##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550243_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	680455
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.60683513237466	34.0	31.0	34.0	31.0	34.0
2	32.78384610297522	34.0	31.0	34.0	31.0	34.0
3	32.79124409402532	34.0	31.0	34.0	31.0	34.0
4	36.21129391363132	37.0	37.0	37.0	35.0	37.0
5	36.2135879668751	37.0	35.0	37.0	35.0	37.0
6	36.23331594300872	37.0	36.0	37.0	35.0	37.0
7	36.34459883460332	37.0	37.0	37.0	35.0	37.0
8	36.283244299770004	37.0	37.0	37.0	35.0	37.0
9	38.094567605499265	39.0	39.0	39.0	37.0	39.0
10	37.84567825939996	39.0	38.0	39.0	35.0	39.0
11	37.66437163368629	39.0	37.0	39.0	35.0	39.0
12	37.36271171495543	39.0	37.0	39.0	35.0	39.0
13	37.04814866523135	39.0	37.0	39.0	33.0	39.0
14	38.246915666723	40.0	37.0	41.0	34.0	41.0
15	38.39855096957183	40.0	37.0	41.0	34.0	41.0
16	38.477637757089006	40.0	37.0	41.0	35.0	41.0
17	38.452284133410735	40.0	37.0	41.0	35.0	41.0
18	38.41164955801633	40.0	37.0	41.0	35.0	41.0
19	38.43124820891903	40.0	37.0	41.0	35.0	41.0
20	38.383159797488446	40.0	36.0	41.0	35.0	41.0
21	38.34263985127598	40.0	36.0	41.0	34.0	41.0
22	38.296470743840516	40.0	36.0	41.0	34.0	41.0
23	38.28081504287572	40.0	36.0	41.0	34.0	41.0
24	38.25483389790655	40.0	36.0	41.0	34.0	41.0
25	38.181674026937856	40.0	36.0	41.0	34.0	41.0
26	38.09125658566694	40.0	36.0	41.0	34.0	41.0
27	38.025380076566414	40.0	35.0	41.0	34.0	41.0
28	37.966316655767095	40.0	36.0	41.0	34.0	41.0
29	37.88194957785599	40.0	36.0	41.0	34.0	41.0
30	37.822936123623165	40.0	36.0	41.0	34.0	41.0
31	37.703088374690466	40.0	35.0	41.0	34.0	41.0
32	37.56809928650682	40.0	35.0	41.0	34.0	41.0
33	37.50512671668222	40.0	35.0	41.0	33.0	41.0
34	37.42108883026798	40.0	35.0	41.0	33.0	41.0
35	37.335691559324275	40.0	35.0	41.0	33.0	41.0
36	37.265497351037176	40.0	35.0	41.0	33.0	41.0
37	37.15960790941355	39.0	35.0	41.0	33.0	41.0
38	37.058312452697095	39.0	35.0	41.0	33.0	41.0
39	36.99856860483059	39.0	35.0	41.0	32.0	41.0
40	36.88675224665849	39.0	35.0	41.0	32.0	41.0
41	36.82324327104658	39.0	35.0	41.0	32.0	41.0
42	36.77526067116855	39.0	35.0	41.0	32.0	41.0
43	36.65585380370487	39.0	35.0	41.0	31.0	41.0
44	36.54368327075266	39.0	35.0	41.0	31.0	41.0
45	36.44112542342991	39.0	35.0	41.0	31.0	41.0
46	36.35515500657648	38.0	35.0	41.0	31.0	41.0
47	36.25667090402745	38.0	35.0	40.0	31.0	41.0
48	36.24370751923345	38.0	35.0	40.0	31.0	41.0
49	36.17079454188741	38.0	35.0	40.0	31.0	41.0
50	36.04528734449743	38.0	35.0	40.0	30.0	41.0
51	34.54080578436487	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	12.0
10	19.0
11	12.0
12	12.0
13	18.0
14	14.0
15	18.0
16	39.0
17	79.0
18	164.0
19	247.0
20	453.0
21	705.0
22	1086.0
23	1532.0
24	2201.0
25	3201.0
26	4161.0
27	5006.0
28	5669.0
29	6720.0
30	8212.0
31	10233.0
32	13417.0
33	20111.0
34	46683.0
35	72760.0
36	39838.0
37	63179.0
38	128110.0
39	246396.0
40	144.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.74198881630674	21.930766913315356	24.86453916864451	10.4627051017334
2	31.95758720268056	24.060665290136747	25.662975509034396	18.318771998148296
3	32.006965927210466	23.655495220110073	26.96871945977324	17.368819392906218
4	29.30568516654298	25.185500878088924	25.500878088925795	20.007935866442306
5	25.70147915732855	28.69682785783042	25.608600127855624	19.9930928569854
6	27.048519005665327	33.20586960195752	24.413517425840062	15.332093966537098
7	79.27901183766744	6.298285705887972	8.96106281826131	5.461639638183274
8	79.45022080813573	5.236496168005232	9.198844890551175	6.114438133307861
9	73.78944970644642	7.192099404075214	11.61267093341955	7.405779956058814
10	46.30548676988192	21.23490899471677	18.159319866853796	14.300284368547517
11	34.34231506859381	24.24759903299998	22.763885929267914	18.646199969138298
12	33.11460713787098	21.150847594624185	27.27204591045697	18.462499357047857
13	24.694946763562616	26.744751673512575	28.424950951936573	20.135350610988237
14	17.96018840334776	31.010132925762907	28.18584623523965	22.843832435649674
15	17.894937945933236	26.59147188278431	36.779654789809754	18.73393538147269
16	20.254388607622843	24.892167740702913	34.85212100726719	20.00132264440705
17	19.65302628388358	25.44562094480899	27.53686871284655	27.36448405846088
18	21.09235731973459	27.006047424150015	30.23300585637551	21.66858939973988
19	24.138260428683747	28.44170444775922	25.826542534039724	21.593492589517307
20	27.183281774694873	26.63614787164471	26.546501972944576	19.634068380715846
21	24.415574872695476	27.216053963891806	27.945565834625363	20.422805328787355
22	21.1305670470494	26.964016724103722	26.407918231183547	25.497497997663327
23	22.174133484212767	26.86981505022375	27.090108824242602	23.86594264132088
24	24.01834066911111	24.636162567693674	28.419072532349677	22.926424230845537
25	20.865156402701135	27.223255027885752	27.04793116370664	24.863657405706476
26	19.19465651659551	30.999257849527158	27.157563689002213	22.648521944875117
27	20.843112329250282	30.195090050040047	26.401745890617306	22.560051730092365
28	18.07070269158137	29.164897017436864	28.60292010492979	24.161480186051982
29	19.257996487644295	28.28739593360325	28.47153742716271	23.983070151589743
30	20.196045293222916	29.090828930641997	25.620503927519085	25.092621848615998
31	21.52060018663982	29.445150671242036	22.730966779581312	26.303282362536834
32	22.485542761828484	30.635971519057104	23.793344159422738	23.085141559691678
33	22.66424671727006	28.622024968587194	23.48531497306949	25.228413341073253
34	18.748190548970907	28.101784835147072	24.68157335900243	28.46845125687959
35	18.199293120044675	27.911471001021376	26.772086324591633	27.117149554342312
36	20.43985274558935	28.131323893571214	25.203723978808295	26.225099382031143
37	19.8974215782087	27.900889845764965	27.286448038444867	24.91524053758147
38	19.449926887156387	29.289813433658363	24.677164544312262	26.58309513487299
39	20.87162266424672	27.463976309969063	26.776201218302457	24.888199807481758
40	22.402510085163605	24.903483698407683	28.041384073891734	24.652622142536977
41	17.955632628167915	26.51314194178895	27.356695152508248	28.17453027753489
42	20.022191033940526	25.53747125085421	26.66553996957918	27.77479774562609
43	22.8883614640204	24.774599348965033	25.53394419910207	26.803094987912502
44	20.264822802389578	27.07071003960585	24.86644965501025	27.79801750299432
45	19.98001337340456	27.3192202276418	24.28830708863922	28.412459310314418
46	21.090005951899833	27.595946829694835	25.767023535722423	25.547023682682912
47	18.148885672087058	26.458178718651492	29.642812529851348	25.750123079410102
48	19.768390268276374	22.640733038922487	28.972672696945427	28.618203995855712
49	19.28959299292385	23.330565577444503	30.258430021088827	27.121411408542812
50	18.658397689781104	24.28154690611429	29.24851753606043	27.811537868044176
51	17.708739005518368	24.924352087941156	25.580971555797223	31.785937350743254
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	364.0
1	878.0
2	1392.0
3	1693.5
4	1995.0
5	1600.5
6	1206.0
7	1115.0
8	1024.0
9	1024.0
10	1024.0
11	1099.0
12	1174.0
13	1309.5
14	1445.0
15	1506.0
16	1567.0
17	1454.5
18	1342.0
19	1449.5
20	1557.0
21	1925.5
22	2294.0
23	2326.0
24	2358.0
25	2781.0
26	4160.5
27	5117.0
28	6419.5
29	7722.0
30	8334.5
31	8947.0
32	10315.0
33	11683.0
34	13370.5
35	15058.0
36	16349.0
37	17640.0
38	18933.0
39	20226.0
40	24254.5
41	28283.0
42	34130.0
43	39977.0
44	44639.5
45	49302.0
46	56895.0
47	64488.0
48	71169.0
49	77850.0
50	76732.5
51	75615.0
52	62735.5
53	49856.0
54	44118.5
55	38381.0
56	33745.0
57	29109.0
58	28028.0
59	26947.0
60	26657.5
61	26368.0
62	24528.5
63	22689.0
64	19082.5
65	15476.0
66	12411.5
67	9347.0
68	7999.5
69	6652.0
70	5460.5
71	4269.0
72	3410.0
73	2551.0
74	2304.0
75	1697.5
76	1338.0
77	1018.0
78	698.0
79	473.0
80	248.0
81	310.0
82	372.0
83	264.0
84	156.0
85	106.5
86	57.0
87	34.0
88	11.0
89	10.0
90	9.0
91	7.0
92	5.0
93	3.0
94	1.0
95	2.5
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	680455.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.940627015395187
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.74319728033483	15.978739919034346
2	13.356802107324429	6.395404347397971
3	5.825194447062103	4.18376422647795
4	3.1195566124878154	2.9873656525192196
5	1.8909267553795288	2.2634986081986352
6	1.191782777876938	1.7119216181114005
7	0.8070753408398621	1.3525322795859056
8	0.6049622444974375	1.1586540363127569
9	0.4722946074008874	1.0176326133450435
>10	4.031315028101832	23.183417961168544
>50	1.692798859047589	28.859517192353223
>100	0.25667209590966367	8.495348705236262
>500	0.004329408846669026	0.7008137034524912
>1k	0.0030924348904778757	1.7113891368062315
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGC	4023	0.5912220499518704	TruSeq Adapter, Index 13 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGC	2738	0.4023778207228986	TruSeq Adapter, Index 13 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGCT	2226	0.3271340500106546	TruSeq Adapter, Index 13 (100% over 25bp)
TCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGC	1487	0.21853024814278682	TruSeq Adapter, Index 13 (96% over 25bp)
ACTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGC	1084	0.15930517080482912	TruSeq Adapter, Index 13 (100% over 24bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	819	0.12036064104165595	No Hit
GCCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTG	797	0.11712751026886421	TruSeq Adapter, Index 13 (95% over 24bp)
ACCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTG	766	0.11257173508902132	TruSeq Adapter, Index 13 (100% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.469604896723516E-4	0.0	0.0	0.35593830598643555	0.0
2	1.469604896723516E-4	0.0	0.0	1.8246614397719174	0.0
3	1.469604896723516E-4	0.0	0.0	2.585622855295354	0.0
4	1.469604896723516E-4	0.0	0.0	3.3896436942927894	0.0
5	1.469604896723516E-4	0.0	0.0	5.644605447825352	0.0
6	1.469604896723516E-4	0.0	0.0	6.660543312930319	0.0
7	1.469604896723516E-4	0.0	0.0	7.784497137944464	0.0
8	1.469604896723516E-4	0.0	0.0	9.403413892175088	0.0
9	1.469604896723516E-4	0.0	0.0	9.928503721774401	0.0
10	1.469604896723516E-4	0.0	0.0	11.271722597379695	0.0
11	1.469604896723516E-4	0.0	0.0	13.343571580780507	0.0
12	1.469604896723516E-4	0.0	0.0	14.830223894306016	0.0
13	1.469604896723516E-4	0.0	0.0	15.411158709980821	0.0
14	1.469604896723516E-4	0.0	0.0	15.656288806754304	0.0
15	1.469604896723516E-4	0.0	0.0	16.11539337649073	0.0
16	1.469604896723516E-4	0.0	0.0	17.04580023660639	0.0
17	1.469604896723516E-4	0.0	0.0	18.130074729409	0.0
18	4.4088146901705475E-4	0.0	0.0	19.291062597820577	0.0
19	4.4088146901705475E-4	0.0	0.0	20.036005319969725	0.0
20	4.4088146901705475E-4	0.0	0.0	20.7893247900302	0.0
21	4.4088146901705475E-4	0.0	0.0	21.706652166565018	0.0
22	4.4088146901705475E-4	0.0	0.0	22.657486534745132	0.0
23	4.4088146901705475E-4	0.0	0.0	23.54454005040745	0.0
24	5.878419586894064E-4	0.0	0.0	24.253036571117853	0.0
25	5.878419586894064E-4	0.0	0.0	24.86277564276844	0.0
26	5.878419586894064E-4	0.0	0.0	25.407117296514834	0.0
27	5.878419586894064E-4	0.0	0.0	25.94763797752974	0.0
28	5.878419586894064E-4	0.0	0.0	26.49006914491039	0.0
29	5.878419586894064E-4	0.0	0.0	27.05630791161796	0.0
30	5.878419586894064E-4	0.0	0.0	27.71425002388108	0.0
31	5.878419586894064E-4	0.0	0.0	28.371310373206164	0.0
32	5.878419586894064E-4	0.0	0.0	28.979579839960028	0.0
33	5.878419586894064E-4	0.0	0.0	29.530093834272655	0.0
34	0.0011756839173788127	0.0	0.0	30.061943846396897	0.0
35	0.0011756839173788127	0.0	0.0	30.665804498460588	0.0
36	0.0011756839173788127	0.0	0.0	31.249237642459825	0.0
37	0.0011756839173788127	0.0	0.0	31.817827777002154	0.0
38	0.0011756839173788127	0.0	0.0	32.37319146747397	0.0
39	0.0011756839173788127	0.0	0.0	32.92341154080725	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTTTCG	25	3.889121E-5	45.000004	42
TTGGCCG	25	3.889121E-5	45.000004	1
ACGTTAG	45	3.8380676E-10	45.000004	1
CTATCGA	25	3.889121E-5	45.000004	2
CCGGCAC	25	3.889121E-5	45.000004	36
TTAGATC	25	3.889121E-5	45.000004	28
CGTTGAT	45	3.8380676E-10	45.000004	25
ATCGTTG	45	3.8380676E-10	45.000004	23
TACGCGG	35	1.2109558E-7	45.000004	2
ACCTACG	35	1.2109558E-7	45.000004	31
CGCTATG	35	1.2109558E-7	45.000004	37
CACTACG	25	3.889121E-5	45.000004	1
GCCTCGT	35	1.2109558E-7	45.000004	41
CGCGTGA	35	1.2109558E-7	45.000004	14
GCTCGAT	25	3.889121E-5	45.000004	9
GCGTAAG	75	0.0	45.000004	1
GTACGCC	25	3.889121E-5	45.000004	10
AGGTCGC	35	1.2109558E-7	45.000004	11
ACGCGAT	20	7.0312555E-4	45.0	38
TCGTTAC	20	7.0312555E-4	45.0	20
>>END_MODULE