FastQCFastQC Report
Sat 18 Jun 2016
SRR3550232_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550232_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences947215
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC33010.3484953257708123Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC28850.3045771023474079Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21340.2252920403498678No Hit
CTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGCT19580.206711253516889Illumina Single End Adapter 2 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGC14940.15772554277539946No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCG13810.1457958330474074No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTC11540.12183084093896318No Hit
TCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC11180.11803022545039932Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGT207.0326583E-445.011
TTTCGCG207.0326583E-445.01
TAGCGTA207.0326583E-445.01
TAGAACG502.1827873E-1145.01
CACGCTA253.890285E-545.019
GCACGAA207.0326583E-445.09
ATTCGGT207.0326583E-445.039
AAGCGTA207.0326583E-445.024
TCTAGCG406.8121153E-945.01
GCGCAAT207.0326583E-445.019
GCCGATA207.0326583E-445.09
CTAACGG406.8121153E-945.02
ATTCACG253.890285E-545.018
CTAGTCG207.0326583E-445.023
GTATGCG253.890285E-545.01
TCGCTAA253.890285E-545.020
ACTCGGT253.890285E-545.020
TCGTAAG453.8380676E-1045.01
ACATGCG406.8121153E-945.01
CATCGTT253.890285E-545.036