##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550239_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1003323
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.112675579050816	33.0	31.0	34.0	30.0	34.0
2	32.272164597044025	34.0	31.0	34.0	30.0	34.0
3	32.292090383655115	34.0	31.0	34.0	30.0	34.0
4	35.852384526219375	37.0	35.0	37.0	35.0	37.0
5	35.85425032616615	37.0	35.0	37.0	35.0	37.0
6	35.72998226892038	37.0	35.0	37.0	35.0	37.0
7	36.02293379101246	37.0	35.0	37.0	35.0	37.0
8	36.02462816062225	37.0	35.0	37.0	35.0	37.0
9	37.641864085643405	39.0	38.0	39.0	35.0	39.0
10	37.29248307872938	39.0	37.0	39.0	34.0	39.0
11	37.15994849116386	39.0	37.0	39.0	34.0	39.0
12	36.940146891878285	39.0	37.0	39.0	33.0	39.0
13	36.836598981584196	39.0	35.0	39.0	33.0	39.0
14	37.974765853070245	40.0	37.0	41.0	33.0	41.0
15	38.0078259942212	40.0	37.0	41.0	33.0	41.0
16	38.08088521841919	40.0	37.0	41.0	33.0	41.0
17	38.05861721499457	40.0	37.0	41.0	33.0	41.0
18	37.98320979385502	40.0	36.0	41.0	33.0	41.0
19	37.91780912029326	40.0	36.0	41.0	33.0	41.0
20	37.77499768270039	40.0	36.0	41.0	33.0	41.0
21	37.63002642219903	39.0	36.0	41.0	33.0	41.0
22	37.623345622496444	39.0	36.0	41.0	33.0	41.0
23	37.61946451940203	39.0	36.0	41.0	33.0	41.0
24	37.65507717853573	39.0	36.0	41.0	33.0	41.0
25	37.56467159628554	39.0	35.0	41.0	33.0	41.0
26	37.496117401873576	39.0	35.0	41.0	33.0	41.0
27	37.429164885086855	39.0	35.0	41.0	33.0	41.0
28	37.36091468051664	39.0	35.0	41.0	32.0	41.0
29	37.29789110784862	39.0	35.0	41.0	32.0	41.0
30	37.20059542141464	39.0	35.0	41.0	32.0	41.0
31	37.08820788519749	39.0	35.0	41.0	32.0	41.0
32	36.96474814192438	39.0	35.0	41.0	31.0	41.0
33	36.81041798104897	39.0	35.0	41.0	31.0	41.0
34	36.70080223417583	39.0	35.0	41.0	31.0	41.0
35	36.617238915085174	39.0	35.0	41.0	30.0	41.0
36	36.54099527270879	39.0	35.0	41.0	30.0	41.0
37	36.51077170562222	39.0	35.0	41.0	30.0	41.0
38	36.39420405990892	39.0	35.0	41.0	30.0	41.0
39	36.29691535029098	39.0	35.0	41.0	30.0	41.0
40	36.14235993792627	38.0	35.0	40.0	30.0	41.0
41	36.097113292528924	38.0	35.0	40.0	30.0	41.0
42	36.02521122310562	38.0	35.0	40.0	29.0	41.0
43	35.95331214374633	38.0	35.0	40.0	29.0	41.0
44	35.84927087288939	38.0	35.0	40.0	29.0	41.0
45	35.665858352693995	38.0	34.0	40.0	28.0	41.0
46	35.61568109173217	38.0	34.0	40.0	28.0	41.0
47	35.55395022340762	38.0	34.0	40.0	28.0	41.0
48	35.469041375509185	38.0	34.0	40.0	28.0	41.0
49	35.36228213646054	37.0	34.0	40.0	28.0	41.0
50	35.21942684459541	37.0	34.0	40.0	28.0	41.0
51	33.80977711066127	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	8.0
9	21.0
10	31.0
11	30.0
12	22.0
13	21.0
14	28.0
15	48.0
16	68.0
17	180.0
18	312.0
19	610.0
20	975.0
21	1535.0
22	2344.0
23	3412.0
24	4746.0
25	6865.0
26	8663.0
27	10407.0
28	12634.0
29	15290.0
30	18640.0
31	23579.0
32	29603.0
33	40975.0
34	71467.0
35	87366.0
36	80844.0
37	114802.0
38	189921.0
39	277798.0
40	77.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.975293101025294	20.576025866047125	25.329629640703942	11.119051392223641
2	31.339458977816715	24.67111787530038	25.40109217071671	18.5883309761662
3	31.883650628959963	24.16818910759546	26.563330054229795	17.38483020921478
4	28.763120151735784	26.01485264466179	26.363294771474386	18.85873243212804
5	25.575612240524737	30.03020961345449	24.440783277169963	19.95339486885081
6	26.877286776043206	34.7199256869423	23.53359785433006	14.86918968268444
7	81.33083762656692	6.36395258555819	7.761508507230473	4.543701280644418
8	82.9043089812553	5.274871601667659	7.441571657382518	4.379247759694535
9	76.26467249330476	7.721740655800774	9.709335876881124	6.304250974013353
10	42.39203128005637	25.599233746261174	16.966619921999197	15.042115051683256
11	33.559083166637265	25.19258503991237	22.18059388651511	19.067737906935253
12	30.076057261719306	22.814587126977052	26.904695696201525	20.204659915102116
13	26.220469380249433	24.204269213403858	28.69674073055237	20.878520675794334
14	21.86145438707176	26.83333283498933	29.01528221719227	22.28993056074664
15	21.07486821292844	25.283383317236822	32.73342682266827	20.908321647166467
16	24.127424568160006	24.550618295404373	30.450712283083313	20.871244853352312
17	23.953602179956007	24.495501448686017	27.71450470087898	23.836391670479
18	24.947300121695605	25.286572718855243	28.379893613522267	21.386233545926885
19	26.117411840454167	27.528722056605897	25.49119276643713	20.8626733365028
20	28.394046583204013	25.45750471184255	26.275287220566057	19.87316148438738
21	26.10844164840236	26.95173937007325	26.703165381437483	20.236653600086914
22	25.555778149210173	24.86467468601836	26.50313009868208	23.076417066089387
23	24.515136202399425	25.91169543606595	27.124864076673216	22.448304284861408
24	25.32245348706249	23.823534395204735	27.84038639600607	23.0136257217267
25	23.649712007000737	26.062494331336968	26.12777739571404	24.160016265948254
26	22.832926186282982	27.54945316712564	26.84718679826935	22.770433848322025
27	23.423862504896228	26.49206686181818	26.9269218387299	23.15714879455569
28	21.192776404009475	27.596995185000246	27.354401324399024	23.85582708659126
29	24.55709676744179	26.621038289763117	26.517781412366702	22.304083530428386
30	24.510252431171217	25.888970949534695	27.033567455345885	22.567209163948203
31	25.071387778412337	26.938483419596682	25.69152705559426	22.298601746396724
32	27.098551513321233	26.631702851424716	24.637031145503492	21.63271448975056
33	24.614306658972236	27.745601366658594	25.392121978664896	22.247969995704274
34	22.866315234475838	27.271377213519475	27.846765199242917	22.01554235276177
35	23.86320257783386	27.63028456439252	26.593430032003653	21.913082825769965
36	23.02967239861939	30.13625721726702	24.753743310977622	22.08032707313597
37	24.089650092741817	28.41716974493757	25.486807339211797	22.006372823108812
38	23.137414372041707	29.086146734401584	24.750155234156896	23.026283659399816
39	24.242542032824925	26.511502277930436	26.17312669997598	23.07282898926866
40	24.216129800672366	25.3785670217866	27.677627244665974	22.727675932875055
41	21.046661942365517	27.69915570559032	26.30458984793531	24.949592504108846
42	22.758473592252944	26.669277989241753	26.082228753850956	24.49001966465435
43	24.010413396284147	25.16906320297651	25.9803672396626	24.84015616107674
44	23.044323712304013	25.883987509505914	26.258941537271646	24.81274724091843
45	23.458248241094843	25.110956292240882	26.055019171293793	25.375776295370482
46	22.89282713542897	26.99330125991331	25.955350370718104	24.15852123393962
47	21.53254734517199	27.10124257093678	27.434136364859572	23.932073719031656
48	21.98006025975683	25.666011842646885	28.124442477646777	24.22948541994951
49	22.354216937117958	25.258565785893477	28.617703371695857	23.769513905292712
50	21.480520231271484	25.869635202223012	27.58503492893116	25.064809637574342
51	21.173939000700674	25.54620994435491	26.80054179959993	26.47930925534449
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	232.0
1	1022.5
2	1813.0
3	2571.5
4	3330.0
5	2570.0
6	1810.0
7	1705.0
8	1600.0
9	1614.5
10	1629.0
11	1697.0
12	1765.0
13	1817.5
14	1870.0
15	1863.5
16	1857.0
17	2073.0
18	2289.0
19	2401.5
20	2514.0
21	2953.0
22	3392.0
23	3336.5
24	3281.0
25	4383.0
26	6275.5
27	7066.0
28	9309.0
29	11552.0
30	13492.0
31	15432.0
32	18982.5
33	22533.0
34	24602.0
35	26671.0
36	29350.0
37	32029.0
38	33562.0
39	35095.0
40	38872.5
41	42650.0
42	48863.0
43	55076.0
44	59827.0
45	64578.0
46	69913.0
47	75248.0
48	79770.0
49	84292.0
50	83115.0
51	81938.0
52	73845.5
53	65753.0
54	61042.0
55	56331.0
56	55388.0
57	54445.0
58	52068.5
59	49692.0
60	49702.5
61	49713.0
62	46986.5
63	44260.0
64	37300.0
65	30340.0
66	26005.5
67	21671.0
68	18508.5
69	15346.0
70	12511.0
71	9676.0
72	8352.5
73	7029.0
74	5892.0
75	3919.0
76	3083.0
77	2538.0
78	1993.0
79	1583.5
80	1174.0
81	925.5
82	677.0
83	427.5
84	178.0
85	116.0
86	54.0
87	66.0
88	78.0
89	54.5
90	31.0
91	20.5
92	10.0
93	7.0
94	4.0
95	3.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1003323.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.716432803531085
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.69229044272974	16.74025963980566
2	10.357864146031897	4.705874497228749
3	3.7222452322528423	2.5366840109020696
4	1.8296220790002788	1.662499480538667
5	1.0832520112354236	1.2303810761259697
6	0.6888546988643225	0.9388992886888813
7	0.5035881469013394	0.8007808409817312
8	0.3856558678176212	0.7008580485253166
9	0.3230448435325781	0.6604583832571521
>10	5.122966729245091	29.658861306677924
>50	2.0564187093597996	32.621402339060936
>100	0.23021264343117484	6.7618857882206145
>500	0.0017708664879321143	0.2837063449427443
>1k	0.002213583109915143	0.6974489550435774
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGC	1994	0.19873958834791985	TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1428	0.14232704722208103	No Hit
CCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGC	1350	0.13455288077717742	TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGCT	1104	0.11003435583555844	TruSeq Adapter, Index 23 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1059	0.10554925980965253	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.99006401727061E-4	0.0	0.0	0.12628037032939543	0.0
2	2.99006401727061E-4	0.0	0.0	0.640671050100516	0.0
3	2.99006401727061E-4	0.0	0.0	0.9192453477095611	0.0
4	3.98675202302748E-4	0.0	0.0	1.2248298902746175	0.0
5	4.98344002878435E-4	0.0	0.0	2.1323143195162477	0.0
6	4.98344002878435E-4	0.0	0.0	2.625475544764747	0.0
7	4.98344002878435E-4	0.0	0.0	3.1392682117324133	0.0
8	4.98344002878435E-4	0.0	0.0	3.9765858053687597	0.0
9	4.98344002878435E-4	0.0	0.0	4.286954450361449	0.0
10	4.98344002878435E-4	0.0	0.0	4.948256942181132	0.0
11	4.98344002878435E-4	0.0	0.0	5.84886422418304	0.0
12	5.98012803454122E-4	0.0	0.0	6.539768349773702	0.0
13	5.98012803454122E-4	0.0	0.0	6.821332711400018	0.0
14	6.976816040298089E-4	0.0	0.0	6.936450176064937	0.0
15	6.976816040298089E-4	0.0	0.0	7.173960927836799	0.0
16	6.976816040298089E-4	0.0	0.0	7.657553948230031	0.0
17	6.976816040298089E-4	0.0	0.0	8.214204199445243	0.0
18	6.976816040298089E-4	0.0	0.0	8.854077899141155	0.0
19	6.976816040298089E-4	0.0	0.0	9.224048486878104	0.0
20	6.976816040298089E-4	0.0	0.0	9.593321393011024	0.0
21	6.976816040298089E-4	0.0	0.0	10.068841240557626	0.0
22	6.976816040298089E-4	0.0	0.0	10.552733267352588	0.0
23	6.976816040298089E-4	0.0	0.0	11.036425956546397	0.0
24	6.976816040298089E-4	0.0	0.0	11.44536704530844	0.0
25	6.976816040298089E-4	0.0	0.0	11.78025421524275	0.0
26	6.976816040298089E-4	0.0	0.0	12.11693542358742	0.0
27	6.976816040298089E-4	0.0	0.0	12.410759047684545	0.0
28	6.976816040298089E-4	0.0	0.0	12.754417072069513	0.0
29	6.976816040298089E-4	0.0	0.0	13.116812830962711	0.0
30	7.97350404605496E-4	0.0	0.0	13.506617510014223	0.0
31	7.97350404605496E-4	0.0	0.0	13.874395384138507	0.0
32	7.97350404605496E-4	0.0	0.0	14.237289487034584	0.0
33	7.97350404605496E-4	0.0	0.0	14.608157093976715	0.0
34	7.97350404605496E-4	0.0	0.0	14.976233974502728	0.0
35	8.970192051811829E-4	0.0	0.0	15.357168130303004	0.0
36	9.9668800575687E-4	0.0	0.0	15.723351303618077	0.0
37	9.9668800575687E-4	0.0	0.0	16.107674198637927	0.0
38	9.9668800575687E-4	0.0	0.0	16.501465629712467	0.0
39	9.9668800575687E-4	0.0	0.0	16.915290489702716	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGA	20	7.032856E-4	45.000004	22
ATATGCG	35	1.2117198E-7	45.000004	1
CACGAAA	20	7.032856E-4	45.000004	32
CGCTACC	20	7.032856E-4	45.000004	41
GTATACG	20	7.032856E-4	45.000004	1
TATAGCG	20	7.032856E-4	45.000004	1
TCGAACG	20	7.032856E-4	45.000004	3
ACGGTTC	20	7.032856E-4	45.000004	32
TCACGCA	25	3.89045E-5	45.0	40
TGTCACG	25	3.89045E-5	45.0	32
TCGATAG	50	2.1827873E-11	45.0	1
TCTAGCG	25	3.89045E-5	45.0	1
ATATCGT	25	3.89045E-5	45.0	27
TCTATCG	25	3.89045E-5	45.0	24
TATCCGT	25	3.89045E-5	45.0	43
GCTACGA	165	0.0	42.272728	10
AACACGT	70	0.0	41.785717	41
GATAGCG	120	0.0	41.249996	1
TGCGATG	55	6.002665E-11	40.909092	1
GTATGCG	50	1.0804797E-9	40.5	1
>>END_MODULE