FastQCFastQC Report
Sat 18 Jun 2016
SRR3550237_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550237_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences791848
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13300.16796152797001446No Hit
GCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGC11030.13929441003829018No Hit
CCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGC9640.12174053606247663No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG600.045.0000042
GATCGCA253.889703E-545.035
TTCGTAA207.031956E-445.034
AATGCGA207.031956E-445.025
GCGATAT253.889703E-545.09
TACTACG253.889703E-545.021
ACGTAGT207.031956E-445.041
TAGTACG451.9270374E-840.01
GATTACG403.4564073E-739.3751
TATGGGT1150.039.1304324
GTAACGG700.038.571432
TACGTCA356.2458257E-638.5714343
GCGAAAC301.1396011E-437.50000435
ACTTCGT301.1396011E-437.50000438
GTTAGCG601.546141E-1037.5000041
CGACAAT301.1396011E-437.50000420
AAACGTC301.1396011E-437.50000414
TCGTAAG301.1396011E-437.5000041
CAGTACG301.1396011E-437.5000041
ATCGGAA301.1396011E-437.50000428