33660108953133	39.0	37.0	39.0	35.0	39.0
14	38.632398162751095	40.0	38.0	41.0	35.0	41.0
15	38.71451263265703	40.0	38.0	41.0	35.0	41.0
16	38.77491815948376	40.0	38.0	41.0	35.0	41.0
17	38.76656257265113	40.0	38.0	41.0	35.0	41.0
18	38.72149845115645	40.0	38.0	41.0	35.0	41.0
19	38.67457823073685	40.0	38.0	41.0	35.0	41.0
20	38.58809432551476	40.0	38.0	41.0	35.0	41.0
21	38.51698072899322	40.0	37.0	41.0	35.0	41.0
22	38.44895225289191	40.0	37.0	41.0	35.0	41.0
23	38.368005227739694	40.0	37.0	41.0	34.0	41.0
24	38.30156015356485	40.0	37.0	41.0	34.0	41.0
25	38.29807289932203	40.0	37.0	41.0	34.0	41.0
26	38.23197089556459	40.0	37.0	41.0	34.0	41.0
27	38.187716068387886	40.0	36.0	41.0	34.0	41.0
28	38.11809790701912	40.0	36.0	41.0	34.0	41.0
29	38.07971046364773	40.0	36.0	41.0	34.0	41.0
30	38.00992642207715	40.0	36.0	41.0	34.0	41.0
31	37.88164070599619	40.0	36.0	41.0	34.0	41.0
32	37.78098912353677	40.0	36.0	41.0	33.0	41.0
33	37.694550458369726	40.0	35.0	41.0	33.0	41.0
34	37.58791462196279	40.0	35.0	41.0	33.0	41.0
35	37.51349850142318	40.0	35.0	41.0	33.0	41.0
36	37.43379055111184	40.0	35.0	41.0	33.0	41.0
37	37.3507021633543	40.0	35.0	41.0	33.0	41.0
38	37.24808515183693	40.0	35.0	41.0	33.0	41.0
39	37.170705807692066	39.0	35.0	41.0	33.0	41.0
40	37.0632204635849	39.0	35.0	41.0	32.0	41.0
41	36.97694265194689	39.0	35.0	41.0	32.0	41.0
42	36.87386067319715	39.0	35.0	41.0	32.0	41.0
43	36.80467731902407	39.0	35.0	41.0	32.0	41.0
44	36.70894433588227	39.0	35.0	41.0	32.0	41.0
45	36.59073584206194	39.0	35.0	41.0	31.0	41.0
46	36.51758644306351	39.0	35.0	41.0	31.0	41.0
47	36.45191044982438	38.0	35.0	41.0	31.0	41.0
48	36.41094557998379	38.0	35.0	41.0	31.0	41.0
49	36.296812461121824	38.0	35.0	41.0	31.0	41.0
50	36.185655132547076	38.0	35.0	40.0	31.0	41.0
51	35.29273582949526	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	9.0
9	19.0
10	32.0
11	46.0
12	20.0
13	15.0
14	25.0
15	47.0
16	77.0
17	93.0
18	172.0
19	316.0
20	505.0
21	869.0
22	1180.0
23	1815.0
24	2802.0
25	3729.0
26	4988.0
27	5977.0
28	6554.0
29	7615.0
30	9189.0
31	11624.0
32	14785.0
33	21677.0
34	46033.0
35	70835.0
36	53472.0
37	80107.0
38	139843.0
39	310785.0
40	495.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.41568070574486	21.24875118598061	22.852636804041445	11.482931304233086
2	32.066276680636626	25.151459934276254	23.99834119798179	18.78392218710533
3	32.04453632085252	24.995884411659368	25.299621114539022	17.659958152949088
4	29.142009789445243	26.575013666266628	24.647912988294134	19.63506355599399
5	25.75013666266627	31.44460292426689	23.359702420971278	19.44555799209556
6	27.571802879026837	35.91042469101671	21.94130102858292	14.576471401373539
7	82.06558551312904	6.425721484627807	6.982048494825668	4.526644507417484
8	83.92470044171887	5.627988514052692	6.345546053747698	4.1017649904807385
9	78.03117793793317	7.182612738845499	8.402837556785695	6.3833717664356495
10	42.63799787623075	25.63565419004593	15.790538545155231	15.935809388568089
11	33.77811009670062	24.645776652361594	21.307186257076612	20.268926993861175
12	30.06289624319043	22.488957028231052	26.63558507329517	20.812561655283346
13	25.71972529233244	24.51558582729609	28.018548422567246	21.746140457804223
14	21.601874948947856	28.21446299426331	27.584746561441648	22.598915495347185
15	21.89028029984103	24.83100954439495	31.694051561096064	21.584658594667957
16	23.860736030562173	24.028626901496065	29.91146772561907	22.1991693423227
17	23.988036518777765	23.27073031272188	27.352388612072808	25.388844556427543
18	24.760887459079743	24.449862080665532	27.574818882696306	23.21443157755842
19	26.55189097146735	25.78293570257177	25.13964725323749	22.52552607272339
20	28.697400581837375	24.303837236335305	24.955545362580192	22.04321681924713
21	26.559430980641025	25.385451552299386	26.145358810186554	21.90975865687303
22	25.351898511476524	24.635597639977128	25.279640090228778	24.73286375831757
23	25.36320852523704	25.438357283334696	25.573826114821774	23.624608076606492
24	23.804939962676954	24.550898203592812	27.62269794094916	24.02146389278107
25	24.722307745474424	25.331791820346716	25.660284886679946	24.285615547498914
26	23.500574925699492	26.417176140897634	25.46437031498388	24.617878618418988
27	23.717601523081854	26.233953917977264	26.662100772222608	23.386343786718275
28	21.536025535497735	27.17218239282191	27.33140225320608	23.96038981847428
29	23.472928225396007	26.52059993339659	26.9072767371867	23.09919510402071
30	25.025667447895394	25.644702201054344	26.32631903035482	23.003311320695442
31	25.744230322146894	27.38305131604577	23.96818116128708	22.904537200520263
32	25.76886101878091	27.54126584187344	24.240752492915533	22.44912064643012
33	25.763708679178894	26.613216379413263	24.434279395039933	23.188795546367917
34	23.410597482893607	26.981797161186545	26.05022902777865	23.5573763281412
35	24.443327406048343	26.120602446732978	25.645204868332588	23.790865278886088
36	25.346243504596266	29.042858668811377	23.697369165132486	21.91352866145987
37	24.462680096260783	28.57097976135871	24.42058171170775	22.54575843067276
38	24.017065554096423	28.270007728509405	24.699059383855584	23.01386733353859
39	25.2214563527719	25.65211654340846	25.196825656137882	23.929601447681762
40	25.655509547536614	24.385395002230588	27.271459180275336	22.687636269957462
41	22.441706304076003	26.156794490766632	26.67278245188532	24.72871675327205
42	24.506537816287675	25.830689094005066	25.367606863921683	24.295166225785575
43	24.15316271968131	26.11620410804833	25.738072647988385	23.992560524281973
44	23.699254167425902	25.851047118773995	26.042563351785414	24.40713536201469
45	23.52646229052912	26.050480361417772	25.657017549371353	24.766039798681756
46	24.153665386959556	26.67278245188532	25.541278408555396	23.632273752599733
47	23.666329460700844	26.234079584796827	27.320594906723805	22.778996047778524
48	23.93450245364465	25.09277352954113	26.96370113916972	24.0090228776445
49	23.0384980301726	25.340714164535566	28.014652751160845	23.606135054130984
50	22.44798964505407	25.54555108042048	26.9968771795339	25.00958209499155
51	22.131057926120477	25.5997134796514	26.11708377578526	26.152144818442864
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	485.0
1	1116.0
2	1747.0
3	2390.5
4	3034.0
5	2194.0
6	1354.0
7	1381.5
8	1409.0
9	1524.0
10	1639.0
11	1704.5
12	1770.0
13	1704.5
14	1639.0
15	1612.0
16	1585.0
17	1652.5
18	1720.0
19	1887.0
20	2054.0
21	2198.0
22	2342.0
23	2587.0
24	2832.0
25	3181.5
26	4232.0
27	4933.0
28	6506.5
29	8080.0
30	9699.5
31	11319.0
32	13139.0
33	14959.0
34	16746.5
35	18534.0
36	20593.5
37	22653.0
38	23311.5
39	23970.0
40	26747.5
41	29525.0
42	33752.5
43	37980.0
44	42435.5
45	46891.0
46	50963.5
47	55036.0
48	58863.0
49	62690.0
50	62527.5
51	62365.0
52	57877.5
53	53390.0
54	50715.5
55	48041.0
56	47109.5
57	46178.0
58	45133.0
59	44088.0
60	43954.0
61	43820.0
62	39760.0
63	35700.0
64	32486.5
65	29273.0
66	25062.5
67	20852.0
68	17685.0
69	14518.0
70	13424.5
71	12331.0
72	10319.5
73	8308.0
74	6783.0
75	4489.0
76	3720.0
77	3009.0
78	2298.0
79	1549.5
80	801.0
81	825.0
82	849.0
83	471.5
84	94.0
85	115.0
86	136.0
87	73.0
88	10.0
89	9.5
90	9.0
91	6.5
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	795755.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.64164940401535
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.3268348199186	16.60240485898742
2	8.810616813358667	3.989737938423797
3	3.2093868750958197	2.1799743728330387
4	1.7003280430506589	1.5399292569027412
5	1.069130439313658	1.2103438287050374
6	0.7990081351098676	1.0854517239668249
7	0.6108390462272755	0.9681282468872766
8	0.5309091386235953	0.9616526865682583
9	0.46816862141072674	0.954009880914858
>10	7.894530546125253	45.71813035903167
>50	1.5053966317281862	21.18421864894651
>100	0.07094076891631813	2.8504643007418062
>500	0.0027929436581227616	0.4437962211067086
>1k	0.0011171774632491047	0.3117576759840515
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1365	0.1715352087011706	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1100	0.13823350151742686	No Hit
GCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTGC	916	0.11511080671814818	No Hit
CCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTGC	815	0.10241845794245717	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.5133363912259427E-4	0.0	0.0	0.06383874433713894	0.0
2	2.5133363912259427E-4	0.0	0.0	0.367952447675478	0.0
3	2.5133363912259427E-4	0.0	0.0	0.5334556490377064	0.0
4	2.5133363912259427E-4	0.0	0.0	0.7546292514655892	0.0
5	2.5133363912259427E-4	0.0	0.0	1.3561963167055187	0.0
6	2.5133363912259427E-4	0.0	0.0	1.733573775848094	0.0
7	2.5133363912259427E-4	0.0	0.0	2.071114853189738	0.0
8	2.5133363912259427E-4	0.0	0.0	2.586600147030179	0.0
9	2.5133363912259427E-4	0.0	0.0	2.795081400682371	0.0
10	2.5133363912259427E-4	0.0	0.0	3.289454668836514	0.0
11	3.770004586838914E-4	0.0	0.0	4.024479896450541	0.0
12	3.770004586838914E-4	0.0	0.0	4.6283089644425734	0.0
13	3.770004586838914E-4	0.0	0.0	4.853378238276856	0.0
14	3.770004586838914E-4	0.0	0.0	4.94184767924801	0.0
15	3.770004586838914E-4	0.0	0.0	5.114388222505671	0.0
16	3.770004586838914E-4	0.0	0.0	5.481461002444219	0.0
17	3.770004586838914E-4	0.0	0.0	5.915891197667624	0.0
18	3.770004586838914E-4	0.0	0.0	6.420820478664916	1.2566681956129714E-4
19	3.770004586838914E-4	0.0	0.0	6.7097284968363375	1.2566681956129714E-4
20	3.770004586838914E-4	0.0	0.0	6.9941125095035535	1.2566681956129714E-4
21	3.770004586838914E-4	0.0	0.0	7.339947596936243	1.2566681956129714E-4
22	3.770004586838914E-4	0.0	0.0	7.694830695377346	1.2566681956129714E-4
23	5.026672782451885E-4	0.0	0.0	8.052101463390114	1.2566681956129714E-4
24	5.026672782451885E-4	0.0	0.0	8.336988143335574	1.2566681956129714E-4
25	5.026672782451885E-4	0.0	0.0	8.593851122518865	1.2566681956129714E-4
26	5.026672782451885E-4	0.0	0.0	8.831361411489718	1.2566681956129714E-4
27	5.026672782451885E-4	0.0	0.0	9.07289303868653	1.2566681956129714E-4
28	5.026672782451885E-4	0.0	0.0	9.32271867597439	1.2566681956129714E-4
29	5.026672782451885E-4	0.0	0.0	9.588378332526972	1.2566681956129714E-4
30	5.026672782451885E-4	0.0	0.0	9.897016041369517	1.2566681956129714E-4
31	5.026672782451885E-4	0.0	0.0	10.178007049908578	1.2566681956129714E-4
32	5.026672782451885E-4	0.0	0.0	10.450201381078347	1.2566681956129714E-4
33	5.026672782451885E-4	0.0	0.0	10.731318056436969	1.2566681956129714E-4
34	5.026672782451885E-4	0.0	0.0	11.00464338898279	1.2566681956129714E-4
35	6.283340978064857E-4	0.0	0.0	11.318810437886032	1.2566681956129714E-4
36	6.283340978064857E-4	0.0	0.0	11.591130435875364	1.2566681956129714E-4
37	6.283340978064857E-4	0.0	0.0	11.874760447625212	1.2566681956129714E-4
38	6.283340978064857E-4	0.0	0.0	12.162663131240143	1.2566681956129714E-4
39	6.283340978064857E-4	0.0	0.0	12.473060175556547	1.2566681956129714E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATCG	30	2.1646556E-6	45.000004	12
ACTCGAT	20	7.031976E-4	45.000004	41
CGTAATG	20	7.031976E-4	45.000004	22
TTACGCG	20	7.031976E-4	45.000004	16
CCGAATC	30	2.1646556E-6	45.000004	11
AGGCGTA	25	3.8897197E-5	45.0	33
TCTAGCG	35	1.2113014E-7	45.0	1
GTATGCG	25	3.8897197E-5	45.0	1
CGTACAT	25	3.8897197E-5	45.0	35
GCTACGA	150	0.0	42.000004	10
TCGTTTA	70	0.0	41.785713	38
CATCGTT	70	0.0	41.785713	36
GTTGATG	65	0.0	41.53846	1
TGCGTAG	55	6.002665E-11	40.909092	1
CATGCGG	95	0.0	40.263157	2
TAACGCC	90	0.0	40.0	12
TCTACGG	45	1.9270374E-8	40.0	2
ATAACGC	90	0.0	40.0	11
TAGGGCG	220	0.0	39.886368	5
CACGGGT	85	0.0	39.705883	4
>>END_MODULE