##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550235_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	987930
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06302470822832	33.0	31.0	34.0	30.0	34.0
2	32.245254218416285	34.0	31.0	34.0	30.0	34.0
3	32.19334365795147	34.0	31.0	34.0	30.0	34.0
4	35.80458534511555	37.0	35.0	37.0	35.0	37.0
5	35.83263186663023	37.0	35.0	37.0	35.0	37.0
6	35.71448381970382	37.0	35.0	37.0	35.0	37.0
7	36.03530513295476	37.0	35.0	37.0	35.0	37.0
8	36.055682082738656	37.0	35.0	37.0	35.0	37.0
9	37.699654833844505	39.0	38.0	39.0	35.0	39.0
10	37.328207464091584	39.0	37.0	39.0	34.0	39.0
11	37.15756480722318	39.0	37.0	39.0	34.0	39.0
12	36.975187513285356	39.0	37.0	39.0	33.0	39.0
13	36.90085026267043	39.0	37.0	39.0	33.0	39.0
14	38.02149646229996	40.0	37.0	41.0	33.0	41.0
15	38.10001113439211	40.0	37.0	41.0	33.0	41.0
16	38.17204862692701	40.0	37.0	41.0	33.0	41.0
17	38.16137580597815	40.0	37.0	41.0	34.0	41.0
18	38.10439909710202	40.0	37.0	41.0	33.0	41.0
19	38.00895002682376	40.0	37.0	41.0	34.0	41.0
20	37.817452653528086	40.0	36.0	41.0	33.0	41.0
21	37.70624943062768	40.0	36.0	41.0	33.0	41.0
22	37.68063627989837	39.0	36.0	41.0	33.0	41.0
23	37.68691101596267	39.0	36.0	41.0	33.0	41.0
24	37.693926695211196	39.0	36.0	41.0	33.0	41.0
25	37.61202210682943	39.0	35.0	41.0	33.0	41.0
26	37.53254785258065	39.0	35.0	41.0	33.0	41.0
27	37.46351057261142	39.0	35.0	41.0	33.0	41.0
28	37.352551294120026	39.0	35.0	41.0	33.0	41.0
29	37.29189618697681	39.0	35.0	41.0	32.0	41.0
30	37.22278906400251	39.0	35.0	41.0	32.0	41.0
31	37.058872592187704	39.0	35.0	41.0	32.0	41.0
32	36.87582419807072	39.0	35.0	41.0	31.0	41.0
33	36.71949530837205	39.0	35.0	41.0	31.0	41.0
34	36.585193282924905	39.0	35.0	41.0	30.0	41.0
35	36.502470822831576	39.0	35.0	41.0	30.0	41.0
36	36.37739414735862	39.0	35.0	41.0	30.0	41.0
37	36.28169404714909	39.0	35.0	41.0	30.0	41.0
38	36.16538115048637	39.0	35.0	40.0	30.0	41.0
39	36.04018604557003	38.0	35.0	40.0	29.0	41.0
40	35.91186521312239	38.0	35.0	40.0	29.0	41.0
41	35.86096788233984	38.0	35.0	40.0	28.0	41.0
42	35.77288876742279	38.0	35.0	40.0	28.0	41.0
43	35.68652738554351	38.0	35.0	40.0	28.0	41.0
44	35.583592967113056	38.0	34.0	40.0	28.0	41.0
45	35.39200246981061	38.0	34.0	40.0	27.0	41.0
46	35.347361655178	38.0	34.0	40.0	27.0	41.0
47	35.28082455234683	38.0	34.0	40.0	27.0	41.0
48	35.19348638061401	38.0	34.0	40.0	26.0	41.0
49	35.09115321935764	37.0	34.0	40.0	26.0	41.0
50	34.91336228275283	37.0	34.0	40.0	26.0	41.0
51	33.50761389977023	36.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	10.0
9	21.0
10	21.0
11	11.0
12	19.0
13	15.0
14	20.0
15	31.0
16	67.0
17	126.0
18	264.0
19	509.0
20	892.0
21	1471.0
22	2400.0
23	3625.0
24	5369.0
25	7660.0
26	9863.0
27	11677.0
28	13392.0
29	15717.0
30	18750.0
31	23401.0
32	29853.0
33	39848.0
34	66647.0
35	85830.0
36	81153.0
37	114987.0
38	187092.0
39	267128.0
40	61.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.90890042816799	21.405261506382033	26.428188231959755	12.257649833490227
2	31.948923506726185	24.25030113469578	26.56817790734161	17.232597451236423
3	31.37054244733939	23.97082789266446	27.900357312765074	16.758272347231078
4	27.86422114927171	25.80162562124847	27.522395311408705	18.811757918071116
5	24.87149899284362	30.434038848906297	25.49775793831547	19.19670421993461
6	26.0470883564625	34.36812324759851	24.42065733402164	15.164131061917344
7	82.11442106222101	5.409695018877856	8.102902027471583	4.372981891429554
8	83.64013644691425	4.420151225289241	7.6314111323676785	4.308301195428825
9	77.62139017946616	7.066998673995122	9.744516311884444	5.567094834654277
10	41.664794064356784	28.980798234692738	16.10144443432227	13.252963266628203
11	30.467846912230623	25.391373882764974	25.24753778101687	18.89324142398753
12	27.585861346451672	22.362718006336483	29.379207028838078	20.67221361837377
13	24.470458433289807	24.313969613231706	30.82627311651635	20.389298836962134
14	20.788922292065227	27.958762260484043	29.85909932889982	21.39321611855091
15	19.84786371504054	26.167542234773716	33.726174931422264	20.258419118763474
16	22.572348243296588	25.065844746085247	32.11371250999564	20.248094500622514
17	22.71972710617149	24.34767645480955	28.696466348830384	24.236130090188578
18	23.14030346279595	25.22061279645319	29.76415333070157	21.874930410049295
19	24.978794044112437	26.908384197260936	26.567773020355695	21.545048738270932
20	25.86509165629144	26.841881509823573	26.8994766835707	20.393550150314294
21	25.091453847944695	26.101241990829312	27.916552792201877	20.890751369024123
22	23.45611531181359	24.93871023250635	27.479477290901176	24.12569716477888
23	23.114087030457622	27.273997145546748	27.10768981608009	22.50422600791554
24	23.031085198344012	25.44704584332898	29.296407640217424	22.22546131810958
25	22.52487524419746	27.442531353435974	26.799469598048443	23.23312380431812
26	22.101161013432126	28.32103489113601	26.87275414249998	22.705049952931887
27	23.97902685412934	26.7445061897098	27.639711315579042	21.63675564058182
28	21.31537659550778	27.20051015760226	28.672071907928697	22.81204133896126
29	23.01529460589313	25.86701486947456	27.87171155851123	23.24597896612108
30	24.472685311712368	25.36465134169425	27.63920520684664	22.523458139746744
31	25.083760995212213	27.10455194193921	24.965533995323554	22.846153067525027
32	25.609709189922363	28.16353385361311	25.125869241747896	21.100887714716627
33	24.342919032724993	27.19636006599658	25.360602471835048	23.10011842944338
34	22.340145556871438	28.18357575941615	27.06082414745984	22.415454536252568
35	23.37230370572814	27.64568339862136	26.09982488637859	22.88218800927191
36	23.937930825058455	28.280748636036968	26.0248195722369	21.75650096666768
37	23.2306944824026	28.941422975312015	26.308442905873896	21.519439636411487
38	21.652647454779185	29.293573431315984	25.83270069741783	23.221078416487
39	22.36960108509712	27.170852185883614	27.00160942576903	23.457937303250233
40	23.248509509783084	26.53406617877785	28.53471399795532	21.68271031348375
41	21.09177775753343	27.310740639518993	27.27915945461723	24.31832214833035
42	23.410869191137024	26.41867338779063	26.136770823843797	24.03368659722855
43	23.07612887552762	26.38162622857895	26.767483526160763	23.774761369732673
44	22.572145799803632	26.386079985424065	26.636401364469144	24.40537285030316
45	21.981010800360348	26.769609182836838	26.636401364469144	24.612978652333666
46	23.116111465387224	26.97761987185327	26.336177664409423	23.570090998350086
47	21.610539208243498	27.56581944064863	27.597603069043352	23.22603828206452
48	21.880801271345135	25.88968854068608	28.13660886904943	24.092901318919356
49	22.08263743382628	25.62772666079581	28.202706669500877	24.086929235877037
50	21.494134199791485	25.23387284524207	29.074833237172676	24.19715971779377
51	20.74063951899426	26.331926351057263	27.128136608869045	25.799297521079428
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	382.0
1	788.0
2	1194.0
3	4546.5
4	7899.0
5	5414.0
6	2929.0
7	2702.5
8	2476.0
9	2512.5
10	2549.0
11	2713.5
12	2878.0
13	2933.0
14	2988.0
15	2940.5
16	2893.0
17	2775.0
18	2657.0
19	2779.5
20	2902.0
21	3436.0
22	3970.0
23	3719.5
24	3469.0
25	4451.0
26	6359.0
27	7285.0
28	8792.0
29	10299.0
30	12650.5
31	15002.0
32	15994.0
33	16986.0
34	19795.5
35	22605.0
36	25808.0
37	29011.0
38	31916.5
39	34822.0
40	39729.5
41	44637.0
42	48808.0
43	52979.0
44	60222.5
45	67466.0
46	78501.0
47	89536.0
48	94103.5
49	98671.0
50	94188.0
51	89705.0
52	79629.5
53	69554.0
54	61790.5
55	54027.0
56	50692.0
57	47357.0
58	44976.5
59	42596.0
60	40706.5
61	38817.0
62	35133.5
63	31450.0
64	27969.5
65	24489.0
66	20722.5
67	16956.0
68	14871.0
69	12786.0
70	11159.5
71	9533.0
72	8270.0
73	7007.0
74	5795.5
75	3354.0
76	2124.0
77	1794.0
78	1464.0
79	1198.0
80	932.0
81	629.0
82	326.0
83	239.0
84	152.0
85	118.5
86	85.0
87	58.5
88	32.0
89	26.5
90	21.0
91	13.5
92	6.0
93	4.5
94	3.0
95	3.5
96	4.0
97	3.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	987930.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.37386570048063
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.13250361487854	19.064020564648036
2	9.942782209328538	5.0457364093726085
3	3.5491087705953794	2.701638279044553
4	1.744015232948477	1.7700963320170842
5	1.0903632400580037	1.3833365208986355
6	0.7916077843783654	1.2051689764963007
7	0.5706328926636024	1.013541366790612
8	0.44404518072731447	0.9013714224576422
9	0.3568191565219917	0.8148493221253016
>10	4.546976534185503	27.352521658603514
>50	1.5209116762032862	27.525603686317822
>100	0.30700630873322315	9.937645979544355
>500	0.0016136993888737094	0.29511679277125674
>1k	0.001210274541655282	0.4370963250548964
>5k	4.0342484721842735E-4	0.5522563638574159
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5395	0.5460913222596743	No Hit
GCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCTGC	1684	0.17045742107234318	Illumina PCR Primer Index 6 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCTGC	1296	0.13118338343809785	Illumina PCR Primer Index 6 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCTGCT	1290	0.13057605295921776	Illumina PCR Primer Index 6 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0122174648001376E-4	0.0	0.0	0.161752350875062	0.0
2	2.0244349296002752E-4	0.0	0.0	0.6886115413035336	0.0
3	2.0244349296002752E-4	0.0	0.0	1.0327654793355805	0.0
4	2.0244349296002752E-4	0.0	0.0	1.4287449515653943	0.0
5	2.0244349296002752E-4	0.0	0.0	2.515056734788902	0.0
6	2.0244349296002752E-4	0.0	0.0	3.291832417276528	0.0
7	2.0244349296002752E-4	0.0	0.0	4.054740720496391	0.0
8	2.0244349296002752E-4	0.0	0.0	5.333879930764326	0.0
9	2.0244349296002752E-4	0.0	0.0	5.861650116911117	0.0
10	2.0244349296002752E-4	0.0	0.0	6.749769720526758	0.0
11	2.0244349296002752E-4	0.0	0.0	7.905114734849635	0.0
12	2.0244349296002752E-4	0.0	0.0	8.792728229732877	0.0
13	2.0244349296002752E-4	0.0	0.0	9.148117781624205	0.0
14	2.0244349296002752E-4	0.0	0.0	9.288917230977903	0.0
15	2.0244349296002752E-4	0.0	0.0	9.516261273572015	0.0
16	2.0244349296002752E-4	0.0	0.0	9.985525290253358	0.0
17	3.036652394400413E-4	0.0	0.0	10.59417165183768	0.0
18	3.036652394400413E-4	0.0	0.0	11.269624366098812	0.0
19	3.036652394400413E-4	0.0	0.0	11.665603838328627	0.0
20	3.036652394400413E-4	0.0	0.0	12.031722895346837	0.0
21	3.036652394400413E-4	0.0	0.0	12.524369135465063	0.0
22	3.036652394400413E-4	0.0	0.0	13.039790268541294	0.0
23	3.036652394400413E-4	0.0	0.0	13.531424291194721	0.0
24	3.036652394400413E-4	0.0	0.0	13.952709200044538	0.0
25	3.036652394400413E-4	0.0	0.0	14.292409381231463	0.0
26	3.036652394400413E-4	0.0	0.0	14.621987387770389	0.0
27	4.0488698592005504E-4	0.0	0.0	14.948022633182513	0.0
28	4.0488698592005504E-4	0.0	0.0	15.298857206482241	0.0
29	4.0488698592005504E-4	0.0	0.0	15.655765084570769	0.0
30	4.0488698592005504E-4	0.0	0.0	16.11328737866043	0.0
31	4.0488698592005504E-4	0.0	0.0	16.494488475904163	0.0
32	4.0488698592005504E-4	0.0	0.0	16.869413824866133	0.0
33	4.0488698592005504E-4	0.0	0.0	17.239885416982986	0.0
34	4.0488698592005504E-4	0.0	0.0	17.604182482564553	0.0
35	4.0488698592005504E-4	0.0	0.0	17.990647110625247	0.0
36	4.0488698592005504E-4	0.0	0.0	18.39421821384106	0.0
37	4.0488698592005504E-4	0.0	0.0	18.774811980605914	0.0
38	4.0488698592005504E-4	0.0	0.0	19.164414482807487	0.0
39	4.0488698592005504E-4	0.0	0.0	19.55594019819218	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGTA	25	3.8904072E-5	45.0	34
TAGCGGA	35	1.2117016E-7	45.0	3
TACGATC	25	3.8904072E-5	45.0	11
AATTGCG	20	7.0328056E-4	45.0	19
GAACGAC	20	7.0328056E-4	45.0	30
CTCGATA	20	7.0328056E-4	45.0	27
ATCGGTT	20	7.0328056E-4	45.0	26
ATACGAT	25	3.8904072E-5	45.0	10
CGGTACG	20	7.0328056E-4	45.0	19
TCGACAC	40	6.8139343E-9	45.0	34
TATCGCA	20	7.0328056E-4	45.0	1
TCGACGT	30	2.1651922E-6	44.999996	26
CGAAAGG	60	3.6379788E-12	41.249996	2
CCGTAGG	50	1.0804797E-9	40.5	1
CGTTTTT	2270	0.0	40.44053	1
TTAACGG	85	0.0	39.705883	2
CGATTAT	40	3.4575714E-7	39.375	10
ACGTATG	40	3.4575714E-7	39.375	1
ACGTATA	40	3.4575714E-7	39.375	32
CGATAGT	40	3.4575714E-7	39.375	12
>>END_MODULE