Basic Statistics
Measure | Value |
---|---|
Filename | SRR3550232_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 947215 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 48 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC | 3301 | 0.3484953257708123 | Illumina Single End Adapter 2 (95% over 21bp) |
GCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC | 2885 | 0.3045771023474079 | Illumina Single End Adapter 2 (95% over 21bp) |
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 2134 | 0.2252920403498678 | No Hit |
CTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGCT | 1958 | 0.206711253516889 | Illumina Single End Adapter 2 (95% over 22bp) |
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGC | 1494 | 0.15772554277539946 | No Hit |
GAATGATACCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCG | 1381 | 0.1457958330474074 | No Hit |
GAATCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTC | 1154 | 0.12183084093896318 | No Hit |
TCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC | 1118 | 0.11803022545039932 | Illumina Single End Adapter 2 (95% over 21bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTATCGT | 20 | 7.0326583E-4 | 45.0 | 11 |
TTTCGCG | 20 | 7.0326583E-4 | 45.0 | 1 |
TAGCGTA | 20 | 7.0326583E-4 | 45.0 | 1 |
TAGAACG | 50 | 2.1827873E-11 | 45.0 | 1 |
CACGCTA | 25 | 3.890285E-5 | 45.0 | 19 |
GCACGAA | 20 | 7.0326583E-4 | 45.0 | 9 |
ATTCGGT | 20 | 7.0326583E-4 | 45.0 | 39 |
AAGCGTA | 20 | 7.0326583E-4 | 45.0 | 24 |
TCTAGCG | 40 | 6.8121153E-9 | 45.0 | 1 |
GCGCAAT | 20 | 7.0326583E-4 | 45.0 | 19 |
GCCGATA | 20 | 7.0326583E-4 | 45.0 | 9 |
CTAACGG | 40 | 6.8121153E-9 | 45.0 | 2 |
ATTCACG | 25 | 3.890285E-5 | 45.0 | 18 |
CTAGTCG | 20 | 7.0326583E-4 | 45.0 | 23 |
GTATGCG | 25 | 3.890285E-5 | 45.0 | 1 |
TCGCTAA | 25 | 3.890285E-5 | 45.0 | 20 |
ACTCGGT | 25 | 3.890285E-5 | 45.0 | 20 |
TCGTAAG | 45 | 3.8380676E-10 | 45.0 | 1 |
ACATGCG | 40 | 6.8121153E-9 | 45.0 | 1 |
CATCGTT | 25 | 3.890285E-5 | 45.0 | 36 |