FastQCFastQC Report
Sat 18 Jun 2016
SRR3550243_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550243_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences680455
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGC40230.5912220499518704TruSeq Adapter, Index 13 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGC27380.4023778207228986TruSeq Adapter, Index 13 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGCT22260.3271340500106546TruSeq Adapter, Index 13 (100% over 25bp)
TCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGC14870.21853024814278682TruSeq Adapter, Index 13 (96% over 25bp)
ACTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGC10840.15930517080482912TruSeq Adapter, Index 13 (100% over 24bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT8190.12036064104165595No Hit
GCCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTG7970.11712751026886421TruSeq Adapter, Index 13 (95% over 24bp)
ACCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTG7660.11257173508902132TruSeq Adapter, Index 13 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTCG253.889121E-545.00000442
TTGGCCG253.889121E-545.0000041
ACGTTAG453.8380676E-1045.0000041
CTATCGA253.889121E-545.0000042
CCGGCAC253.889121E-545.00000436
TTAGATC253.889121E-545.00000428
CGTTGAT453.8380676E-1045.00000425
ATCGTTG453.8380676E-1045.00000423
TACGCGG351.2109558E-745.0000042
ACCTACG351.2109558E-745.00000431
CGCTATG351.2109558E-745.00000437
CACTACG253.889121E-545.0000041
GCCTCGT351.2109558E-745.00000441
CGCGTGA351.2109558E-745.00000414
GCTCGAT253.889121E-545.0000049
GCGTAAG750.045.0000041
GTACGCC253.889121E-545.00000410
AGGTCGC351.2109558E-745.00000411
ACGCGAT207.0312555E-445.038
TCGTTAC207.0312555E-445.020
Produced by FastQC (version 0.11.5)