SRR3550235_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550235_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	987930
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5395	0.5460913222596743	No Hit
GCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCTGC	1684	0.17045742107234318	Illumina PCR Primer Index 6 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCTGC	1296	0.13118338343809785	Illumina PCR Primer Index 6 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCTGCT	1290	0.13057605295921776	Illumina PCR Primer Index 6 (95% over 24bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGTA	25	3.8904072E-5	45.0	34
TAGCGGA	35	1.2117016E-7	45.0	3
TACGATC	25	3.8904072E-5	45.0	11
AATTGCG	20	7.0328056E-4	45.0	19
GAACGAC	20	7.0328056E-4	45.0	30
CTCGATA	20	7.0328056E-4	45.0	27
ATCGGTT	20	7.0328056E-4	45.0	26
ATACGAT	25	3.8904072E-5	45.0	10
CGGTACG	20	7.0328056E-4	45.0	19
TCGACAC	40	6.8139343E-9	45.0	34
TATCGCA	20	7.0328056E-4	45.0	1
TCGACGT	30	2.1651922E-6	44.999996	26
CGAAAGG	60	3.6379788E-12	41.249996	2
CCGTAGG	50	1.0804797E-9	40.5	1
CGTTTTT	2270	0.0	40.44053	1
TTAACGG	85	0.0	39.705883	2
CGATTAT	40	3.4575714E-7	39.375	10
ACGTATG	40	3.4575714E-7	39.375	1
ACGTATA	40	3.4575714E-7	39.375	32
CGATAGT	40	3.4575714E-7	39.375	12