##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550237_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	791848
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.17799249350885	33.0	31.0	34.0	30.0	34.0
2	32.34402562107879	34.0	31.0	34.0	30.0	34.0
3	32.34896217455876	34.0	31.0	34.0	30.0	34.0
4	35.909031783877715	37.0	35.0	37.0	35.0	37.0
5	35.901266404663524	37.0	35.0	37.0	35.0	37.0
6	35.77682585546721	37.0	35.0	37.0	35.0	37.0
7	36.03919691657995	37.0	35.0	37.0	35.0	37.0
8	36.03834069164789	37.0	35.0	37.0	35.0	37.0
9	37.65103277396672	39.0	38.0	39.0	35.0	39.0
10	37.31516402137784	39.0	37.0	39.0	34.0	39.0
11	37.184040371384405	39.0	37.0	39.0	34.0	39.0
12	37.01810953617361	39.0	37.0	39.0	33.0	39.0
13	36.94114400743577	39.0	37.0	39.0	33.0	39.0
14	38.10709757428193	40.0	37.0	41.0	33.0	41.0
15	38.16006102181227	40.0	37.0	41.0	33.0	41.0
16	38.23341095765854	40.0	37.0	41.0	34.0	41.0
17	38.20654721613239	40.0	37.0	41.0	34.0	41.0
18	38.15533536739374	40.0	37.0	41.0	34.0	41.0
19	38.08369788141158	40.0	37.0	41.0	34.0	41.0
20	37.92716152594942	40.0	36.0	41.0	33.0	41.0
21	37.78291919661349	40.0	36.0	41.0	33.0	41.0
22	37.77470044756064	40.0	36.0	41.0	33.0	41.0
23	37.74642355603601	40.0	36.0	41.0	33.0	41.0
24	37.757700972913995	40.0	36.0	41.0	33.0	41.0
25	37.66506829593558	40.0	36.0	41.0	33.0	41.0
26	37.588074984087854	40.0	36.0	41.0	33.0	41.0
27	37.510148412321556	40.0	36.0	41.0	33.0	41.0
28	37.430737969913416	39.0	35.0	41.0	33.0	41.0
29	37.37743859932714	39.0	35.0	41.0	32.0	41.0
30	37.31356901829644	39.0	35.0	41.0	32.0	41.0
31	37.20033769107202	39.0	35.0	41.0	32.0	41.0
32	37.076543730614965	39.0	35.0	41.0	31.0	41.0
33	36.94757327163799	39.0	35.0	41.0	31.0	41.0
34	36.827859639728835	39.0	35.0	41.0	31.0	41.0
35	36.747817763005024	39.0	35.0	41.0	31.0	41.0
36	36.60643330538184	39.0	35.0	41.0	30.0	41.0
37	36.56998312807509	39.0	35.0	41.0	30.0	41.0
38	36.43080363908225	39.0	35.0	41.0	30.0	41.0
39	36.331568684899125	39.0	35.0	41.0	30.0	41.0
40	36.172732645659266	38.0	35.0	40.0	30.0	41.0
41	36.09294965700488	38.0	35.0	40.0	30.0	41.0
42	35.96449697416676	38.0	35.0	40.0	30.0	41.0
43	35.90445641082632	38.0	35.0	40.0	29.0	41.0
44	35.82758686010447	38.0	35.0	40.0	29.0	41.0
45	35.69550974429436	38.0	34.0	40.0	29.0	41.0
46	35.64441155373253	38.0	34.0	40.0	29.0	41.0
47	35.57319965447914	38.0	34.0	40.0	28.0	41.0
48	35.483861801759936	38.0	34.0	40.0	28.0	41.0
49	35.37487623887413	37.0	34.0	40.0	28.0	41.0
50	35.24561153150605	37.0	34.0	40.0	28.0	41.0
51	33.8654539760156	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	11.0
9	11.0
10	25.0
11	15.0
12	9.0
13	17.0
14	31.0
15	37.0
16	67.0
17	154.0
18	265.0
19	483.0
20	817.0
21	1197.0
22	1792.0
23	2579.0
24	3760.0
25	4979.0
26	6463.0
27	7967.0
28	9567.0
29	11399.0
30	14047.0
31	17788.0
32	22730.0
33	31601.0
34	53135.0
35	65293.0
36	66503.0
37	95476.0
38	153959.0
39	219605.0
40	65.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.50512724664329	21.200786009436154	25.222643739707625	11.07144300421293
2	31.464119376445986	24.803245067235125	25.536719168325234	18.195916387993655
3	32.324764348713394	24.272082520887846	26.63200513229812	16.77114799810065
4	29.08916862832261	26.12609995857791	25.7663086855053	19.018422727594185
5	25.67904446307877	30.35494185752821	23.964447722290135	20.00156595710288
6	26.716743617461937	35.130984734443985	23.26595508228852	14.886316565805558
7	83.03676968307049	6.087405663713238	6.813302553015226	4.062522100201049
8	84.46911528475162	4.906370919671453	6.514255261110718	4.110258534466211
9	77.82579484951657	7.808948181974317	8.58523352966731	5.7800234388417975
10	43.26082783564523	25.560966246047222	15.899010921287923	15.27919499701963
11	33.14310322183045	26.241273577757347	21.46409411907336	19.151529081338843
12	30.05791515543387	22.506971034845073	26.232180923611605	21.202932886109455
13	25.7770430688718	23.746097735929116	28.09213384386903	22.384725351330054
14	21.432774977015793	26.858311191036666	28.734176256049142	22.974737575898406
15	21.28337761792667	25.275936795950738	31.588511936634305	21.852173649488286
16	23.867838271991594	23.802674250613755	30.40444630787727	21.92504116951738
17	23.49516573887918	24.096922641719118	28.010299956557322	24.397611662844383
18	24.575802426728362	24.58615794950546	27.574862852466637	23.26317677129954
19	27.478632262757497	25.52851052222144	24.84163627362827	22.151220941392793
20	29.14233539770259	24.243415402956124	24.900233378123072	21.71401582121821
21	26.238116406178964	25.880093149190248	25.8041947444459	22.077595700184887
22	25.57940412806498	24.477172386619657	24.939634879421302	25.003788605894062
23	26.123447934452066	25.737515280710436	25.05619765409523	23.082839130742265
24	24.96388195714329	23.28300380881179	27.64103716874956	24.11207706529536
25	24.72570493327002	26.1110718218648	25.115299906042576	24.047923338822603
26	23.88829674381952	27.85067336155424	25.493276487406675	22.76775340721957
27	24.69943726573787	27.009602853072813	25.385806366878494	22.90515351431083
28	23.342106060759136	26.942165668158534	26.865509542235376	22.85021872884695
29	24.788722077974562	26.541710025156345	25.85129974439539	22.818268152473706
30	24.97802608581445	26.109935240096583	25.606934664228486	23.30510400986048
31	24.913240925026013	27.32595144522686	24.34949636798982	23.411311261757305
32	26.40898253200109	27.440619916953757	23.920247320192765	22.230150230852384
33	26.139865226659666	26.898091552924296	24.084546529131853	22.877496691284186
34	24.93786686333741	26.649559006273932	26.25668057505986	22.155893555328802
35	26.081773269617404	25.770728725715035	25.110248431517164	23.037249573150405
36	25.385301219425948	28.068518200462716	24.577065295359716	21.96911528475162
37	26.073943484103008	27.20294804053303	25.40954829714794	21.313560178216022
38	25.753048564876085	26.654863054525617	24.84226770794395	22.749820672654348
39	26.39029207625706	24.935467412937836	25.876178256433054	22.798062254372052
40	27.015790909366444	24.59979693072408	27.085627544680293	21.298784615229184
41	23.152928339782385	26.262994918216627	26.880411392085353	23.70366534991564
42	24.5720138208343	26.349501419464342	26.11776502561098	22.960719734090382
43	24.998737131368646	26.17876158050535	26.082909851385622	22.739591436740383
44	23.97871813782443	25.910275709479595	26.18015073599984	23.930855416696133
45	24.336993968539417	25.24954284155545	25.887038926662694	24.52642426324244
46	24.14491164971055	26.61735585617442	24.817389195906284	24.420343298208746
47	24.368818258049522	26.33763045432962	26.64476010547479	22.64879118214607
48	24.702847011042522	24.787711783069476	26.77155211606268	23.73788908982532
49	24.02759115385781	25.973166567320998	27.07969206211293	22.919550216708256
50	23.00870874208181	25.38315434275265	26.970706499227127	24.637430415938415
51	23.845106636627232	25.55048443640699	25.304730200745595	25.299678726220183
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	364.0
1	833.0
2	1302.0
3	2046.5
4	2791.0
5	2140.0
6	1489.0
7	1367.0
8	1245.0
9	1243.0
10	1241.0
11	1311.0
12	1381.0
13	1307.5
14	1234.0
15	1290.5
16	1347.0
17	1463.5
18	1580.0
19	1596.0
20	1612.0
21	2010.5
22	2409.0
23	2613.5
24	2818.0
25	3210.0
26	4847.5
27	6093.0
28	7325.5
29	8558.0
30	9894.0
31	11230.0
32	13354.0
33	15478.0
34	17367.0
35	19256.0
36	21564.5
37	23873.0
38	24454.0
39	25035.0
40	29379.5
41	33724.0
42	36822.0
43	39920.0
44	43607.5
45	47295.0
46	51110.0
47	54925.0
48	57981.5
49	61038.0
50	59871.5
51	58705.0
52	54432.5
53	50160.0
54	47308.0
55	44456.0
56	43916.5
57	43377.0
58	43064.5
59	42752.0
60	42662.0
61	42572.0
62	39869.5
63	37167.0
64	32972.0
65	28777.0
66	24495.0
67	20213.0
68	18136.0
69	16059.0
70	14380.5
71	12702.0
72	10624.0
73	8546.0
74	7449.0
75	5280.5
76	4209.0
77	3206.5
78	2204.0
79	1887.0
80	1570.0
81	1166.5
82	763.0
83	502.5
84	242.0
85	175.0
86	108.0
87	71.0
88	34.0
89	26.0
90	18.0
91	14.5
92	11.0
93	7.0
94	3.0
95	4.5
96	6.0
97	4.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	791848.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.808876600153667
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.48815005609913	17.734791728593933
2	8.687703246483622	4.136889090685659
3	3.042287448229797	2.1730033931129884
4	1.4972625440627934	1.4259255659849275
5	1.022075896083719	1.2167239442924376
6	0.6680144144009598	0.9542803655757819
7	0.5827439658804	0.9712135421191422
8	0.5028219381492163	0.9577300381795831
9	0.4113337706179366	0.8814055487506529
>10	7.630437069464134	45.690604624499045
>50	1.4104105326611387	21.22276898466021
>100	0.05408180098662292	2.0274387132921525
>500	0.0016063901283155325	0.29704660768224334
>1k	0.001070926752210355	0.31017785257120084
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1330	0.16796152797001446	No Hit
GCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGC	1103	0.13929441003829018	No Hit
CCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGC	964	0.12174053606247663	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.7886058940604764E-4	0.0	0.0	0.1024186460027682	0.0
2	3.7886058940604764E-4	0.0	0.0	0.498706822521494	0.0
3	3.7886058940604764E-4	0.0	0.0	0.7099847445469333	0.0
4	3.7886058940604764E-4	0.0	0.0	0.9647053474909326	0.0
5	3.7886058940604764E-4	0.0	0.0	1.6880764995302129	0.0
6	3.7886058940604764E-4	0.0	0.0	2.1352582818924843	0.0
7	3.7886058940604764E-4	0.0	0.0	2.5709479597094393	0.0
8	3.7886058940604764E-4	0.0	0.0	3.2839635889716208	0.0
9	3.7886058940604764E-4	0.0	0.0	3.5814954385185036	0.0
10	3.7886058940604764E-4	0.0	0.0	4.188935250199533	0.0
11	3.7886058940604764E-4	0.0	0.0	4.952339337852719	0.0
12	3.7886058940604764E-4	0.0	0.0	5.547908184399026	0.0
13	5.051474525413969E-4	0.0	0.0	5.789621240440084	0.0
14	5.051474525413969E-4	0.0	0.0	5.884336387791596	0.0
15	6.314343156767461E-4	0.0	0.0	6.069220355421748	0.0
16	6.314343156767461E-4	0.0	0.0	6.447323223648983	0.0
17	6.314343156767461E-4	0.0	0.0	6.901577070346835	0.0
18	6.314343156767461E-4	0.0	0.0	7.411523423687374	0.0
19	6.314343156767461E-4	0.0	0.0	7.707792404602904	0.0
20	6.314343156767461E-4	0.0	0.0	8.005450541012921	0.0
21	6.314343156767461E-4	0.0	0.0	8.369535567432134	0.0
22	6.314343156767461E-4	0.0	0.0	8.745617845849203	0.0
23	6.314343156767461E-4	0.0	0.0	9.135717966074298	0.0
24	6.314343156767461E-4	0.0	0.0	9.450298542144452	0.0
25	6.314343156767461E-4	0.0	0.0	9.720426142390965	0.0
26	6.314343156767461E-4	0.0	0.0	9.98095594103919	0.0
27	6.314343156767461E-4	0.0	0.0	10.227089037289986	0.0
28	7.577211788120953E-4	0.0	0.0	10.493933179094977	0.0
29	7.577211788120953E-4	0.0	0.0	10.786413554116447	0.0
30	7.577211788120953E-4	0.0	0.0	11.112486234731918	0.0
31	7.577211788120953E-4	0.0	0.0	11.420247320192765	0.0
32	7.577211788120953E-4	0.0	0.0	11.7168951616977	0.0
33	7.577211788120953E-4	0.0	0.0	11.996999424131904	0.0
34	7.577211788120953E-4	0.0	0.0	12.279629423828816	0.0
35	7.577211788120953E-4	0.0	0.0	12.578929289459593	0.0
36	8.840080419474445E-4	0.0	0.0	12.883280629615784	0.0
37	8.840080419474445E-4	0.0	0.0	13.180686192299532	0.0
38	8.840080419474445E-4	0.0	0.0	13.475692304583708	0.0
39	8.840080419474445E-4	0.0	0.0	13.793177478505976	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTACGG	60	0.0	45.000004	2
GATCGCA	25	3.889703E-5	45.0	35
TTCGTAA	20	7.031956E-4	45.0	34
AATGCGA	20	7.031956E-4	45.0	25
GCGATAT	25	3.889703E-5	45.0	9
TACTACG	25	3.889703E-5	45.0	21
ACGTAGT	20	7.031956E-4	45.0	41
TAGTACG	45	1.9270374E-8	40.0	1
GATTACG	40	3.4564073E-7	39.375	1
TATGGGT	115	0.0	39.130432	4
GTAACGG	70	0.0	38.57143	2
TACGTCA	35	6.2458257E-6	38.57143	43
GCGAAAC	30	1.1396011E-4	37.500004	35
ACTTCGT	30	1.1396011E-4	37.500004	38
GTTAGCG	60	1.546141E-10	37.500004	1
CGACAAT	30	1.1396011E-4	37.500004	20
AAACGTC	30	1.1396011E-4	37.500004	14
TCGTAAG	30	1.1396011E-4	37.500004	1
CAGTACG	30	1.1396011E-4	37.500004	1
ATCGGAA	30	1.1396011E-4	37.500004	28
>>END_MODULE