##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3550236_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 912656 Sequences flagged as poor quality 0 Sequence length 51 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.151112796058975 33.0 31.0 34.0 30.0 34.0 2 32.326774819866415 34.0 31.0 34.0 30.0 34.0 3 32.3307960502095 34.0 31.0 34.0 30.0 34.0 4 35.87979917953753 37.0 35.0 37.0 35.0 37.0 5 35.884691493837764 37.0 35.0 37.0 35.0 37.0 6 35.7505215546712 37.0 35.0 37.0 35.0 37.0 7 36.019404901737346 37.0 35.0 37.0 35.0 37.0 8 36.02183407548956 37.0 35.0 37.0 35.0 37.0 9 37.65417638190074 39.0 38.0 39.0 35.0 39.0 10 37.30401597096825 39.0 37.0 39.0 34.0 39.0 11 37.15406133307621 39.0 37.0 39.0 34.0 39.0 12 36.936411966830875 39.0 37.0 39.0 33.0 39.0 13 36.8404952139689 39.0 37.0 39.0 33.0 39.0 14 37.9998224960993 40.0 37.0 41.0 33.0 41.0 15 38.0592873985379 40.0 37.0 41.0 33.0 41.0 16 38.12575274802335 40.0 37.0 41.0 33.0 41.0 17 38.10062389333988 40.0 37.0 41.0 33.0 41.0 18 38.0484848617661 40.0 37.0 41.0 33.0 41.0 19 37.95398814010974 40.0 36.0 41.0 33.0 41.0 20 37.80879104503778 40.0 36.0 41.0 33.0 41.0 21 37.69037293350397 40.0 36.0 41.0 33.0 41.0 22 37.66656768815413 40.0 36.0 41.0 33.0 41.0 23 37.640794560053294 40.0 36.0 41.0 33.0 41.0 24 37.66086564926982 40.0 36.0 41.0 33.0 41.0 25 37.574674357041424 39.0 35.0 41.0 33.0 41.0 26 37.448905173471715 39.0 35.0 41.0 33.0 41.0 27 37.38763674374572 39.0 35.0 41.0 32.0 41.0 28 37.322337222348835 39.0 35.0 41.0 32.0 41.0 29 37.227283883522375 39.0 35.0 41.0 32.0 41.0 30 37.1856975684157 39.0 35.0 41.0 32.0 41.0 31 37.0677177381182 39.0 35.0 41.0 32.0 41.0 32 36.94466261110429 39.0 35.0 41.0 31.0 41.0 33 36.826263126523024 39.0 35.0 41.0 31.0 41.0 34 36.71529579600638 39.0 35.0 41.0 31.0 41.0 35 36.633335013411404 39.0 35.0 41.0 31.0 41.0 36 36.529532485405234 39.0 35.0 41.0 31.0 41.0 37 36.4422783611788 39.0 35.0 41.0 30.0 41.0 38 36.30466462719798 39.0 35.0 41.0 30.0 41.0 39 36.20570839396224 38.0 35.0 40.0 30.0 41.0 40 36.05190455111236 38.0 35.0 40.0 30.0 41.0 41 35.986054986763904 38.0 35.0 40.0 30.0 41.0 42 35.89825958521064 38.0 35.0 40.0 29.0 41.0 43 35.83563248365211 38.0 35.0 40.0 29.0 41.0 44 35.76271453866517 38.0 35.0 40.0 29.0 41.0 45 35.602253203835836 38.0 34.0 40.0 29.0 41.0 46 35.560419259830645 38.0 34.0 40.0 29.0 41.0 47 35.48310097123122 38.0 34.0 40.0 28.0 41.0 48 35.39506122788871 37.0 34.0 40.0 28.0 41.0 49 35.3189745095633 37.0 34.0 40.0 28.0 41.0 50 35.16022137585246 37.0 34.0 40.0 27.0 41.0 51 33.80684945916096 35.0 32.0 39.0 24.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 10.0 9 27.0 10 24.0 11 31.0 12 25.0 13 20.0 14 24.0 15 55.0 16 94.0 17 185.0 18 378.0 19 611.0 20 963.0 21 1504.0 22 2366.0 23 3205.0 24 4582.0 25 6153.0 26 7918.0 27 9628.0 28 11114.0 29 13473.0 30 16653.0 31 20849.0 32 26957.0 33 36592.0 34 63622.0 35 81047.0 36 76719.0 37 107374.0 38 171370.0 39 249003.0 40 78.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.210269805929066 21.34440577829982 25.53086814747287 11.914456268298242 2 30.314269560491574 25.41198436212549 26.240883750284883 18.03286232709805 3 31.839488262828493 24.506276187303868 26.274740975789346 17.379494574078294 4 28.05153310776459 25.854977121719465 26.76594467137673 19.327545099139215 5 26.109508949702846 30.61361564488701 23.54731684227135 19.729558563138795 6 26.621421433705578 35.73274048491436 22.374585824231694 15.271252257148369 7 82.5135648042636 5.978703914728003 7.287849967567188 4.219881313441209 8 84.56855595098263 5.034646131729809 6.525569327325958 3.871228589961607 9 78.3232674742729 7.408158166932558 8.439105205027962 5.829469153766588 10 43.93802265037429 25.77038884311285 15.626807910099751 14.664780596413108 11 33.16046790904788 24.965485352641082 22.027467085079152 19.846579653231885 12 30.21105432934205 22.064063568310512 26.517767921319752 21.20711418102768 13 25.253874406128922 25.049197068775094 28.47644676636104 21.220481758734945 14 21.62501533984327 27.29757981101313 27.87446748829789 23.202937360845706 15 21.44477218141337 24.880897074034465 31.81450623235918 21.859824512192986 16 23.625769183569716 24.031946319314176 29.870838519661298 22.471445977454813 17 23.618975824407006 23.98910432846549 27.67263897898003 24.71928086814747 18 24.181948072439123 24.680492978734595 27.51266632772918 23.624892621097104 19 26.753672796760224 25.550590803106537 25.164574604232044 22.531161795901195 20 28.264647358917273 25.050292771865852 25.23524745358602 21.449812415630863 21 26.29051910029628 26.159801721568698 25.84763591101138 21.702043267123646 22 25.56428709174103 24.601931067127154 24.739880086253745 25.09390175487807 23 24.735606844199786 25.731600953699967 25.475096860153222 24.05769534194702 24 24.455545134201714 23.61240160586245 28.131519433390018 23.800533826545816 25 24.332497677109448 26.265537069826966 25.92641696323697 23.475548289826616 26 23.49801020318718 28.000582914044287 24.7077759856945 23.793630897074035 27 24.367779316631896 26.615066355779177 25.791535912764502 23.225618414824424 28 22.50946687470416 26.743701898634313 27.265694851072038 23.481136375589486 29 22.98708385196613 26.57222436493049 26.122766957101035 24.31792482600235 30 24.16616994793219 25.323232411773986 26.25731929664627 24.25327834364755 31 24.573990638312793 26.888225136305465 24.16123928402377 24.37654494135797 32 25.702455251485773 27.51956925720096 24.09462053610561 22.68335495520766 33 25.269104679090475 26.640705808102943 23.46491996984625 24.625269542960325 34 22.976565102294842 27.048636068792625 26.059873599691453 23.914925229221087 35 23.573504146140493 26.680479830297504 24.882211917743376 24.86380410581862 36 24.994850195473433 26.978072789747724 24.20167072807279 23.825406286706052 37 23.97990042250311 27.960589751231566 25.03473378797707 23.02477603828825 38 23.801519959327504 28.05503935765502 24.245389281394086 23.898051401623395 39 25.045581248575587 26.160459143423154 24.19871232972774 24.595247278273522 40 25.250916007783875 25.68426658017917 26.222804649287358 22.8420127627496 41 22.270932311845865 25.795918725127542 26.009799968443755 25.923348994582845 42 23.06487877141004 26.696367525113512 25.95698707946915 24.281766624007293 43 23.616017426061955 25.114172262057117 26.482157570870076 24.787652741010852 44 23.579859224066897 25.8234208727056 26.14007906593503 24.456640837292472 45 23.517951999439 25.798000560999984 25.063550779264038 25.620496660296983 46 23.64877894847566 26.576168896057222 25.429077330341332 24.345974825125786 47 23.172805525849828 25.565163654213634 26.70513314983959 24.556897670096948 48 22.982701039603093 25.060701951228065 27.223838993005035 24.73275801616381 49 23.347570168825932 24.98356445363861 27.09388860644098 24.574976771094477 50 21.771072561841482 26.000486492172296 27.41251906523378 24.81592188075244 51 22.731785031819218 25.212456829298226 25.285430655142793 26.770327483739763 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 432.0 1 914.0 2 1396.0 3 2445.5 4 3495.0 5 2624.0 6 1753.0 7 1660.0 8 1567.0 9 1586.0 10 1605.0 11 1630.0 12 1655.0 13 1747.0 14 1839.0 15 1799.5 16 1760.0 17 1784.5 18 1809.0 19 2096.5 20 2384.0 21 2760.5 22 3137.0 23 2953.5 24 2770.0 25 3584.0 26 5889.5 27 7381.0 28 8619.5 29 9858.0 30 11804.5 31 13751.0 32 15456.5 33 17162.0 34 19108.0 35 21054.0 36 22571.0 37 24088.0 38 26641.5 39 29195.0 40 32863.0 41 36531.0 42 41584.0 43 46637.0 44 51736.5 45 56836.0 46 62303.0 47 67770.0 48 70240.0 49 72710.0 50 70391.5 51 68073.0 52 61372.0 53 54671.0 54 52040.0 55 49409.0 56 50355.5 57 51302.0 58 48638.5 59 45975.0 60 46537.0 61 47099.0 62 45149.5 63 43200.0 64 38186.5 65 33173.0 66 28893.5 67 24614.0 68 21755.0 69 18896.0 70 17681.0 71 16466.0 72 12787.5 73 9109.0 74 8175.5 75 6055.0 76 4868.0 77 3699.5 78 2531.0 79 1945.0 80 1359.0 81 1164.0 82 969.0 83 645.0 84 321.0 85 249.5 86 178.0 87 127.0 88 76.0 89 55.5 90 35.0 91 67.0 92 99.0 93 56.0 94 13.0 95 9.0 96 5.0 97 2.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 912656.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.81151124741921 #Duplication Level Percentage of deduplicated Percentage of total 1 74.45919570462094 16.24067584585128 2 9.794508984940226 4.272660857759445 3 3.3516181816411432 2.193115729977615 4 1.599152019233346 1.3951968901536507 5 1.008660842734474 1.1000208658067159 6 0.6691824327305151 0.8757528094846189 7 0.5023287566001092 0.7669584527139773 8 0.4274499198292032 0.7458662987250485 9 0.3240602792705223 0.6361419983535727 >10 5.422730626005903 30.38363134021498 >50 2.178676187829925 32.87553349641513 >100 0.2553294786761438 7.134343959545526 >500 0.005076132776861706 0.7753590444728545 >1k 0.002030453110744682 0.6047424105255935 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1771 0.1940490173734682 No Hit GCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC 1481 0.16227362774144913 TruSeq Adapter, Index 16 (95% over 21bp) CCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC 1126 0.12337616801949476 TruSeq Adapter, Index 16 (95% over 21bp) GAATGATACGGCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGC 1084 0.11877421503830579 No Hit GAATCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTC 967 0.10595448887642223 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCG 943 0.10332480145859996 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 4.3828123630371135E-4 0.0 0.0 0.10179081713153697 0.0 2 4.3828123630371135E-4 0.0 0.0 0.5355796707631353 0.0 3 4.3828123630371135E-4 0.0 0.0 0.7650198979681282 0.0 4 4.3828123630371135E-4 0.0 0.0 1.0420136393120738 0.0 5 4.3828123630371135E-4 0.0 0.0 1.8658727932539751 0.0 6 4.3828123630371135E-4 0.0 0.0 2.321137427464455 0.0 7 4.3828123630371135E-4 0.0 0.0 2.788345225364212 0.0 8 4.3828123630371135E-4 0.0 0.0 3.5933582861450537 0.0 9 4.3828123630371135E-4 0.0 0.0 3.8973063235216774 0.0 10 4.3828123630371135E-4 0.0 0.0 4.6108281762241194 0.0 11 4.3828123630371135E-4 0.0 0.0 5.394584597044231 0.0 12 4.3828123630371135E-4 0.0 0.0 6.122350589926544 0.0 13 4.3828123630371135E-4 0.0 0.0 6.376553706982697 0.0 14 4.3828123630371135E-4 0.0 0.0 6.4781253834960815 0.0 15 4.3828123630371135E-4 0.0 0.0 6.686199400431269 0.0 16 5.478515453796393E-4 0.0 0.0 7.088212864430848 0.0 17 5.478515453796393E-4 0.0 0.0 7.58862046598061 0.0 18 5.478515453796393E-4 0.0 0.0 8.14019740186883 0.0 19 5.478515453796393E-4 0.0 0.0 8.420039971248752 0.0 20 5.478515453796393E-4 0.0 0.0 8.730890938097158 0.0 21 5.478515453796393E-4 0.0 0.0 9.085679598885013 0.0 22 5.478515453796393E-4 0.0 0.0 9.478379586613137 0.0 23 5.478515453796393E-4 0.0 0.0 9.872723128977402 0.0 24 5.478515453796393E-4 0.0 0.0 10.215130344839677 0.0 25 5.478515453796393E-4 0.0 0.0 10.507683070072405 0.0 26 5.478515453796393E-4 0.0 0.0 10.770103960309251 0.0 27 5.478515453796393E-4 0.0 0.0 11.030662155291807 0.0 28 5.478515453796393E-4 0.0 0.0 11.307546326326678 0.0 29 5.478515453796393E-4 0.0 0.0 11.601085184341088 0.0 30 5.478515453796393E-4 0.0 0.0 11.934288494240985 0.0 31 5.478515453796393E-4 0.0 0.0 12.232758016163812 0.0 32 6.57421854455567E-4 0.0 0.0 12.539335740958258 0.0 33 6.57421854455567E-4 0.0 0.0 12.819507021265405 0.0 34 6.57421854455567E-4 0.0 0.0 13.107129082589717 0.0 35 6.57421854455567E-4 0.0 0.0 13.415240791711225 0.0 36 6.57421854455567E-4 0.0 0.0 13.729817149068214 0.0 37 6.57421854455567E-4 0.0 0.0 14.038586280044179 0.0 38 6.57421854455567E-4 0.0 0.0 14.34395873143879 0.0 39 8.765624726074227E-4 0.0 0.0 14.659521221577462 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGAATA 20 7.0325215E-4 45.000004 22 TATTGCG 40 6.8121153E-9 45.000004 1 CCGTATA 20 7.0325215E-4 45.000004 20 ATATACG 20 7.0325215E-4 45.000004 1 GCGATAT 40 6.8121153E-9 45.000004 9 TGTACGT 20 7.0325215E-4 45.000004 31 CGTCCAT 20 7.0325215E-4 45.000004 19 AATACGG 40 6.8121153E-9 45.000004 2 TAATCGT 20 7.0325215E-4 45.000004 21 ATTACGT 25 3.8901722E-5 45.0 16 ATAGTAG 65 0.0 41.53846 1 GTATACG 55 6.002665E-11 40.909092 1 TATAGCG 90 0.0 40.0 1 CCCGTAA 40 3.4571713E-7 39.375004 33 CCGTAAA 40 3.4571713E-7 39.375004 34 ACAATCG 40 3.4571713E-7 39.375004 13 TTACGTC 40 3.4571713E-7 39.375004 17 CGATATG 40 3.4571713E-7 39.375004 10 CCGTTCG 40 3.4571713E-7 39.375004 13 GCCCGTA 40 3.4571713E-7 39.375004 32 >>END_MODULE