SRR3550239_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550239_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1003323
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGC	1994	0.19873958834791985	TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1428	0.14232704722208103	No Hit
CCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGC	1350	0.13455288077717742	TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGCT	1104	0.11003435583555844	TruSeq Adapter, Index 23 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1059	0.10554925980965253	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGA	20	7.032856E-4	45.000004	22
ATATGCG	35	1.2117198E-7	45.000004	1
CACGAAA	20	7.032856E-4	45.000004	32
CGCTACC	20	7.032856E-4	45.000004	41
GTATACG	20	7.032856E-4	45.000004	1
TATAGCG	20	7.032856E-4	45.000004	1
TCGAACG	20	7.032856E-4	45.000004	3
ACGGTTC	20	7.032856E-4	45.000004	32
TCACGCA	25	3.89045E-5	45.0	40
TGTCACG	25	3.89045E-5	45.0	32
TCGATAG	50	2.1827873E-11	45.0	1
TCTAGCG	25	3.89045E-5	45.0	1
ATATCGT	25	3.89045E-5	45.0	27
TCTATCG	25	3.89045E-5	45.0	24
TATCCGT	25	3.89045E-5	45.0	43
GCTACGA	165	0.0	42.272728	10
AACACGT	70	0.0	41.785717	41
GATAGCG	120	0.0	41.249996	1
TGCGATG	55	6.002665E-11	40.909092	1
GTATGCG	50	1.0804797E-9	40.5	1